FlyBase Allele Report: Dmel\spn-B[BU]

General Information

Symbol

Dmel\spn-B^BU

Species

D. melanogaster

Name

FlyBase ID

FBal0092517

Feature type

allele

Associated gene

Dmel\spn-B

Associated Insertion(s)

Carried in Construct

Also Known As

spnB^BU

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Ghabrial et al., 1998)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: G107E.

(Ghabrial et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:14,328,651..14,328,651 [+]

Nucleotide change:

G14328651A

Amino acid change:

G107E | spn-B-PA

Comment:

site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change

(Ghabrial et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Bhagat et al., 2004)

abnormal planar polarity

(Abdu et al., 2007)

female sterile

(Lake et al., 2011, Liu et al., 2002)

female sterile | recessive

(Liu et al., 2000)

radiation sensitive

(Abdu et al., 2007)

semi-sterile

(Bhagat et al., 2004)

viable

(Ghabrial et al., 1998)

visible

(Li et al., 2014)

Phenotype Manifest In

dorsal appendage

(Lake et al., 2011, Abdu et al., 2007, Ghabrial et al., 1998)

dorsal appendage (with spn-B²)

(Bayer et al., 2020)

dorsal appendage | maternal effect

(Ferguson et al., 2012)

dorsal appendage | maternal effect (with spn-B²)

(Nagel et al., 2012)

dorsal appendage | maternal effect (with spn-B^Δ37C)

(Ferguson et al., 2012)

egg

(Abdu et al., 2007)

egg chorion

(Li et al., 2014)

egg chorion | maternal effect

(Ghabrial and Schupbach, 1999)

egg chorion | maternal effect (with spn-B²)

(Ghabrial and Schupbach, 1999)

karyosome

(Ferguson et al., 2012, Lake et al., 2011, Ghabrial and Schupbach, 1999)

nuclear membrane & oocyte nucleus

(Abdu et al., 2002)

oocyte nucleus

(Abdu et al., 2002)

oocyte nucleus & meiotic cell cycle

(McKim et al., 2000)

presumptive oocyte

(Joyce and McKim, 2009)

stage S1 oocyte | increased number

(Joyce and McKim, 2010)

Detailed Description

Statement

Reference

spn-B²/spn-B^BU but not spn-B^BU/+ females lay eggs with defective dorsal appendages (fused, narrowly spaced or missing) when compared to controls.

(Bayer et al., 2020)

On average, spn-B^BU mutant females produce only 11% wild-type looking eggs. More than 50% of their eggs are severely ventralised.

(Li et al., 2014)

The karyosome fails to condense properly in homozygous oocytes.

Eggs laid by homozygous and spn-B^BU/spn-B^Δ37C females display a range of ventralisation phenotypes, ranging from fusion at the base of the dorsal appendages through to complete lack of the appendages. The ventralised eggshell phenotypes caused by spn-B^BU can be suppressed by feeding the females rapamycin.

(Ferguson et al., 2012)

spn-B²/spn-B^BU females produce ventralised eggs that have fused dorsal appendages.

(Nagel et al., 2012)

spn-B^BU mutant females contain an increased number of meiotically-induced double strand breaks (DSBs) compared to controls.

Many spn-B^BU mutant dorsal appendages are abnormal compared to controls. Instead of spherical shaped karyosomes as is seen in wild-type stage 6 egg chambers, the karyosomes of spn-B^BU oocytes are fragmented. spn-B^BU mutants have significantly reduced fertility for three days after which time they become completely sterile.

(Lake et al., 2011)

Homozygous females show a high frequency (more than 60%) of region 3 cysts with two pro-oocytes (as assayed by c(3)G staining) compared to a frequency of only 9.5% in wild type.

(Joyce and McKim, 2009)

spn-B^BU mutants are sensitive to irradiation with 2500 rad, with only 19% of spn-B^BU mutants surviving.

Approximately 58% of eggs laid by spn-B^BU homozygous mutant mothers are abnormal, with ventralized eggshells exhibiting partially or completely fused appendages or lack appendages altogether.

(Abdu et al., 2007)

spn-B^BU/spn-B^BU females exhibit low fertility, and meioses show reduced frequency of crossing over on chromosome 2, although the reduction is less severe in the centromeric regions, as compared to wild type.

(Bhagat et al., 2004)

spn-B^BU animals show the same survival rate after exposure to X rays or to 0.08% methyl methanesulfonate as control animals.

(Abdu et al., 2003)

In mutant egg chambers, the oocyte nuclear DNA is found in a variety of conformations, as compared to the round shape seen in wild-type. 17% are wild-type in shape, 34% are oblong, the rest appear in small distinct clumps. In about 90% of the egg chambers, the DNA seems to be attached to the oocyte nuclear membrane. In addition the morphology of the oocyte nuclear membrane in the mutant egg chambers is also found in a variety of shapes.

(Abdu et al., 2002)

Homozygous females are sterile after the first 2-3 days of mating.

(Liu et al., 2002)

Homozygous females are fertile in the first 2 to 3 days of mating producing only a few progeny.

(Liu et al., 2000)

Precocious anaphases are seen in homozygous spn-B^BU females. Anaphase bridges are sometimes seen.

(McKim et al., 2000)

Homozygous and hemizygous spn-B^BU/Df(3R)trxE12 females produce eggs with a range of eggshell phenotypes; from resembling wild-type with two normal dorsal appendages, having two dorsal appendages fused at the base, having a single dorsal appendage to having little or no dorsal appendage material. Hemizygotes are viable. spn-B²/spn-B^BU larvae show normal sensitivity to methyl methanesulfonate. Recombination frequency is 10-25% of normal levels in homozygous and spn-B²/spn-B^BU females, and X chromosome non-disjunction is increased 100-fold compared to wild-type.

(Ghabrial et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

spn-B^BU has female sterile | recessive phenotype, enhanceable by mei-218¹

(Liu et al., 2000)

spn-B^BU has female sterile | recessive phenotype, enhanceable by mei-217^g10

(Liu et al., 2000)

spn-B^BU has female sterile | recessive phenotype, enhanceable by mei-217^r1

(Liu et al., 2000)

Suppressed by

Statement

Reference

spn-B^BU has visible phenotype, suppressible by eIF1A^BH167/eIF-1A[+]

(Li et al., 2014)

spn-B^BU has visible phenotype, suppressible by eIF-1A[+]/eIF1A⁶⁴⁵

(Li et al., 2014)

spn-B^BU has visible phenotype, suppressible by eIF1A²²³²/eIF-1A[+]

(Li et al., 2014)

spn-B^BU has visible phenotype, suppressible by eIF1A^EP935/eIF-1A[+]

(Li et al., 2014)

spn-B^BU has visible phenotype, suppressible by Df(3R)Cha7/+

(Li et al., 2014)

spn-B^BU has female sterile phenotype, suppressible by trem^F9

(Lake et al., 2011)

spn-B^BU has female sterile phenotype, suppressible by mei-W68¹

(Liu et al., 2002)

spn-B^BU has female sterile phenotype, suppressible by mei-P22^N1

(Liu et al., 2002)

spn-B^BU has female sterile | recessive phenotype, suppressible by mei-W68¹

(Liu et al., 2000)

NOT Suppressor of

Statement

Reference

spn-B^BU is a non-suppressor of abnormal meiotic cell cycle phenotype of trem^F9

(Lake et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

spn-B²/spn-B^BU has dorsal appendage phenotype, enhanceable by Xrcc2^CC/Xrcc2^CC

(Bayer et al., 2020)

spn-B²/spn-B^BU has dorsal appendage | maternal effect phenotype, enhanceable | maternal effect by CycG^HR7/CycG^HR7

(Nagel et al., 2012)

Suppressed by

Statement

Reference

spn-B^BU has egg chorion phenotype, suppressible by eIF1A^BH167/eIF-1A[+]

(Li et al., 2014)

spn-B^BU has egg chorion phenotype, suppressible by eIF-1A[+]/eIF1A⁶⁴⁵

(Li et al., 2014)

spn-B^BU has egg chorion phenotype, suppressible by eIF1A²²³²/eIF-1A[+]

(Li et al., 2014)

spn-B^BU has egg chorion phenotype, suppressible by eIF1A^EP935/eIF-1A[+]

(Li et al., 2014)

spn-B^BU has egg chorion phenotype, suppressible by Df(3R)Cha7/+

(Li et al., 2014)

spn-B^BU/spn-B^Δ37C has dorsal appendage | maternal effect phenotype, suppressible by Lnk^f05062/Lnk^CR642

(Ferguson et al., 2012)

spn-B^BU has dorsal appendage | maternal effect phenotype, suppressible by mei-P22^CA1215

(Ferguson et al., 2012)

spn-B^BU has dorsal appendage | maternal effect phenotype, suppressible by Lnk^CR642

(Ferguson et al., 2012)

spn-B^BU has dorsal appendage | maternal effect phenotype, suppressible by Lnk^CR642/Df(3R)Espl3

(Ferguson et al., 2012)

spn-B^BU has dorsal appendage | maternal effect phenotype, suppressible by Df(3R)BSC140/Lnk^CR642

(Ferguson et al., 2012)

spn-B^BU has dorsal appendage | maternal effect phenotype, suppressible by Lnk^d07478/Lnk^CR642

(Ferguson et al., 2012)

spn-B^BU has karyosome phenotype, suppressible by mei-P22^CA1215

(Ferguson et al., 2012)

spn-B^BU has dorsal appendage phenotype, suppressible by trem^F9

(Lake et al., 2011)

spn-B^BU has karyosome phenotype, suppressible by trem^F9

(Lake et al., 2011)

spn-B^BU has stage S1 oocyte | increased number phenotype, suppressible by Sirt1¹⁷/Df(2L)BSC245

(Joyce and McKim, 2010)

spn-B^BU has presumptive oocyte phenotype, suppressible by mei-218¹

(Joyce and McKim, 2009)

spn-B^BU has dorsal appendage phenotype, suppressible by Hus1-like³⁷/Hus1-like[+]

(Abdu et al., 2007)

spn-B^BU has egg phenotype, suppressible by Hus1-like³⁷/Hus1-like[+]

(Abdu et al., 2007)

spn-B^BU has karyosome phenotype, suppressible by Hus1-like³⁷/Hus1-like[+]

(Abdu et al., 2007)

spn-B^BU has dorsal appendage phenotype, suppressible by Hus1-like³⁷

(Abdu et al., 2007)

spn-B^BU has egg phenotype, suppressible by Hus1-like³⁷

(Abdu et al., 2007)

spn-B^BU has karyosome phenotype, suppressible by Hus1-like³⁷

(Abdu et al., 2007)

spn-B^BU has egg chorion phenotype, suppressible by Df(2L)pr-A14/lok^p6

(Abdu et al., 2002)

spn-B^BU has dorsal appendage phenotype, suppressible by Df(2L)pr-A14/lok^p6

(Abdu et al., 2002)

spn-B^BU has oocyte nucleus phenotype, suppressible by Df(2L)pr-A14/lok^p6

(Abdu et al., 2002)

spn-B²/spn-B^BU has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-W68^unspecified/Df(2R)LL5

(Ghabrial and Schupbach, 1999)

spn-B^BU has egg chorion | maternal effect phenotype, suppressible by mei-41^D3/mei-41^RT1

(Ghabrial and Schupbach, 1999)

spn-B^BU has egg chorion | maternal effect phenotype, suppressible by mei-41[+]/mei-41^D3

(Ghabrial and Schupbach, 1999)

spn-B^BU has egg chorion | maternal effect phenotype, suppressible by mei-41[+]/mei-41^RT1

(Ghabrial and Schupbach, 1999)

spn-B^BU has karyosome phenotype, suppressible by mei-41^unspecified

(Ghabrial and Schupbach, 1999)

NOT suppressed by

Statement

Reference

spn-B^BU has karyosome phenotype, non-suppressible by Lnk^CR642

(Ferguson et al., 2012)

spn-B^BU has egg chorion phenotype, non-suppressible by grp⁰⁶⁰³⁴

(Abdu et al., 2002)

spn-B^BU has egg chorion phenotype, non-suppressible by mus304^D1

(Abdu et al., 2002)

spn-B^BU has egg chorion phenotype, non-suppressible by mus304^D1/mus304⁶

(Abdu et al., 2002)

spn-B^BU has egg chorion phenotype, non-suppressible by Wee1^ES1

(Abdu et al., 2002)

spn-B^BU has dorsal appendage phenotype, non-suppressible by grp⁰⁶⁰³⁴

(Abdu et al., 2002)

spn-B^BU has dorsal appendage phenotype, non-suppressible by mus304^D1/mus304⁶

(Abdu et al., 2002)

spn-B^BU has dorsal appendage phenotype, non-suppressible by Wee1^ES1

(Abdu et al., 2002)

spn-B^BU has dorsal appendage phenotype, non-suppressible by mus304^D1

(Abdu et al., 2002)

Enhancer of

Statement

Reference

spn-B²/spn-B^BU is an enhancer | maternal effect of dorsal appendage | maternal effect phenotype of CycG^HR7

(Nagel et al., 2012)

Other

Statement

Reference

Xrcc2^CC, spn-B^BU has dorsal appendage phenotype

(Bayer et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xrcc2^CC homozygous females in the heterozygous background of spn-B^BU lay eggs with defective dorsal appendages (fused, narrowly spaced, branched or shortened) when compared to controls.

(Bayer et al., 2020)

eIF-1A^BH167 dominantly suppresses the eggshell patterning defects in spn-B^BU mutants.

(Li et al., 2014)

The ventralisation phenotype seen in eggs laid by spn-B^BU females is suppressed if the females are also mutant for mei-P22^CA1215, Lnk^CR642, Lnk^CR642/Df(3R)Espl3, Lnk^CR642/Df(3R)BSC140 or Lnk^CR642/Lnk^d07478.

The ventralisation phenotype seen in eggs laid by spn-B^BU/spn-B^Δ37C females is suppressed if the females are also mutant for Lnk^CR642/Lnk^f05062.

The failure of karyosome condensation which is seen in spn-B^BU oocytes is suppressed by mei-P22^CA1215 but not by Lnk^CR642.

(Ferguson et al., 2012)

The ventralised eggshell phenotype of eggs derived from spn-B^BU CycG^HR7/spn-B² CycG^HR7 double mutant females is enhanced compared to the defects seen in eggs from spn-B^BU/spn-B² and CycG^HR7/CycG^HR7 single mutant females.

(Nagel et al., 2012)

trem^F9 partially suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in spn-B^BU mutant females.

The dorsal appendage and karyosome defects observed in spn-B^BU are almost fully suppressed in the background of trem^F9. The sterility seen in spn-B^BU females is also partially suppressed, with flies remaining fertile until at least day 10.

spn-B^BU does not suppress the reduced frequency of meiotic recombination seen in trem^F9 mutant females.

(Lake et al., 2011)

The high frequency of region 3 cysts containing two pro-oocytes that is seen in spn-B^BU homozygous females is suppressed by mei-218¹.

(Joyce and McKim, 2009)

A heterozygous Hus1-like³⁷ background partially suppresses the abnormal egg phenotype for eggs laid by spn-B^BU homozygous mutant mothers, with only 45% of eggs exhibiting partially or completely fused appendages or lacking appendages altogether, compared to 51% in okr^AA mutants.

A homozygous Hus1-like³⁷ background completely suppresses the abnormal egg phenotype for eggs laid by spn-B^BU homozygous mutant mothers.

A heterozygous Hus1-like³⁷ background has no effect on the abnormal karyosome phenotype associated with spn-B^BU homozygous mutants.

A homozygous Hus1-like³⁷ background has no effect on the abnormal karyosome phenotype associated with spn-B^BU homozygous mutants.

(Abdu et al., 2007)

spn-D¹ spn-B^BU double mutant animals show the same survival rate after exposure to X rays or to 0.08% methyl methanesulfonate as control animals.

(Abdu et al., 2003)

The addition of lok^p6/Df(2L)pr-A14 completely suppresses the dorsal ventralisation phenotypes seen eggs laid by Df(2L)pr-A14 mothers. The oocyte nucleus phenotypes usually seen are also suppressed.

(Abdu et al., 2002)

The eggshell patterning defects of eggs derived from spn-B²/spn-B^BU females are suppressed if the females also carry mei-W68^unspecified/Df(2R)LL5. The eggshell patterning defects of eggs derived from spn-B^BU/spn-B^BU females are suppressed if the females also carry mei-41^D3/+, mei-41^RT1/+ or mei-41^D3/mei-41^RT1.

(Ghabrial and Schupbach, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

spn-B^unspecified

(Ghabrial et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

spn-B^BU

(Bayer et al., 2020, Alexander et al., 2016, Ferguson et al., 2012, Nagel et al., 2012, Lake et al., 2011, Joyce and McKim, 2009, Abdu et al., 2007)

spn-B^Bu

(Joyce and McKim, 2010)

spnB^BU

(Lake et al., 2015, Li et al., 2014, Bhagat et al., 2004, Jang et al., 2003, Liu et al., 2002, McKim et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

Open Close