FlyBase Allele Report: Scer\GAL4[17d]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^17d

General Information

Symbol

Scer\GAL4^17d

Species

S. cerevisiae

Name

FlyBase ID

FBal0094031

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

17D-GAL4, 17d, P{GAL4}17d

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Liu et al., 1999)

Progenitor genotype

Associated Insertion(s)

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

inner layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

outer layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

core layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

surface layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

intrinsic neuron | subset

(Kitamoto, 2002)

Kenyon cell | subset

(Liu et al., 1999)

adult median bundle

(Zars et al., 2000)

intrinsic neuron of adult mushroom body beta-lobe

(Kitamoto, 2002)

adult mushroom body beta-lobe

(Knight et al., 2010, Baker et al., 2007, Kitamoto, 2002, Zars et al., 2000)

intrinsic neuron of adult mushroom body alpha-lobe

(Kitamoto, 2002)

adult mushroom body alpha-lobe

(Knight et al., 2010, Baker et al., 2007, Kitamoto, 2002, Zars et al., 2000)

alpha/beta Kenyon cell | subset

(Buchanan and Davis, 2010)

adult pars intercerebralis | faint | restricted

(Knight et al., 2010)

adult mushroom body

(Kitamoto, 2002, Zars et al., 2000)

adult mushroom body | restricted

(Melzig et al., 1998)

mushroom body beta lobe core layer | intense

(Aso et al., 2009)

mushroom body alpha lobe core layer | intense

(Aso et al., 2009)

mushroom body beta lobe surface layer | faint

(Aso et al., 2009)

mushroom body alpha lobe surface layer | faint

(Aso et al., 2009)

adult tritocerebrum | faint

(Aso et al., 2009)

alpha/beta Kenyon cell

(Pauls et al., 2010)

adult mushroom body alpha-lobe

(Pauls et al., 2010)

adult mushroom body beta-lobe

(Pauls et al., 2010)

Additional Information

Statement

Reference

Drives expression in the alpha and beta lobes of the mushroom body.

(FlyBase Curators, 2017)

ScerGAL4^17d shows strong expression in the central core of mushroom body alpha/beta neurons.

(Buchanan and Davis, 2010)

ScerGAL4^17d is expressed in the mushroom body alpha and beta lobes and weakly in some cells of the pars intercerebralis.

(Knight et al., 2010)

Marker for

adult median bundle

(Zars et al., 2000)

adult mushroom body alpha-lobe

(Zars et al., 2000)

adult mushroom body beta-lobe

(Zars et al., 2000)

(Kitamoto, 2002)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(King et al., 2011)

abnormal courtship behavior, with fru^RNAi.M.UAS

(Manoli et al., 2005)

abnormal learning, with Ent2^GD953

(Knight et al., 2010)

abnormal locomotor rhythm, with Ctet\tetX^TNT-LC.UAS

(Martin et al., 1998)

abnormal memory, with Ctet\tetX^TNT-LC.UAS

(Liu et al., 1999)

abnormal memory | adult stage, with Dop2R^RNAi.UAS.cDa

(Scholz-Kornehl and Schwärzel, 2016)

abnormal memory | adult stage, with Inx5^JF02877

(Liu et al., 2016)

abnormal memory | adult stage, with Inx5^KK103391

(Liu et al., 2016)

abnormal memory | adult stage, with Inx6^GD3692

(Liu et al., 2016)

abnormal memory | adult stage, with Inx6^JF02168

(Liu et al., 2016)

abnormal memory | adult stage, with zpg^GD2338

(Liu et al., 2016)

abnormal memory | adult stage | conditional, with Npha\HR^{eNpHR2.0.UAS.cBa.YFP}

(Liu et al., 2016)

abnormal memory | adult stage | conditional, with Npha\HR^UAS.cLa.EYFP

(Liu et al., 2016)

abnormal sleep, with Pka-R1^{BDK.UAS.Tag:HA}

(Crocker et al., 2010)

abnormal thermotaxis, with Hsap\KCNJ2^UAS.EGFP

(Hong et al., 2008)

chemical resistant | adult stage, with Sirt1^KK109370

(Engel et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic stimulation (using Npha\HR^{e.UAS.cBa.YFP} or Npha\HR^UAS.cLa.EYFP with 593 nm (yellow) light activation) of Scer\GAL4^17d neurons significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls; this effect is not seen when expression is restricted to a smaller subset of cells using Scer\GAL80^Mef2.mb247.

Blocking the output (using shi^ts.UAS.cUa at 30[o]C or Ctet\TeTxLC^UAS.cUa) of Scer\GAL4^17d neurons does not affect visual short term memory following training with pain stimulus in adult flies, compared to controls.

(Liu et al., 2016)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^17d cells in adults does not significantly affect aversive 1-min visual or olfactory memory, compared to controls.

(Vogt et al., 2014)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^17d/Dop2R^UAS.cSa rescues Dop2R^Δ1

(Scholz-Kornehl and Schwärzel, 2016)

Scer\GAL4^GH298, Pde4^UAS.cCa, Scer\GAL4^17d rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Fails to rescue

Adcy1^UAS.cZa/Scer\GAL4^17d fails to rescue Adcy1^rut-1

(Scheunemann et al., 2012)

Scer\GAL4^17d, inc^f00285 fails to rescue inc^f00285

(Stavropoulos and Young, 2011)

Nf1^UAS.cBa/Scer\GAL4^17d fails to rescue Nf1^c00617

(Buchanan and Davis, 2010)

Nf1^UAS.cBa/Scer\GAL4^17d fails to rescue Nf1^P1

(Buchanan and Davis, 2010)

Dop1R1^UAS.cKa/Scer\GAL4^17d fails to rescue Dop1R1^f02676

(Kong et al., 2010)

Adcy1^UAS.cZa/Scer\GAL4^17d fails to rescue Adcy1^rut-2080

(Zars et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

51631

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

17d

(Kitamoto, 2002)

GAL4^17d

Scer\GAL4^17D

(Manoli et al., 2005)

Scer\GAL4^17d

Name Synonyms

Secondary FlyBase IDs

FBal0098827

References (49)

Report Sections