Allele Dmel\dsh^hs.sev.B

General Information
Symbol	Dmel\dsh^hs.sev.B	Species	D. melanogaster
Name		FlyBase ID	FBal0093042
Feature type	allele	Associated gene	Dmel\dsh

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Boutros et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of dsh cDNA is driven by an Hsp70 promoter and sev enhancer. (Boutros et al., 1998)
Carried in construct	P{sE-Dsh.B} (Paricio et al., 1999, Weber et al., 2008, Pazman et al., 2000, Weber et al., 2000, Blaumueller and Mlodzik, 2000, Fanto and Mlodzik, 1999, Boutros et al., 1998, Jenny et al., 2005, Weber et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	planar polarity defective (Paricio et al., 1999, Fanto and Mlodzik, 1999, Pazman et al., 2000, Weber et al., 2008, Weber et al., 2012) planar polarity defective \| dominant \| heat sensitive (Boutros et al., 1998) wild-type (Boutros et al., 1998)
Phenotype Manifest In
	ommatidium (Paricio et al., 1999, Fanto and Mlodzik, 1999, Weber et al., 2008, Weber et al., 2012) ommatidium \| heat sensitive (Boutros et al., 1998) photoreceptor cell R4 (Fanto and Mlodzik, 1999)
Detailed Description
	Statement Reference Expression of dsh[hs.sev.B] results in ommatidial orientation defects in the eye. (Weber et al., 2012) Expression of dsh[hs.sev.B] results in 42% of adult ommatidia having a symmetrical arrangement. (Weber et al., 2008) Ommatidial arrangement is disrupted due to overactivation of planar polarity signalling. (Paricio et al., 1999) Eyes of flies raised at 29^oC show disruption of ommatidial arrangement with random or no chirality and misrotations of single ommatidia. (Boutros et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference dsh^hs.sev.B has planar polarity defective phenotype, enhanceable by Dl^RevF10/Dl[+] (Fanto and Mlodzik, 1999) dsh^hs.sev.B has planar polarity defective phenotype, enhanceable by N[+]/N^264-39 (Fanto and Mlodzik, 1999) dsh^hs.sev.B has visible phenotype, enhanceable by ex[+]/ex^e1 (Blaumueller and Mlodzik, 2000)
NOT Enhanced by
Statement Reference dsh^hs.sev.B has planar polarity defective phenotype, non-enhanceable by dock³ (Paricio et al., 1999)
Suppressed by
Statement Reference dsh^hs.sev.B has planar polarity defective phenotype, suppressible \| partially by aop[+]/aop^e2d (Weber et al., 2008) dsh^hs.sev.B has planar polarity defective phenotype, suppressible \| partially by aop^yan-XE18/aop[+] (Weber et al., 2008) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by +/Df(3R)ED5644 (Weber et al., 2012) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by bsk²/bsk[+] (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by CG15283^GD13108/Scer\GAL4^hs.2sev (Weber et al., 2012) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(1)G24/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(1)H6/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(2L)b80e3/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(2L)b87e25/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(2L)ED793/+ (Weber et al., 2012) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(2L)flp170B/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(3R)crb87-4/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(3R)crb-F89-4/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Df(3R)p13/+ (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by hep[+]/hep^r75 (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Jra¹/Jra[+] (Weber et al., 2008) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by Jra^IA109/Jra[+] (Weber et al., 2008) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by kay¹/kay[+] (Weber et al., 2008) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by kay^ED6315/kay[+] (Weber et al., 2008) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by msn[+]/msn¹⁰² (Paricio et al., 1999) dsh^hs.sev.B has planar polarity defective phenotype, suppressible by msn¹⁷²/msn[+] (Paricio et al., 1999)
NOT suppressed by
Statement Reference dsh^hs.sev.B has planar polarity defective phenotype, non-suppressible by dock³ (Paricio et al., 1999)
Phenotype Manifest In
Enhanced by
Statement Reference dsh^hs.sev.B has eye phenotype, enhanceable by ex[+]/ex^e1 (Blaumueller and Mlodzik, 2000) dsh^hs.sev.B has eye photoreceptor cell phenotype, enhanceable by ex[+]/ex^e1 (Blaumueller and Mlodzik, 2000) dsh^hs.sev.B has ommatidium phenotype, enhanceable by Dl^RevF10/Dl[+] (Fanto and Mlodzik, 1999) dsh^hs.sev.B has ommatidium phenotype, enhanceable by ex[+]/ex^e1 (Blaumueller and Mlodzik, 2000) dsh^hs.sev.B has ommatidium phenotype, enhanceable by N[+]/N^264-39 (Fanto and Mlodzik, 1999) dsh^hs.sev.B has photoreceptor cell R4 phenotype, enhanceable by Dl^RevF10/Dl[+] (Fanto and Mlodzik, 1999) dsh^hs.sev.B has photoreceptor cell R4 phenotype, enhanceable by N[+]/N^264-39 (Fanto and Mlodzik, 1999)
NOT Enhanced by
Statement Reference dsh^hs.sev.B has ommatidium phenotype, non-enhanceable by dock³ (Paricio et al., 1999) dsh^hs.sev.B has phenotype, non-enhanceable by rin^unspecified (Pazman et al., 2000)
Suppressed by
Statement Reference dsh^hs.sev.B has ommatidium phenotype, suppressible \| partially by aop[+]/aop^e2d (Weber et al., 2008) dsh^hs.sev.B has ommatidium phenotype, suppressible \| partially by aop^yan-XE18/aop[+] (Weber et al., 2008) dsh^hs.sev.B has ommatidium phenotype, suppressible by +/Df(3R)ED5644 (Weber et al., 2012) dsh^hs.sev.B has ommatidium phenotype, suppressible by bsk²/bsk[+] (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by CG15283^GD13108/Scer\GAL4^hs.2sev (Weber et al., 2012) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(1)G24/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(1)H6/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(2L)b80e3/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(2L)b87e25/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(2L)ED793/+ (Weber et al., 2012) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(2L)flp170B/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(3R)crb87-4/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(3R)crb-F89-4/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Df(3R)p13/+ (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by hep[+]/hep^r75 (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by Jra¹/Jra[+] (Weber et al., 2008) dsh^hs.sev.B has ommatidium phenotype, suppressible by Jra^IA109/Jra[+] (Weber et al., 2008) dsh^hs.sev.B has ommatidium phenotype, suppressible by kay¹/kay[+] (Weber et al., 2008) dsh^hs.sev.B has ommatidium phenotype, suppressible by kay^ED6315/kay[+] (Weber et al., 2008) dsh^hs.sev.B has ommatidium phenotype, suppressible by msn[+]/msn¹⁰² (Paricio et al., 1999) dsh^hs.sev.B has ommatidium phenotype, suppressible by msn¹⁷²/msn[+] (Paricio et al., 1999) dsh^hs.sev.B has phenotype, suppressible by bsk² (Boutros et al., 1998) dsh^hs.sev.B has phenotype, suppressible by hep^r75 (Boutros et al., 1998) dsh^hs.sev.B has phenotype, suppressible by Jra¹ (Boutros et al., 1998) dsh^hs.sev.B has phenotype, suppressible by Jra^IA109 (Boutros et al., 1998) dsh^hs.sev.B has phenotype, suppressible by Rho1^k07903 (Boutros et al., 1998)
NOT suppressed by
Statement Reference dsh^hs.sev.B has ommatidium phenotype, non-suppressible by dock³ (Paricio et al., 1999) dsh^hs.sev.B has phenotype, non-suppressible by arm² (Boutros et al., 1998) dsh^hs.sev.B has phenotype, non-suppressible by arm⁸ (Boutros et al., 1998) dsh^hs.sev.B has phenotype, non-suppressible by Cdc42³ (Boutros et al., 1998) dsh^hs.sev.B has phenotype, non-suppressible by pan^ER1 (Boutros et al., 1998) dsh^hs.sev.B has phenotype, non-suppressible by Ras85D^e2F (Boutros et al., 1998) dsh^hs.sev.B has phenotype, non-suppressible by rin^unspecified (Pazman et al., 2000) dsh^hs.sev.B has phenotype, non-suppressible by rl¹⁰ (Boutros et al., 1998) dsh^hs.sev.B has phenotype, non-suppressible by wg^l-17 (Boutros et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference The dsh[hs.sev.B] gain-of-function planar cell polarity (PCP)-phenotype is markedly suppressed by the expression of CG15283[GD13108] via Scer\GAL4[hs.2sev]. (Weber et al., 2012) One copy of kay[1], kay[ED6315], Jra[1] or Jra[IA109] suppresses the formation of symmetrical ommatidia that is seen in animals expressing dsh[hs.sev.B]. One copy of aop[yan-XE18] or aop[e2d] partially suppresses the formation of symmetrical ommatidia that is seen in animals expressing dsh[hs.sev.B]. (Weber et al., 2008) The eye phenotype seen in dsh^hs.sev.B flies is dominantly enhanced by the addition of ex^e1 due to an increase in unscorable ommatidia (missing one or more photoreceptors. (Blaumueller and Mlodzik, 2000) The tissue polarity phenotype of flies carrying dsh^hs.sev.B is dominantly enhanced by N^264-39 or Dl^RevF10; the percentage of symmetrical ommatidia containing an R3/R3 pair in the normal R3/R4 position is increased. (Fanto and Mlodzik, 1999) The ommatidial polarity defects seen in dsh^hs.sev.B flies grown at 29^oC can be dominantly suppressed by the addition of hep^r75, msn¹⁰², msn¹⁷², bsk², Df(1)H6, Df(1)G24, Df(2L)flp170B, Df(3R)crb87-4, Df(2L)b80e3, Df(3R)p13, Df(3R)crb-F89-4 and Df(2L)b87e25. Between 70-90% of ommatidia are in their correct polarity, compared with 55% in dsh^hs.sev.B alone. The ommatidial polarity defects seen in dsh^hs.sev.B flies grown at 29^oC are not suppressed by the addition of dock³. (Paricio et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	dsh^hs.sev.B rescues dsh¹ (Boutros et al., 1998) dsh^hs.sev.B rescues dsh³ (Boutros et al., 1998)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	dsh^hs.sev.B
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Weber et al., 2012, Genetics 191(1): 145--162 Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] Weber et al., 2008, Dev. Biol. 316(1): 110--123 Combinatorial signaling by the Frizzled/PCP and Egfr pathways during planar cell polarity establishment in the Drosophila eye. [FBrf0204109] Jenny et al., 2005, Nat. Cell Biol. 7(7): 691--697 Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. [FBrf0188147] Blaumueller and Mlodzik, 2000, Mech. Dev. 92(2): 251--262 The Drosophila tumor suppressor expanded regulates growth, apoptosis, and patterning during development. [FBrf0127012] Pazman et al., 2000, Development 127(8): 1715--1725 Rasputin, the Drosophila homologue of the RasGAP SH3 binding protein, functions in ras- and -mediated signaling. [FBrf0127269] Weber et al., 2000, Development 127(16): 3619--3629 Jun mediates Frizzled-induced R3/R4 cell fate distinction and planar polarity determination in the Drosophila eye. [FBrf0128678] Fanto and Mlodzik, 1999, Nature 397(6719): 523--526 Asymmetric Notch activation specifies photoreceptors R3 and R4 and planar polarity in the Drosophila eye. [FBrf0106469] Paricio et al., 1999, EMBO J. 18(17): 4669--4678 The Drosophila STE20-like kinase misshapen is required downstream of the frizzled receptor in planar polarity signaling. [FBrf0111453] Boutros et al., 1998, Cell 94(1): 109--118 Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. [FBrf0103240]

Allele Dmel\dshhs.sev.B

Allele Dmel\dsh^hs.sev.B