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General Information
Symbol
Dmel\cryUAS.cEa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Emery
FlyBase ID
FBal0094553
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-cry
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a cry cDNA is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Scer\GAL4tim.Scer\UAS driven cryScer\UAS.cEa show significantly longer free-running periods than wild-type flies under blue light.

Responsiveness of flies expressing Scer\GAL4tim.Scer\UAS driven cryScer\UAS.cEa to magnetic fields is altered compared to wild-type flies. This response is blue light dependent. After experimental exposure to a 300 microT magnetic field, most of the Scer\GAL4tim.Scer\UAS driven cryScer\UAS.cEa expressing flies become arrhythmic and the remaining ones show large changes in their circadian free-running periods.

Flies expressing cryScer\UAS.cEa, under the control of Scer\GAL4P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 16.7% versus 13%).

When cryScer\UAS.cEa is driven in the lateral neurons by Scer\GAL4tim.PE, the mutant flies become hypersensitive to low-intensity illumination, especially at Zeitgeber time (ZT) 15, though a modest but reproducible effect is seen at ZT21.

When expression is driven by Scer\GAL4tim.PE, hypersensitive circadian responses to light are produced. The flies are much more sensitive to light at low intensities. The effect is particularly strong at ZT15, suggesting that cry levels are normally limiting at low light intensities. The magnitude of the effect is more variable at ZT21, in the advance zone.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
NOT Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Flies expressing perScer\UAS.cYa and cryScer\UAS.cEa, under the control of Scer\GAL4P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 15.7% versus 13%).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

In contrast to wild-type animals, cryb mutants expressing Scer\GAL4P2.4.Pdf driven cryScer\UAS.cEa do display circadian locomotor activity rhythms under constant light conditions.

Similarly to wild-type flies, the majority of cryb mutants expressing Scer\GAL4Mai179 driven cryScer\UAS.cEa do not display circadian locomotor activity rhythms under constant light conditions regardless of the presence or absence of Scer\GAL80Pdf.PS in the genotype.

Similarly to wild-type animals, most of the cryb mutants expressing Scer\GAL4tim.PE driven cryScer\UAS.cEa do not display locomotor activity rhythms under constant light conditions regardless of the presence or absence of Scer\GAL80Pdf.PS in the genotype.

The behavioural rhythmicity seen in cryb flies under intense constant light (wild-type flies are arrhythmic under such conditions) is rescued by expression of cryScer\UAS.cEa under the control of Scer\GAL4tim.PE; the rescued flies are arrhythmic.

Expression of cryScer\UAS.cEa under the control of Scer\GAL4tim.PE rescues the circadian phase-resetting defect seen in cryb flies and restores the wild-type arrhythmicity behaviour that is defective in cryb flies. When cryScer\UAS.cEa is driven by Scer\GAL4P2.4.Pdf, the phase setting defect is rescued at Zeitgeber time (ZT)21, but only partially at ZT15, and the wild-type arrhythmicity behaviour is only partially restored. When cryScer\UAS.cEa is driven by Scer\GAL4ninaE.PD, rescue of neither phenotype is seen. pers; cryb double mutant flies manifest pseudo free-running behaviour under LD conditions, indicative of an entrainment deficit. On addition of cryScer\UAS.cEa driven by Scer\GAL4tim.PE, pers; cryb flies show an activity pattern that resembles very closely that of pers flies. On addition of cryScer\UAS.cEa driven by Scer\GAL4tim.PE, norpA39; cryb flies show an activity pattern that resembles very closely that of norpA39 flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
cryScer\UAS.cEa
cryUAS.cEa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Emery
Secondary FlyBase IDs
    References (13)