FlyBase Allele Report: Dmel\babo[32]

General Information

Symbol

Dmel\babo³²

Species

D. melanogaster

Name

FlyBase ID

FBal0094581

Feature type

allele

Associated gene

Dmel\babo

Associated Insertion(s)

P{?cp70ZT}babo³²

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Gesualdi and Haerry, 2007, Brummel et al., 1999)

Mutagen

Delta2-3 transposase

(Brummel et al., 1999)

Progenitor genotype

P{cp70ZT}1

(Brummel et al., 1999)

Associated Insertion(s)

P{?cp70ZT}babo³²

Cytology

Description

babo³² contains a deletion that removes the start codon and part of the extracellular domain.

(Gesualdi and Haerry, 2007)

Deletion removing part of the P{cp70ZT} and extending into the babo transcription unit up to base pair 850. The deletion removes the translation start site and the first 53 amino acids.

(Brummel et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Brummel et al., 1999)

abnormal neuroanatomy (with babo⁵²)

(Takaesu et al., 2006)

abnormal neuroanatomy | embryonic stage

(Parker et al., 2006)

abnormal neuroanatomy | embryonic stage (with babo⁵²)

(Parker et al., 2006)

abnormal neuroanatomy | larval stage

(Brummel et al., 1999)

abnormal neuroanatomy | larval stage (with babo⁵²)

(Yu et al., 2013, Yu et al., 2013)

abnormal neuroanatomy | prepupal stage (with babo²⁶)

(Zhu et al., 2008)

abnormal neuroanatomy | prepupal stage (with babo⁵²)

(Zhu et al., 2008)

abnormal neuroanatomy | pupal stage (with babo⁵²)

(Zhu et al., 2008)

abnormal size | adult stage | germline clone (with babo⁵²)

(Brummel et al., 1999)

abnormal size | larval stage

(Brummel et al., 1999)

decreased cell number | prepupal stage (with babo⁵²)

(Zhu et al., 2008)

decreased occurrence of cell division | larval stage

(Brummel et al., 1999)

decreased occurrence of cell division | third instar larval stage (with babo⁵²)

(Zhu et al., 2008)

lethal | recessive

(Brummel et al., 1999)

long lived

(Fife et al., 2024)

Phenotype Manifest In

adult head capsule (with Df(2R)Np3)

(Shyamala and Bhat, 2002)

adult medulla neuropil associated glial cell (with babo⁵²)

(Zhu et al., 2008)

adult optic lobe

(Brummel et al., 1999)

anal pad

(Brummel et al., 1999)

anterior pupal spiracle (with babo⁵²)

(Pentek et al., 2009)

axon & photoreceptor cell

(Brummel et al., 1999)

axon & photoreceptor cell R7 (with babo⁵²)

(Zhu et al., 2008)

axon & photoreceptor cell R8 (with babo⁵²)

(Zhu et al., 2008)

brain (with babo⁵²)

(Zhu et al., 2008)

cell body rind of adult medulla (with babo⁵²)

(Zhu et al., 2008)

embryonic/larval anterior spiracle

(Brummel et al., 1999)

embryonic/larval neuromuscular junction | larval stage (with babo⁵²)

(Yu et al., 2013, Yu et al., 2013)

embryonic/larval optic lobe (with babo⁵²)

(Zhu et al., 2008)

embryonic brain

(Brummel et al., 1999)

ganglion mother cell & larval optic lobe (with babo⁵²)

(Zhu et al., 2008)

imaginal disc

(Brummel et al., 1999)

lamina (with babo⁵²)

(Zhu et al., 2008, Takaesu et al., 2006)

lamina anlage (with babo⁵²)

(Zhu et al., 2008)

lamina anlage glial cell (with babo⁵²)

(Zhu et al., 2008)

larva

(Brummel et al., 1999)

larval abdominal segmental nerve

(Parker et al., 2006)

larval abdominal segmental nerve (with babo⁵²)

(Parker et al., 2006)

larval inner optic anlage (with babo⁵²)

(Zhu et al., 2008)

larval intersegmental nerve

(Parker et al., 2006)

larval intersegmental nerve (with babo⁵²)

(Parker et al., 2006)

larval outer optic anlage (with babo⁵²)

(Zhu et al., 2008)

larval transverse nerve (with babo⁵²)

(Yu et al., 2013)

leg | pharate adult stage (with babo⁵²)

(Pentek et al., 2009)

medulla (with babo⁵²)

(Zhu et al., 2008)

medulla anlage (with babo²⁶)

(Zhu et al., 2008)

medulla anlage (with babo⁵²)

(Zhu et al., 2008, Takaesu et al., 2006)

medulla forming neuroblast (with babo⁵²)

(Zhu et al., 2008)

optic cartridge (with babo⁵²)

(Zhu et al., 2008)

photoreceptor cell R7 & axon (with babo⁵²)

(Takaesu et al., 2006)

photoreceptor cell R8 & axon (with babo⁵²)

(Takaesu et al., 2006)

wing disc

(Brummel et al., 1999)

Detailed Description

Statement

Reference

babo³² third instar larval C4da neurons do not show any obvious defects in the dendritic arborization, as compared to controls.

(Hoyer et al., 2018)

The transverse nerve makes ectopic connections at the neuromuscular junction in babo³²/babo⁵² larvae.

(Yu et al., 2013)

Ectopic connections are formed at the neuromuscular junction in babo³²/babo⁵² larvae.

(Yu et al., 2013)

babo³²/babo⁵² animals pupariate in the food, show defects in anterior spiracle eversion (43%) and a significant incidence of bent legs at pharate stages (64%).

(Pentek et al., 2009)

babo³²/babo⁵² prepupae have a smaller brain lobe than normal and a reduced lamina plexus. Axon projections of the R7/R8 photoreceptor cells to the medulla are abnormal, with their growth cones forming bundles instead of the normal regular lattice network seen in controls. Photoreceptor axon targeting is still highly perturbed in 3 day old pupae and rotation of the medulla and lamina relative to one another does not take place. In the most strongly affected cases, babo³²/babo⁵² mutants show a great reduction in the number of lamina cap and cartridge neurons. babo³²/babo⁵² third instar larvae have a disorganised and smaller medulla neuropil compared to wild type. The number of glial cells at both the lamina and medulla is reduced in babo³²/babo⁵² white prepupae compared to wild type.

The medulla neuropil is smaller than normal in babo²⁶/babo³² white prepupae.

The optic lobe is much smaller than wild type in babo³²/babo⁵² white prepupae. There is an approximately 50% decrease in the number of medial optic lobe neuroblasts compared to controls, a decrease in the number of ganglion mother cells produced from these medulla neuroblasts and a subsequent decrease in the number of maturing neurons within the medulla cortex. There is also a decrease in the number of lamina precursor cells and thus a decrease in the number of lamina cartridges and laminar neurons.

The number of cells in M phase in the developing optic lobes is significantly reduced in babo³²/babo⁵² third instar larvae compared to controls. The size of the outer proliferation center and inner proliferation center zones is reduced in these larvae, although they still contain many cells in S phase. The ratio of M-phase cells to S-phase cells is thus reduced in the mutant optic lobes compared to wild type.

(Zhu et al., 2008)

In babo³² mutant embryos, the ISNb and SNa branches enter the correct muscle fields but fail to extend correctly. In 24% of hemisegments, ISNb axons stall prematurely and in 20% of hemisegments the SNa fails to defasciculate resulting in loss of either dorsal or lateral branches.

Embryos derived from babo⁵² germline clones crossed to babo³² fathers show a greater penetrance of ISNb and SNa axon guidance defects compared to zygotic mutants.

(Parker et al., 2006)

Photoreceptor R1-R6 axons project into the brain and form a relatively normal but very reduced lamina in babo³²/babo⁵² mutant larvae, but R7 and R8 axons never form a normal lattice network and their growth cones are collapsed. The medulla neuropil is significantly reduced in size and altered in morphology in babo³²/babo⁵² mutant larvae.

(Takaesu et al., 2006)

100% of babo³²/Df(2R)Np3 animals exhibit head capsule defects.

(Shyamala and Bhat, 2002)

Homozygous larvae show slowed development, sort body and enlarged anal pads. Very few homozygotes progress to the pharate stage. Larvae do not leave food and do not evert their anterior spiracles. Adult structures of a babo⁵²/babo³²mutant derived from germ line clones are reduced in size. Axons from the developing eye project into the brain but do not find their targets. Brain hemispheres are reduced in size and show failure of active cell division, with no increase in cell death. Wing disc is reduced in size.

(Brummel et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

babo³² has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by daw[+]/daw³

(Parker et al., 2006)

Suppressed by

Statement

Reference

babo³²/babo⁵² has abnormal neuroanatomy phenotype, suppressible by CycA^C8LR1/CycA[+]

(Zhu et al., 2008)

Enhancer of

Statement

Reference

babo[+]/babo³² is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of daw³

(Parker et al., 2006)

NOT Enhancer of

Statement

Reference

babo[+]/babo³² is a non-enhancer of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

babo[+]/babo³² is a non-enhancer of visible phenotype of I-2^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^vg.PM, put^UAS.cMa

(Bennett et al., 2003)

NOT Suppressor of

Statement

Reference

babo[+]/babo³² is a non-suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

babo[+]/babo³² is a non-suppressor of visible phenotype of I-2^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^vg.PM, put^UAS.cMa

(Bennett et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

babo³² has larval intersegmental nerve phenotype, enhanceable by daw[+]/daw³

(Parker et al., 2006)

Suppressed by

Statement

Reference

babo³²/babo⁵² has axon & eye photoreceptor cell phenotype, suppressible by CycA^C8LR1/CycA[+]

(Zhu et al., 2008)

babo³²/babo⁵² has lamina phenotype, suppressible by CycA^C8LR1/CycA[+]

(Zhu et al., 2008)

Enhancer of

Statement

Reference

babo[+]/babo³² is an enhancer of larval intersegmental nerve phenotype of daw³

(Parker et al., 2006)

NOT Enhancer of

Statement

Reference

babo[+]/babo³² is a non-enhancer of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

babo[+]/babo³² is a non-enhancer of wing phenotype of I-2^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^vg.PM, put^UAS.cMa

(Bennett et al., 2003)

NOT Suppressor of

Statement

Reference

babo[+]/babo³² is a non-suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

babo[+]/babo³² is a non-suppressor of wing phenotype of I-2^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^vg.PM, put^UAS.cMa

(Bennett et al., 2003)

Other

Statement

Reference

babo³², ptc^H84 has adult head capsule phenotype

(Shyamala and Bhat, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The photoreceptor axon targeting and lamina neuron defects seen in babo³²/babo⁵² mutants are substantially suppressed by CycA^C8LR1/+.

(Zhu et al., 2008)

In daw³/+; babo³²/+; double mutant embryos, 20% of hemisegments show ISNb pathfinding defects as opposed to only 2% in daw³/+ single mutants and 4% in babo³²/+ single mutants.

(Parker et al., 2006)

5% of babo³², ptc^H84 double heterozygotes exhibit head capsule defects.

(Shyamala and Bhat, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

babo^k07737

(Brummel et al., 1999)

Rescued by

babo³²/babo²⁶ is rescued by Scer\GAL4^insc-Mz1407/babo^b.UAS/babo^a.UAS

(Zhu et al., 2008)

babo³²/babo²⁶ is rescued by babo^b.UAS/Scer\GAL4^wor.PA/babo^a.UAS

(Zhu et al., 2008)

babo³² is rescued by babo^+t12

(Brummel et al., 1999)

Not rescued by

babo³²/babo²⁶ is not rescued by babo^b.UAS/babo^a.UAS/Scer\GAL4^ey.PH

(Zhu et al., 2008)

babo³²/babo²⁶ is not rescued by babo^b.UAS/Scer\GAL4^repo/babo^a.UAS

(Zhu et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

5399

108204

Notes on Origin

Discoverer

Separable from: a second mutation on the chromosome, which when homozygous leads to developmental delay and severely reduced cell proliferation.

(Zhu et al., 2008)

Comments

Probably a null allele.

(Brummel et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

babo³²

(Fife et al., 2024, Hoyer et al., 2018, Sarov et al., 2016, Hevia and de Celis, 2013, Yu et al., 2013, Yu et al., 2013, Ellis et al., 2010, Pentek et al., 2009, Schuldiner et al., 2008, Zhu et al., 2008, Gesualdi and Haerry, 2007, Herrero et al., 2007, Parker et al., 2006, Takaesu et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (19)

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