FlyBase Allele Report: Dmel\nej[Q7]

General Information

Symbol

Dmel\nej^Q7

Species

D. melanogaster

Name

FlyBase ID

FBal0094386

Feature type

allele

Associated gene

Dmel\nej

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Smith et al., 2004, Marek et al., 2000, Florence and McGinnis, 1998)

Mutagen

ethyl methanesulfonate

(Florence and McGinnis, 1998)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Ghezzi et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Schimke immuno-osseous dysplasia

modeled by Marcal1^UAS.cBa

(Morimoto et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Marek et al., 2000)

abnormal neurophysiology

(Marek et al., 2000)

lethal (with nej^P)

(Kumar et al., 2004)

lethal - all die during embryonic stage | recessive

(Florence and McGinnis, 1998)

some die during embryonic stage | recessive

(Florence and McGinnis, 1998)

Phenotype Manifest In

embryonic/larval proventriculus | embryonic stage

(Florence and McGinnis, 1998)

embryonic antennal sense organ

(Florence and McGinnis, 1998)

median tooth

(Florence and McGinnis, 1998)

mouth hook

(Florence and McGinnis, 1998)

neuromuscular junction

(Marek et al., 2000)

neuromuscular junction & bouton

(Marek et al., 2000)

terminal organ | embryonic stage

(Florence and McGinnis, 1998)

Detailed Description

Statement

Reference

The homeotic phenotypes attributed to the original chromosome carrying nej^Q7 most likely arise from second site mutations because none of the deletion mutations have any homeotic effects.

(Smolik, 2009)

Heterozygous larvae show a decrease in presynaptic transmitter release (quantal content) without any change in quantal size at the neuromuscular junction. There is a significant (although moderate) increase in the number of boutons at the neuromuscular junction of muscles 4, 6 and 7 compared to wild type in nej^EP1149/nej^Q7, nej^EP1179/nej^Q7 or nej^Q7/+ larvae. No alteration in axon guidance in the embryonic central nervous system is seen.

(Marek et al., 2000)

Transheterozygotes of nej^Q7 with nej^S342 die at the embryonic/larval border. Transheterozygotes of nej^Q7 with nej^TA57 die in the embryonic stages. Embryonic cuticles of nej^TA57/nej^Q7 or nej^Q7/Y show maxillary and antennal sense organs often have a slight pattern deformation, mouth hooks and median tooth are reduced and the proventriculus is sclerotinized.

(Florence and McGinnis, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

nej[+]/nej^Q7 is an enhancer of partially lethal - majority die phenotype of Dfd¹³/Dfd³

(Florence and McGinnis, 1998)

Suppressor of

Statement

Reference

nej^Q7 is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

nej^Q7 is an enhancer of phenotype of qed^S140V

(Florence, 1999.7.22)

nej^Q7 is an enhancer of phenotype of qed¹

(Florence, 1999.7.22)

nej[+]/nej^Q7 is an enhancer of haltere & macrochaeta | ectopic phenotype of Ubx^M1

(Florence and McGinnis, 1998)

nej^Q7 is an enhancer of wing vein | ectopic phenotype of ci^D

(Florence and McGinnis, 1998)

NOT Enhancer of

Statement

Reference

nej^Q7, Scer\GAL4^30A is a non-enhancer of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Suppressor of

Statement

Reference

nej^Q7 is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

nej^Q7 is a suppressor of wing margin phenotype of ci^D

(Florence and McGinnis, 1998)

NOT Suppressor of

Statement

Reference

nej^Q7, Scer\GAL4^30A is a non-suppressor of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Suppresses the posterior wing margin phenotype of ci^D. Enhances the formation of ectopic wing veins in ci^D.

(Florence and McGinnis, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

nej^G0470

(Yin et al., 2007)

nej^G0350

(Yin et al., 2007)

nej^G0112

(Yin et al., 2007)

Comments

nej^+mSa does not rescue the lethality associated with nej^Q7, suggesting that the nej^Q7 chromosome carries a second site mutation in addition to nej^Q7.

nej^T:SV5\V5 does not rescue the lethality associated with nej^Q7, suggesting that the nej^Q7 chromosome carries a second site mutation in addition to nej^Q7.

(Smolik, 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5292

y¹ nej^Q7 v¹ f¹/Dp(1;Y)FF1, y⁺/C(1)DX, y¹ w¹ f¹

Kyoto

108153

y¹ nej^Q7 v¹ f¹/Dp(1;Y)FF1, y⁺/C(1)DX, y¹ w¹ f¹

Notes on Origin