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General Information
Symbol
Scer\GAL4tim.PE
Species
S. cerevisiae
Name
timeless promoter construct of Emery
FlyBase ID
FBal0094697
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tim-GAL4, timGAL4, timeless-GAL4, tim-GAL4-27
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a GAL4 driver is driven by a tim BamHI-SalI promoter fragment including 6kb upstream of the tim translation start site.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
CNS glial cell | subset

Comment: At the border between adjacent neuropils

pacemaker neuron

Comment: Authors state tim-gal4 is known to express in all clock neurons.

glial cell of adult brain

Comment: strong expression

eye

Comment: medium expression

Additional Information
Statement
Reference

Drives expression in pacemaker neurons at all developmental stages.

Scer\GAL4tim.PE transcript is expressed in the embryonic brain at stage 16 in 100 cells. These cells co-express tim protein.

The larval Pdf negative s-LNv neuron is concluded to have the same projection pattern as the larval PDF-positive s-LNv neurons.

Expression of Scer\GAL4tim.PE is seen in cells near sensilla on the head capsule, thorax and abdomen of the adult. Expression of Scer\GAL4tim.PE in seen within the abdominal portion of the adult midgut. Expression in the mouth is restricted to the posterior region.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Blocking clock function (using cycΔ.UAS.Tag:MYC or timUAS.cUa) of Scer\GAL4tim.PE neurons significantly reduces the circadian rhythmicity of adult emergence after transfer to constant darkness, compared to controls; there is also a reduction when Scer\GAL80phm.1.2 is present to restrict expression to a smaller subset of Scer\GAL4tim.PE neurons, but this is not significant for timUAS.cUa.

Slowing down clock function (using dcoL.UAS.Tag:MYC) of Scer\GAL4tim.PE neurons significantly slows down the periodicity of adult emergence after transfer to constant darkness, compared to controls; this also occurs when Scer\GAL80phm.1.2 is present to restrict expression to a smaller subset of Scer\GAL4tim.PE neurons. Speeding up clock function (using dcoS.UAS.Tag:MYC) of Scer\GAL4tim.PE neurons significantly speeds up the periodicity of adult emergence after transfer to constant darkness, compared to controls; this also occurs, to a lesser extent, when Scer\GAL80phm.1.2 is present to restrict expression to a smaller subset of Scer\GAL4tim.PE neurons.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescues
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
GAL4tim.PE
Scer\GAL4tim.PE
Name Synonyms
timeless promoter construct of Emery
Secondary FlyBase IDs
    References (139)