Allele Dmel\vnd^Scer\UAS.cCa

General Information
Symbol	Dmel\vnd^Scer\UAS.cCa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Chu	FlyBase ID	FBal0094300
Feature type	allele	Associated gene	Dmel\vnd

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Chu et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An XhoI-XbaI vnd cDNA fragment containing the entire open reading frame is expressed under the control of Scer\UAS regulatory sequences. (Chu et al., 1998)
Carried in construct	P{UAS-vnd.C} (Schulz and Gajewski, 1999, Chu et al., 1998, Urbach et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| embryonic stage, with Scer\GAL4^sca-537.4 (Urbach et al., 2006)
Phenotype Manifest In
	aCC neuron, with Scer\GAL4^Kr.PM (Chu et al., 1998) aCC neuron, with Scer\GAL4^sca-537.4 (Chu et al., 1998) embryonic glial cell & embryonic deutocerebrum & stage 12 embryo, with Scer\GAL4^sca-537.4 (Urbach et al., 2006) embryonic glial cell & embryonic protocerebrum & stage 12 embryo, with Scer\GAL4^sca-537.4 (Urbach et al., 2006) embryonic glial cell & embryonic tritocerebrum & stage 12 embryo, with Scer\GAL4^sca-537.4 (Urbach et al., 2006) muscle founder cell \| ectopic, with Scer\GAL4^69B (Schulz and Gajewski, 1999) neuroblast of ventral nerve cord primordium \| ectopic, with Scer\GAL4^69B (Schulz and Gajewski, 1999) pCC neuron, with Scer\GAL4^Kr.PM (Chu et al., 1998) pCC neuron, with Scer\GAL4^sca-537.4 (Chu et al., 1998) RP2 motor neuron, with Scer\GAL4^Kr.PM (Chu et al., 1998) RP2 motor neuron, with Scer\GAL4^sca-537.4 (Chu et al., 1998) U neuron, with Scer\GAL4^Kr.PM (Chu et al., 1998)
Detailed Description
	Statement Reference Stage 12 embryos expressing vnd^Scer\UAS.cCa under the control of Scer\GAL4^sca-537.4 show a reduction in the number of glial cells in the protocerebrum, deutocerebrum and tritocerebrum. (Urbach et al., 2006) Expression of vnd^Scer\UAS.cCa under the control of Scer\GAL4^69B results in the formation of extra ventral neuroblasts and extra ventral muscle founder cells in the embryo. (Schulz and Gajewski, 1999) Embryos expressing vnd^Scer\UAS.cCa under the control of Scer\GAL4^sca-537.4 show duplications and occasionally triplications of GMC 1-1a-like and GMC 7-1a-like cells. Duplications and triplications of aCC/pCC neurons are seen at later stages. Deletions of RP2 are seen in 16% of hemisegments, while duplications of RP2 are seen in approximately 47% of hemisegments. Embryos expressing vnd^Scer\UAS.cCa under the control of Scer\GAL4^Kr.PM show duplications of U motor neurons. The RP2 neuron is often absent or its position is displaced. aCC/pCC neurons appear to be duplicated. (Chu et al., 1998)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Scer\GAL4^Kr.PM/vnd^Scer\UAS.cCa partially rescues vnd^D38 (Chu et al., 1998) Scer\GAL4^rho.PL/vnd^Scer\UAS.cCa partially rescues vnd^D38 (Chu et al., 1998)
Fails to rescue	vnd^Scer\UAS.cCa/Scer\GAL4^sca-537.4 fails to rescue vnd^D38 (Chu et al., 1998)
Comments	vnd^Scer\UAS.cCa fully rescues ventral S1 and S2 neuroblast formation in vnd^D38 embryos when expressed under the control of Scer\GAL4^Kr.PM. vnd^Scer\UAS.cCa variably rescues neuroblast formation in vnd^D38 embryos when expressed under the control of Scer\GAL4^rho.PL. (Chu et al., 1998)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	vnd^Scer\UAS.cCa vnd^UAS.cCa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Chu (Chu et al., 1998)
Secondary FlyBase IDs

References ( 3 )
Research paper	Urbach et al., 2006, Development 133(21): 4315--4330 Segment-specific requirements for dorsoventral patterning genes during early brain development in Drosophila. [FBrf0194392] Schulz and Gajewski, 1999, Oncogene 18(48): 6818--6823 Ventral neuroblasts and the heartless FGF receptor are required for muscle founder cell specification in Drosophila. [FBrf0123191] Chu et al., 1998, Genes Dev. 12(22): 3613--3624 Formation and specification of ventral neuroblasts is controlled by vnd in Drosophila neurogenesis. [FBrf0105792]

Allele Dmel\vndScer\UAS.cCa

Allele Dmel\vnd^Scer\UAS.cCa