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General Information
Symbol
Dmel\hepUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Boutros
FlyBase ID
FBal0092957
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hep, UAS-hepwt, UASHep, UAS::hep
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of hep cDNA is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

adult thorax & macrochaeta, with Scer\GAL4pnr-MD237

Detailed Description
Statement
Reference

Expression of hepUAS.cBa under the control of Scer\GAL4esg.PU, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, results in an increase in progenitor cells and stem cell division, as well as distortion of the epithelial architecture in the gut, compared to controls; adult-stage expression under the control of Scer\GAL4NP0001 results in reduced numbers of enterocytes in the gut, but a significant increase in stem cell division and increased numbers of pre-enterocytes, compared to controls.

Expressing hepUAS.cBa under the control of Scer\GAL4ptc.PU induces some cells at the wing disc A/P boundary to delaminate and migrate to the posterior compartment, as well as cell death at the boundary (acridine orange staining). Expression under the control of Scer\GAL4GMR.PU also induces cell death, but not cell invasion, in the eye disc.

The expression of hepScer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 does not lead to obvious size defects in third instar larval wing discs, as compared to controls.

Expression of hepScer\UAS.cBa under the control of Scer\GAL4esg-NP5130 dramatically increases the number of mitotic cells in the adult gut.

Expression of hepScer\UAS.cBa under the control of Scer\GAL4esg-NP5130 (using tub-gal80[ts] to limit the time of expression) increases cell proliferation in the adult gut.

Expression of hepScer\UAS.cBa driven by Scer\GAL4pnr-MD237 triggers cell death in the thorax and produces a small-scutellum phenotype.

Expression of hepScer\UAS.cBa along the anterior-posterior compartment boundary driven by Scer\GAL4ptc-559.1 induces elevated cell death in third instar wing discs relative to wild-type and results in the loss of the anterior crossvein.

Expression of hepScer\UAS.cBa under the control of Scer\GAL4twi.PG results in delayed or defective ventral furrow formation in a proportion of embryos.

Transient overexpression of hepScer\UAS.cBa in adults under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B induces increased proliferation of intestinal cells compared with controls.

Transient overexpression of hepScer\UAS.cBa in adults under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in significant lifespan shortening compared with wild-type.

Expression of hepScer\UAS.cBa under the control of Scer\GAL4ptc-559.1 results in a dramatic increase in the number of intercalated cells and the formation of ectopic mixer cells at the segment boundaries during dorsal closure (in the abdominal segments of wild-type embryos at the end of dorsal closure, a mixer cell moves across the segment boundary from the anterior compartment to the posterior compartment and two cells from the ventral ectoderm intercalate into the leading edge, posterior to the mixer cell).

Ectopic expression of hepScer\UAS.cBa driven by Scer\GAL469B in embryos leads to cuticles with openings in the anterior part and puckering in the dorsal part.

Ectopic expression of hepScer\UAS.cBa driven by either Scer\GAL4Abd-B-LDN or Scer\GAL4ptc-559.1 leads to male genital disc effects such as incomplete rotation and separation of the plate from the abdomen.

Animals expressing hepScer\UAS.cBa under the control of Scer\GAL4ppl.PP show increased tolerance to paraquat compared to controls.

Expression of hepScer\UAS.cBa under the control of Scer\GAL4GMR.PF does not affect eye development.

Expression of hepScer\UAS.cBa under the control of Scer\GAL4GMR.PF has no effect on eye morphology.

Flies expressing hepScer\UAS.cBa, under the control of Scer\GAL4arm.PS, show a decreased sensitivity to paraquat compared to wild-type flies. This decreased sensitivity is also seen when hepScer\UAS.cBa is expressed under the control of the neuron-specific driver Scer\GAL4elav.Switch.PO, but only in the presence of RU486. However, expression of hepScer\UAS.cBa under the control of the muscle-specific driver Scer\GAL4Mhc.Switch.PO does not affect tolerance to paraquat. Overexpression of hepScer\UAS.cBa, driven by Scer\GAL4elav-C155, increases the lifespan of flies.

When expression is driven by Scer\GAL4hs.2sev, flies have rough eyes showing polarity phenotypes. Ommatidia are incorrectly rotated and sometimes exhibit the wrong chiral form.

Adults in which hepScer\UAS.cBa is expressed under the control of Scer\GAL4pnr-MD237 have a severely reduced scutellum, and there is a cleft in the tissue at the dorsal midline. The bristles are oriented in a different direction from normal, are loosely spaced and sometimes have kinks.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4pnr-MD237, hepUAS.cBa has visible phenotype, suppressible | partially by wg[+]/wgl-8

Scer\GAL4pnr-MD237, hepUAS.cBa has visible phenotype, suppressible | partially by dsh3/dsh[+]

Scer\GAL4pnr-MD237, hepUAS.cBa has visible phenotype, suppressible | partially by arm2/arm[+]

NOT suppressed by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4pnr-MD237, hepUAS.cBa has scutellum phenotype, suppressible | partially by wg[+]/wgl-8

Scer\GAL4pnr-MD237, hepUAS.cBa has scutellum phenotype, suppressible | partially by dsh3/dsh[+]

Scer\GAL4pnr-MD237, hepUAS.cBa has scutellum phenotype, suppressible | partially by arm2/arm[+]

NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of hepScer\UAS.cBa and aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4Bx-MS1096 leads to moderate overgrowth and cell polarity defects in third instar larval wing discs, as compared to controls.

The increased cell proliferation observed upon expression of hepScer\UAS.cBa under the control of Scer\GAL4esg-NP5130 in the adult gut is suppressed by co-expression of either Sox21aJF02191 or kaydsRNA.Scer\UAS.

The increased cell proliferation in the adult gut expressing hepScer\UAS.cBa under the control of Scer\GAL4esg-NP5130 (using tub-gal80[ts] to limit the time of expression) is suppressed by co-expression of PEKGD5584.

Expression of hepScer\UAS.cBa under the control of Scer\GAL4elav.PU fully rescues the reduced oxidative stress resistance and shortened lifespan seen in ben1 mutant males.

The reduced wing phenotype caused by expression of DLPEY09290 under the control of Scer\GAL4Bx-MS1096 is strongly exacerbated by co-expression of hepScer\UAS.cBa.

The defects in ventral furrow formation seen in embryos expressing hepScer\UAS.cBa under the control of Scer\GAL4twi.PG are completely suppressed in embryos derived from bskunspecified female germline clones.

The axon overextension phenotype of Mkk4e01485 neuroblast clones in mushroom bodies is suppressed by expression of hepScer\UAS.cBa using Scer\GAL4ey-OK107.

The increased tolerance of animals expressing hepScer\UAS.cBa under the control of Scer\GAL4ppl.PP to paraquat is abolished by NLazNW5/NLazNW5, with the double mutant flies showing the same sensitivity to paraquat as NLazNW5/NLazNW5 single mutants.

Expression of hepScer\UAS.cBa, under the control of Scer\GAL4ato.3.6, in a dsh1 background results in a large increase of dorsal cluster neuron axons crossing the optic chiasm, indicating a complete dominance of the hep gain of function phenotype.

Coexpression of hepScer\UAS.cBa and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.PI results in eye/antennal imaginal disc clones results in the invasion of the disc cells into the ventral nerve cord and regions of the brain.

The small, deformed eye phenotype of foxoScer\UAS.cPa; Scer\GAL4GMR.PF adults is enhanced by the presence of hepScer\UAS.cBa.

When hepScer\UAS.cBa is coexpressed with one copy of Traf1EP578, driven by Scer\GAL4GMR.PF, the number of ommatidia and the size of the compound eye are reduced to similar levels seen when two copies of Traf1EP578 are expressed.

Coexpression of Traf2EP1516 with either hepScer\UAS.cBa, under the control of Scer\GAL4GMR.PF, does not affect the development of eyes.

The phenotype caused when hepScer\UAS.cBa is expressed under the control of Scer\GAL4pnr-MD237is enhanced by a single copy of pucE69; the scutellum is completely missing.

Xenogenetic Interactions
Statement
Reference

The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by co-expression of hepScer\UAS.cBa; eye surface roughness is increased, eye size is decreased and necrotic black patches are formed in the eye.

Complementation and Rescue Data
Comments

Expression of hepScer\UAS.cBa with Scer\GAL4ey-OK107 rescues the axonal phenotypes seen in hepr39 mushroom body clones.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
hepScer\UAS.cBa
hepUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Boutros
Secondary FlyBase IDs
    References (30)