FlyBase Allele Report: Dmel\hid[Ala5.GMR]

General Information

Symbol

Dmel\hid^Ala5.GMR

Species

D. melanogaster

Name

FlyBase ID

FBal0093995

Feature type

allele

Associated gene

Dmel\hid

Associated Insertion(s)

Carried in Construct

P{GMR-hid.Ala5}

Also Known As

GMR-hid^Ala5

Key Links

Transgenic product class

missense_variant

(Bergmann et al., 1998)

polypeptide_post_translational_processing_variant

(Bergmann et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Bergmann et al., 1998)

polypeptide_post_translational_processing_variant

(Bergmann et al., 1998)

Mutagen

in vitro construct

(Bergmann et al., 1998)

Progenitor genotype

Carried in construct

P{GMR-hid.Ala5}

Cytology

Description

Five out of five phosphorylation sites mutated. Amino acid replacement: S121A. Amino acid replacement: T148A. Amino acid replacement: T180A. Amino acid replacement: T228A. Amino acid replacement: S251A. Expression is governed by GMR regulatory sequences.

(Bergmann et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

hid

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Gafuik and Steller, 2011, Bergmann et al., 1998)

visible

(Schoenherr et al., 2012, Hilgers et al., 2010, Brennecke et al., 2003, Bergmann et al., 1998)

Phenotype Manifest In

eye

(Schoenherr et al., 2012, Hilgers et al., 2010, Brennecke et al., 2003, Bergmann et al., 1998)

Detailed Description

Statement

Reference

W^Ala5.GMR flies have a smaller eye than normal.

(Schoenherr et al., 2012)

W^Ala5.GMR flies exhibit a small eye phenotype due to increased apoptosis in the eye.

(Guan et al., 2007)

Mutant animals have small rough eyes.

(Brennecke et al., 2003)

Eye reduced. Phenotypes of W^GMR.PG, W^Ala3.GMR and W^Ala5.GMR are comparable. Some lines completely lack eye structures. Phenotype is slightly suppressed by hemizygosity at the endogenous W locus.

(Bergmann et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hid^Ala5.GMR has visible phenotype, enhanceable by mir-bft^Δ263a

(Hilgers et al., 2010)

hid^Ala5.GMR has visible phenotype, enhanceable by Df(3L)mir-ban^Δ1

(Brennecke et al., 2003)

Suppressed by

Statement

Reference

hid^Ala5.GMR has visible phenotype, suppressible by Ack^UAS.cSb/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, suppressible by aos[+]/aos^Δ7/Ack^UAS.cSb/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, suppressible by RasGAP1^mip-w+/Ack^UAS.cSb/Gap1[+]/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^GMR.PF

(Brennecke et al., 2003)

hid^Ala5.GMR has increased cell death phenotype, suppressible by sav³

(Tapon et al., 2002)

NOT suppressed by

Statement

Reference

hid^Ala5.GMR has visible phenotype, non-suppressible by aos[+]/aos^Δ7/Ack^K156A.UAS/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, non-suppressible by RasGAP1^mip-w+/Gap1[+]/Ack^K156A.UAS/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, non-suppressible by drk^KK109233/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, non-suppressible by RasGAP1^mip-w+/Gap1[+]

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, non-suppressible by aos[+]/aos^Δ7

(Schoenherr et al., 2012)

hid^Ala5.GMR has visible phenotype, non-suppressible by Ack^K156A.UAS/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has increased cell death phenotype, non-suppressible by Ras85D^R68Q

(Gafuik and Steller, 2011)

Other

Statement

Reference

Ack^UAS.cSb, Hsp83^GD1202, Scer\GAL4^GMR.PU, hid^Ala5.GMR has lethal phenotype

(Schoenherr et al., 2012)

Ack^UAS.cSb, Scer\GAL4^GMR.PU, hid^Ala5.GMR, yki^KK109756 has lethal phenotype

(Schoenherr et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

hid^Ala5.GMR has eye phenotype, enhanceable by mir-bft^Δ263a

(Hilgers et al., 2010)

hid^Ala5.GMR has eye phenotype, enhanceable by Df(3L)mir-ban^Δ1

(Brennecke et al., 2003)

Suppressed by

Statement

Reference

hid^Ala5.GMR has eye phenotype, suppressible by Ack^UAS.cSb/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, suppressible by aos[+]/aos^Δ7/Ack^UAS.cSb/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, suppressible by RasGAP1^mip-w+/Ack^UAS.cSb/Gap1[+]/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^GMR.PF

(Brennecke et al., 2003)

hid^Ala5.GMR has eye phenotype, suppressible by Diap1^GMR.PH

(Bergmann et al., 1998)

hid^Ala5.GMR has eye phenotype, suppressible by rl^Sem

(Bergmann et al., 1998)

NOT suppressed by

Statement

Reference

hid^Ala5.GMR has eye phenotype, non-suppressible by RasGAP1^mip-w+/Gap1[+]

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, non-suppressible by aos[+]/aos^Δ7

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, non-suppressible by Ack^K156A.UAS/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, non-suppressible by aos[+]/aos^Δ7/Ack^K156A.UAS/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, non-suppressible by RasGAP1^mip-w+/Gap1[+]/Ack^K156A.UAS/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has eye phenotype, non-suppressible by drk^KK109233/Scer\GAL4^GMR.PU

(Schoenherr et al., 2012)

hid^Ala5.GMR has phenotype, non-suppressible by Ras85D^V12.sev

(Bergmann et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for either Gap1^mip-w+ or aos^Δ7 does not suppress the small eye phenotype caused by W^Ala5.GMR.

Expression of Ack^{K156A.Scer\UAS} under the control of Scer\GAL4^GMR.PU (either alone, or in conjunction with either aos^Δ7/+ or Gap1^mip-w+/+) does not suppress the small eye phenotype caused by W^Ala5.GMR.

Expression of Ack^Scer\UAS.cSb under the control of Scer\GAL4^GMR.PU suppresses the small eye phenotype caused by W^Ala5.GMR. Further suppression is seen if the flies are also heterozygous for either aos^Δ7/+ or Gap1^mip-w+/+.

Co-expression of either Hsp26^KK106347 or Magi^GD10066 further reduces the size of the eye in a W^Ala5.GMR, Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU background.

Co-expression of drk^KK109233 does not modify eye size in animals expressing W^Ala5.GMR.

Co-expression of drk^KK109233 in a W^Ala5.GMR, Ack^Scer\UAS.cSb, Scer\GAL4^GMR.PU background results in flies with eyes that are greatly reduced in size.

(Schoenherr et al., 2012)

Ras85D^R68Q fails to suppress W^Ala5.GMR (which lacks all of the 5 predicted MAPK phosphorylation sites) induced cell death.

(Gafuik and Steller, 2011)

Expression of dco^Scer\UAS.cSa in the eye (under the control of Scer\GAL4^GMR.PF) strongly suppresses the pro-apoptotic activity of W^Ala5.GMR.

(Guan et al., 2007)

Eye size is largely restored by co-expression of ban^EP3622 (driven by Scer\GAL4^GMR.PF) with W^Ala5.GMR. The addition of Df(3L)ban^Δ1 to W^Ala5.GMR animals leads to a significant enhancement of the eye phenotype.

(Brennecke et al., 2003)

When sav³ clones are induced in the eye disc in W^Ala5.GMR mutants, the induction of apoptosis is reduced in a cell autonomous manner.

(Tapon et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

W^Ala5.GMR

hid^Ala5.GMR

Name Synonyms

Secondary FlyBase IDs

References (8)

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