FlyBase Allele Report: Dmel\tkv[1AΔGS.UAS.Tag:HA]

General Information

Symbol

Dmel\tkv^{1AΔGS.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0092483

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UAS-tkv.1AΔGS}

Also Known As

tkvDN

Key Links

Transgenic product class

dominant_negative_variant

(Haerry et al., 1998)

inframe_deletion

(Haerry et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Haerry et al., 1998)

inframe_deletion

(Haerry et al., 1998)

Mutagen

in vitro construct

(Haerry et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-tkv.1AΔGS}

Cytology

Description

A dominant negative form of the tkv1 isoform, in which the GS box is deleted, tagged with Tag:HA, is expressed under the control of UAS regulatory sequences.

(Haerry et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tkv

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(McCabe et al., 2004)

visible, with Scer\GAL4^A9

(Haerry, 2010, Haerry et al., 1998)

visible, with Scer\GAL4^ap-md544

(Evans et al., 2009)

visible, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

visible, with Scer\GAL4^ptc-559.1

(Evans et al., 2009, Haerry et al., 1998)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^nub-AC-62

(Martin et al., 2004)

crossvein, with Scer\GAL4^A9

(Haerry et al., 1998)

dorsal row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

medial row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

neuromuscular junction, with Scer\GAL4^elav.PLu

(McCabe et al., 2004)

posterior crossvein, with Scer\GAL4^nub-AC-62

(Martin et al., 2004)

scutellar bristle | increased number, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

ventral row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

wing, with Scer\GAL4^A9

(Haerry et al., 1998)

wing, with Scer\GAL4^nub-AC-62

(Martin et al., 2004)

wing blade, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

wing disc, with Scer\GAL4^A9

(Haerry et al., 1998)

wing vein, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

wing vein L2, with Scer\GAL4^A9

(Haerry, 2010, Haerry et al., 1998)

wing vein L3, with Scer\GAL4^A9

(Haerry, 2010, Haerry et al., 1998)

wing vein L3, with Scer\GAL4^nub-AC-62

(Martin et al., 2004)

wing vein L3, with Scer\GAL4^ptc-559.1

(Haerry et al., 1998)

wing vein L4, with Scer\GAL4^A9

(Haerry, 2010, Haerry et al., 1998)

wing vein L4, with Scer\GAL4^ap-md544

(Evans et al., 2009)

wing vein L4, with Scer\GAL4^nub-AC-62

(Martin et al., 2004)

wing vein L4, with Scer\GAL4^ptc-559.1

(Haerry et al., 1998)

wing vein L5, with Scer\GAL4^A9

(Haerry et al., 1998)

Detailed Description

Statement

Reference

Expression of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 results in loss of wing vein L3 and L4, which merge with L2 and L5 respectively.

(Haerry, 2010)

Expression of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^Bx-MS1096 induces extensive defects in longitudinal wing vein formation and wing patterning.

Supernumerary scutellar bristles are induced in response to expression of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^ptc-559.1.

Scer\GAL4^ap-md544 driven expression of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} engenders large gaps in wing vein L4 in 91% of the wings.

(Evans et al., 2009)

tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1}; Scer\GAL4^nub-AC-62 flies have reduced wings lacking most vein material apart from L2 and L5.

(Martin et al., 2004)

Expression of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu in the neurons inhibits neuromuscular junction growth.

(McCabe et al., 2004)

Flies expressing tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} (carrying 3 to 4 copies of P{UAS-tkv.1AΔGS}) under the control of Scer\GAL4^A9 have small wings with partial loss of vein L4 and both cross veins. Veins L2 and L3 are closer together than normal and the triple row margin bristles are shifter more distally/posteriorly. Flies expressing higher levels of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} (carrying 6 to 8 copies of P{UAS-tkv.1AΔGS}) under the control of Scer\GAL4^A9 have very small wings that show fusion of veins L2 and L3 as well as L4 and L5. Wing discs of larvae expressing tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 are strongly reduced in size. Flies expressing tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ptc-559.1 show defects in wing veins L3 and L4.

(Haerry et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^A9, tkv^{1AΔGS.UAS.Tag:HA} has visible phenotype, enhanceable by Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA}

(Haerry, 2010)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, tkv^{1AΔGS.UAS.Tag:HA} has visible phenotype, suppressible by kek5^fe148/kek5^fe148

(Evans et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^A9, tkv^{1AΔGS.UAS.Tag:HA} has wing vein phenotype, enhanceable by Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA}

(Haerry, 2010)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, tkv^{1AΔGS.UAS.Tag:HA} has wing vein L4 phenotype, suppressible by kek5^fe148/kek5^fe148

(Evans et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in animals expressing tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 enhances the wing phenotype.

(Haerry, 2010)

A homozygous kek5^fe148 genetic background suppresses the wing vein L4 phenotypes engendered by Scer\GAL4^ap-md544 driven expression of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1}.

(Evans et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1}

tkv^{1AΔGS.UAS.T:HA1}

tkv^{1AΔGS.UAS.Tag:HA}

tkvDN

(Carneiro et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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