Allele Dmel\pit¹⁰

General Information
Symbol	Dmel\pit¹⁰	Species	D. melanogaster
Name		FlyBase ID	FBal0092554
Feature type	allele	Associated gene	Dmel\pit

Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Zaffran et al., 1998)
Mutagen	P-element activity (Zaffran et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{lacW}B1-93F (Zaffran et al., 1998)
Nature of the lesion	Statement Reference Deletion of 3.5kb of genomic DNA starting from the site of insertion of P{lacW}B1-93F and extending into the pit transcription unit. Part of the P{lacW}B1-93F insertion, including the Ecol\lacZ gene is still present. The proximal breakpoint is in the second exon of pit. (Zaffran et al., 1998)
Caused by insertion	P{?lacW}pit¹⁰ (Zaffran et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Zaffran et al., 1998)
Phenotype Manifest In
	adult midgut precursor cell \| larval stage (Zaffran et al., 1998) embryonic/larval midgut \| embryonic stage (Zaffran et al., 1998) embryonic/larval salivary gland \| embryonic stage (Zaffran et al., 1998) imaginal precursor \| larval stage (Zaffran et al., 1998)
Detailed Description
	Statement Reference Homozygous animals show growth defects; larvae hatch normally and initially show healthy behaviour, although with a constant delay in development compared to wild-type. The larvae are arrested at the first larval instar stage, although they can survive for longer than 7 days. The adult precursor cells and imaginal cells do not proliferate in homozygous larvae. The midgut epithelial cells of 5 day old larvae are smaller than in wild-type third instar larvae of the same age. The level of polyploidy in their nuclei is lower than wild-type. The number of adult midgut precursor cells is smaller in these larvae than in wild-type larvae, corresponding approximately to the number in wild-type first instar rather than third instar larvae. Salivary glands are smaller than in wild-type larvae and do not incorporate BrdU, indicating a failure in DNA replication. Homozygous pit¹⁰ larvae carrying pit^mod.PZ can survive to the third larval instar, although they have a delayed developmental schedule. Only a few of these larvae pupariate and adults are not produced. pit⁵/pit¹⁰ larvae develop normally, but with a delay. Although they pupariate, they never give rise to adults. Homozygous clones in the thorax are small and are only seen when induced late in development (at the end of the third larval instar stage). Homozygous clones in the wing imaginal discs are very small and composed of only a few cells. (Zaffran et al., 1998)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	pit¹⁰ is partially rescued by pit^mod.PZ (Zaffran et al., 1998)
Not rescued by	pit¹⁰ is not rescued by pit^Scer\UAS.cZa/Scer\GAL4^da.G32 (Zaffran et al., 1998) pit¹⁰ is not rescued by pit^Scer\UAS.cZa/Scer\GAL4^e22c (Zaffran et al., 1998) pit¹⁰ is not rescued by pit^Scer\UAS.cZa/Scer\GAL4^hs.PB (Zaffran et al., 1998) pit¹⁰ is not rescued by Scer\GAL4^how-24B/Scer\GAL4^how-24B/pit^Scer\UAS.cZa (Zaffran et al., 1998)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Somatic clonal analysis indicates that pit autonomously affects cell growth. (Zaffran et al., 1998)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pit¹⁰
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Zaffran et al., 1998, Development 125(18): 3571--3584 A Drosophila RNA helicase gene, pitchoune, is required for cell growth and proliferation and is a potential target of d-Myc. [FBrf0104797]

Allele Dmel\pit10

Allele Dmel\pit¹⁰