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General Information
Symbol
Dmel\Nint.SH.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0093233
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Nintra, UAS-Nintra, UAS-Notchintra, Nintra
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The extracellular and transmembrane domains of N are deleted but the DNA coding for the intracellular domain is kept intact.

UAS regulatory sequences drive expression of a constitutively active form of N.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

chemosensory sensory organ & wing vein L1 & glial cell, with Scer\GAL4hs.PB

chemosensory sensory organ & wing vein L3 & glial cell, with Scer\GAL4hs.PB

dorsal mesothoracic disc & filamentous actin | somatic clone, with Scer\GAL4Act5C.PI

mechanosensory sensory organ & wing vein L1 & glial cell, with Scer\GAL4hs.PB

mechanosensory sensory organ & wing vein L3 & glial cell, with Scer\GAL4hs.PB

tarsal segment 4 & joint, with Scer\GAL4ap-md544

tormogen cell & antennal segment 3 | conditional ts, with Scer\GAL4sca-P309

tormogen cell & antennal segment 3 | ectopic | conditional ts, with Scer\GAL4sca-P309

trichogen cell & antennal segment 3 | conditional ts, with Scer\GAL4sca-P309

trichogen cell & antennal segment 3 | ectopic | conditional ts, with Scer\GAL4sca-P309

Detailed Description
Statement
Reference

Expression of Nint.SH.Scer\UAS under the control of Scer\GAL4insc.PU in embryos leads to a significant reduction in thoracic and abdominal neuroblast daughter cell proliferation at stage 12 (but not stage 14), but no significant difference in thoracic or abdominal neuroblast proliferation at stage 12 or 14, as compared to controls.

Clones of Nint.SH.Scer\UAS in the wing disc, driven by Scer\GAL4Act5C.PI, have no autonomous effect on F-actin. Occasionally, upregulation of F-actin is observed at the clone edges consistent with N ligand expression.

When Nint.SH.Scer\UAS, Scer\GAL4sca-P309 animals are kept at 16oC until 10 h APF, and then shifted to 28oC (to activate Scer\GAL4sca-P309 in secondary progenitors of external sensory organs), adult antenna show a variety of defects. Multiple sockets are seen, and sensilla with two shafts arising from a socket.

When Nint.SH.Scer\UAS is driven by Scer\GAL4how-24B in the mesoderm differentiation of the visceral mesoderm is disrupted.

When expression is driven by Scer\GAL4hs.PB at the time of the birth of the sensory organ precursor cells all L3 glia and most L1 glia are absent. Transformations affecting sensory organ precursor IIb (into IIa), the glial precursor (into IIIb) and the neuron (into a thecogen cell) are observed. When expression is driven in non-gliogenic sensory organ lineages, partial or total transformation of neurons in thecogen sheath cells is observed. The strongest defect is a sensory organs of 6 cells, four being thecogen cells.

Expression of Nint.SH.Scer\UAS under the control of Scer\GAL4klu-G410 results in lethality. Expression of Nint.SH.Scer\UAS under the control of Scer\GAL4ap-md544 results in a hyper-jointed fourth tarsal segment; double ball joints with reversed polarity are formed. In addition, the interjoint region is reduced.

Embryos carrying Nint.SH.Scer\UAS driven by Scer\GAL4da.G32 or Scer\GAL4how-24B appear to have no muscle founder cells, and fail to differentiate muscle fibres. Embryos carrying Nint.SH.Scer\UAS driven by Scer\GAL4da.G32 have no PNS structures.

Flies carrying Nint.SH.Scer\UAS expressed under the control of Scer\GAL4sca-P309 (reared at 18oC throughout development and raised to 25oC at the beginning of pupation) show duplication of the tormogen at the expense of the trichogen cell, leading to double sockets. Three-socket and four-socket phenotypes are also seen.

When expression is driven by Scer\GAL4pnr-MD237 or Scer\GAL4da.G32 no effect on dorsal closure is seen.

Rough eye phenotype.

Scer\GAL4da.G32 mediated expression of Nint.SH.Scer\UAS blocks neural development completely.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The addition of l(1)scScer\UAS.cHa and daScer\UAS.cGa does not rescue the mesoderm phenotype of Nint.SH.Scer\UAS driven by Scer\GAL4da.G32. Co-expression of HScer\UAS.cGb and Nint.SH.Scer\UAS driven by Scer\GAL4da.G32 or Scer\GAL4how-24B produces embryos with fewer muscle founder cells than wild-type, but more than seen in embryos with Nint.SH.Scer\UAS alone (driven by Scer\GAL4da.G32 or Scer\GAL4how-24B) alone. Also well differentiated muscles are found, in contrast to Nint.SH.Scer\UAS alone. Co-expression of HScer\UAS.cGb and Nint.SH.Scer\UAS driven by Scer\GAL4da.G32 CNS and some PNS structures are seen to differentiate in contrast to Nint.SH.Scer\UAS alone (driven by Scer\GAL4da.G32).

The addition of l(1)scScer\UAS.cHa and daScer\UAS.cGa does not rescue the mesoderm phenotype of Nint.SH.Scer\UAS driven by Scer\GAL4da.G32.

Co-expression of HScer\UAS.cGb and Nint.SH.Scer\UAS driven by Scer\GAL4da.G32 or Scer\GAL4how-24B produces embryos with fewer muscle founder cells than wild-type, but more than seen in embryos with Nint.SH.Scer\UAS alone (driven by Scer\GAL4da.G32 or Scer\GAL4how-24B) alone. Also well differentiated muscles are found, in contrast to Nint.SH.Scer\UAS alone.

Co-expression of HScer\UAS.cGb and Nint.SH.Scer\UAS driven by Scer\GAL4da.G32 results in the differentiation of CNS and some PNS structures, in contrast to Nint.SH.Scer\UAS alone (driven by Scer\GAL4da.G32).

Scer\GAL4da.G32 mediated expression of Nint.SH.Scer\UAS blocks neural development completely, even when co-expressed with daScer\UAS.cGa and l(1)scScer\UAS.cHa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Rescue of the neurogenic phenotype is good in both the dorsal and ventral regions of the embryo. The dorsal closure defect is not significantly rescued.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

L. Seugnet M. Haenlin

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Secondary FlyBase IDs
    References (25)