|Name||Saccharomyces cerevisiae UAS construct a of Boutros||FlyBase ID||FBal0092957|
|Feature type||allele||Associated gene||Dmel\hep|
|Mutagen||in vitro construct - regulatory fusion|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Wang et al., 2005, Lee et al., 2003, Shulman and Feany, 2003, Weber et al., 2000, Kumar and Moses, 2001, McEwen et al., 2000, Zeitlinger and Bohmann, 1999, Boutros et al., 1998, Wang et al., 2003, Kumar and Moses, 2001, Cha et al., 2003, Hull-Thompson et al., 2009, Srahna et al., 2006, Uhlirova and Bohmann, 2006, Rallis et al., 2010, Rousset et al., 2010, Gettings et al., 2010, Mathew et al., 2011, Biteau et al., 2010)
|Phenotype Manifest In|
Expression of hep[Scer\UAS.cBa] under the control of Scer\GAL4[twi.PG] results in delayed or defective ventral furrow formation in a proportion of embryos.
Transient overexpression of hep[Scer\UAS.cBa] in adults under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B] induces increased proliferation of intestinal cells compared with controls. Transient overexpression of hep[Scer\UAS.cBa] in adults under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B] results in significant lifespan shortening compared with wild-type.
Expression of hep[Scer\UAS.cBa] under the control of Scer\GAL4[ptc-559.1] results in a dramatic increase in the number of intercalated cells and the formation of ectopic mixer cells at the segment boundaries during dorsal closure (in the abdominal segments of wild-type embryos at the end of dorsal closure, a mixer cell moves across the segment boundary from the anterior compartment to the posterior compartment and two cells from the ventral ectoderm intercalate into the leading edge, posterior to the mixer cell).
Ectopic expression of hep[Scer\UAS.cBa] driven by Scer\GAL4[69B] in embryos leads to cuticles with openings in the anterior part and puckering in the dorsal part. Ectopic expression of hep[Scer\UAS.cBa] driven by either Scer\GAL4[Abd-B-LDN] or Scer\GAL4[ptc-559.1] leads to male genital disc effects such as incomplete rotation and separation of the plate from the abdomen.
Animals expressing hep[Scer\UAS.cBa] under the control of Scer\GAL4[ppl.PP] show increased tolerance to paraquat compared to controls.
Flies expressing hepScer\UAS.cBa, under the control of Scer\GAL4arm.PS, show a decreased sensitivity to paraquat compared to wild-type flies. This decreased sensitivity is also seen when hepScer\UAS.cBa is expressed under the control of the neuron-specific driver Scer\GAL4elav.Switch.PO, but only in the presence of RU486. However, expression of hepScer\UAS.cBa under the control of the muscle-specific driver Scer\GAL4Mhc.Switch.PO does not affect tolerance to paraquat. Overexpression of hepScer\UAS.cBa, driven by Scer\GAL4elav-C155, increases the lifespan of flies.
When expression is driven by Scer\GAL4hs.2sev, flies have rough eyes showing polarity phenotypes. Ommatidia are incorrectly rotated and sometimes exhibit the wrong chiral form.
Adults in which hepScer\UAS.cBa is expressed under the control of Scer\GAL4pnr-MD237 have a severely reduced scutellum, and there is a cleft in the tissue at the dorsal midline. The bristles are oriented in a different direction from normal, are loosely spaced and sometimes have kinks.
hepScer\UAS.cBa, Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI is an enhancer of tumorigenic | somatic clone phenotype of Ras85DV12.Scer\UAS, Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI
hepScer\UAS.cBa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is an enhancer of visible phenotype of Hsap\MAPTV337M.Scer\UAS, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
|Phenotype Manifest In|
hepScer\UAS.cBa, Scer\GAL4GMR.PF, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, foxoScer\UAS.cPa
hepScer\UAS.cBa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is an enhancer of eye phenotype of Hsap\MAPTV337M.Scer\UAS, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
hepScer\UAS.cBa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPTV337M.Scer\UAS, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
hepScer\UAS.cBa/Scer\GAL4arm.T:Hsim\VP16 is a suppressor | partially of embryonic epidermis | dorsal phenotype of arm3
hepScer\UAS.cBa/Scer\GAL4arm.T:Hsim\VP16 is a suppressor | partially of embryonic epidermis | ventral phenotype of arm3
Ras85DV12.Scer\UAS, Scer\GAL4Act5C.PI/Scer\GAL4Act5C.PI, hepScer\UAS.cBa has embryonic/larval brain | somatic clone phenotype
The defects in ventral furrow formation seen in embryos expressing hep[Scer\UAS.cBa] under the control of Scer\GAL4[twi.PG] are completely suppressed in embryos derived from bsk[unspecified] female germline clones.
The axon overextension phenotype of Mkk4[e01485] neuroblast clones in mushroom bodies is suppressed by expression of hep[Scer\UAS.cBa] using Scer\GAL4[ey-OK107].
The increased tolerance of animals expressing hep[Scer\UAS.cBa] under the control of Scer\GAL4[ppl.PP] to paraquat is abolished by NLaz[NW5]/NLaz[NW5], with the double mutant flies showing the same sensitivity to paraquat as NLaz[NW5]/NLaz[NW5] single mutants.
Expression of hepScer\UAS.cBa, under the control of Scer\GAL4ato.3.6, in a dsh1 background results in a large increase of dorsal cluster neuron axons crossing the optic chiasm, indicating a complete dominance of the hep gain of function phenotype.
Coexpression of hepScer\UAS.cBa and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.PI results in eye/antennal imaginal disc clones results in the invasion of the disc cells into the ventral nerve cord and regions of the brain.
The small, deformed eye phenotype of foxoScer\UAS.cPa; Scer\GAL4GMR.PF adults is enhanced by the presence of hepScer\UAS.cBa.
When hepScer\UAS.cBa is coexpressed with one copy of Traf1EP578, driven by Scer\GAL4GMR.PF, the number of ommatidia and the size of the compound eye are reduced to similar levels seen when two copies of Traf1EP578 are expressed. Coexpression of Traf2EP1516 with either hepScer\UAS.cBa, under the control of Scer\GAL4GMR.PF, does not affect the development of eyes.
|Complementation & Rescue Data|
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Boutros
|Secondary FlyBase IDs|
|References ( 19 )|