FB2025_01 , released February 20, 2025
Allele: Dmel\okrAA
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General Information
Symbol
Dmel\okrAA
Species
D. melanogaster
Name
FlyBase ID
FBal0092592
Feature type
allele
Associated gene
Dmel\okr
Associated Insertion(s)
Carried in Construct
Also Known As
okraAA, rad54AA
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Ghabrial et al., 1998)
Mutagen
ethyl methanesulfonate
(Ghabrial et al., 1998)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q9term.

(Ghabrial et al., 1998)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:2,992,628..2,992,628 [-]
Nucleotide change:

C2992628T

Amino acid change:

Q9term | okr-PA

Reported amino acid change:

Q9term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ghabrial et al., 1998)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      okrAA/okrRU mutant females contain an increased number of meiotically-induced double strand breaks (DSBs) compared to controls.

      Many okrAA/okrRU mutant dorsal appendages are abnormal compared to controls. Instead of spherical shaped karyosomes as is seen in wild-type stage 6 egg chambers, the karyosomes of okrAA/okrRU oocytes are fragmented.

      (Lake et al., 2011)

      okrAA/okrRU larvae are highly sensitive to treatment with X-rays or with methyl methanesulfonate.

      okrAA/okrRU mutants show significant decreases in HR-h (homologous recombination using the homologous chromosome) repair compared to controls in a "Repair Reporter 3" assay when the HR-h pathway is available. There is a significant increase in single-strand annealing and in flanking deletions in the mutant flies compared to controls under these conditions.

      (Klovstad et al., 2008)

      Approximately 51% of eggs laid by okrAA homozygous mutant mothers are abnormal, with ventralized eggshells exhibiting partially or completely fused appendages or lack appendages altogether.

      (Abdu et al., 2007)

      okrAA/okrRU females produce eggs with ventralised eggshells.

      (Barbosa et al., 2007)

      Hemizygotes are viable. Eggs derived from fs(1)K10unspecified; okrAA/Df(2L)JS17 females show a broad spectrum of phenotypes ranging from completely dorsalised to completely ventralised. Hemizygous larvae are sensitive to methyl methanesulfonate (MMS) compared to wild-type larvae, showing a significant reduction in survival after being fed with a 0.08% solution of MMS. Recombination frequency is 50% of normal levels in okrAA/okrAO females.

      (Ghabrial et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      okrAA/okrRU has female sterile phenotype, suppressible by vilya826

      (Lake et al., 2015)

      okrAA/okrRU has abnormal meiotic cell cycle phenotype, suppressible by tremf05981

      (Lake et al., 2011)

      okrAA/okrRU has abnormal meiotic cell cycle phenotype, suppressible by tremF9

      (Lake et al., 2011)

      okrAA/okrRU, p53unspecified has female sterile phenotype, suppressible by mei-W681

      (Lu et al., 2010)
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      okrAA/okrRU is an enhancer of visible | maternal effect phenotype of p535A-1-4

      (Chakravarti et al., 2022)

      okrAA/okrRU is an enhancer of visible | maternal effect phenotype of p53B41.5

      (Chakravarti et al., 2022)
      Other
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      okrAA/okrRU has karyosome phenotype, non-enhanceable by p53B41.5

      (Chakravarti et al., 2022)
      Suppressed by
      Statement
      Reference

      okrAA/okrRU has karyosome phenotype, suppressible | partially by p535A-1-4

      (Chakravarti et al., 2022)

      okrAA/okrRU has karyosome phenotype, suppressible | partially by p53A2.3

      (Chakravarti et al., 2022)

      okrAA/okrRU has karyosome phenotype, suppressible | partially by tremF9

      (Lake et al., 2011)

      okrAA/okrRU has karyosome phenotype, suppressible | partially by tremf05981

      (Lake et al., 2011)

      okrAA/okrRU has dorsal appendage phenotype, suppressible | partially by tremF9

      (Lake et al., 2011)

      okrAA/okrRU, p53unspecified has nurse cell phenotype, suppressible by mei-W681

      (Lu et al., 2010)

      okrAA has nucleus phenotype, suppressible by Hus1-like37/Hus1-like[+]

      (Abdu et al., 2007)

      okrAA has egg phenotype, suppressible by Hus1-like37/Hus1-like[+]

      (Abdu et al., 2007)

      okrAA has oocyte phenotype, suppressible by Hus1-like37/Hus1-like[+]

      (Abdu et al., 2007)

      okrAA has dorsal appendage phenotype, suppressible by Hus1-like37/Hus1-like[+]

      (Abdu et al., 2007)

      okrAA has nucleus phenotype, suppressible by Hus1-like37

      (Abdu et al., 2007)

      okrAA has egg phenotype, suppressible by Hus1-like37

      (Abdu et al., 2007)

      okrAA has oocyte phenotype, suppressible by Hus1-like37

      (Abdu et al., 2007)

      okrAA has dorsal appendage phenotype, suppressible by Hus1-like37

      (Abdu et al., 2007)

      okrAA/okrRU has egg chorion phenotype, suppressible by mei-W681/mei-W681

      (Barbosa et al., 2007)

      okrAA/okrRU has egg chorion phenotype, suppressible by lokp6/lokp6

      (Abdu et al., 2002)

      okrAA/okrRU has oocyte nucleus phenotype, suppressible by lokp6/lokp6

      (Abdu et al., 2002)

      okrAA/okrRU has egg chorion | maternal effect phenotype, suppressible | maternal effect by mei-W68unspecified/Df(2R)LL5

      (Ghabrial and Schupbach, 1999)
      NOT suppressed by
      Statement
      Reference

      okrAA/okrRU has karyosome phenotype, non-suppressible by p53B41.5

      (Chakravarti et al., 2022)

      okrAA has karyosome phenotype, non-suppressible by Hus1-like37

      (Abdu et al., 2007)

      okrAA has karyosome phenotype, non-suppressible by Hus1-like37/Hus1-like[+]

      (Abdu et al., 2007)

      okrAA/okrRU has egg chorion phenotype, non-suppressible by mus304D1

      (Abdu et al., 2002)

      okrAA/okrRU has egg chorion phenotype, non-suppressible by p5311-1B-1

      (Abdu et al., 2002)

      okrAA/okrRU has dorsal appendage phenotype, non-suppressible by p5311-1B-1

      (Abdu et al., 2002)

      okrAA/okrRU has dorsal appendage phenotype, non-suppressible by mus304D1

      (Abdu et al., 2002)
      Enhancer of
      Statement
      Reference

      okrAA/okrRU is an enhancer of egg | maternal effect phenotype of p535A-1-4

      (Chakravarti et al., 2022)

      okrAA/okrRU is an enhancer of egg | maternal effect phenotype of p53B41.5

      (Chakravarti et al., 2022)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      tremf05981 suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okrAA/okrRU mutant females.

      tremF9 partially suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okrAA/okrRU mutant females.

      The karyosome defects observed in okrAA/okrRU are almost completely suppressed in the background of tremF9. The dorsal appendage defects are also partially suppressed.

      The karyosome defects observed in okrAA/okrRU are almost completely suppressed in the background of tremf05981.

      (Lake et al., 2011)

      Single-gene mutants show 15 nurse cell nuclei per egg chamber, but p53unspecified, okrAA/okrRU ovaries exhibit a broad distribution, ranging from 9 to 40 nuclei per egg chamber, which is restored to normal in mei-W681, okrAA/okrRU, p53unspecified animals.

      Single-gene mutants are fertile, but p53unspecified, okrAA/okrRU double mutants are sterile. Fertility is restored in mei-W681, okrAA/okrRU, p53unspecified triple mutant females.

      (Lu et al., 2010)

      A heterozygous Hus1-like37 background partially suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers, with only 35% of eggs exhibiting partially or completely fused appendages or lacking appendages altogether, compared to 51% in okrAA mutants.

      A homozygous Hus1-like37 background completely suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers.

      A heterozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

      A homozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

      (Abdu et al., 2007)

      The ventralised eggshell phenotype produced by okrAA/okrRU females is suppressed by mei-W681.

      (Barbosa et al., 2007)

      okrAA/Df(2L)JS17 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38oC, but show a higher frequency of SSA than controls in the same assay at 32oC.

      okrAA/Df(2L)JS17 mutants have a reduced frequency of interhomolog gene conversion (GC) and a corresponding increase in SSA frequencies compared to controls in a P{wIw.FRT}/P{wIw.FRT.yellow} homozygous assay to study DNA double-stranded break repair when assayed at both 32oC and 38oC.

      okrAA/Df(2L)JS17 mutants have an increased SSA frequency compared to controls in a P{wIw.FRT}/P{wIw.FRT.8z} homozygous assay to study DNA double-stranded break repair when assayed at 38oC.

      (Wei and Rong, 2007)

      The eggshell patterning defects of eggs derived from okrRU/okrAA females are suppressed if the females also carry mei-W68unspecified/Df(2R)LL5.

      (Ghabrial and Schupbach, 1999)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      okrWS

      (Ghabrial et al., 1998)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (18)