Allele Dmel\Egfr::tor^act.Scer\UAS

General Information
Symbol	Dmel\Egfr::tor^act.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0093398
Feature type	allele	Associated gene	Dmel\Egfr::tor

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dominguez et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An activated form of Egfr is expressed under the control of Scer\UAS regulatory sequences. (Dominguez et al., 1998)
Carried in construct	P{UAS-tor⁴⁰²¹-EGFR} (Rohrbaugh et al., 2002, Lawrence et al., 2002, Gallio et al., 2004, Frankfort and Mardon, 2004, Almudi et al., 2009, Llimargas, 2000, Casci et al., 1999, Feldmann et al., 1999, Dominguez et al., 1998, Page, 2003, Parker, 2006, Brown et al., 2006, Mao and Freeman, 2009, Buchon et al., 2010, MacDougall et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	cell death defective \| somatic clone, with Scer\GAL4^{Myo31DF-NP0001}, Scer\GAL80^ts.αTub84B (Buchon et al., 2010) decreased cell death \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) increased cell size \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) lethal \| heat sensitive, with Scer\GAL4^Bx-MS1096 (Feldmann et al., 1999) visible, with Scer\GAL4^Bx-MS1096 (Feldmann et al., 1999) visible, with Scer\GAL4^hs.2sev (Almudi et al., 2009)
Phenotype Manifest In
	adult gut, with Scer\GAL4^{Myo31DF-NP0001} (Buchon et al., 2010) adult gut, with Scer\GAL4^{Myo31DF-NP0001}, Scer\GAL80^ts.αTub84B (Buchon et al., 2010) dorsocentral bristle, with Scer\GAL4^C-765 (Mao and Freeman, 2009) embryonic segment, with Scer\GAL4^en-e16E (Parker, 2006) enterocyte \| somatic clone, with Scer\GAL4^unspecified (Buchon et al., 2010) eye, with Scer\GAL4^hs.2sev (Almudi et al., 2009) eye disc, with Scer\GAL4^Act5C.PI (Dominguez et al., 1998) ganglionic branch 1 \| embryonic stage, with Scer\GAL4^bs-23.26 (Gallio et al., 2004) midgut, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Buchon et al., 2010) morphogenetic furrow, with Scer\GAL4^Act5C.PI (Dominguez et al., 1998) morphogenetic furrow \| cell non-autonomous \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Frankfort and Mardon, 2004) morphogenetic furrow \| somatic clone, with Scer\GAL4^Act5C.PI (Brown et al., 2006) ommatidial precursor cluster \| somatic clone, with Scer\GAL4^Act5C.PI (Brown et al., 2006) photoreceptor \| cell autonomous \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Frankfort and Mardon, 2004) photoreceptor cell, with Scer\GAL4^Act5C.PI (Dominguez et al., 1998) photoreceptor cell R7 \| supernumerary, with Scer\GAL4^hs.2sev (Almudi et al., 2009) wing, with Scer\GAL4^Bx-MS1096 (Feldmann et al., 1999)
Detailed Description
	Statement Reference Overexpression of Egfr::tor[act.Scer\UAS] in intestinal stem cells (ISCs) under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B] is sufficient to induce proliferation along the gut in the absence of infection, although the resulting cells are abnormally shaped and do not appear as fully differentiated enterocytes. Expression of Egfr::tor[act.Scer\UAS] in enterocytes under the control of Scer\GAL4[Myo31DF-NP0001] results in a adult flies with guts two times shorter and three times wider than wild-type fly guts. Guts of flies overexpressing Egfr::tor[act.Scer\UAS] under the control of Scer\GAL4[Myo31DF-NP0001] and Scer\GAL80[ts.αTub84B] are shorter compared with guts wild-type flies 48 hours post-infection with Erwinia carotovora carotovora 15 (Ecc15). Guts of flies expressing Egfr::tor[act.Scer\UAS] in enterocytes under the control of Scer\GAL4[Myo31DF-NP0001] and Scer\GAL80[ts.αTub84B] do not elongate upon infection with Erwinia carotovora carotovora 15 (Ecc15). After a recovery phase, the guts are 40% shorter than their wild-type counterparts. Overexpression of Egfr::tor[act.Scer\UAS] in enterocytes under the control of Scer\GAL4[Myo31DF-NP0001] and Scer\GAL80[ts.αTub84B] induces hight levels of cell delamination in the absence of Erwinia carotovora carotovora 15 (Ecc15) infection. Clones of enterocytes overexpressing Egfr::tor[act.Scer\UAS] under the control of Scer\GAL4[Myo31DF-NP0001] and Scer\GAL80[ts.αTub84B] rapidly disappear from the epithelium compared with wild-type clones suggesting an increased rate of delamination. The detached cells in these guts differ from those in wild-type flies in that they do not undergo cell death, have normal nuclei and do not contain enlarged vacuoles. (Buchon et al., 2010) Expression of Egfr::tor[act.Scer\UAS] under the control of Scer\GAL4[hs.2sev] results in a rough eye phenotype. 20% of ommatidia have an extra R7 photoreceptor cell. (Almudi et al., 2009) Expression of Egfr::tor[act.Scer\UAS] in the adult notum under the control of Scer\GAL4[C-765] results in extra dorsocentral bristles. (Mao and Freeman, 2009) Clones that express Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^Act5C.PI form large, disorganised clusters in morphogenetic furrow. The cells of the furrow appear to be disrupted in these clones. (Brown et al., 2006) Expression of Egfr::tor^act.Scer\UAS, under the control of Scer\GAL4^en-e16E, in the posterior compartment cells if embryos leads to a reduction in the level of apoptosis of these cells, but has no effect on mitosis. Cells are of an increased size leading to an increase in compartment size of 18%. (Parker, 2006) In late 3rd instar eye discs, Egfr::tor^act.Scer\UAS; Scer\GAL4^Act5C.PI somatic clones exhibit ectopic neuronal differentiation, but photoreceptors in these clones fail to express R8 markers. When the clones fall anterior to the morphogenetic furrow, precocious neural differentiation occurs within the clone which also non-autonomously induces the formation of ectopic furrows. (Frankfort and Mardon, 2004) Ganglionic branch 1s turn prematurely before reaching the midline in Egfr::tor^act.Scer\UAS; Scer\GAL4^bs-23.26 embryos. (Gallio et al., 2004) Clones of Egfr::tor^act.Scer\UAS (driven by Scer\GAL4^αTub84B.PC) in the adult abdomen do not show any consistent alterations of normal polarity. (Lawrence et al., 2002) Expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^btl.PS does not confer dorsal trunk or visceral branch features to other tracheal branches. (Llimargas, 2000) Flies expressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 do not eclose at 25^oC, at 18^oC the flies eclose but have abnormal wings. (Feldmann et al., 1999) Clones in the eye disc expressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^Act5C.PI posterior to the morphogenetic furrow show extra photoreceptor cell recruitment. Ectopic morphogenetic furrow formation is seen. (Dominguez et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement Reference Egfr::tor^act.Scer\UAS, Scer\GAL4^hs.2sev has visible phenotype, non-suppressible by Socs36E^{Scer\UAS.P\T.cCa}, Scer\GAL4^hs.2sev (Almudi et al., 2009)
Suppressor of
Statement Reference Egfr::tor^act.Scer\UAS, Scer\GAL4^en-e16E is a suppressor of increased cell death \| embryonic stage phenotype of CycE^Scer\UAS.cLa, Scer\GAL4^en-e16E (Parker, 2006)
Phenotype Manifest In
Suppressed by
Statement Reference Egfr::tor^act.Scer\UAS, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has morphogenetic furrow \| ectopic \| somatic clone \| cell non-autonomous phenotype, suppressible by sens^Scer\UAS.cNa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Frankfort and Mardon, 2004) Egfr::tor^act.Scer\UAS, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI has photoreceptor \| ectopic \| somatic clone \| cell autonomous phenotype, suppressible \| partially by sens^Scer\UAS.cNa, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Frankfort and Mardon, 2004) Egfr::tor^act.Scer\UAS, Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B has midgut phenotype, suppressible \| partially by dome^{ΔTMCYT.Scer\UAS}, Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Buchon et al., 2010) Egfr::tor^act.Scer\UAS, Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B has midgut phenotype, suppressible \| partially by Socs36E^Scer\UAS.cCa, Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Buchon et al., 2010) Egfr::tor^act.Scer\UAS, Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B has midgut phenotype, suppressible \| partially by Stat92E^NIG.cUa, Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Buchon et al., 2010)
NOT suppressed by
Statement Reference Egfr::tor^act.Scer\UAS, Scer\GAL4^hs.2sev has eye phenotype, non-suppressible by Socs36E^{Scer\UAS.P\T.cCa}, Scer\GAL4^hs.2sev (Almudi et al., 2009) Egfr::tor^act.Scer\UAS, Scer\GAL4^hs.2sev has photoreceptor cell R7 \| supernumerary phenotype, non-suppressible by Socs36E^{Scer\UAS.P\T.cCa}, Scer\GAL4^hs.2sev (Almudi et al., 2009)
Suppressor of
Statement Reference Egfr::tor^act.Scer\UAS, Scer\GAL4^en-e16E is a suppressor of embryonic segment \| posterior compartment phenotype of CycE^Scer\UAS.cLa, Scer\GAL4^en-e16E (Parker, 2006) Egfr::tor^act.Scer\UAS, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is a suppressor of ommatidial precursor cluster \| somatic clone phenotype of ato¹ (Brown et al., 2006) Egfr::tor^act.Scer\UAS/Scer\GAL4¹⁴⁰⁷ is a suppressor of embryonic central brain phenotype of sim² (Page, 2003)
NOT Suppressor of
Statement Reference Egfr::tor^act.Scer\UAS/Scer\GAL4^αTub84B.PL is a non-suppressor of ommatidial precursor cluster \| somatic clone phenotype of pnt^Δ88 (Brown et al., 2006)
Other
Statement Reference ato³/Df(3R)p13, Egfr::tor^act.Scer\UAS, Scer\GAL4^bi-omb-Gal4 has eye disc & neuron phenotype (Dominguez et al., 1998) Egfr::tor^act.Scer\UAS, Scer\GAL4^bi-omb-Gal4, ato³ has eye disc & neuron phenotype (Dominguez et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference The mitotic index in the gut of flies overexpressing Egfr::tor[act.Scer\UAS] is decreased but not abolished when dome[ΔTMCYT.Scer\UAS] is co-expressed under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B]. The mitotic index in the gut of flies overexpressing Egfr::tor[act.Scer\UAS] is decreased but not abolished when Stat92E[NIG.cUa] is co-expressed under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B]. The mitotic index in the gut of flies overexpressing Egfr::tor[act.Scer\UAS] is decreased but not abolished when Socs36E[Scer\UAS.cCa] is co-expressed under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B]. (Buchon et al., 2010) The rough eye and extra R7 photoreceptor cell phenotypes caused by expression of Egfr::tor[act.Scer\UAS] under the control of Scer\GAL4[hs.2sev] are not suppressed by co-expression of Socs36E[Scer\UAS.P\T.cCa]. (Almudi et al., 2009) Expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^αTub84B.PL does not rescue the lack of arcs and rosettes in the morphogenetic furrow of eye discs with pnt^Δ88 Minute clones. Expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^GMR.PF partially rescues the lack of cluster formation in the morphogenetic furrow of ato¹ eye disc clones. (Brown et al., 2006) Coexpression of Egfr::tor^act.Scer\UAS and CycE^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E, blocks CycE^Scer\UAS.cLa-induced apoptosis in the embryonic posterior compartment cells. There is a reduction in compartment size, similar to that seen when only Ras85D^V12.Scer\UAS is expressed, and cells are of a size similar to that seen when only CycE^Scer\UAS.cLa is expressed. (Parker, 2006) Precocious and ectopic neuronal differentiation in Egfr::tor^act.Scer\UAS; Scer\GAL4^Act5C.PI somatic clones in the eye discs is largely suppressed by sens^Scer\UAS.cNa, as is ectopic morphogenetic furrow formation. (Frankfort and Mardon, 2004) The addition of Egfr::tor^act.Scer\UAS (driven by Scer\GAL4¹⁴⁰⁷) to sim² suppresses the sim² embryonic brain phenotype. In 55% of embryos, restoration of the midbrain, including ganglia and axons tracts is seen. (Page, 2003) Neuronal differentiation is seen in ato³/Df(3R)p13 eye discs expressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^bi-omb-Gal4. (Dominguez et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Egfr::tor^act.Scer\UAS Egfr::tor^act.UAS
Name Synonym	Activated Egfr (Lawrence et al., 2002)
Secondary FlyBase IDs

References ( 15 )
Research paper	Buchon et al., 2010, BMC Biol. 8: 152 Drosophila EGFR pathway coordinates stem cell proliferation and gut remodeling following infection. [FBrf0212840] Almudi et al., 2009, Dev. Biol. 326(1): 212--223 SOCS36E specifically interferes with Sevenless signaling during Drosophila eye development. [FBrf0207068] Mao and Freeman, 2009, Development 136(3): 473--481 Fasciclin 2, the Drosophila orthologue of neural cell-adhesion molecule, inhibits EGF receptor signalling. [FBrf0206540] Brown et al., 2006, Dev. Biol. 300(2): 710--721 Epithelial cell adhesion in the developing Drosophila retina is regulated by Atonal and the EGF receptor pathway. [FBrf0192868] Parker, 2006, Curr. Biol. 16(20): 2058--2065 Control of compartment size by an EGF ligand from neighboring cells. [FBrf0193620] Frankfort and Mardon, 2004, Development 131(3): 563--570 Senseless represses nuclear transduction of Egfr pathway activation. [FBrf0167533] Gallio et al., 2004, Development 131(15): 3605--3614 Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline. [FBrf0179216] MacDougall et al., 2004, Mol. Cell. Biol. 24(2): 796--808 Targeted expression of the class II phosphoinositide 3-kinase in Drosophila melanogaster reveals lipid kinase-dependent effects on patterning and interactions with receptor signaling pathways. [FBrf0167897] Page, 2003, Curr. Biol. 13(6): 474--482 A function for EGF receptor signaling in expanding the developing brain in Drosophila. [FBrf0158813] Lawrence et al., 2002, Development 129(11): 2749--2760 Towards a model of the organisation of planar polarity and pattern in the Drosophila abdomen. [FBrf0148964] Rohrbaugh et al., 2002, Curr. Biol. 12(7): 576--581 Notch Activation of yan Expression Is Antagonized by RTK/Pointed Signaling in the Drosophila Eye. [FBrf0146938] Llimargas, 2000, Development 127(20): 4407--4417 wingless and its signalling pathway have common and separable functions during tracheal development. [FBrf0129949] Casci et al., 1999, Cell 96(5): 655--665 Sprouty, an intracellular inhibitor of Ras signaling. [FBrf0107641] Feldmann et al., 1999, Mol. Cell. Biol. 19(3): 1928--1937 Control of growth and differentiation by Drosophila RasGAP, a homolog of p120 ras-GTPase-activating protein. [FBrf0106478] Dominguez et al., 1998, Curr. Biol. 8(19): 1039--1048 Multiple functions of the EGF receptor in Drosophila eye development. [FBrf0104744]

Allele Dmel\Egfr::toract.Scer\UAS

Allele Dmel\Egfr::tor^act.Scer\UAS