Expression of salmScer\UAS.cKa under the control of Scer\GAL41151 restores the normal muscle fibre structure in dorsal longitudinal indirect flight muscles in vgnull mutants. Fibrillar transformation of leg muscles is not observed as a result of salmScer\UAS.cKa expression.
At early stages (15), the VL muscles show no significant migration or attachment defects in vgnull; rhea1 double mutant embryos. However, at later stages (16+) several muscles, most prevalently VL1 can be seen detaching from their normal location. This muscle detachment phenotype appears to be solely due to a lack of vg activity as it can be rescued through expression of vgScer\UAS.T:Ivir\HA1 in muscle cells.
Expression of pucScer\UAS.cMa (which inhibits cell death) in vgnull clones results in clone survival in the prospective wing blade of the larval wing disc. Clone survival depends on the position along the proximal-distal axis of growth, clones being recovered at a higher frequency and larger size in the proximal territories of the vg expression domain. These clones tend to be extruded during larval stages and fail to appear in the adult wing.
The severity of wing defects caused by expression of sdScer\UAS.cVa under the control of Scer\GAL4vg.int2.1 is enhanced by vgnull/+; in the double mutant flies 93% have an "extreme" phenotype (thorax flight appendages are completely missing and no hinge structures are present) and 7% have a "severe" phenotype (wing blade growth is severely impaired).
Expression of dapScer\UAS.cLa along the dorsal/ventral boundary of the wing under the control of Scer\GAL4vg.int2.1 in a vgnull/+ mutant background results in nicks at the wing tips. There is a significant decrease in mitotic cells along the dorsal/ventral boundary.
Co-expression of vgScer\UAS.cZa in flies expressing dapScer\UAS.cLa at the dorsal/ventral wing boundary under the control of Scer\GAL4vg.int2.1 in a vgnull/+ mutant background rescues wing morphology, and mitotic activity of cells is restored in this region.
Expression of thScer\UAS.cLa at the dorsal/ventral wing disc boundary under the control of Scer\GAL4vg.int2.1 rescues the strong wing margin phenotype observed in vgnull/+ flies grown on aminopterin (0.5mg/kg).
sd[ETX4]/Y males display an enhanced wing phenotype in a vgnull/+ background.
Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 does not wing blade growth in vgnull flies and only a slight increase in wing hinge growth is seen.