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General Information
Symbol
Dmel\MycUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zaffran
FlyBase ID
FBal0093088
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dmyc, UAS-Myc, UAS-dm, UASdmyc, UAS-dmyc42
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a Myc cDNA.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
follicle cell & nucleus | somatic clone, with Scer\GAL4Act5C.PP
larval salivary gland & nucleolus, with Scer\GAL4en-e16E
Detailed Description
Statement
Reference
Expressing MycUAS.cZa under the control of Scer\GAL4ptc-559.1 leads to increased ploidy and endoreplication (assessed by EdU labelling) in the larval salivary glands, as compared to controls.
Very few animals expressing MycScer\UAS.cZa under the control of either Scer\GAL4c42 or Scer\GAL4tsh-c724 survive to adulthood, the rare escapers display severely deformed Malpighian tubules - swollen and disorganized but no extra branching.
The expression of MycScer\UAS.cZa under the control of Scer\GAL4en-e16E leads to a significant increase in the wing posterior compartment area, as compared to controls.
Secondary neuroblasts in larval brains expressing MycScer\UAS.cZa under the control of Scer\GAL4insc-Mz1407 exhibit increased nucleolar size, as compared to wild type.
The expression of MycScer\UAS.cZa under the control of Scer\GAL4E(spl)m6-BFM.PF leads to significantly increased numbers of abdominal lateral and dorsal adult, but not ventral, muscle precursor cells in third instar larvae, as compared to controls.
Clones expressing dmScer\UAS.cZa (using P{CoinFLP-GAL4} and Scer\FLP1ey.PN to stochastically generate patches of cells expressing Scer\GAL4Act5C.CoinFLP-FRT) are over-represented in the adult eye compared to control cells. Expression of dmScer\UAS.cZa using P{AyGAL4} and Scer\FLP1ey.PN to generate clones in the eye expressing Scer\GAL4Act5C.PI results in a slight overgrowth of the eye.
Scer\GAL4ptc-559.1-mediated expression of MycScer\UAS.cZa dramatically increases the size of the nucleolus and rough endoplasmic reticulum in salivary gland cells. Clonal expression of MycScer\UAS.cZa in the fat body via Scer\GAL4Scer\FRT.Rnor\CD2.Act5C flip-out technique results in increased cell size (up to 50%) and an increased frequency of endoreplicating cells. Scer\GAL4ptc-559.1-mediated expression of MycScer\UAS.cZa extends the endoreplicating period of salivary gland cells.
There is an increase in the frequency of 'winner' clone splitting (used as a readout for winner-loser cell mixing) when cell competition is induced through generation of super-competitive clones (expressing MycScer\UAS.cZa under the control of Scer\GAL4Scer\FRT.Act5C, and in which apoptosis has been inhibited) in wild type tissues compared with wild type clones in wild type tissue.
Expression of MycScer\UAS.cZa under the control of Scer\GAL4ptc.PU in cells of the wing disc proper has no significant effect on the morphology or proliferation of the overlying peripodial membrane cells in third instar larvae.
Expression of MycScer\UAS.cZa in terminal cells under the control of Scer\GAL4bs-1348 has no effect on the number of thicker terminal branches in the dorsal and lateral G branches of the larval trachea, but increases the formation of fine terminal branches near the terminal cell body compared with controls. Some of the thick terminal branches appear more torturous and the air-filled lumens are uneven in size.
The expression of MycScer\UAS.cZa under the control of Scer\GAL4nub.PU has no obvious effect on the wing size of individuals reared at 25[o]C temperature, as compared to controls.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 leads to overproliferation of the wing disc epithelium and some invasion of cells.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4nub-AC-62 has no effect on wing size.
Five days after heat-shock induction of dm (through expression of dmScer\UAS.cZa at 37[o]c with Scer\GAL4hs.PU), the number of dpn-positive cells is significantly increased compared to control flies, indicating that there is an increase in neurogenesis.
Overexpression of Scer\GAL4Act5C.PP>dmScer\UAS.cZa during larval development increases the frequency of somatic mutations. Overexpression of dmScer\UAS.cZa at 29[o]C under the control of Scer\GAL4Act.PU and Scer\GAL80ts.αTub84B shortens median lifespan from 38 days in controls to 22 days. dmScer\UAS.cZa overexpression shortens lifespan at 25[o]C from a median of 58 days to 31 days.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4insc-Mz1407 does not result in a significant change in the number of type II central brain neuroblasts compared to wild type.
Expression of three copies of P{UAS-dm.Z} via Scer\GAL4GMR.PU results in a rough eye: eyes display disorganized ommatidia and are larger than wild type. Scer\GAL4Bx-MS1096-KE-mediated expression of dmScer\UAS.cZa causes the adult wing to curve downwards (indicative of increased cell size in the dorsal layer). Apoptosis is not observed when dmScer\UAS.cZa is expressed from Scer\GAL4Bx-MS1096-KE or Scer\GAL4en.PU. Scer\GAL4en.PU-mediated expression of dmScer\UAS.cZa significantly increases the area of the posterior compartment of the wing, as well as the ratio between the posterior and anterior areas, owing to enlarged cell size. Scer\GAL4en.PU-mediated expression of dmScer\UAS.cZa does not generate a wing vein phenotype.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4tey-5053A has no effect on the number of tracheal branches that terminate on ventrolateral body wall muscle (VLM) 12, despite a 28.5% increase in the 2-dimensional size of the VLM12 muscle.
Overexpression of dmScer\UAS.cZa in larval fat body clones results in increased cell growth compared with neighboring control cells. Overexpression of dmScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 induces cell autonomous overgrowth in the developing wing disc resulting in increased L3-L4 wing vein distance in the adult.
Overexpression of dmScer\UAS.cZa in subperineurial glia, under the control of Scer\GAL4moody.PS results in an increase in polyploidy in these cells.
Late third instar wing discs expressing dmScer\UAS.cZa under the control of Scer\GAL4sd-SG29.1 have disturbed dorsal-ventral (DV) boundaries compared to controls.
Over-expression of dmScer\UAS.cZa using Scer\GAL4sca-109-68 increases the nuclear and nucleolar size of the posterior scutellar shaft cells.
Eye disc clones expressing dmScer\UAS.cZa are significantly larger than control clones as a result of increased cell size. Salivary glands expressing dmScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 have dramatically increased nuclear, nucleolar and overall cell size. Nucleoli display slightly less compact packaging with closely intermingled fibrillar and granular components.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4repo.PU slightly increases glial cell numbers in the third instar larval eye disc and brain.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in flies with larger ommatidia than normal. Clones in the wing disc expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP are increased in size compared to control clones. Clones in the larval salivary glands expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP result in excessive endoreplication.
Mosaic dmScer\UAS.cZa-Scer\GAL4hh.PU clones tend to die and do not overgrow compared to wild-type twin clones.
Many animals expressing dmScer\UAS.cZa under the control of Scer\GAL4da.G32 hatch into viable larvae, however these larvae fail to grow and after approximately 3 days, they die as small larvae. These larvae behave as wandering third instars, and often die attached to the side of the vial.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4repo.PU results in larval brains that contain many glia with enlarged nuclei.
Flies expressing three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF have larger eyes than normal which contain larger than normal ommatidia. The eyes have a rough exterior. Third larval instar eye discs expressing three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF show a small, but statistically significant, increase in apoptotic cells posterior to the morphogenetic furrow compared to controls. Expression of dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 results in increased apoptosis in the domain of Scer\GAL4ap-md544 expression.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP in primary cell cultures of cells derived from embryos does not produce very large clones of cells.
Expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in apoptosis, resulting in a disturbance of the internal architecture of the adult eye and reducing the number of pigment cells it contains. The average ommatidial size is increased in these animals compared to controls.
Expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in apoptosis in the eye and an increase in average ommatidial size compared to controls.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4en-e16E has no effect on the overall size of the posterior wing compartment, but cell number is reduced and the size of the cells is increased.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in an increase in eye size.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4fkh.PH does not prevent the salivary gland degradation that is seen during the pupal stage in normal development.
Cells that are expressing dmScer\UAS.cZa under the control of Scer\GAL4en-e16E engulf adjacent apoptotic wild-type cells.
Expression of dmScer\UAS.cZa in the developing tracheal system under the control of Scer\GAL4btl.PS does not affect tracheal development.
Stage 9 embryos expressing dmScer\UAS.cZa under the control of Scer\GAL4rho.PU show neuroblast hyperplasia and elevated mitotic activity in the developing neuroectoderm.
Flies expressing dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF have eyes that are larger than normal and which have a rough appearance due to disorganised ommatidia. Each ommatidium is enlarged 33% compared to wild type, and this results in a disruption of the ommatidial array. Eye discs expressing dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF do not show defects in neuronal differentiation, and the pattern of cells in S phase and apoptosis is wild type. The photoreceptor cells are dramatically enlarged compared to wild type.
Clones of cells in the third larval instar fat body expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP contain more DNA than wild-type controls.
Females expressing dmScer\UAS.cZa under the control of Scer\GAL4dm-PG45 show reduced viability at 18 and 22[o]C (with greater survival at 18[o]C) and do not survive to adulthood at 26[o]C. Clones in the wing disc expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP show an increase in cell size and a strong reduction in the fraction of cells in G[[1]] with a concomitant increase in cells in S or G[[2]] phase compared to controls. The clones show high levels of apoptosis. Expression of dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 induces apoptosis in the wing disc.
Expression of dmScer\UAS.cZa in the ring gland using Scer\GAL4P0206 induces autonomous growth.
When dmScer\UAS.cZa is driven by Scer\GAL4Act5C.PP in clones, growth stimulation is seen. Mitochondrial activity is not stimulated. When mitochondrial inhibitors are used a slight increase in growth stimulation is seen in fat cells. Hypoxia treatment has no effect.
dmScer\UAS.cZa overexpressing cells, under the regulation of Scer\GAL4en-e16E have enlarged nucleoli compared with surrounding wild-type cells. Overexpression of dmScer\UAS.cZa in salivary gland cells under the regulation of Scer\GAL4ptc-559.1 leads to a loss of secretory vesicles, and an enlarged nucleus and nucleolus. Although dmScer\UAS.cZa expression increases ploidy in these cells, the ratio of nucleolar volume to DNA content is increased approximately sixfold by dm. dmScer\UAS.cZa-expressing cells have a dense cytoplasm, characterised by an increase in the number and density of ribosomes and polysomes. In addition, an extensive network of rough endoplasmic reticulum is observed specifically in these cells, compared to wild-type. dmScer\UAS.cZa clones generated in the wing disc using the MARCM GAL repressor/activator system, undergo an increase in cell size compared to wild-type. Overexpression of dmScer\UAS.cZa also promotes G1/S phase transitions in wing disc cells, leading to a decreased population of G1 cells and an increase in the proportion of S/G2 phase cells.
Clones in the eye disc expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP result in larger cells than normal.
Expression of dmScer\UAS.cZa when driven by Scer\GAL4C96 prevents the block to G1-S phase transition in the nonproliferating cells (ZNC) of third instar larvae.
Nuclei in dmScer\UAS.cZa; Scer\GAL4Act5C.PP somatic clones in the 2nd or 3rd instar larval fat body have significantly higher DNA content (assayed by DAPI fluorescence) than neighbouring cells. The magnitude of this difference increases with age. Unlike wild-type, significant levels of endoreplication occur at the wandering third instar stage in dmScer\UAS.cZa; Scer\GAL4ptc-559.1 salivary glands & dmScer\UAS.cZa; Scer\GAL4Adh.PF fat bodies. (Assayed by BrdU incorporation).
Cells are larger than wild-type posterior to the morphogenetic furrow in dmScer\UAS.cZa; Scer\GAL4GMR.PF third instar larval eye discs, and the bands of cells in S phase band and in mitosis posterior to the furrow are thinner than in wild type. The resulting adults have larger adult ommatidia and oversized eyes.
In somatic clones of dmScer\UAS.cZa; Scer\GAL4Act5C.PP cells in the ovarian follicle cells, nuclei become enlarged. FACS profiles of DNA content in follicle cell populations containing these somatic clones show a 32n population not present in wild-type. However, amplification at specific loci (seen after stage 10B in wild-type) occurs normally in these enlarge nuclei.
Somatic clones expressing dmScer\UAS.cZa (driven by Scer\GAL4Act5C.PP, in the wing disc) are significantly larger than controls. Sibling clones next to these clones are smaller than controls elsewhere. However the A-P compartment boundary protects cells in the posterior compartment from cell competition induced by dmScer\UAS.cZa in the anterior compartment. Wing discs with clones show an increased level of apoptosis. dmScer\UAS.cZa driven by Scer\GAL4dpp.blk1 in the wing disc, leads in apoptosis. When somatic clones expressing dmScer\UAS.cZa is driven by Scer\GAL4αTub84B.PC or Scer\GAL4dpp.blk1, wing discs and adult wings are the same size as controls. When dmScer\UAS.cZa is ubiquitously driven in the wing disc by Scer\GAL4αTub84B.PC wings overgrow. Mutant wings (and mutant wing discs) are about 20% larger than controls and weigh about 15% more than controls. When 'wild-type' clones are made in wings ubiquitously expressing dmScer\UAS.cZa, the clones usually die, however the wing discs and adult wings are reduced in size compared to dmScer\UAS.cZa expressing discs and wings with no clones.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP in clones of cells does not promote cell growth under starvation conditions, but does promote DNA replication. Animals expressing dmScer\UAS.cZa under the control of Scer\GAL4Adh.PF or Scer\GAL4en-e16E are not starvation sensitive.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP in wing disc cells results in an increase in cell size compared to controls. The proportion of cells in G1 is decreased compared to controls. Clones expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP are not visibly round, do not generate ectopic folds and do not bulge out of the epithelium.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4ey.PH has no effect on eye size.
Clones expressing both dmScer\UAS.cZa and Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP in clones in the imaginal discs have a similar cell size and cell cycle profiles as those of cells expressing dmScer\UAS.cZa alone under the control of Scer\GAL4Act5C.PP. The elongated doubling time of cells expressing Ras85DN17.Scer\UAS under the control of Scer\GAL4Act5C.PP is partially rescued by co-expression of dmScer\UAS.cZa.
When expression is driven by Scer\GAL4Act5C.PP or Scer\GAL4en-e16E in clones in the wing disc cell size is increased. The increase in cell size is not accompanied by overgrowth of the wing, indicating that patterning mechanisms are not altered. Cell cycle phasing, but not cell cycle rate is altered. When expression is driven by Scer\GAL4C96 ectopic S phases occur in the ZNC of the wing disc, showing a block in cell cycle arrest. This block affects only the G1 arrest, G2 arrest proceeds normally. The non-arrested cells also show a block in their growth arrest.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Scer\GAL4GMR.PU, MycUAS.cZa has eye phenotype, enhanceable by ago4/ago[+]
NOT Enhanced by
Suppressed by
Statement
Reference
Scer\GAL4GMR.PU, MycUAS.cZa has eye phenotype, suppressible by pufA459
Scer\GAL4GMR.PU, ago1, MycUAS.cZa has eye phenotype, suppressible by pufA459
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
MycUAS.cZa/Scer\GAL4ey.PH is a suppressor of eye phenotype of Lfee
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
The reduced size of eyes and wings observed in adult flies expressing Larp4BGS9719 under the control of Scer\GAL4ey.3.5.Exel or Scer\GAL4sd.PU, respectively, along with the decreased cell size of larval fat body cells (with the Scer\GAL4ppl.PP driver) is suppressed by co-expression of MycScer\UAS.cZa.
The increased wing posterior compartment area induced by the expression of MycScer\UAS.cZa under the control of Scer\GAL4en-e16E is suppressed by the co-expression of nclbNIG.6751R.
The presence of Df(3L)banΔ1 partially suppresses the increased nucleolar size seen in secondary neuroblasts in larval brains expressing MycScer\UAS.cZa under the control of Scer\GAL4insc-Mz1407. Co-expression of both bansponge.Scer\UAS.T:Disc\RFP and MycScer\UAS.cZa fails to suppress the ectopic neuroblast formation and increased neuroblast numbers seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407. Co-expression of both MycScer\UAS.cZa and CG8184HMS01605 (but not MycScer\UAS.cZa alone) partially rescues the type II neuroblast loss phenotype seen in larval brains expressing numbScer\UAS.cOa under the control of Scer\GAL4insc-Mz1407.
Expression of MycScer\UAS.cZa in host midgut tissue only, under the control of Scer\GAL4GSG2326, fails to suppress the overgrowth of ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones or the reduced growth of wild type clones.
The co-expression of MycScer\UAS.cZa partially suppresses the decreased wing size of individuals expressing two copies of CNBPGD7370 under the control of Scer\GAL4nub.PU, and reared at 25[o[C temperature.
Co-expression of both dmScer\UAS.cZa and mir-308Scer\UAS.cDa under the control of Scer\GAL4da.G32 results in an increased proportion of the animals surviving to 96 hours after egg deposition compared to expression of either construct alone.
Expression of Sin3AKK100700 enhances the overproliferation and cell migration phenotypes seen when dmScer\UAS.cZa is expressed under the control of Scer\GAL4ptc-559.1.
Co-expression of dmScer\UAS.cZa partially suppresses the small wing phenotype caused by expression of brkScer\UAS.cDa under the control of Scer\GAL4nub-AC-62.
The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv is not suppressed if they are also co-expressing dmScer\UAS.cZa.
Co-expression of either MarfmiRNA.cUa.Scer\UAS or dmScer\UAS.cZa partially rescues the loss of type II neuroblasts seen in animals expressing NGD144 under the control of Scer\GAL4insc-Mz1407, while simultaneous co-expression of both MarfmiRNA.cUa.Scer\UAS and dmScer\UAS.cZa together fully rescues the phenotype.
Atg17d130 completely suppresses the dmScer\UAS.cZa-induced overgrowth of fat cells.
Co-expression of dmScer\UAS.cZa in subperineurial glia, with dupGD13203, both under the control of Scer\GAL4moody.PS, can restore normal cell size, polyploidy and accelerate the G1/S progression to wild-type levels.
Co-expression of vitoGD10890 reduces the size of Scer\GAL4Act5C.PI-indcued dmScer\UAS.cZa clones to control levels. This is the consequence of partial reversion of the dmScer\UAS.cZa-induced cell size increase together with a decrease in the number of cells per clone. Co-expression of vitoGD10890 considerably attenuates the increased cell size seen in Scer\GAL4ptc-559.1, dmScer\UAS.cZa salivary glands. This is a result of significant reductions in cytoplasmic and nuclear volumes. The nucleoli display a segregated organization, with a peripheral granular region surrounding an internal fibrillar area.
Co-expression of dmScer\UAS.cZa and ykiS168A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4repo.PU results in substantial increase in the number of glial cells in the larval eye disc and brain, greater than when either is expressed alone. Ethyl deoxyuridine (EdU) staining shows that there is increased glial cell proliferation in the cortex compared to controls, whereas this is not seen in the individual mutants.
The increased ommatidial size caused by expression of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF is enhanced by co-expression of HcfScer\UAS.T:Zzzz\FLAG. Co-expression of HcfScer\UAS.T:Zzzz\FLAG enhances the overgrowth seen in wing disc clones expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP. The wing defects caused by expression of dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 are partially suppressed by co-expression of either HcfGD4353 or HcfdsRNA.IR-3.Scer\UAS. Co-expression of either HcfdsRNA.IR-2.Scer\UAS or HcfdsRNA.IR-3.Scer\UAS strongly suppresses the excessive endoreplication seen in clones in the larval salivary glands expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP. The wing defects caused by expression of dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 are suppressed by co-expression of either ash2GD1374 or PcafGD11218.
The tissue and cell size defects caused by Scer\GAL4en-e16E>Dcr-1GD11429 in adult wings are largely suppressed when dmScer\UAS.cZa is co-expressed. Expression of dmScer\UAS.cZa under the control of Scer\GAL4αTub84B.PL in Dcr-1Q1147X mutant somatic clones completely suppresses cell loss, but clone size is not completely restored.
Expression of dmScer\UAS.cZa in ykiB5 wing disc clones (under the control of Scer\GAL4tub.PU) fails to rescue the growth defect of ykiB5 mutant clones. However, despite the small size of the clones, cell and nucleolar size are increased when dmScer\UAS.cZa is expressed in ykiB5 wing discs. Co-expression of dmScer\UAS.cZa and thV85M.Scer\UAS in wing disc clones (under the control of Scer\GAL4tub.PU) in ykiB5 mutant animals fails to rescue the growth defect of ykiB5 mutant clones, and growth rates are similar to when thV85M.Scer\UAS is expressed alone in ykiB5 mutant discs. Co-expression of dmScer\UAS.cZa and banEP3622 in wing disc clones (under the control of Scer\GAL4tub.PU) in ykiB5 mutant animals fails to rescue the growth defect of ykiB5 mutant clones, and growth rates are similar to when thV85M.Scer\UAS is expressed alone in ykiB5 mutant discs. However, despite the small size of the clones, cell and nucleolar size are increased when dmScer\UAS.cZa and banEP3622 are expressed in ykiB5 wing discs.
dmScer\UAS.cZa overexpression clones (under the control of Scer\GAL4hh.PU) found in the posterior of the wing disc strongly enhance the proliferative activity of ftG-rv, ds36D and exe1 mutant cells. In addition these cells are larger in dmScer\UAS.cZa clones than in a wild-type background.
Larvae co-expressing both dmScer\UAS.cZa and Su(z)2d01221 under the control of Scer\GAL4da.G32 hatch and grow normally.
Co-expression of btl::EgfrScer\UAS.T:λ\cI-DD and dmScer\UAS.cZa under the control of Scer\GAL4repo.PU induces glial neoplasia in third instar larval brains. These glia have very compact cell bodies relative to wild type glia.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4ey.PH in a Lfee background significantly restores eye size. Co-expression of dmScer\UAS.cZa suppresses the reduction in eye size caused by expression of TorWT.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey.PH.
The increased apoptosis that is seen in wing discs expressing dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 is not reduced by p535A-1-4/p5311-1B-1. The increased apoptosis that is seen in wing discs expressing dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 is suppressed by Df(3L)G870/+. The increased apoptosis that is seen in wing discs expressing dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 is reduced by Df(3L)H99.
Co-expression of MaxdsRNA.Scer\UAS.IR does not suppress the eye apoptosis phenotype caused by expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF. The increase in ommatidial size caused by expression of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of MaxdsRNA.Scer\UAS.IR. Co-expression of MntΔSID.Scer\UAS suppresses the increase in ommatidial size caused by expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF, but does not suppress apoptosis, resulting in a small and rough eye phenotype. Co-expression of MntΔZIP.Scer\UAS has no affect on the eye phenotypes caused by expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF. The eye phenotypes (apoptosis and increased ommatidial size) caused by expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF are not qualitatively altered by either Mnt1/Y or Mnt2/Y.
Co-expression of MaxdsRNA.Scer\UAS.IR does not suppress the eye apoptosis phenotype caused by expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF. The increase in ommatidial size caused by expression of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of MaxdsRNA.Scer\UAS.IR.
Expression of RpL8dsRNA.Scer\UAS partially suppresses the increase in eye size seen when dmScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF.
Co-expression of lidScer\UAS.P\T.cSa dramatically enhances the rough eye phenotype caused by expression of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF. Co-expression of lidJmjC.mut.Scer\UAS.P\T enhances the rough eye phenotype caused by expression of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF.
When dmScer\UAS.cZa is expressed clonally using the MARCM GAL repressor/activator system in wing disc cells that are RpI135k16513 mutant, the cell size effects of dmScer\UAS.cZa overexpression are significantly diminished. Cell cycle effects of dmScer\UAS.cZa overexpression are partially reversed in RpI135k16513 homozygous mutant cells.
The increased eye size and enlarged ommatidia of dmScer\UAS.cZa; Scer\GAL4GMR.PF flies is enhanced by pUf68EP3058/+. The resulting ommatidia are disorganised. The reduced thickness of bands of s-phase and mitotic cells posterior to the furrow in dmScer\UAS.cZa; Scer\GAL4GMR.PF eye discs is largely suppressed by pUf68EP3058/+.
The compensatory elongation of G2 phase seen in cells expressing dmScer\UAS.cZa driven by Scer\GAL4Act5C.PP is not as pronounced in a Dpa1/Dpa2 background.
Cells expressing dmScer\UAS.cZa driven by Scer\GAL4Act5C.PP in a Dpa1/Dpa2 background, the compensatory elongation of the G2 phase, is not as pronounced seen in dmScer\UAS.cZa expressing cells.
The amount of cell death seen in cells not expressing dmScer\UAS.cZa (in wing disc somatic clones driven by Scer\GAL4Act5C.PP) is reduced by about 15%. The presence of Df(3L)H99/+ or Df(3L)X14/+ in wing discs with dmScer\UAS.cZa expressing clones (driven by Scer\GAL4dpp.blk1) does not cause a growth disadvantage in cells neighbouring the clones. Neutral clones are similar in size whether near or far from the dmScer\UAS.cZa expressing cells. The anterior compartment in these discs is also significantly larger than normal. The presence of hepr75/+ in wing discs with dmScer\UAS.cZa expressing clones (driven by Scer\GAL4dpp.blk1) only reduces the cell death induced by cell competition by 30%. Wing discs are normal in size.
Expression of dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PI in clones in the eye disc which are homozygous for scrib1 increases the amount of mutant tissue compared to scrib1 single mutant clones, but the double mutant clones do not show metastatic behaviour.
The reduced wing phenotype caused by expression of TorTED.Scer\UAS under the control of Scer\GAL4Bx-MS1096 is partially suppressed by dmScer\UAS.cZa.
When dmScer\UAS.cZa (driven by Scer\GAL4mf9) is added to Df(2L)Prl/prd4, (partially rescued by a weakly expressing prdmf9 line), the accessory gland phenotype is reduced, and male fertility is restored. When dmScer\UAS.cZa (driven by Scer\GAL4prd.3.1) is added to Df(2L)Prl/prd4, however, no rescue is seen.
Expression of both Tsc1Scer\UAS.cTa and gigScer\UAS.cTa in the eye under the control of Scer\GAL4ey.PH results in a much smaller eye than normal. Coexpression of dmScer\UAS.cZa suppresses the phenotype.
When dmScer\UAS.cZa and stgScer\UAS.cNa are co-expressed via Scer\GAL4Act5C.PP in clones in the wing disc the cells show a 76% reduction in G2 cells (compared to dmScer\UAS.cZa alone). There is an increase of cells in G1 from 5% (dmScer\UAS.cZa alone) to 20%. Doubling time of cells was shortened from 12.9 to 10.6 hours. G1/S progression is promoted and the S and G2 phases of the cell cycle elongated. When dmScer\UAS.cZa and stgScer\UAS.cNa are co-expressed via Scer\GAL4Act5C.PP cells are smaller than for dmScer\UAS.cZa, dmScer\UAS.cZa alone, though the area of the dmScer\UAS.cZa, stgScer\UAS.cNa, Scer\GAL4Act5C.PP clones is larger than that of the dmScer\UAS.cZa, dmScer\UAS.cZa clones, because of reduced doubling time couple with reduced time between cell divisions.
Xenogenetic Interactions
Statement
Reference
Flies co-expressing dmScer\UAS.cZa and BacA\p35Scer\UAS.cHa at 25[o]C under the control of Scer\GAL4Act.PU and Scer\GAL80ts.αTub84B have a longer median lifespan than flies expressing dmScer\UAS.cZa alone.
Expression of BacA\p35Scer\UAS.cHa in wing disc clones expressing dmScer\UAS.cZa under the control of Scer\GAL4Act5C.PP results in large rounded cells 72 hours after clone induction. These enlarged cells are removed from the apical surface of the wind disc epithelium and extruded basally. Some mutant cells undergo apoptosis.
The increased apoptosis that is seen in larval eye discs expressing three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of BacA\p35Scer\UAS.cHa. Co-expression of BacA\p35Scer\UAS.cHa and three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in eyes and ommatidia that are significantly larger than eyes expressing either dmScer\UAS.cZa or BacA\p35Scer\UAS.cHa alone.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the reduction in pigment cells and disturbance of the internal architecture of the adult eye seen in animals by expression of three copies of dmScer\UAS.cZa under the control of Scer\GAL4GMR.PF.
Expression of dmScer\UAS.cZa partially suppresses the growth phenotypes seen in the wings and bristles of flies expressing Hsap\TGIF2LXScer\UAS.T:Hsap\MYC under the control of Scer\GAL4en-e16E or Scer\GAL4sca-537.4 respectively.
Expression of Hsap\RPL8DN.Scer\UAS partially suppresses the increase in eye size seen when dmScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF.
Complementation and Rescue Data
Comments
Expression of dmScer\UAS.cZa under the control of Scer\GAL4dm-PG45 fails to rescue the lethality of dmPG45 males (at either 18, 22 or 26[o]C).
When expression is driven by Scer\GAL4sca-109-68 or Scer\GAL4C-765, dmScer\UAS.cZa rescues the bristle area defect, cell area reduction, wing width and length reductions of dmP0 and the bristle area defect of dmP1 (rescue of wing phenotypes for dmP1 was not determined). When expression is driven by Scer\GAL4en-e16E the growth disadvantage of dmP0 clones in the wing disc is rescued.
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Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
MycScer\UAS.cZa
MycUAS.cZa
dmScer\UAS.cZa
dmUAS.cZa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Zaffran
Secondary FlyBase IDs
    References (84)