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General Information
Symbol
Dmel\okrAA
Species
D. melanogaster
Name
FlyBase ID
FBal0092592
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
okraAA, rad54AA
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C2992628T

Amino acid change:

Q9term | okr-PA

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q9term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

okrAA/okrRU mutant females contain an increased number of meiotically-induced double strand breaks (DSBs) compared to controls.

Many okrAA/okrRU mutant dorsal appendages are abnormal compared to controls. Instead of spherical shaped karyosomes as is seen in wild-type stage 6 egg chambers, the karyosomes of okrAA/okrRU oocytes are fragmented.

okrAA/okrRU larvae are highly sensitive to treatment with X-rays or with methyl methanesulfonate.

okrAA/okrRU mutants show significant decreases in HR-h (homologous recombination using the homologous chromosome) repair compared to controls in a "Repair Reporter 3" assay when the HR-h pathway is available. There is a significant increase in single-strand annealing and in flanking deletions in the mutant flies compared to controls under these conditions.

Approximately 51% of eggs laid by okrAA homozygous mutant mothers are abnormal, with ventralized eggshells exhibiting partially or completely fused appendages or lack appendages altogether.

okrAA/okrRU females produce eggs with ventralised eggshells.

Hemizygotes are viable. Eggs derived from fs(1)K10unspecified; okrAA/Df(2L)JS17 females show a broad spectrum of phenotypes ranging from completely dorsalised to completely ventralised. Hemizygous larvae are sensitive to methyl methanesulfonate (MMS) compared to wild-type larvae, showing a significant reduction in survival after being fed with a 0.08% solution of MMS. Recombination frequency is 50% of normal levels in okrAA/okrAO females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference

okrAA/okrRU has karyosome phenotype, suppressible | partially by tremF9

okrAA/okrRU has dorsal appendage phenotype, suppressible | partially by tremF9

okrAA/okrRU has karyosome phenotype, suppressible | partially by tremf05981

okrAA/okrRU, p53unspecified has nurse cell phenotype, suppressible by mei-W681

okrAA has oocyte phenotype, suppressible | partially by Hus1-like37/Hus1-like[+]

okrAA has dorsal appendage phenotype, suppressible | partially by Hus1-like37/Hus1-like[+]

okrAA has nucleus phenotype, suppressible by Hus1-like37

okrAA has egg phenotype, suppressible by Hus1-like37

okrAA has oocyte phenotype, suppressible by Hus1-like37

okrAA has dorsal appendage phenotype, suppressible by Hus1-like37

okrAA has nucleus phenotype, suppressible | partially by Hus1-like37/Hus1-like[+]

okrAA has egg phenotype, suppressible | partially by Hus1-like37/Hus1-like[+]

okrAA/okrRU has chorion phenotype, suppressible by mei-W681/mei-W681

okrAA/okrRU has chorion phenotype, suppressible by lokp6/lokp6/lokp6/lokp6

NOT suppressed by
Statement
Reference

okrAA has karyosome phenotype, non-suppressible by Hus1-like37/Hus1-like[+]

okrAA has karyosome phenotype, non-suppressible by Hus1-like37

okrAA/okrRU has chorion phenotype, non-suppressible by mus304D1

okrAA/okrRU has dorsal appendage phenotype, non-suppressible by mus304D1

okrAA/okrRU has chorion phenotype, non-suppressible by p5311-1B-1

okrAA/okrRU has dorsal appendage phenotype, non-suppressible by p5311-1B-1

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

tremf05981 suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okrAA/okrRU mutant females.

tremF9 partially suppresses the accumulation of meiotically-induced double stranded breaks seen in region 3 of the germarium in okrAA/okrRU mutant females.

The karyosome defects observed in okrAA/okrRU are almost completely suppressed in the background of tremF9. The dorsal appendage defects are also partially suppressed.

The karyosome defects observed in okrAA/okrRU are almost completely suppressed in the background of tremf05981.

Single-gene mutants show 15 nurse cell nuclei per egg chamber, but p53unspecified, okrAA/okrRU ovaries exhibit a broad distribution, ranging from 9 to 40 nuclei per egg chamber, which is restored to normal in mei-W681, okrAA/okrRU, p53unspecified animals.

Single-gene mutants are fertile, but p53unspecified, okrAA/okrRU double mutants are sterile. Fertility is restored in mei-W681, okrAA/okrRU, p53unspecified triple mutant females.

A heterozygous Hus1-like37 background partially suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers, with only 35% of eggs exhibiting partially or completely fused appendages or lacking appendages altogether, compared to 51% in okrAA mutants.

A homozygous Hus1-like37 background completely suppresses the abnormal egg phenotype for eggs laid by okrAA homozygous mutant mothers.

A heterozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

A homozygous Hus1-like37 background has no effect on the abnormal karyosome phenotype associated with okrAA homozygous mutants.

The ventralised eggshell phenotype produced by okrAA/okrRU females is suppressed by mei-W681.

okrAA/Df(2L)JS17 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38oC, but show a higher frequency of SSA than controls in the same assay at 32oC.

okrAA/Df(2L)JS17 mutants have a reduced frequency of interhomolog gene conversion (GC) and a corresponding increase in SSA frequencies compared to controls in a P{wIw.FRT}/P{wIw.FRT.yellow} homozygous assay to study DNA double-stranded break repair when assayed at both 32oC and 38oC.

okrAA/Df(2L)JS17 mutants have an increased SSA frequency compared to controls in a P{wIw.FRT}/P{wIw.FRT.8z} homozygous assay to study DNA double-stranded break repair when assayed at 38oC.

The eggshell patterning defects of eggs derived from okrRU/okrAA females are suppressed if the females also carry mei-W68unspecified/Df(2R)LL5.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (16)