Allele Dmel\hep^Scer\UAS.cBa

General Information
Symbol	Dmel\hep^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Boutros	FlyBase ID	FBal0092957
Feature type	allele	Associated gene	Dmel\hep

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Boutros et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of hep cDNA is governed by Scer\UAS regulatory sequences. (Boutros et al., 1998)
Carried in construct	P{UAS-hep.B} (Wang et al., 2005, Lee et al., 2003, Shulman and Feany, 2003, Weber et al., 2000, Kumar and Moses, 2001, McEwen et al., 2000, Zeitlinger and Bohmann, 1999, Boutros et al., 1998, Wang et al., 2003, Kumar and Moses, 2001, Cha et al., 2003, Hull-Thompson et al., 2009, Srahna et al., 2006, Uhlirova and Bohmann, 2006, Rallis et al., 2010, Rousset et al., 2010, Gettings et al., 2010, Mathew et al., 2011, Biteau et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant, with Scer\GAL4^arm.PS (Wang et al., 2003) chemical resistant \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Wang et al., 2003) long lived, with Scer\GAL4^elav-C155 (Wang et al., 2003) planar polarity defective, with Scer\GAL4^hs.2sev (Weber et al., 2000) short lived \| heat sensitive, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Biteau et al., 2010) stress response defective, with Scer\GAL4^ppl.PP (Hull-Thompson et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Zeitlinger and Bohmann, 1999)
Phenotype Manifest In
	adult gut \| heat sensitive, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Biteau et al., 2010) adult thorax & macrochaeta, with Scer\GAL4^pnr-MD237 (Zeitlinger and Bohmann, 1999) adult thorax \| dorsal, with Scer\GAL4^pnr-MD237 (Zeitlinger and Bohmann, 1999) embryonic/first instar larval cuticle, with Scer\GAL4^69B (Rousset et al., 2010) embryonic leading edge cell, with Scer\GAL4^ptc-559.1 (Gettings et al., 2010) eye, with Scer\GAL4^hs.2sev (Weber et al., 2000) genital disc, with Scer\GAL4^Abd-B-LDN (Rousset et al., 2010) genital disc, with Scer\GAL4^ptc-559.1 (Rousset et al., 2010) ommatidium, with Scer\GAL4^hs.2sev (Weber et al., 2000) scutellum, with Scer\GAL4^pnr-MD237 (Zeitlinger and Bohmann, 1999) ventral furrow, with Scer\GAL4^twi.PG (Mathew et al., 2011)
Detailed Description
	Statement Reference Expression of hep[Scer\UAS.cBa] under the control of Scer\GAL4[twi.PG] results in delayed or defective ventral furrow formation in a proportion of embryos. (Mathew et al., 2011) Transient overexpression of hep[Scer\UAS.cBa] in adults under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B] induces increased proliferation of intestinal cells compared with controls. Transient overexpression of hep[Scer\UAS.cBa] in adults under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B] results in significant lifespan shortening compared with wild-type. (Biteau et al., 2010) Expression of hep[Scer\UAS.cBa] under the control of Scer\GAL4[ptc-559.1] results in a dramatic increase in the number of intercalated cells and the formation of ectopic mixer cells at the segment boundaries during dorsal closure (in the abdominal segments of wild-type embryos at the end of dorsal closure, a mixer cell moves across the segment boundary from the anterior compartment to the posterior compartment and two cells from the ventral ectoderm intercalate into the leading edge, posterior to the mixer cell). (Gettings et al., 2010) Ectopic expression of hep[Scer\UAS.cBa] driven by Scer\GAL4[69B] in embryos leads to cuticles with openings in the anterior part and puckering in the dorsal part. Ectopic expression of hep[Scer\UAS.cBa] driven by either Scer\GAL4[Abd-B-LDN] or Scer\GAL4[ptc-559.1] leads to male genital disc effects such as incomplete rotation and separation of the plate from the abdomen. (Rousset et al., 2010) Animals expressing hep[Scer\UAS.cBa] under the control of Scer\GAL4[ppl.PP] show increased tolerance to paraquat compared to controls. (Hull-Thompson et al., 2009) hep^Scer\UAS.cBa Scer\GAL4^GMR.PF adults have normal eyes. (Wang et al., 2005) Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF does not affect eye development. (Cha et al., 2003) Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF has no effect on eye morphology. (Shulman and Feany, 2003) Flies expressing hep^Scer\UAS.cBa, under the control of Scer\GAL4^arm.PS, show a decreased sensitivity to paraquat compared to wild-type flies. This decreased sensitivity is also seen when hep^Scer\UAS.cBa is expressed under the control of the neuron-specific driver Scer\GAL4^{elav.Switch.PO}, but only in the presence of RU486. However, expression of hep^Scer\UAS.cBa under the control of the muscle-specific driver Scer\GAL4^{Mhc.Switch.PO} does not affect tolerance to paraquat. Overexpression of hep^Scer\UAS.cBa, driven by Scer\GAL4^elav-C155, increases the lifespan of flies. (Wang et al., 2003) When expression is driven by Scer\GAL4^hs.2sev, flies have rough eyes showing polarity phenotypes. Ommatidia are incorrectly rotated and sometimes exhibit the wrong chiral form. (Weber et al., 2000) Adults in which hep^Scer\UAS.cBa is expressed under the control of Scer\GAL4^pnr-MD237 have a severely reduced scutellum, and there is a cleft in the tissue at the dorsal midline. The bristles are oriented in a different direction from normal, are loosely spaced and sometimes have kinks. (Zeitlinger and Bohmann, 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^pnr-MD237, hep^Scer\UAS.cBa has visible phenotype, enhanceable by puc^E69 (Zeitlinger and Bohmann, 1999)
Enhancer of
Statement Reference hep^Scer\UAS.cBa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI is an enhancer of tumorigenic \| somatic clone phenotype of Ras85D^V12.Scer\UAS, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Uhlirova and Bohmann, 2006) hep^Scer\UAS.cBa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003) hep^Scer\UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, by^Scer\UAS.cLa (Lee et al., 2003)
Suppressor of
Statement Reference hep^Scer\UAS.cBa/Scer\GAL4^ato.3.6 is a suppressor of neuroanatomy defective phenotype of dsh¹ (Srahna et al., 2006) hep^Scer\UAS.cBa/Scer\GAL4^ey-OK107 is a suppressor of neuroanatomy defective \| somatic clone phenotype of Mkk4^e01485 (Rallis et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^pnr-MD237, hep^Scer\UAS.cBa has scutellum phenotype, enhanceable by puc^E69 (Zeitlinger and Bohmann, 1999)
Suppressed by
Statement Reference Scer\GAL4^twi.PG, hep^Scer\UAS.cBa has ventral furrow phenotype, suppressible \| germline clone by bsk^unspecified (Mathew et al., 2011)
Enhancer of
Statement Reference hep^Scer\UAS.cBa, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, foxo^Scer\UAS.cPa (Wang et al., 2005) hep^Scer\UAS.cBa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003) hep^Scer\UAS.cBa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Shulman and Feany, 2003) hep^Scer\UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, by^Scer\UAS.cLa (Lee et al., 2003)
Suppressor of
Statement Reference hep^Scer\UAS.cBa/Scer\GAL4^{arm.T:Hsim\VP16} is a suppressor \| partially of embryonic epidermis \| dorsal phenotype of arm³ (McEwen et al., 2000) hep^Scer\UAS.cBa/Scer\GAL4^{arm.T:Hsim\VP16} is a suppressor \| partially of embryonic epidermis \| ventral phenotype of arm³ (McEwen et al., 2000) hep^Scer\UAS.cBa/Scer\GAL4^ato.3.6 is a suppressor of axon & dorsal cluster neuron phenotype of dsh¹ (Srahna et al., 2006) hep^Scer\UAS.cBa/Scer\GAL4^ey-OK107 is a suppressor of axon \| somatic clone phenotype of Mkk4^e01485 (Rallis et al., 2010) hep^Scer\UAS.cBa/Scer\GAL4^ey-OK107 is a suppressor of Kenyon cell \| somatic clone phenotype of Mkk4^e01485 (Rallis et al., 2010)
Other
Statement Reference Ras85D^V12.Scer\UAS, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hep^Scer\UAS.cBa has embryonic/larval brain \| somatic clone phenotype (Uhlirova and Bohmann, 2006) Ras85D^V12.Scer\UAS, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, hep^Scer\UAS.cBa has ventral nerve cord \| somatic clone phenotype (Uhlirova and Bohmann, 2006)
Additional Comments
Genetic Interactions
Statement Reference The defects in ventral furrow formation seen in embryos expressing hep[Scer\UAS.cBa] under the control of Scer\GAL4[twi.PG] are completely suppressed in embryos derived from bsk[unspecified] female germline clones. (Mathew et al., 2011) The axon overextension phenotype of Mkk4[e01485] neuroblast clones in mushroom bodies is suppressed by expression of hep[Scer\UAS.cBa] using Scer\GAL4[ey-OK107]. (Rallis et al., 2010) The increased tolerance of animals expressing hep[Scer\UAS.cBa] under the control of Scer\GAL4[ppl.PP] to paraquat is abolished by NLaz[NW5]/NLaz[NW5], with the double mutant flies showing the same sensitivity to paraquat as NLaz[NW5]/NLaz[NW5] single mutants. (Hull-Thompson et al., 2009) Expression of hep^Scer\UAS.cBa, under the control of Scer\GAL4^ato.3.6, in a dsh¹ background results in a large increase of dorsal cluster neuron axons crossing the optic chiasm, indicating a complete dominance of the hep gain of function phenotype. (Srahna et al., 2006) Coexpression of hep^Scer\UAS.cBa and Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI results in eye/antennal imaginal disc clones results in the invasion of the disc cells into the ventral nerve cord and regions of the brain. (Uhlirova and Bohmann, 2006) The small, deformed eye phenotype of foxo^Scer\UAS.cPa; Scer\GAL4^GMR.PF adults is enhanced by the presence of hep^Scer\UAS.cBa. (Wang et al., 2005) When hep^Scer\UAS.cBa is coexpressed with one copy of Traf1^EP578, driven by Scer\GAL4^GMR.PF, the number of ommatidia and the size of the compound eye are reduced to similar levels seen when two copies of Traf1^EP578 are expressed. Coexpression of Traf2^EP1516 with either hep^Scer\UAS.cBa, under the control of Scer\GAL4^GMR.PF, does not affect the development of eyes. (Cha et al., 2003) The phenotype caused when hep^Scer\UAS.cBa is expressed under the control of Scer\GAL4^pnr-MD237is enhanced by a single copy of puc^E69; the scutellum is completely missing. (Zeitlinger and Bohmann, 1999)
Xenogenetic Interactions
Statement Reference The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of hep^Scer\UAS.cBa; eye surface roughness is increased, eye size is decreased and necrotic black patches are formed in the eye. (Shulman and Feany, 2003)
Complementation & Rescue Data
Rescues	hep^Scer\UAS.cBa/Scer\GAL4^ey-OK107 rescues hep^r39 (Rallis et al., 2010)
Comments	Expression of hep[Scer\UAS.cBa] with Scer\GAL4[ey-OK107] rescues the axonal phenotypes seen in hep[r39] mushroom body clones. (Rallis et al., 2010)
Stocks ( 2 )
Bloomington	9308 w^; P{UAS-hep.B}2 9307 P{UAS-hep.B}1, w^/FM6, w^*
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	hep^Scer\UAS.cBa hep^UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Boutros (Boutros et al., 1998)
Secondary FlyBase IDs

References ( 19 )
Research paper	Mathew et al., 2011, Mol. Cell. Biol. 31(24): 4978--4993 Role for traf4 in polarizing adherens junctions as a prerequisite for efficient cell shape changes. [FBrf0216703] Biteau et al., 2010, PLoS Genet. 6(10): e1001159 Lifespan extension by preserving proliferative homeostasis in Drosophila. [FBrf0212112] Gettings et al., 2010, PLoS Biol. 8(6): e1000390 JNK Signalling Controls Remodelling of the Segment Boundary through Cell Reprogramming during Drosophila Morphogenesis. [FBrf0211014] Rallis et al., 2010, Dev. Biol. 339(1): 65--77 Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability. [FBrf0209975] Rousset et al., 2010, Development 137(13): 2177--2186 The Drosophila serine protease homologue Scarface regulates JNK signalling in a negative-feedback loop during epithelial morphogenesis. [FBrf0211045] Hull-Thompson et al., 2009, PLoS Genet. 5(4): e1000460 Control of metabolic homeostasis by stress signaling is mediated by the lipocalin NLaz. [FBrf0207766] Srahna et al., 2006, PLoS Biol. 4(11): e348 A signaling network for patterning of neuronal connectivity in the Drosophila brain. [FBrf0192599] Uhlirova and Bohmann, 2006, EMBO J. 25(22): 5294--5304 JNK- and Fos-regulated Mmp1 expression cooperates with Ras to induce invasive tumors in Drosophila. [FBrf0194142] Wang et al., 2005, Cell 121(1): 115--125 JNK extends life span and limits growth by antagonizing cellular and organism-wide responses to insulin signaling. [FBrf0187242] Cha et al., 2003, Mol. Cell. Biol. 23(22): 7982--7991 Discrete functions of TRAF1 and TRAF2 in Drosophila melanogaster mediated by c-Jun N-terminal kinase and NF-kappaB-dependent signaling pathways. [FBrf0167884] Lee et al., 2003, Development 130(17): 4001--4010 blistery encodes Drosophila tensin protein and interacts with integrin and the JNK signaling pathway during wing development. [FBrf0160724] Shulman and Feany, 2003, Genetics 165(3): 1233--1242 Genetic modifiers of tauopathy in Drosophila. [FBrf0167629] Wang et al., 2003, Dev. Cell 5(5): 811--816 JNK signaling confers tolerance to oxidative stress and extends lifespan in Drosophila. [FBrf0167481] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] McEwen et al., 2000, Development 127(16): 3607--3617 The canonical Wg and JNK signaling cascades collaborate to promote both dorsal closure and ventral patterning. [FBrf0128573] Weber et al., 2000, Development 127(16): 3619--3629 Jun mediates Frizzled-induced R3/R4 cell fate distinction and planar polarity determination in the Drosophila eye. [FBrf0128678] Zeitlinger and Bohmann, 1999, Development 126(17): 3947--3956 Thorax closure in Drosophila: involvement of fos and the JNK pathway. [FBrf0111528] Boutros et al., 1998, Cell 94(1): 109--118 Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. [FBrf0103240]

Allele Dmel\hepScer\UAS.cBa

Allele Dmel\hep^Scer\UAS.cBa