FlyBase Allele Report: Scer\GAL4[αTub84B.PP]

General Information

Symbol

Scer\GAL4^αTub84B.PP

Species

S. cerevisiae

Name

α-Tubulin at 84B promoter construct of Pignoni

FlyBase ID

FBal0094032

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-αTub84B(-FRT).P}

Also Known As

tub-gal4, tubGal4, tubulin-Gal4

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Katanaev et al., 2005, Dearborn and Kunes, 2004, Delon et al., 2003, Briscoe et al., 2001, Huang and Kunes, 1998, Huang et al., 1998, Pignoni, 1998.11.9)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Scer\GAL4^{FRT.Rnor\Cd2.αTub84B}

(Katanaev et al., 2005, Dearborn and Kunes, 2004, Delon et al., 2003, Campbell, 2002, Briscoe et al., 2001, Huang and Kunes, 1998, Huang et al., 1998, Pignoni, 1998.11.9)

Carried in construct

P{GAL4-αTub84B(-FRT).P}

Cytology

Nature of the lesion

Statement

Reference

The FRT cassette has been excised, juxtaposing the αTub84B promoter with sequence encoding a GAL4 driver.

(Pignoni, 1998.11.9)

Allele components

Product class / Tool use(s)

Regulatory region(s)

αTub84B

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Designed for use in mosaic analyses; used to drive gene of interest in Scer\FLP1-generated clones. The progenitor construct is P{GAL4-αTub84B(FRT.CD2).P}; see Scer\GAL4^{FRT.Rnor\Cd2.αTub84B} elsewhere in this table.

(FlyBase Curators, 2017)

Designed for use in mosaic analyses; used to drive gene of interest in FLP1-generated clones.

(Huang and Kunes, 1998)

Marker for

Reflects expression of

αTub84B

(Huang and Kunes, 1998)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased cell number | somatic clone, with ci^76.UAS

(Huang and Kunes, 1998)

decreased cell number | somatic clone, with Myc^GD1419

(Ziosi et al., 2010)

decreased cell number | somatic clone, with smo³

(Huang and Kunes, 1998)

hyperplasia | somatic clone, with yki^UAS.cHa

(Ziosi et al., 2010)

increased cell number | somatic clone, with dx^UAS.cMa

(Koelzer and Klein, 2006)

increased cell size | somatic clone, with Akt1^{UAS.Tag:HA,Tag:MyrPalm(Lck)}

(Tiefenböck et al., 2010)

increased cell size | somatic clone, with InR^UAS.cHa

(Tiefenböck et al., 2010, Baltzer et al., 2009)

increased cell size | somatic clone, with Myc^UAS.cSAa

(Baltzer et al., 2009)

increased cell size | somatic clone, with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Tiefenböck et al., 2010)

increased cell size | somatic clone, with yki^UAS.cHa

(Ziosi et al., 2010)

increased occurrence of cell division | larval stage | somatic clone, with dx^UAS.cMa

(Koelzer and Klein, 2006)

lethal - all die before end of larval stage, with ft^UAS.cMa

(Matakatsu and Blair, 2004)

neuroanatomy defective, with Ctet\tetX^TNT-LC.UAS

(Roy et al., 2007)

neuroanatomy defective, with DCTN1-p150^Δ.UAS

(Roy et al., 2007)

neuroanatomy defective, with EcR^{B1-ΔC655.W650A.UAS}

(Roy et al., 2007)

neuroanatomy defective, with Eph^DN.UAS

(Dearborn et al., 2002)

neuroanatomy defective, with Hsap\KCNJ2^UAS.EGFP

(Roy et al., 2007)

neuroanatomy defective, with shi^1.UAS

(Roy et al., 2007)

neuroanatomy defective | third instar larval stage, with Reph^UAS.cDa

(Dearborn et al., 2012)

planar polarity defective | cell non-autonomous | somatic clone, with fz^UAS.cSa

(Katanaev et al., 2005)

some die during larval stage, with ft^UAS.cMa

(Matakatsu and Blair, 2004)

visible, with dpp^UAS.cSa

(Kopp et al., 1999)

visible | somatic clone, with wg^UAS.cLa

(Kopp et al., 1999)

Phenotype Manifest In

adult CSD interneuron, with Ctet\tetX^TNT-LC.UAS

(Roy et al., 2007)

adult CSD interneuron, with DCTN1-p150^Δ.UAS

(Roy et al., 2007)

adult CSD interneuron, with EcR^{B1-ΔC655.W650A.UAS}

(Roy et al., 2007)

adult CSD interneuron, with Hsap\KCNJ2^UAS.EGFP

(Roy et al., 2007)

adult CSD interneuron, with shi^1.UAS

(Roy et al., 2007)

cell body rind of medulla | somatic clone, with Eph^DN.UAS

(Dearborn et al., 2002)

dendritic tree, with Ctet\tetX^TNT-LC.UAS

(Roy et al., 2007)

denticle & embryonic epidermis | somatic clone, with shg^UAS.cSa

(Sanson et al., 1999)

denticle | ectopic | somatic clone, with shg^UAS.cSa

(Sanson et al., 1999)

denticle | somatic clone, with shg^UAS.cSa

(Sanson et al., 1999)

embryonic epidermis | somatic clone, with shg^UAS.cSa

(Sanson et al., 1999)

fat body | somatic clone, with Akt1^{UAS.Tag:HA,Tag:MyrPalm(Lck)}

(Tiefenböck et al., 2010)

fat body | somatic clone, with InR^UAS.cHa

(Tiefenböck et al., 2010, Baltzer et al., 2009)

fat body | somatic clone, with Myc^UAS.cSAa

(Baltzer et al., 2009)

fat body | somatic clone, with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Tiefenböck et al., 2010)

lamina, with ci^76.UAS

(Huang and Kunes, 1998)

lamina & neuron | precursor, with ci^76.UAS

(Huang and Kunes, 1998)

larval optic lobe & glial cell | somatic clone | cell non-autonomous, with Ras85D^N17.UAS

(Dearborn and Kunes, 2004)

lipid droplet | somatic clone, with InR^UAS.cHa

(Tiefenböck et al., 2010)

macrochaeta | somatic clone, with stg^UAS.cHa

(Negre et al., 2003)

medulla anlage | third instar larval stage, with Reph^UAS.cDa

(Dearborn et al., 2012)

medulla cortex & glial cell | somatic clone | cell non-autonomous, with Ras85D^N17.UAS

(Dearborn and Kunes, 2004)

microchaeta | somatic clone, with stg^UAS.cHa

(Negre et al., 2003)

optic cartridge, with btl::Egfr^λ.UAS

(Huang et al., 1998)

optic cartridge, with Egfr^DN.UAS

(Huang et al., 1998)

photoreceptor cell R7 & axon | somatic clone, with Eph^DN.UAS

(Dearborn et al., 2002)

photoreceptor cell R8 & axon | somatic clone, with Eph^DN.UAS

(Dearborn et al., 2002)

pleurum | somatic clone, with wg^UAS.cLa

(Kopp et al., 1999)

sternite, with dpp^UAS.cSa

(Kopp et al., 1999)

tergite, with dpp^UAS.cSa

(Kopp et al., 1999)

trichome | precursor | somatic clone, with ovo^B2.UAS

(Delon et al., 2003)

trichome | precursor | somatic clone, with ovo^D1.UAS.cAa

(Delon et al., 2003)

wing margin bristle | cell non-autonomous | ectopic | somatic clone, with dlp^UAS.Tag:HA

(Franch-Marro et al., 2005)

wing margin bristle | cell non-autonomous | somatic clone, with fz^UAS.cSa

(Katanaev et al., 2005)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^αTub84B.PP, yki^UAS.cHa has increased cell size | somatic clone phenotype, enhanceable by sd^UAS.cSa/Scer\GAL4^αTub84B.PP

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP, yki^UAS.cHa has hyperplasia | somatic clone phenotype, enhanceable by sd^UAS.cSa/Scer\GAL4^αTub84B.PP

(Ziosi et al., 2010)

NOT Enhanced by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, non-enhanceable by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Suppressed by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

Scer\GAL4^αTub84B.PP, yki^UAS.cHa has hyperplasia | somatic clone phenotype, suppressible | partially by sd^GD4744/Scer\GAL4^αTub84B.PP

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP, yki^UAS.cHa has increased cell size | somatic clone phenotype, suppressible by Scer\GAL4^αTub84B.PP/Myc^αTub84B.PBb

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP, yki^UAS.cHa has increased cell size | somatic clone phenotype, suppressible by Scer\GAL4^αTub84B.PP/Myc^PL35/Myc^αTub84B.PBb

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP, yki^UAS.cHa has hyperplasia | somatic clone phenotype, suppressible by Scer\GAL4^αTub84B.PP/Myc^GD1419

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP, yki^UAS.cHa has hyperplasia | somatic clone phenotype, suppressible by Scer\GAL4^αTub84B.PP/Myc^αTub84B.PBb

(Ziosi et al., 2010)

Myc^UAS.cSAa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, suppressible | partially by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, suppressible by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, suppressible by Ets97D^tne-1

(Baltzer et al., 2009)

Scer\GAL4^αTub84B.PP, dx^UAS.cMa has increased cell number | somatic clone phenotype, suppressible by Su(H)^del47

(Koelzer and Klein, 2006)

NOT suppressed by

Statement

Reference

Akt1^{UAS.Tag:HA,Tag:MyrPalm(Lck)}, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, non-suppressible by srl^KG08646

(Tiefenböck et al., 2010)

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, non-suppressible by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Enhancer of

Statement

Reference

sd^UAS.cSa/Scer\GAL4^αTub84B.PP is an enhancer of increased cell size | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

sd^UAS.cSa/Scer\GAL4^αTub84B.PP is an enhancer of hyperplasia | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PP/Myc^αTub84B.PBb is a suppressor of hyperplasia | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP/Myc^αTub84B.PBb is a suppressor of increased cell size | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP, Myc^PL35, Myc^αTub84B.PBb is a suppressor of increased cell size | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

sd^GD4744/Scer\GAL4^αTub84B.PP is a suppressor | partially of hyperplasia | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

Scer\GAL4^αTub84B.PP/Myc^GD1419 is a suppressor of hyperplasia | somatic clone phenotype of Scer\GAL4^αTub84B.PP, yki^UAS.cHa

(Ziosi et al., 2010)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, non-enhanceable by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Suppressed by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has lipid droplet | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

Myc^UAS.cSAa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, suppressible | partially by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, suppressible by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, suppressible by Ets97D^tne-1

(Baltzer et al., 2009)

Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^αTub84B.PP has mitochondrion | somatic clone phenotype, suppressible by Ets97D^tne-1

(Baltzer et al., 2009)

NOT suppressed by

Statement

Reference

Akt1^{UAS.Tag:HA,Tag:MyrPalm(Lck)}, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, non-suppressible by srl^KG08646

(Tiefenböck et al., 2010)

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, non-suppressible by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^αTub84B.PP, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi¹/spi^SCP2

(Huang et al., 1998)

Scer\GAL4^αTub84B.PP, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi^SCP2/spi²

(Huang et al., 1998)

Scer\GAL4^αTub84B.PP, spi^UAS.cSa, Scer\GAL4^hs.PB rescues spi^SCP2/spi³

(Huang et al., 1998)

Partially rescues

Scer\GAL4^αTub84B.PP/ds^UAS.cTa partially rescues ds⁰⁵¹⁴²

(Matakatsu and Blair, 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^αTub84B.PP

Scer\GAL4^αTub84B.PP

Name Synonyms

α-Tubulin at 84B promoter construct of Pignoni

(Pignoni, 1998.11.9)

Secondary FlyBase IDs

References (35)

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