FlyBase Allele Report: Dmel\vg[null]

General Information

Symbol

Dmel\vg^null

Species

D. melanogaster

Name

FlyBase ID

FBal0093753

Feature type

allele

Associated gene

Dmel\vg

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Paumard-Rigal et al., 1998)

Progenitor genotype

Cytology

Description

Deletion of the eight exons of vg.

(Paumard-Rigal et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

vg^null mutant dorsal longitudinal indirect flight muscles are reduced in number and show an abnormal tubular fibre phenotype. The mutant flies are viable and can walk. Their leg muscles are normal.

(Schönbauer et al., 2011)

In homozygous vg^null embryos VL2 muscles are absent in at least 30% of segments. This muscle loss seems to be VL2 cell specific because VL1 muscles are present in all segments. However, the loss of vg does not seem to block initial formation of adhesions between two differentiating muscles, because, as in stage 16 wild-type embryos, homozygous vg^null exhibit tight adhesions between pairs of VL muscles between segments.

The VL1 muscle is initially specified correctly in all segments in vg^null embryos.

(Deng et al., 2010)

The waves of contraction seen in newly hatched larvae are much slower than normal in sd⁹ hemizygotes, taking approximately 2 times as long to pass from posterior tip to anterior tip compared to wild type.

The VL2 muscle is often missing in mutant embryos (there are no detectable defects in other muscles).

(Deng et al., 2009)

vg^null clones in the prospective wing blade of the larval wing disc die quickly in either a wild type or Minute genetic background.

(Baena-Lopez and Garcia-Bellido, 2006)

Adult thoraces of vg^null flies show muscle degeneration.

(Bernard et al., 2006)

The dorsal/ventral compartment boundary is only slightly disturbed in vg^null/vg^83b27-R wing discs.

(Koelzer and Klein, 2006)

Mutants show no growth of wing blade tissue, although some hinge structures are still present.

(Legent et al., 2006)

In vg^null mutant wing discs fewer cells are observed in S-phase compared to controls, and discs are reduced in size. There is also a significant decrease in cells in the M phase.

vg^null/+ flies display nicks at the wing tip when raised on a low dose of aminopterin (0.5mg/kg).

High levels of cell death are observed at the dorsal/ventral boundary in vg^null/+ wing discs from animals raised on aminopterin (2mg/kg).

(Delanoue et al., 2004)

In homozygous vg^null flies, some dorsal medial muscles are present but no coxal tergal remotor muscles (AKA dorsoventral muscles). The level of degeneration increases in old flies. However, the site of muscle attachment to the epidermis appears normal. Dorsal medial muscle configuration is normal in 48-h APF mutant pupae, indicating that splitting occurs normally and that degeneration of these muscles occurs during late pupal stages and in the adult. Examination of degenerating muscle fibers in mutant adults shows a complete disorganization of myofibrils and nuclei showing morphological signs of apoptosis.

(Bernard et al., 2003)

vg^null/vg^83b27 adults show an almost complete lack of wing structures.

(Delanoue et al., 2002)

Significant cell death is not seen in the third larval instar wing disc (similar to wild type).

Cell death is induced in the wing disc by expression vg^tr.Scer\UAS under the control of Scer\GAL4^ptc-559.1 in a vg^null/vg^null background.

(van de Bor et al., 1999)

Homozygotes show extreme reduction of the wings. Flies heterozygous for vg^null in a sd^ETX4 background do not show such a significant reduction in the wing as flies heterozygous for vg^79d5 in a sd^ETX4 or sd¹ background. The formation of ectopic wing-like outgrowths caused by expression of vg^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 is more strongly reduced in a vg^null homozygous background than in a vg^null heterozygous background.

(Paumard-Rigal et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

vg^null has visible | dominant | conditional phenotype, enhanceable by Df(3R)P-10/+

(Delanoue et al., 2004)

NOT Enhanced by

Statement

Reference

vg^null has visible | dominant | conditional phenotype, non-enhanceable by E2f[+]/E2f1⁹¹

(Delanoue et al., 2004)

Suppressed by

Statement

Reference

dap^UAS.cLa, vg[+]/vg^null has decreased occurrence of cell division | larval stage phenotype, suppressible by vg^UAS.cZa/Scer\GAL4^vg.int2.1

(Delanoue et al., 2004)

vg^null has decreased occurrence of cell division | larval stage phenotype, suppressible by Scer\GAL4^ptc-559.1/E2f1^UAS.cNa/BacA\p35^UAS.cHa

(Delanoue et al., 2004)

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has visible phenotype, suppressible by vg^UAS.cZa/Scer\GAL4^vg.int2.1

(Delanoue et al., 2004)

vg^null/vg^83b27 has visible phenotype, suppressible | partially by ap^UAS.cMa/Scer\GAL4^vg.boundary

(Delanoue et al., 2002)

Enhancer of

Statement

Reference

vg[+]/vg^null is an enhancer of visible phenotype of Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

vg[+]/vg^null is an enhancer of visible phenotype of Scer\GAL4^vg.int2.1, dap^UAS.cLa

(Legent et al., 2006)

vg[+]/vg^null is an enhancer of visible phenotype of sd^ETX4

(Delanoue et al., 2004)

vg[+]/vg^null is an enhancer of visible phenotype of Bx³

(Delanoue et al., 2002)

Suppressor of

Statement

Reference

vg[+]/vg^null is a suppressor | partially of visible phenotype of BacA\p35^UAS.cHa, Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^vg.int2.1

(Legent et al., 2006)

Other

Statement

Reference

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has visible phenotype

(Delanoue et al., 2004)

dap^UAS.cLa, vg[+]/vg^null has decreased occurrence of cell division | larval stage phenotype

(Delanoue et al., 2004)

Scer\GAL4^vg.PU, dap^UAS.cLa, vg[+]/vg^null has visible phenotype

(van de Bor et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

vg^null has wing | conditional phenotype, enhanceable by Df(3R)P-10/+

(Delanoue et al., 2004)

NOT Enhanced by

Statement

Reference

vg^null has wing | conditional phenotype, non-enhanceable by E2f[+]/E2f1⁹¹

(Delanoue et al., 2004)

Suppressed by

Statement

Reference

vg^null has dorsal longitudinal indirect flight muscle cell phenotype, suppressible | partially by salr^UAS.cSa/Scer\GAL4¹¹⁵¹

(Schönbauer et al., 2011)

vg^null has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by salm^UAS.cKa/Scer\GAL4¹¹⁵¹

(Schönbauer et al., 2011)

vg^null has wing disc | somatic clone phenotype, suppressible | partially | somatic clone by puc^UAS.cMa/Scer\GAL4^αTub84B.PL

(Baena-Lopez and Garcia-Bellido, 2006)

vg^null has indirect flight muscle cell phenotype, suppressible by Scer\GAL4¹¹⁵¹/fng^UAS.cKa

(Bernard et al., 2006)

vg^null has wing phenotype, suppressible by Scer\GAL4^ptc-559.1/E2f1^UAS.cNa/BacA\p35^UAS.cHa

(Delanoue et al., 2004)

vg^null has wing | conditional phenotype, suppressible by Diap1^UAS.cLa/Scer\GAL4^vg.int2.1

(Delanoue et al., 2004)

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has wing phenotype, suppressible by vg^UAS.cZa/Scer\GAL4^vg.int2.1

(Delanoue et al., 2004)

vg^null/vg^83b27 has wing phenotype, suppressible | partially by ap^UAS.cMa/Scer\GAL4^vg.boundary

(Delanoue et al., 2002)

Enhancer of

Statement

Reference

vg[+]/vg^null is an enhancer of wing phenotype of Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

vg[+]/vg^null is an enhancer of wing blade phenotype of Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

vg[+]/vg^null is an enhancer of wing hinge phenotype of Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

vg[+]/vg^null is an enhancer of wing phenotype of Scer\GAL4^vg.int2.1, dap^UAS.cLa

(Legent et al., 2006)

vg[+]/vg^null is an enhancer of wing margin phenotype of sd^ETX4

(Delanoue et al., 2004)

vg[+]/vg^null is an enhancer of wing phenotype of Bx³

(Delanoue et al., 2002)

Suppressor of

Statement

Reference

vg[+]/vg^null is a suppressor | partially of wing phenotype of BacA\p35^UAS.cHa, Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^vg.int2.1

(Legent et al., 2006)

Other

Statement

Reference

rhea¹, vg^null has muscle cell of A1-7 ventral longitudinal muscle 1 phenotype

(Deng et al., 2010)

rhea¹, vg^null has muscle attachment site phenotype

(Deng et al., 2010)

Scer\GAL4^vg.int2.1, dap^UAS.cLa, vg[+]/vg^null has wing phenotype

(Delanoue et al., 2004)

Scer\GAL4^vg.PU, dap^UAS.cLa, vg[+]/vg^null has wing phenotype

(van de Bor et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of salm^Scer\UAS.cKa under the control of Scer\GAL4¹¹⁵¹ restores the normal muscle fibre structure in dorsal longitudinal indirect flight muscles in vg^null mutants. Fibrillar transformation of leg muscles is not observed as a result of salm^Scer\UAS.cKa expression.

Overexpression of salr^Scer\UAS.cSa under the control of Scer\GAL4¹¹⁵¹ results in some suppression of the vg^null-mutant dorsal longitudinal indirect flight muscle phenotype.

(Schönbauer et al., 2011)

At early stages (15), the VL muscles show no significant migration or attachment defects in vg^null; rhea¹ double mutant embryos. However, at later stages (16+) several muscles, most prevalently VL1 can be seen detaching from their normal location. This muscle detachment phenotype appears to be solely due to a lack of vg activity as it can be rescued through expression of vg^{Scer\UAS.T:Ivir\HA1} in muscle cells.

The direction of VL1 muscle migration is unaffected in vg^null; rhea¹ double mutants.

The development of tendon cells is not affected in vg^null; rhea¹ double mutants.

(Deng et al., 2010)

Expression of puc^Scer\UAS.cMa (which inhibits cell death) in vg^null clones results in clone survival in the prospective wing blade of the larval wing disc. Clone survival depends on the position along the proximal-distal axis of growth, clones being recovered at a higher frequency and larger size in the proximal territories of the vg expression domain. These clones tend to be extruded during larval stages and fail to appear in the adult wing.

(Baena-Lopez and Garcia-Bellido, 2006)

Adult thoraces of vg^null, Scer\GAL4¹¹⁵¹, fng^Scer\UAS.cKa flies show a significant rescue of muscle degeneration compared to thoraces of vg^null flies.

(Bernard et al., 2006)

The severity of wing defects caused by expression of sd^Scer\UAS.cVa under the control of Scer\GAL4^vg.int2.1 is enhanced by vg^null/+; in the double mutant flies 93% have an "extreme" phenotype (thorax flight appendages are completely missing and no hinge structures are present) and 7% have a "severe" phenotype (wing blade growth is severely impaired).

The severity of the wing nicking phenotype caused by expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^vg.int2.1 is greatly enhanced by vg^null/+.

(Legent et al., 2006)

Expression of dap^Scer\UAS.cLa along the dorsal/ventral boundary of the wing under the control of Scer\GAL4^vg.int2.1 in a vg^null/+ mutant background results in nicks at the wing tips. There is a significant decrease in mitotic cells along the dorsal/ventral boundary.

Co-expression of vg^Scer\UAS.cZa in flies expressing dap^Scer\UAS.cLa at the dorsal/ventral wing boundary under the control of Scer\GAL4^vg.int2.1 in a vg^null/+ mutant background rescues wing morphology, and mitotic activity of cells is restored in this region.

vg^null/+, Df(3R)P-10/+ double heterozygous flies raised on a low dose of aminopterin (0.5mg/kg) display a strong notched-wing phenotype.

vg^null/+, E2f⁹¹/+ flies display small notches at the wing tip when raised on a low dose of aminopterin (0.5mg/kg).

Expression of th^Scer\UAS.cLa at the dorsal/ventral wing disc boundary under the control of Scer\GAL4^vg.int2.1 rescues the strong wing margin phenotype observed in vg^null/+ flies grown on aminopterin (0.5mg/kg).

sd[ETX4]/Y males display an enhanced wing phenotype in a vg^null/+ background.

(Delanoue et al., 2004)

Expression of ap^Scer\UAS.cMa under the control of Scer\GAL4^vg.boundary partially rescues the loss of wing tissue seen in vg^null/vg^83b27 flies.

The scalloped wing phenotype seen in Bx³ homozygotes is enhanced by vg^null/+.

(Delanoue et al., 2002)

Expression of dap^Scer\UAS.cLa under the control of Scer\GAL4^vg.PU in a vg^null/+ background results in a mutant wing phenotype.

(van de Bor et al., 1999)

Xenogenetic Interactions

Statement

Reference

Co-expression of E2f^Scer\UAS.cNa, Dp^Scer\UAS.cDa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^vg.int2.1 results in a wing nicking phenotype, which is partially suppressed by vg^null/+.

Co-expression of E2f^Scer\UAS.cNa, Dp^Scer\UAS.cDa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^vg.int2.1 does not wing blade growth in vg^null flies and only a slight increase in wing hinge growth is seen.

(Legent et al., 2006)

Expression of E2f^Scer\UAS.cNa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 in vg^null mutant discs results in an increase in the number of cells in S-phase.

(Delanoue et al., 2004)

Complementation and Rescue Data

Rescued by

vg^null is rescued by vg^UAS.cZa

(Delanoue et al., 2004)

Partially rescued by

vg^null is partially rescued by vg^UAS.cZa/Scer\GAL4^ap-md544

(Bernard et al., 2003)

Comments

Expression of vg^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 in vg^null mutant discs increases the number of cells in S-phase and results in overgrowth of the disc along the anterior/posterior axis. An increase in cells in M-phase is observed.

(Delanoue et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

vg⁰

(Schönbauer et al., 2011)

vg^null

(Pimmett et al., 2017, Requena et al., 2017, Deng et al., 2010, Bernard et al., 2009, Deng et al., 2009, Baena-Lopez and Garcia-Bellido, 2006, Bernard et al., 2006, Gajewski et al., 2006, Koelzer and Klein, 2006, Legent et al., 2006, Delanoue et al., 2004, Delanoue et al., 2002, van de Bor et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (18)

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