cDNA sequence nucleotides 589-1413 (FBrf0050626) is expressed from UASt regulatory sequences. This includes coding sequence for the N-terminal 272 amino acids which encompasses the first transmembrane domain and the first two residues of the first intracellular loop, and was put in frame with a heterologous RWL peptide followed by a stop codon. Contains 9 nucleotides of 5' UTR.
Expression of fzScer\UAS.N and fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4dome-PG14 results in mislocalisation of hemocyte precursors from the medullary zone into the cortical zone of the lymph gland, and differentiation of hemocyte precursors into plasmatocytes is observed.
The phenotype caused by expression of fzScer\UAS.N using Scer\GAL469B can be rescued by co-expression with fz2Scer\UAS.cZa. This phenotype can be dominantly suppressed by wgl-17. When expression is driven by Scer\GAL469B, ectopic wing bristle phenotype of fz2Scer\UAS.cZa is suppressed.