Allele Dmel\cnk^{hs.sev.T:Zzzz\FLAG}

General Information
Symbol	Dmel\cnk^{hs.sev.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0093975
Feature type	allele	Associated gene	Dmel\cnk

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Therrien et al., 1998) in vitro construct - regulatory fusion (Therrien et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length cnk cDNA tagged with T:Zzzz\FLAG is expressed from the dual sev enhancer Hsp70 promoter. (Therrien et al., 1998)
Carried in construct	P{sE-cnk.FLAG} (Douziech et al., 2003, Therrien et al., 1999, Laberge et al., 2005, Therrien et al., 1998)
Tagged with	T:Zzzz\FLAG (Therrien et al., 1998, Therrien et al., 1999, Douziech et al., 2003, Laberge et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	visible (Therrien et al., 1999, Therrien et al., 1998)
Phenotype Manifest In
	eye (Therrien et al., 1999, Therrien et al., 1998) photoreceptor (Therrien et al., 1998) photoreceptor cell R7 (Therrien et al., 1998)
Detailed Description
	Statement Reference Mutant eyes appear wild-type - ommatidial organisation is normal. (Laberge et al., 2005) Eye surface is roughened. (Therrien et al., 1999) Mild rough eye phenotype, with the anterior portion of the eye being more disorganised. R7 and outer photoreceptors are missing. (Therrien et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement Reference cnk^{hs.sev.T:Zzzz\FLAG} is a non-enhancer of visible phenotype of Ras85D^{V12.C40.Scer\UAS}, Scer\GAL4^hs.2sev (Therrien et al., 1999) cnk^{hs.sev.T:Zzzz\FLAG} is a non-enhancer of visible phenotype of Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^sev.EP (Therrien et al., 1999)
Phenotype Manifest In
Enhancer of
Statement Reference cnk^{hs.sev.T:Zzzz\FLAG} is an enhancer of eye phenotype of Ras85D^{V12.G37.Scer\UAS}, Scer\GAL4^hs.2sev (Therrien et al., 1999) cnk^{hs.sev.T:Zzzz\FLAG} is an enhancer of eye phenotype of Ras85D^V12.sev (Therrien et al., 1999, Therrien et al., 1998)
Suppressor of
Statement Reference cnk^{hs.sev.T:Zzzz\FLAG} is a suppressor \| partially of photoreceptor cell R7 \| supernumerary phenotype of Ras85D^V12.sev (Douziech et al., 2003) cnk^{hs.sev.T:Zzzz\FLAG} is a suppressor of eye phenotype of phl::tor^13D.hs.sev (Therrien et al., 1999, Therrien et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference The enhancement effect of Ras85D^{V12.G37.Scer\UAS} on cnk^{hs.sev.T:Zzzz\FLAG} contrasts sharply with the lack of enhancement of cnk^{hs.sev.T:Zzzz\FLAG} by Ras85D^{V12.S35.Scer\UAS} or Ras85D^{V12.C40.Scer\UAS}. (Therrien et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	cnk^{hs.sev.T:FLAG} cnk^{hs.sev.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Laberge et al., 2005, EMBO J. 24(3): 487--498 Src42 binding activity regulates Drosophila RAF by a novel CNK-dependent derepression mechanism. [FBrf0183948] Douziech et al., 2003, EMBO J. 22(19): 5068--5078 Bimodal regulation of RAF by CNK in Drosophila. [FBrf0167547] Therrien et al., 1999, Proc. Natl. Acad. Sci. U.S.A. 96(23): 13259--13263 Functional analysis of CNK in RAS signaling. [FBrf0112056] Therrien et al., 1998, Cell 95(3): 343--353 CNK, a RAF-binding multidomain protein required for RAS signaling. [FBrf0105423]

Allele Dmel\cnkhs.sev.T:Zzzz\FLAG

Allele Dmel\cnk^{hs.sev.T:Zzzz\FLAG}