Allele Dmel\gbb^Scer\UAS.cKa

General Information
Symbol	Dmel\gbb^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Khalsa	FlyBase ID	FBal0092971
Feature type	allele	Associated gene	Dmel\gbb

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Khalsa et al., 1998, Haerry et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A 1.6kb EcoRI fragment of a gbb cDNA (containing the entire open reading frame) is expressed under the control of Scer\UAS regulatory sequences. (Khalsa et al., 1998)
Carried in construct	P{UAS-gbb.K} (Baines, 2004, McCabe et al., 2003, Allan et al., 2003, Evans et al., 2009, Takaesu et al., 2005, Yu et al., 2004, Song et al., 2004, Kawase et al., 2004, Marques et al., 2003, Wharton, 2003.10.8, Yu et al., 2000, Khalsa et al., 1998, Haerry et al., 1998, Shivdasani and Ingham, 2003, Bangi and Wharton, 2006, Miller et al., 2011, Le and Wharton, 2012, Pentek et al., 2009, Ballard et al., 2010, Fradkin et al., 2008, Saja et al., 2010, James and Broihier, 2011, Ball et al., 2010, Fritsch et al., 2012, Serpe et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| third instar larval stage, with Scer\GAL4^D42 (James and Broihier, 2011) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (James and Broihier, 2011) neurophysiology defective, with Scer\GAL4^eve.RN2 (Baines, 2004, Fradkin et al., 2008) visible, with Scer\GAL4^71B (Khalsa et al., 1998) visible, with Scer\GAL4^A9 (Khalsa et al., 1998, Haerry et al., 1998, Yu et al., 2000, Bangi and Wharton, 2006, Le and Wharton, 2012) visible, with Scer\GAL4^en-e16E (Haerry et al., 1998) visible, with Scer\GAL4^ptc-559.1 (Khalsa et al., 1998, Evans et al., 2009, Kang and Bier, 2010) visible \| heat sensitive, with Scer\GAL4^en.PU, Scer\GAL80^ts.αTub84B (Fritsch et al., 2012)
Phenotype Manifest In
	1st posterior cell, with Scer\GAL4^ptc-559.1 (Khalsa et al., 1998) anterior crossvein, with Scer\GAL4^ptc-559.1 (Evans et al., 2009) bouton, with gbb²/gbb¹ (McCabe et al., 2003) synapse, with gbb²/gbb¹ (McCabe et al., 2003) synapse & neuromuscular junction, with gbb²/gbb¹ (McCabe et al., 2003) triple row, with Scer\GAL4^ap-md544 (Takaesu et al., 2005) triple row, with Scer\GAL4^C-765 (Takaesu et al., 2005) type I bouton \| supernumerary \| third instar larval stage, with Scer\GAL4^D42 (James and Broihier, 2011) type I bouton \| supernumerary \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (James and Broihier, 2011) wing, with Scer\GAL4^A9 (Vilmos et al., 2005, Khalsa et al., 1998, Haerry et al., 1998, Yu et al., 2000, James and Broihier, 2011, Le and Wharton, 2012) wing, with Scer\GAL4^ap-md544 (Takaesu et al., 2005) wing, with Scer\GAL4^C-765 (Takaesu et al., 2005) wing, with Scer\GAL4^ptc-559.1 (Khalsa et al., 1998) wing \| heat sensitive, with Scer\GAL4^en.PU, Scer\GAL80^ts.αTub84B (Fritsch et al., 2012) wing \| posterior compartment, with Scer\GAL4^en-e16E (Haerry et al., 1998) wing basal cell 1, with Scer\GAL4^ptc-559.1 (Khalsa et al., 1998) wing cell, with Scer\GAL4^A9 (Yu et al., 2000) wing cell, with Scer\GAL4^ptc-559.1 (Khalsa et al., 1998) wing disc, with Scer\GAL4^en-e16E (Haerry et al., 1998) wing vein, with Scer\GAL4^71B (Khalsa et al., 1998) wing vein, with Scer\GAL4^A9 (Khalsa et al., 1998, Bangi and Wharton, 2006) wing vein, with Scer\GAL4^ptc-559.1 (Kang and Bier, 2010) wing vein \| ectopic, with Scer\GAL4^71B (Khalsa et al., 1998) wing vein \| ectopic, with Scer\GAL4^A9 (Yu et al., 2000, Le and Wharton, 2012) wing vein \| ectopic, with Scer\GAL4^ptc-559.1 (Khalsa et al., 1998, Kang and Bier, 2010) wing vein L2, with Scer\GAL4^A9 (Haerry et al., 1998) wing vein L5, with Scer\GAL4^A9 (Haerry et al., 1998)
Detailed Description
	Statement Reference Expression of gbb[Scer\UAS.cKa] under the control of Scer\GAL4[en.PU] from the onset of pupal development (the temperature sensitive allele Scer\GAL80[ts.αTub84B] is used to restrict the stage during which expression occurs) results in wing defects which are more prominent in the posterior half. (Fritsch et al., 2012) Expression of gbb[Scer\UAS.cKa] under the control of Scer\GAL4[A9] results in a variable wing phenotype, marked by ectopic vein material. (Le and Wharton, 2012) Larvae expressing gbb[Scer\UAS.cKa] under the control of Scer\GAL4[D42] display a 40% increase in the number of boutons at neuromuscular junction (NMJ) 6/7 and a 62% increase in type I boutons at NMJ 4. Scer\GAL4[OK6]-mediated expression of gbb[Scer\UAS.cKa] has a comparable effect. Overexpression of gbb[Scer\UAS.cKa] using Scer\GAL4[A9] results in a severe wing blistering phenotype in 79% of females and a more severe phenotype, including unfurling and blistering of the wing in 93% of males. (James and Broihier, 2011) Expression of gbb[Scer\UAS.cKa] under the control of Scer\GAL4[ptc-559.1] results in an ectopic wing vein phenotype. (Kang and Bier, 2010) Expression of gbb[Scer\UAS.cKa] in the wing with Scer\GAL4[ptc-559.1] induces an expanded region of ectopic vein where the anterior crossvein would normally form. (Evans et al., 2009) Overexpression of gbb[Scer\UAS.cKa] post-synaptically under the control of Scer\GAL4[eve.RN2] increases synaptic current amplitude compared to controls. (Fradkin et al., 2008) Expression of gbb^Scer\UAS.cKa in the wing disc at 25^oC, under the control of Scer\GAL4^A9, produces wings divided into four classes of phenotypic severity according to the amount of extra wing vein material present in the wing. (Bangi and Wharton, 2006) The proximal 25% of the anterior margin of a gbb^Scer\UAS.cKa/Scer\GAL4^ap-md544 wing has no bristles. More distally, the triple row reappears but is quite irregular. When gbb^Scer\UAS.cKa is driven in all the cells of the wing blade with Scer\GAL4^C-765, the phenotype is similar but less severe. (Takaesu et al., 2005) Overexpression of gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9, can result in wing disruption. (Vilmos et al., 2005) Postsynaptic expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^eve.RN2, results in the averaged amplitude of synaptic currents recorded from whole-cell voltage clamp recordings of aCC/RP2 motoneurons being significantly increased compared with controls. In contrast, expression of gbb^Scer\UAS.cKa in presynaptic cholinergic interneurons, driven by Scer\GAL4^B19, does not affect the averaged synaptic current amplitude. (Baines, 2004) gbb^Scer\UAS.cKa; Scer\GAL4^C587 females do not have increased numbers of germ-line stem cells in their germaria. (Song et al., 2004) Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 produces slightly broadened wings consisting of solid veins. (Yu et al., 2000) Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^dpp.blk1 does not produce a mutant phenotype. Expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E results in overgrowth of the wing disc and defects restricted to the posterior compartment of the wing. (Haerry et al., 1998) Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^ptc-559.1 show ectopic vein material anterior to the anterior/posterior boundary in the wing, associated with a decrease in intervein tissue between veins L3 and L4. Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^71B have wing blisters and ectopic vein material. Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 show excessive vein formation in the wing. (Khalsa et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, enhanceable by Df(2R)H23/+ (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, enhanceable by sax⁴/sax[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, enhanceable by Scer\GAL4^A9/tkv^{Scer\UAS.T:Ivir\HA1} (Bangi and Wharton, 2006)
NOT Enhanced by
Statement Reference Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has visible phenotype, non-enhanceable by Scer\GAL4^ptc-559.1/kek4^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^71B, gbb^Scer\UAS.cKa has visible phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^71B (Khalsa et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^A9 (Khalsa et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible \| partially by Scer\GAL4^A9/sax^{Scer\UAS.T:Ivir\HA1} (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible \| partially by Scer\GAL4^A9/sax^{Scer\UAS.T:Zzzz\FLAG} (Le and Wharton, 2012) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible \| partially by Scer\GAL4^A9/tkv^{1ΔGSK.Scer\UAS} (Haerry et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible \| partially by tkv⁷/tkv[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Hsap\ACVR1^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^A9 (Le and Wharton, 2012) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Mad¹²/Mad[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Med[+]/Med¹ (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by put[+]/put¹³⁵ (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/cv-2^Scer\UAS.cSa (Serpe et al., 2008) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/sax^ΔI.Scer\UAS (Haerry et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/sog^Scer\UAS.cYa (Yu et al., 2000) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Scer\GAL4^A9/sog^{supersog1.Scer\UAS} (Yu et al., 2000) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by shn^3.3/shn[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by tkv⁵/tkv[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has visible phenotype, suppressible by tkv⁸/tkv[+] (Bangi and Wharton, 2006) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has visible phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^ptc-559.1 (Khalsa et al., 1998) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa (Kang and Bier, 2010, Kang and Bier, 2010) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/kek5^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/sog^Scer\UAS.cYa (Kang and Bier, 2010)
NOT suppressed by
Statement Reference Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has visible phenotype, non-suppressible by Scer\GAL4^ptc-559.1/kek4^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009)
Suppressor of
Statement Reference gbb^Scer\UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of neuroanatomy defective phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Saja et al., 2010) Scer\GAL4^how-24B, gbb^Scer\UAS.cKa, Scer\GAL4^how-24B is a suppressor of neuroanatomy defective \| embryonic stage phenotype of Scer\GAL4^how-24B/Scer\GAL4^how-24B, frac^Scer\UAS.cMa (Miller et al., 2011) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa (Kang and Bier, 2010, Kang and Bier, 2010)
NOT Suppressor of
Statement Reference gbb^Scer\UAS.cKa, Scer\GAL4^A9 is a non-suppressor of lethal phenotype of Fs^Scer\UAS.cPa, Scer\GAL4^A9 (Pentek et al., 2009) gbb^Scer\UAS.cKa/Scer\GAL4^eve.RN2 is a non-suppressor of neurophysiology defective \| heat sensitive phenotype of Cha^ts2 (Fradkin et al., 2008)
Other
Statement Reference gbb¹, gbb^Scer\UAS.cKa has neurophysiology defective \| heat sensitive phenotype (Baines, 2004)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, enhanceable by Df(2R)H23/+ (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, enhanceable by sax⁴/sax[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, enhanceable by Scer\GAL4^A9/tkv^{Scer\UAS.T:Ivir\HA1} (Bangi and Wharton, 2006)
NOT Enhanced by
Statement Reference Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has anterior crossvein phenotype, non-enhanceable by Scer\GAL4^ptc-559.1/kek4^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^71B, gbb^Scer\UAS.cKa has wing phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^71B (Khalsa et al., 1998) Scer\GAL4^71B, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^71B (Khalsa et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing cell phenotype, suppressible by Scer\GAL4^A9/sog^Scer\UAS.cYa (Yu et al., 2000) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible \| partially by cmpy^Scer\UAS.cJa/Scer\GAL4^A9 (James and Broihier, 2011) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^A9 (Khalsa et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible \| partially by Scer\GAL4^A9/sax^{Scer\UAS.T:Zzzz\FLAG} (Le and Wharton, 2012) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible \| partially by Scer\GAL4^A9/tkv^{1ΔGSK.Scer\UAS} (Haerry et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible by Hsap\ACVR1^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^A9 (Le and Wharton, 2012) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/cv-2^Scer\UAS.cSa (Serpe et al., 2008) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sax^ΔI.Scer\UAS (Haerry et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sog^Scer\UAS.cYa (Yu et al., 2000) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sog^Scer\UAS.cYa/tsg^Scer\UAS.cYa (Vilmos et al., 2005) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/sog^{supersog1.Scer\UAS} (Yu et al., 2000) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, suppressible by Scer\GAL4^A9/tsg^Scer\UAS.cYa (Vilmos et al., 2005) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein \| ectopic phenotype, suppressible by Scer\GAL4^A9/sog^Scer\UAS.cYa (Yu et al., 2000) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^A9 (Khalsa et al., 1998) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible \| partially by Scer\GAL4^A9/sax^{Scer\UAS.T:Ivir\HA1} (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible \| partially by tkv⁷/tkv[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible by Mad¹²/Mad[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible by Med[+]/Med¹ (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible by put[+]/put¹³⁵ (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible by shn^3.3/shn[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible by tkv⁵/tkv[+] (Bangi and Wharton, 2006) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible by tkv⁸/tkv[+] (Bangi and Wharton, 2006) Scer\GAL4^D42, gbb^Scer\UAS.cKa has type I bouton \| supernumerary \| third instar larval stage phenotype, suppressible by cmpy^Scer\UAS.cJa/Scer\GAL4^D42 (James and Broihier, 2011) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has anterior crossvein phenotype, suppressible by Scer\GAL4^ptc-559.1/kek5^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has wing phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^ptc-559.1 (Khalsa et al., 1998) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has wing vein \| ectopic phenotype, suppressible by Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa (Kang and Bier, 2010, Kang and Bier, 2010) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has wing vein \| ectopic phenotype, suppressible by Scer\GAL4^ptc-559.1/sog^Scer\UAS.cYa (Kang and Bier, 2010) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has wing vein phenotype, suppressible \| partially by dpp^Scer\UAS.cHa/Scer\GAL4^ptc-559.1 (Khalsa et al., 1998) Scer\GAL4^Rapgap1-OK6, gbb^Scer\UAS.cKa has type I bouton \| supernumerary \| third instar larval stage phenotype, suppressible by cmpy^Scer\UAS.cJa/Scer\GAL4^Rapgap1-OK6 (James and Broihier, 2011)
NOT suppressed by
Statement Reference Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing phenotype, non-suppressible by Scer\GAL4^A9/cv^Scer\UAS.cVa (Vilmos et al., 2005) Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype, non-suppressible by Xlae\noggin^Scer\UAS.cYa/Scer\GAL4^A9 (Yu et al., 2000) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa has anterior crossvein phenotype, non-suppressible by Scer\GAL4^ptc-559.1/kek4^{Scer\UAS.T:Avic\GFP} (Evans et al., 2009)
Suppressor of
Statement Reference gbb^Scer\UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Saja et al., 2010) gbb^Scer\UAS.cKa/Scer\GAL4^ap-md544 is a suppressor of embryonic/larval neuron phenotype of Scer\GAL4^ap-md544, robo^Scer\UAS.cKa (Allan et al., 2003) gbb^Scer\UAS.cKa/Scer\GAL4^ap-md544 is a suppressor of ventral nerve cord phenotype of Scer\GAL4^ap-md544, robo^Scer\UAS.cKa (Allan et al., 2003) Scer\GAL4^how-24B, gbb^Scer\UAS.cKa, Scer\GAL4^how-24B is a suppressor of intersegmental nerve branch ISNb of A1-7 \| embryonic stage phenotype of Scer\GAL4^how-24B/Scer\GAL4^how-24B, frac^Scer\UAS.cMa (Miller et al., 2011) Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa is a suppressor of wing vein \| ectopic phenotype of Scer\GAL4^ptc-559.1, gbb^Scer\UAS.cKa (Kang and Bier, 2010, Kang and Bier, 2010)
Other
Statement Reference Hsap\ACVR1^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^A9, gbb^Scer\UAS.cKa has wing vein phenotype (Le and Wharton, 2012) Scer\GAL4^A9, cv^Scer\UAS.cVa, gbb^Scer\UAS.cKa, sog^Scer\UAS.cYa has crossvein phenotype (Vilmos et al., 2005, Vilmos et al., 2005, Vilmos et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference The severity of the wing phenotype caused by expression of gbb[Scer\UAS.cKa] under the control of Scer\GAL4[A9] is reduced by co-expression of sax[Scer\UAS.T:Zzzz\FLAG]. (Le and Wharton, 2012) Co-expression of cmpy[Scer\UAS.cJa] suppresses the neuromuscular overgrowth phenotype caused by expression of gbb[Scer\UAS.cKa] using either Scer\GAL4[D42] or Scer\GAL4[OK6]. Co-expression of cmpy[Scer\UAS.cJa] suppresses the wing blistering phenotype caused by Scer\GAL4[A9]-mediated expression of gbb[Scer\UAS.cKa] in males and females. (James and Broihier, 2011) Mesodermal expression of gbb[Scer\UAS.cKa] (under the control of Scer\GAL4[how-24B]) suppresses the phenotype observed with frac[Scer\UAS.cMa] over-expression (48% to 28%). (Miller et al., 2011) Co-expression of either sog[Scer\UAS.cYa] or Hip14[Scer\UAS.cKa] suppresses the ectopic wing vein phenotype caused by expression of gbb[Scer\UAS.cKa] under the control of Scer\GAL4[ptc-559.1]. (Kang and Bier, 2010) Overexression of gbb[Scer\UAS.cKa] with Ppt1[Scer\UAS.cKa], both under the control of Scer\GAL4[GMR.PF] suppresses the visual system degeneration seen upon Ppt1[Scer\UAS.cKa] expression alone. (Saja et al., 2010) The ectopic vein phenotype in the area where the anterior crossvein normally forms resulting from the Scer\GAL4[ptc-559.1] driven expression of gbb[Scer\UAS.cKa] can be suppressed by co-expressing kek5[Scer\UAS.T:Avic\GFP] in the wing. The ectopic vein phenotype in the area where the anterior crossvein normally forms resulting from the Scer\GAL4[ptc-559.1] driven expression of gbb[Scer\UAS.cKa] is not influenced by co-expressing kek4[Scer\UAS.T:Avic\GFP] in the wing. (Evans et al., 2009) Overexpression of gbb[Scer\UAS.cKa] post-synaptically under the control of Scer\GAL4[eve.RN2] fails to suppress the reduced synaptic currents found in Cha[ts2] mutant second instar larvae raised at 18[o]C. (Fradkin et al., 2008) Expression of cv-2[Scer\UAS.cSa] suppresses the wing phenotypes seen when gbb[Scer\UAS.cKa] is expressed under the control of Scer\GAL4[A9]. (Serpe et al., 2008) Coexpression of gbb^Scer\UAS.cKa in the wing disc with sax^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^A9, results in an enhancement of the Scer\GAL4^A9>gbb^Scer\UAS.cKa wing vein phenotype; all wings show the mildest form of vein phenotype in the gbb^Scer\UAS.cKa-sax^{Scer\UAS.T:Ivir\HA1} coexpressing wings, while less than 10% of wings show this mild phenotype in gbb^Scer\UAS.cKa wings. Expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^A9, in a sax⁴/+ background partially suppresses the extra wing vein phenotype; over 90% of flies expressing both UAS transgenes show the most severe form of the Scer\GAL4^A9>gbb^Scer\UAS.cKa phenotype, compared to about 20% of Scer\GAL4^A9>gbb^Scer\UAS.cKa flies. Coexpression of tkv^{Scer\UAS.T:Ivir\HA1} and gbb^Scer\UAS.cKa enhances the Scer\GAL4^A9>gbb^Scer\UAS.cKa wing vein phenotype so that a higher percentage of wings have extensive amounts of wing vein tissue. Expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^A9, in a tkv⁷/+ background suppresses the extra wing vein phenotype of Scer\GAL4^A9>gbb^Scer\UAS.cKa flies. Around 80% of Scer\GAL4^A9>gbb^Scer\UAS.cKa; tkv⁷/+ wings show a very mild vein phenotype, while around 20% appear wild type. (Bangi and Wharton, 2006) Co-expression of cv^Scer\UAS.cVa with gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 has no detectable effect on the overexpression phenotype of gbb^Scer\UAS.cKa (wing disruption). Co-expression of cv^Scer\UAS.cVa and sog^Scer\UAS.cYa, with gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 re-establishes the intervein tissue and the longitudinal veins with a series of expanded crossveins between L2 and L3 on the wing. Therefore, cv and sog interact together to suppress the wing phenotype of gbb. Co-expression of tsg^Scer\UAS.cYa and sog^Scer\UAS.cYa, along with gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 partially suppresses the excess gbb^Scer\UAS.cKa wing disruption phenotype. Co-expression of tsg^Scer\UAS.cYa and gbb^Scer\UAS.cKa, under the regulation of Scer\GAL4^A9 suppresses the excess gbb phenotype. (Vilmos et al., 2005) Postsynaptic expression of gbb^Scer\UAS.cKa, under the control of Scer\GAL4^eve.RN2, fails to potentiate synaptic current amplitude in aCC/RP2 neurons when expressed in a Cha^ts2 homozygous background at 29^oC, compared to in a Cha^ts2/+ control background. If larvae are allowed to recover at 20^oC for another 22 hours, the ability of gbb^Scer\UAS.cKa to potentiate synaptic current amplitude in a Cha^ts2 background is restored. (Baines, 2004) The solid vein phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is strongly suppressed by co-expression of sog^Scer\UAS.cYa or of sog^{supersog1.Scer\UAS}. (Yu et al., 2000) Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 at 25^oC have slightly larger wings than normal with minor venation defects along veins L2 and L5. Flies expressing gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 at 30^oC have blistered and highly pigmented wings. The wing phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is partially suppressed if the flies are also coexpressing tkv^{1ΔGSK.Scer\UAS}, and is almost completely suppressed if the flies are also coexpressing sax^ΔI.Scer\UAS. (Haerry et al., 1998)
Xenogenetic Interactions
Statement Reference The wing phenotype caused by expression of gbb[Scer\UAS.cKa] under the control of Scer\GAL4[A9] is suppressed by co-expression of Hsap\ACVR1[Scer\UAS.T:Zzzz\FLAG] and the resulting flies have a reduction in wing size and a loss of wing vein material. (Le and Wharton, 2012) The solid vein phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is not suppressed by co-expression of Xlae\noggin^Scer\UAS.cYa. (Yu et al., 2000)
Complementation & Rescue Data
Rescues	Scer\GAL4^fat/gbb^Scer\UAS.cKa rescues gbb¹ (Ballard et al., 2010) Scer\GAL4^{nos.UTR.T:Hsim\VP16}/gbb^Scer\UAS.cKa rescues gbb⁴ (Shivdasani and Ingham, 2003)
Partially rescues	gbb^Scer\UAS.cKa/Scer\GAL4[-] partially rescues gbb²/gbb¹ (McCabe et al., 2003) gbb^Scer\UAS.cKa/Scer\GAL4^hs.PB partially rescues gbb⁴/gbb¹ (Khalsa et al., 1998) Scer\GAL4^BG380/gbb^Scer\UAS.cKa partially rescues gbb²/gbb¹ (McCabe et al., 2003) Scer\GAL4^elav-C155/gbb^Scer\UAS.cKa partially rescues gbb²/gbb¹ (McCabe et al., 2003) Scer\GAL4^G14/gbb^Scer\UAS.cKa partially rescues gbb²/gbb¹ (McCabe et al., 2003) Scer\GAL4^how-24B, gbb^Scer\UAS.cKa, Scer\GAL4^how-24B partially rescues gbb⁴/gbb¹ (Khalsa et al., 1998)
Comments	The phenotypes associated with homozygous gbb[1] mutants (including lethality, larval transparency and abnormal fatty acid uptake and transport to the fat body) are rescued by expression of gbb[Scer\UAS.cKa] driven by Scer\GAL4[fat]. (Ballard et al., 2010) The presence of P{UAS-gbb.K}9.9 alone (in the absence of any driver) can rescue survival of gbb¹/gbb² animals to 3rd instar or pupal stage. The additional presence of Scer\GAL4^G14 rescues bouton density and spontaneous mini-EJP (excitatory junctional potential) frequency to wild-type levels, and partially rescues spontaneous mini-EJP amplitude, evoked EJP frequency and levels of neurotransmitter release at synapses. Essentially identical results are seen when Scer\GAL4^BG380 is used instead (although bouton density not measured). However, when Scer\GAL4^elav-C155 is used there is only a slight rescue of bouton density, but the frequency and amplitude of spontaneous mini-EJPs, the amplitude of evoked EJPs and the levels of neurotransmitter release at synapses are all rescued to wild-type levels. (McCabe et al., 2003) The testis defects seen in gbb⁴ homozygous males are rescued by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. (Shivdasani and Ingham, 2003)
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Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	gbb^Scer\UAS.cKa gbb^UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Khalsa (Khalsa et al., 1998)
Secondary FlyBase IDs

References ( 28 )

Generate a list of	All references relating to this allele ( 28 )

List References by type	Research paper ( 25 ) Supplementary material ( 2 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 4 )
	Fritsch et al., 2012, J. Biol. Chem. 287(8): 5942--5953 Different Requirements for Proteolytic Processing of Bone Morphogenetic Protein 5/6/7/8 Ligands in Drosophila melanogaster. [FBrf0217533] Le and Wharton, 2012, Dev. Dyn. 241(1): 200--214 Hyperactive BMP signaling induced by ALK2(R206H) requires type II receptor function in a Drosophila model for classic fibrodysplasia ossificans progressiva. [FBrf0217004] James and Broihier, 2011, Development 138(15): 3273--3286 Crimpy inhibits the BMP homolog Gbb in motoneurons to enable proper growth control at the Drosophila neuromuscular junction. [FBrf0214390] Miller et al., 2011, J. Neurosci. 31(14): 5335--5347 Drosophila mmp2 regulates the matrix molecule faulty attraction (frac) to promote motor axon targeting in Drosophila. [FBrf0213409] Show all research papers ...

Allele Dmel\gbbScer\UAS.cKa

Allele Dmel\gbb^Scer\UAS.cKa