Allele Dmel\sax^{Q263D.Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\sax^{Q263D.Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0092532
Feature type	allele	Associated gene	Dmel\sax

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Haerry et al., 1998) in vitro construct - regulatory fusion (Haerry et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An activated form of sax (with the amino acid replacement: Q263D), tagged with T:Ivir\HA1 is expressed under the control of Scer\UAS regulatory sequences. (Haerry et al., 1998)
Carried in construct	P{UAS-sax.Q263D.T:HA1} (Baines, 2004, Allan et al., 2003, Evans et al., 2009, Sutherland et al., 2003, Miguel-Aliaga et al., 2004, Haerry et al., 1998, Allan et al., 2003, Miller et al., 2011, Higashi-Kovtun et al., 2010, Haerry, 2010, Saja et al., 2010, Fischer et al., 2012)
Tagged with	T:Ivir\HA1 (Haerry et al., 1998, Allan et al., 2003, Allan et al., 2003, Sutherland et al., 2003, Miguel-Aliaga et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^A9 (Haerry et al., 1998, Haerry, 2010) visible \| recessive (Evans et al., 2009)
Phenotype Manifest In
	posterior crossvein, with Scer\GAL4^A9 (Fischer et al., 2012) wing, with Scer\GAL4^A9 (Haerry et al., 1998) wing cell (Evans et al., 2009) wing vein (Evans et al., 2009) wing vein, with Scer\GAL4^A9 (Haerry et al., 1998, Haerry, 2010) wing vein \| ectopic, with Scer\GAL4^A9 (Haerry et al., 1998, Fischer et al., 2012)
Detailed Description
	Statement Reference Overexpression of sax[Q263D.Scer\UAS.T:Ivir\HA1] with Scer\GAL4[A9] leads to extra vein material between the region of L3 and L5 with slight wing blistering, also affecting the posterior crossvein pattern. (Fischer et al., 2012) Pan-neuronal expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] does not generate appreciable motor axon phenotypes. (Miller et al., 2011) Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in the wing under the control of Scer\GAL4[A9] results in mild wing-vein patterning defects. (Haerry, 2010) Scer\GAL4[GMR.PF]-driven overexpression of sax[Q263D.Scer\UAS.T:Ivir\HA1] has no effect on eye development. (Saja et al., 2010) Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in the wing with Scer\GAL4[ptc-559.1] induces patterning abnormalities including extensive ectopic vein, fusion of longitudinal veins, and loss of intervein regions. (Evans et al., 2009) Expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1}, under the control of either Scer\GAL4^B19, or Scer\GAL4^eve.RN2 has no effect on the synaptic current amplitude in aCC/RP2 neurons compared to controls. (Baines, 2004) Flies expressing sax^{Q263D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 have ectopic and thickened wing veins with a small amount of wing blistering in the region of the posterior crossvein. (Haerry et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1} has visible phenotype, non-enhanceable by Mad^{SDVD.Scer\UAS}, Scer\GAL4^A9 (Haerry, 2010)
Enhancer of
Statement Reference sax^{Q263D.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF is an enhancer of neuroanatomy defective phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Saja et al., 2010) Scer\GAL4^A9/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is an enhancer of visible phenotype of Scer\GAL4^A9, tkv^{1-A-T318D.Scer\UAS} (Haerry, 2010) Scer\GAL4^A9/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is an enhancer of visible phenotype of Scer\GAL4^A9, tkv^{1-A-T485D.Scer\UAS} (Haerry, 2010) Scer\GAL4^A9/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is an enhancer of visible phenotype of Scer\GAL4^A9, tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} (Haerry, 2010)
Suppressor of
Statement Reference sax^{Q263D.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^how-24B, Scer\GAL4^how-24B is a suppressor \| partially of neuroanatomy defective \| embryonic stage phenotype of Scer\GAL4^how-24B/Scer\GAL4^how-24B, frac^Scer\UAS.cMa (Miller et al., 2011) sax^{Q263D.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^Rapgap1-OK6 is a suppressor of neurophysiology defective phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010) Scer\GAL4^elav-C155/sax^{Q263D.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^how-24B/Scer\GAL4^how-24B is a suppressor \| partially of neuroanatomy defective \| embryonic stage phenotype of Scer\GAL4^elav-C155, Scer\GAL4^how-24B/Scer\GAL4^how-24B, frac^Scer\UAS.cMa (Miller et al., 2011) Scer\GAL4^elav-C155/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is a suppressor \| partially of neuroanatomy defective \| embryonic stage phenotype of Scer\GAL4^elav-C155, frac^Scer\UAS.cMa (Miller et al., 2011) Scer\GAL4^elav-C155/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is a suppressor of neuroanatomy defective \| embryonic stage phenotype of frac^Δ1 (Miller et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^G14/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is a non-suppressor of neurophysiology defective phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010)
Other
Statement Reference kek5^{Scer\UAS.T:Avic\GFP}, sax^{Q263D.Scer\UAS.T:Ivir\HA1} has visible \| recessive phenotype (Evans et al., 2009) Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1}, tkv^{1-A-T318D-T485D.Scer\UAS} has visible phenotype (Haerry, 2010) Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1}, tkv^{DE-Q199D.tld.T:Ivir\HA1} has visible phenotype (Haerry et al., 1998, Haerry et al., 1998)
Phenotype Manifest In
Enhanced by
Statement Reference sax^{Q263D.Scer\UAS.T:Ivir\HA1} has wing cell phenotype, enhanceable by kek5^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009) sax^{Q263D.Scer\UAS.T:Ivir\HA1} has wing vein phenotype, enhanceable by kek5^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009)
NOT Enhanced by
Statement Reference Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1} has wing vein phenotype, non-enhanceable by Mad^{SDVD.Scer\UAS}, Scer\GAL4^A9 (Haerry, 2010)
Suppressed by
Statement Reference Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1} has posterior crossvein phenotype, suppressible \| partially by Scer\GAL4^A9/fuss^Scer\UAS.C (Fischer et al., 2012) Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1} has wing vein \| ectopic phenotype, suppressible \| partially by Scer\GAL4^A9/fuss^Scer\UAS.C (Fischer et al., 2012)
Enhancer of
Statement Reference sax^{Q263D.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Saja et al., 2010) Scer\GAL4^A9/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is an enhancer of wing phenotype of Scer\GAL4^A9, tkv^{1-A-T318D.Scer\UAS} (Haerry, 2010) Scer\GAL4^A9/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is an enhancer of wing vein phenotype of Scer\GAL4^A9, tkv^{1-A-T485D.Scer\UAS} (Haerry, 2010) Scer\GAL4^A9/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is an enhancer of wing vein phenotype of Scer\GAL4^A9, tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} (Haerry, 2010)
Suppressor of
Statement Reference sax^{Q263D.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^how-24B, Scer\GAL4^how-24B is a suppressor \| partially of intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype of Scer\GAL4^how-24B/Scer\GAL4^how-24B, frac^Scer\UAS.cMa (Miller et al., 2011) sax^{Q263D.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^Rapgap1-OK6 is a suppressor of bouton phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010) Scer\GAL4^elav-C155/sax^{Q263D.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^how-24B/Scer\GAL4^how-24B is a suppressor \| partially of intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype of Scer\GAL4^elav-C155, Scer\GAL4^how-24B/Scer\GAL4^how-24B, frac^Scer\UAS.cMa (Miller et al., 2011) Scer\GAL4^elav-C155/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is a suppressor \| partially of intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype of Scer\GAL4^elav-C155, frac^Scer\UAS.cMa (Miller et al., 2011) Scer\GAL4^elav-C155/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is a suppressor of intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype of frac^Δ1 (Miller et al., 2011) Scer\GAL4^elav-C155/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is a suppressor of segmental nerve branch SNa of A1-7 \| embryonic stage phenotype of frac^Δ1 (Miller et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^G14/sax^{Q263D.Scer\UAS.T:Ivir\HA1} is a non-suppressor of bouton phenotype of Ranbp11⁷⁰ (Higashi-Kovtun et al., 2010)
Other
Statement Reference Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1}, tkv^{DE-Q199D.tld.T:Ivir\HA1} has wing disc phenotype (Haerry et al., 1998, Haerry et al., 1998) Scer\GAL4^A9, sax^{Q263D.Scer\UAS.T:Ivir\HA1}, tkv^{DE-Q199D.tld.T:Ivir\HA1} has wing phenotype (Haerry et al., 1998, Haerry et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference Introducing one copy of fuss[Scer\UAS.C] almost completely rescues the sax[Q263D.Scer\UAS.T:Ivir\HA1], Scer\GAL4[A9] wing phenotype - vein formation is close to wild type. (Fischer et al., 2012) Pan-neuronal expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] reduces the incidence of defasciculation from 64% to 30% in frac[Δ1] mutants. Co-expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] partially suppresses the motor axon mis-projections and hypo-fasciculation found upon expression of frac[Scer\UAS.cMa] (from 55% to 27%, when both transgenes are expressed under the control of either or both Scer\GAL4[elav-C155] and Scer\GAL4[how-24B]). (Miller et al., 2011) Expression of Mad[SDVD.Scer\UAS] in animals expressing sax[Q263D.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[A9] does not enhance the wing vein patterning defects. Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in animals expressing tkv[1AΔGS.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[A9] enhances the wing phenotype. Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in animals expressing tkv[1-A-T318D.Scer\UAS] under the control of Scer\GAL4[A9] enhances the wing phenotype. Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in animals expressing tkv[1-A-T485D.Scer\UAS] under the control of Scer\GAL4[A9] slightly enhances the wing phenotype. Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in animals expressing tkv[1-A-T318D-T485D.Scer\UAS] under the control of Scer\GAL4[A9] results in a wing phenotype similar to co-expression with the single mutant tkv[1-A-T485D.Scer\UAS]. (Haerry, 2010) Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in motor neurons (under the control of Scer\GAL4[OK6]) rescues bouton number and EJP phenotypes in Ranbp11[70] mutants, although mEJP frequency is not rescued. Expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] in muscle (under the control of Scer\GAL4[G14]) does not rescue bouton number or mEJP and EJP phenotypes in Ranbp11[70] mutants. (Higashi-Kovtun et al., 2010) Overexpression of sax[Q263D.Scer\UAS.T:Ivir\HA1] enhances the adult visual system degeneration seen in Scer\GAL4[GMR.PF], Ppt1[Scer\UAS.cKa] flies. (Saja et al., 2010) The wing patterning phenotypes resulting from the expression of sax[Q263D.Scer\UAS.T:Ivir\HA1] with Scer\GAL4[ptc-559.1] are enhanced by the co-expression of kek5[Scer\UAS.T:Avic\GFP], and there is an additional decrease in wing size. (Evans et al., 2009) Flies expressing sax^{Q263D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 and also carrying tkv^{DE-Q199D.tld.T:Ivir\HA1} have blistered, darkly pigmented wings. Wings discs of these animals show overgrowth. (Haerry et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	sax^{Q263D.Scer\UAS.T:Ivir\HA1} sax^{Q263D.UAS.T:HA1} SAXA (Haerry et al., 1998)
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Fischer et al., 2012, PLoS ONE 7(8): e42349 fussel (fuss) - A Negative Regulator of BMP Signaling in Drosophila melanogaster. [FBrf0219160] Miller et al., 2011, J. Neurosci. 31(14): 5335--5347 Drosophila mmp2 regulates the matrix molecule faulty attraction (frac) to promote motor axon targeting in Drosophila. [FBrf0213409] Haerry, 2010, Mech. Dev. 127(7-8): 358--370 The interaction between two TGF-beta type I receptors plays important roles in ligand binding, SMAD activation, and gradient formation. [FBrf0211154] Higashi-Kovtun et al., 2010, J. Neurosci. 30(15): 5253--5268 Importin-beta11 regulates synaptic phosphorylated mothers against decapentaplegic, and thereby influences synaptic development and function at the Drosophila neuromuscular junction. [FBrf0210588] Saja et al., 2010, Neurobiol. Disease 40(1): 135--145 Identifying cellular pathways modulated by Drosophila palmitoyl-protein thioesterase 1 function. [FBrf0211567] Evans et al., 2009, Dev. Biol. 326(1): 36--46 Kekkon5 is an extracellular regulator of BMP signaling. [FBrf0207272] Baines, 2004, J. Neurosci. 24(31): 6904--6911 Synaptic strengthening mediated by bone morphogenetic protein-dependent retrograde signaling in the Drosophila CNS. [FBrf0180422] Miguel-Aliaga et al., 2004, Development 131(23): 5837--5848 Independent roles of the dachshund and eyes absent genes in BMP signaling, axon pathfinding and neuronal specification. [FBrf0180178] Allan et al., 2003, Cell 113(1): 73--86 Specification of neuropeptide cell identity by the integration of retrograde BMP signaling and a combinatorial transcription factor code. [FBrf0158766] Sutherland et al., 2003, Development 130(23): 5705--5716 Stepwise formation of a SMAD activity gradient during dorsal-ventral patterning of the Drosophila embryo. [FBrf0167503] Haerry et al., 1998, Development 125(20): 3977--3987 Synergistic signaling by two BMP ligands through the SAX and TKV receptors controls wing growth and patterning in Drosophila. [FBrf0104902]
Supplementary material	Allan et al., 2003, Cell 113(1): Supplementary material: tables S1 - S5. [FBrf0161463]

Allele Dmel\saxQ263D.Scer\UAS.T:Ivir\HA1

Allele Dmel\sax^{Q263D.Scer\UAS.T:Ivir\HA1}