Allele Dmel\bsk^Scer\UAS.cBa

General Information
Symbol	Dmel\bsk^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Boutros	FlyBase ID	FBal0093136
Feature type	allele	Associated gene	Dmel\bsk

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Boutros et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of bsk cDNA is governed by Scer\UAS regulatory sequences. (Boutros et al., 1998)
Carried in construct	P{UAS-bsk.B} (Cha et al., 2005, Sanyal et al., 2002, Lee et al., 2003, Hsiao et al., 2001, Tran and Berg, 2003, Weber et al., 2000, McEwen et al., 2000, Boutros et al., 1998, Wang et al., 2003, Boettner et al., 2003, Cha et al., 2003, Singh et al., 2006, Chang and Neufeld, 2009, Chang and Neufeld, 2009, Lee et al., 2006, Xue et al., 2007, Tripura et al., 2011, Gonda et al., 2012, Mathew et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant, with Scer\GAL4^arm.PS (Wang et al., 2003) planar polarity defective, with Scer\GAL4^hs.2sev (Weber et al., 2000)
Phenotype Manifest In
	embryonic/larval fat body \| somatic clone, with Scer\GAL4^Act.PU (Chang and Neufeld, 2009) eye, with Scer\GAL4^ey.PH (Singh et al., 2006) eye, with Scer\GAL4^hs.2sev (Weber et al., 2000) ommatidium, with Scer\GAL4^hs.2sev (Weber et al., 2000) peripodial epithelium, with Scer\GAL4^{Ubx-lac1-Gal4} (Tripura et al., 2011) ventral furrow, with Scer\GAL4^twi.PG (Mathew et al., 2011)
Detailed Description
	Statement Reference Expression of bsk[Scer\UAS.cBa] under the control of Scer\GAL4[twi.PG] results in delayed or defective ventral furrow formation in a proportion of embryos. (Mathew et al., 2011) Expression of bsk[Scer\UAS.cBa] under the control of Scer\GAL4[Ubx-lac1-Gal4] results in transformation of posterior peripodial membrane cells of the wing disc from a hexagonal shape into an elongated shape. (Tripura et al., 2011) Expression of bsk[Scer\UAS.cBa] under the control of Scer\GAL4[Act] induces autophagy in clones in the larval fat body. (Chang and Neufeld, 2009) Expression of bsk[Scer\UAS.cBa] in the wing, under the control of Scer\GAL4[Bx-MS1096] results in mild or undetectable phenotypic changes. (Lee et al., 2006) Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH results in eyes that are smaller than wild type. (Singh et al., 2006) Overexpression of bsk^Scer\UAS.cBa, under the regulation of Scer\GAL4^ap-md544 has no effect on thorax closure. (Cha et al., 2005) Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF does not affect eye development. (Cha et al., 2003) Flies expressing bsk^Scer\UAS.cBa, under the control of Scer\GAL4^arm.PS, show a decreased sensitivity to paraquat compared to wild-type flies. (Wang et al., 2003) Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 has no effect on EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction. (Sanyal et al., 2002) When expression is driven by Scer\GAL4^hs.2sev, flies have rough eyes showing polarity phenotypes. Ommatidia are incorrectly rotated and sometimes exhibit the wrong chiral form. (Weber et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference bsk^Scer\UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, by^Scer\UAS.cLa (Lee et al., 2003)
Suppressor of
Statement Reference bsk^Scer\UAS.cBa/Scer\GAL4^tub.PU is a suppressor of oxidative stress response defective \| recessive phenotype of CYLD^mr4 (Xue et al., 2007) bsk^Scer\UAS.cBa/Scer\GAL4^tub.PU is a suppressor of short lived \| recessive phenotype of CYLD^mr4 (Xue et al., 2007) bsk^Scer\UAS.cBa is a suppressor of neuroanatomy defective phenotype of Scer\GAL4^elav-C155, puc^Scer\UAS.cMa (Sanyal et al., 2002) bsk^Scer\UAS.cBa is a suppressor of neurophysiology defective phenotype of Scer\GAL4^elav-C155, puc^Scer\UAS.cMa (Sanyal et al., 2002) Scer\GAL4^ptc-559.1/bsk^Scer\UAS.cBa is a suppressor \| partially of lethal \| embryonic stage phenotype of cno² (Boettner et al., 2003)
NOT Suppressor of
Statement Reference bsk^Scer\UAS.cBa, Scer\GAL4^ptc-559.1 is a non-suppressor of lethal \| embryonic stage phenotype of R^{N17.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^ptc-559.1 (Boettner et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference bsk^Scer\UAS.cBa has eye phenotype, enhanceable by L²/L[+] (Singh et al., 2006)
NOT suppressed by
Statement Reference Scer\GAL4^Act.PU, bsk^Scer\UAS.cBa has embryonic/larval fat body \| somatic clone phenotype, non-suppressible by Atg13^Δ81/Atg13^Δ81 (Chang and Neufeld, 2009)
Enhancer of
Statement Reference bsk^Scer\UAS.cBa, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Traf4^EP578 (Cha et al., 2003) bsk^Scer\UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of wing blade phenotype of Scer\GAL4^Bx-MS1096, lkb1^Scer\UAS.cLa (Lee et al., 2006) bsk^Scer\UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, by^Scer\UAS.cLa (Lee et al., 2003) bsk^Scer\UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, lkb1^Scer\UAS.cLa (Lee et al., 2006) bsk^Scer\UAS.cBa/Scer\GAL4^ey.PH is an enhancer of eye \| ventral phenotype of L² (Singh et al., 2006) bsk^Scer\UAS.cBa/Scer\GAL4^ey.PH is an enhancer of eye disc \| ventral phenotype of L² (Singh et al., 2006)
NOT Enhancer of
Statement Reference bsk^Scer\UAS.cBa/Scer\GAL4^c415 is a non-enhancer of dorsal appendage phenotype of bwk⁰⁸⁴⁸²/bwk¹⁵¹ (Tran and Berg, 2003) bsk^Scer\UAS.cBa/Scer\GAL4^dpp.PH is a non-enhancer of wing phenotype of Scer\GAL4^dpp.PH, eya^Scer\UAS.cHa (Hsiao et al., 2001)
Suppressor of
Statement Reference bsk^Scer\UAS.cBa/Scer\GAL4^{arm.T:Hsim\VP16} is a suppressor \| partially of embryonic epidermis \| ventral phenotype of arm³ (McEwen et al., 2000) bsk^Scer\UAS.cBa is a suppressor of bouton phenotype of Scer\GAL4^elav-C155, puc^Scer\UAS.cMa (Sanyal et al., 2002) bsk^Scer\UAS.cBa is a suppressor of neuromuscular junction \| larval stage phenotype of Scer\GAL4^elav-C155, puc^Scer\UAS.cMa (Sanyal et al., 2002) Scer\GAL4^ap-md544/bsk^Scer\UAS.cBa is a suppressor of adult thorax phenotype of Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} (Cha et al., 2005) Scer\GAL4^ap-md544/bsk^Scer\UAS.cBa is a suppressor of mesothoracic cleft \| ectopic phenotype of Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} (Cha et al., 2005) Scer\GAL4^ptc-559.1/bsk^Scer\UAS.cBa is a suppressor \| partially of dorsal closure embryo phenotype of cno² (Boettner et al., 2003)
NOT Suppressor of
Statement Reference bsk^Scer\UAS.cBa, Scer\GAL4^ptc-559.1 is a non-suppressor of dorsal closure embryo phenotype of R^{N17.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^ptc-559.1 (Boettner et al., 2003) bsk^Scer\UAS.cBa/Scer\GAL4^{arm.T:Hsim\VP16} is a non-suppressor of embryonic epidermis \| dorsal phenotype of arm³ (McEwen et al., 2000) bsk^Scer\UAS.cBa/Scer\GAL4^c415 is a non-suppressor of dorsal appendage phenotype of bwk⁰⁸⁴⁸²/bwk¹⁵¹ (Tran and Berg, 2003) bsk^Scer\UAS.cBa/Scer\GAL4^dpp.blk1 is a non-suppressor of eye \| ectopic phenotype of Scer\GAL4^dpp.blk1, eya^Scer\UAS.cHa (Hsiao et al., 2001) bsk^Scer\UAS.cBa/Scer\GAL4^dpp.PH is a non-suppressor of wing phenotype of Scer\GAL4^dpp.PH, eya^Scer\UAS.cHa (Hsiao et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference The autophagy that is induced by expression of bsk[Scer\UAS.cBa] under the control of Scer\GAL4[Act] in clones in the larval fat body still occurs in an Atg13[Δ81] homozygous background. (Chang and Neufeld, 2009) Expression of bsk[Scer\UAS.cBa] under the control of Scer\GAL4[tub.PU] rescues both the shortened lifespan increased sensitivity to paraquat which is seen in CYLD[mr4] mutant adults (animals homozygous for Df(2L)CYLD[mr4] and carrying P{LX107} to rescue the function of CG13138 and nmd). (Xue et al., 2007) Simultaneous expression of bsk[Scer\UAS.cBa] with lkb1[Scer\UAS.cLa] (both under the control of Scer\GAL4[Bx-MS1096] strongly enhances the lkb1[Scer\UAS.cLa] phenotype, resulting in severe disruption in the structure and size of the wing blades. (Lee et al., 2006) Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH enhances the loss-of-ventral-eye phenotype to near complete loss in 39% of L²/+ flies. (Singh et al., 2006) The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) is suppressed by co-expression of bsk^Scer\UAS.cBa. (Cha et al., 2005) Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ptc-559.1 results in a significant but partial rescue of the dorsal closure defects seen in cno² embryos. Co-expression of bsk^Scer\UAS.cBa does not rescue the dorsal closure defects seen in embryos expressing R^{N17.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1. (Boettner et al., 2003) Coexpression of bsk^Scer\UAS.cBa with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, leads to an increase in the disturbance of the ommatidial array in the compound eye in comparison to the eye phenotype resulting from one copy overexpression of Traf1^EP578. Coexpression of Traf2^EP1516 with either bsk^Scer\UAS.cBa, under the control of Scer\GAL4^GMR.PF, does not affect the development of eyes. (Cha et al., 2003) The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is not significantly modified by expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^c415. (Tran and Berg, 2003) The proportion of eya^Scer\UAS.cHa; Scer\GAL4^dpp.PH flies with blistered wings (76%) is relatively unaffected by the presence of bsk^Scer\UAS.cBa (82%), as is the proprotion with only mild wing defects 24% compared to 18%. (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case). (Hsiao et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	9310 w^*; P{UAS-bsk.B}2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	bsk^Scer\UAS.cBa bsk^UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Boutros (Boutros et al., 1998)
Secondary FlyBase IDs

References ( 19 )
Research paper	Gonda et al., 2012, PLoS ONE 7(7): e42369 Drosophila Heat Shock Response Requires the JNK Pathway and Phosphorylation of Mixed Lineage Kinase at a Conserved Serine-Proline Motif. [FBrf0219015] Mathew et al., 2011, Mol. Cell. Biol. 31(24): 4978--4993 Role for traf4 in polarizing adherens junctions as a prerequisite for efficient cell shape changes. [FBrf0216703] Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Regulation and activity of JNK signaling in the wing disc peripodial membrane during adult morphogenesis in Drosophila. [FBrf0216296] Chang and Neufeld, 2009, Mol. Biol. Cell 20(7): 2004--2014 An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation. [FBrf0207848] Xue et al., 2007, Dev. Cell 13(3): 446--454 Tumor suppressor CYLD regulates JNK-Induced cell death in Drosophila. [FBrf0200559] Lee et al., 2006, Cell Death Differ. 13(7): 1110--1122 JNK pathway mediates apoptotic cell death induced by tumor suppressor LKB1 in Drosophila. [FBrf0194576] Singh et al., 2006, Development 133(23): 4771--4781 Lobe and Serrate are required for cell survival during early eye development in Drosophila. [FBrf0192707] Cha et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(29): 10345--10350 Parkin negatively regulates JNK pathway in the dopaminergic neurons of Drosophila. [FBrf0188253] Boettner et al., 2003, Genetics 165(1): 159--169 The AF-6 homolog canoe acts as a Rap1 effector during dorsal closure of the Drosophila embryo. [FBrf0162150] Cha et al., 2003, Mol. Cell. Biol. 23(22): 7982--7991 Discrete functions of TRAF1 and TRAF2 in Drosophila melanogaster mediated by c-Jun N-terminal kinase and NF-kappaB-dependent signaling pathways. [FBrf0167884] Lee et al., 2003, Development 130(17): 4001--4010 blistery encodes Drosophila tensin protein and interacts with integrin and the JNK signaling pathway during wing development. [FBrf0160724] Tran and Berg, 2003, Development 130(25): 6273--6282 bullwinkle and shark regulate dorsal-appendage morphogenesis in Drosophila oogenesis. [FBrf0167514] Wang et al., 2003, Dev. Cell 5(5): 811--816 JNK signaling confers tolerance to oxidative stress and extends lifespan in Drosophila. [FBrf0167481] Hsiao et al., 2001, Dev. Cell 1(1): 51--61 Eyes absent mediates cross-talk between retinal determination genes and the receptor tyrosine kinase signaling pathway. [FBrf0141456] McEwen et al., 2000, Development 127(16): 3607--3617 The canonical Wg and JNK signaling cascades collaborate to promote both dorsal closure and ventral patterning. [FBrf0128573] Weber et al., 2000, Development 127(16): 3619--3629 Jun mediates Frizzled-induced R3/R4 cell fate distinction and planar polarity determination in the Drosophila eye. [FBrf0128678] Boutros et al., 1998, Cell 94(1): 109--118 Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. [FBrf0103240]
Supplementary material	Chang and Neufeld, 2009, Mol. Biol. Cell 20(7): Supplemental Data. [FBrf0208115]
Letter	Sanyal et al., 2002, Nature 416(6883): 870--874 AP-1 functions upstream of CREB to control synaptic plasticity in Drosophila. [FBrf0147170]

Allele Dmel\bskScer\UAS.cBa

Allele Dmel\bsk^Scer\UAS.cBa