|Feature type||allele||Associated gene||Dmel\lush|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
lush1 Or67d-expressing olfactory sensory neurons show severely decreased spontaneous activity compared to control neurons.
T1 neurons from lush mutants do not respond to 11-cis vaccenyl acetate. Behavioural attraction of lush mutant flies to wild-type males is significantly reduced compared to control flies. When female flies are used as bait, male flies show an increased attraction compared to females, but there is no difference in this attraction between wild-type and lush mutants. Substitution of male flies for 1% 11-cis vaccenyl acetate does not affect wild-type attraction. However lush mutants are completely defective for attraction to 11-cis vaccenyl acetate. T1 neurons in lush mutants exhibit a greater than 400-fold reduction in spontaneous activity. Instead of one spike per second, the spontaneous activity in T1 neurons from lush mutants is approximately 1 spike every 430 seconds. No difference in spontaneous activity is observed in the T2 neurons of wild-type and lush mutants, despite an equivalent loss of lush protein from these sensilla. No difference in mating latency, inappropriate mating behaviour, or mating stages is found in lush mutants compared to wild-type. T2B neurons are defective for inhibition by high concentrations of ethanol and butanol in lush mutants. In wild-type T2B sensilla, at least one and usually several olfactory neurons within the sensillum reduce firing rates upon stimulation by concentrated butanol or ethanol. lush mutant T2B neurons do not show this normal inhibitory response to these odorants and continue firing at prestimulus rates. Low concentrations of these odorants have no effect on the activity of these neurons.
Mutant flies respond normally to both high and low concentrations of ethanol and ethyl acetate (they are attracted to low concentrations and repulsed by high concentrations) in a T-maze assay. Mutant flies show an abnormal response to benzaldehyde (BA) in a T-maze assay; they do not show the wild-type attraction to low concentrations of BA, while they are repulsed by high concentrations, as in wild type. The amplitude of neural response in the mushroom bodies in response to BA is similar in mutant and wild-type flies, even at the concentration of BA at which the behavioural defect is seen. Mutant flies show similar mushroom body activity patterns upon repetitive delivery of the same odour, similar to wild-type flies. However, the concentration-dependency of spatial distribution patterns which is seen in wild-type mushroom bodies in response to BA is abolished in mutant flies. As in wild type, ethyl acetate (EA) evokes concentration dependent patterns of distributed neural activity. Patterns evoked by different odours, as shown by comparisons among citral, octanol, EA and BA show similar odour specificity as in wild type.
Homozygous flies show an apparent increased attraction to ethanol, propanol and butanol in an olfactory trap assay compared to control flies. This abnormal response is specific to high odorant concentrations; attraction of flies to low concentrations of ethanol is similar to wild-type. Third instar larvae show a normal response to high concentrations of ethanol. The olfactory behaviour response to butanone, acetone, ethyl acetate, isoamyl acetate, acetic acid, benzaldehyde, methanol, isopropanol, pentanol and yeast is not significantly different from wild-type. Homozygous flies are defective for the avoidance behavioural response to concentrated ethanol which is present in wild-type flies. The electrical responses of ethanol-sensitive neurons to ethanol appear normal.
|NOT suppressed by|
|NOT Suppressor of|
|Phenotype Manifest In|
|NOT suppressed by|
Double mutants defective for lush Snmp[Z0429] exhibit high spontaneous activity, indicating that Snmp functions downstream of lush in cVA signaling.
|Complementation & Rescue Data|
The presence of lush[+tKa] rescues lush mutant T1 neuron responsiveness to 11-cis vaccenyl acetate. lush mutant flies transformed with a copy of lush[+tKa] respond to 11-cis vaccenyl acetate and male flies in behavioural attraction assays. Normal spontaneous activity rates are restored to T1 neurons upon introduction of lush[+tKa]. lush mutants expressing lush[+tKa] have inhibitory responses to concentrated alcohol restored.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 5 )|
|Secondary FlyBase IDs|
|References ( 10 )|