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General Information
Symbol
Dmel\CycB2
Species
D. melanogaster
Name
FlyBase ID
FBal0094855
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P-element resulting in small deletion removing the region encoding α-helix 1, a conserved element of the cyclin box.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

CycB2 homozygous germaria show an abnormal accumulation of undifferentiated, pre-cystoblast cells, as compared to controls, although about of third of them completely lack germline stem cells. These mutant germaria show an increased number of spectrosome-containing single cells, as compared to controls.

Mutant ovaries contain few or no germ cells.

Over 95% of embryos derived from CycB2/CycB3 ; CycB2xNRE.hb females have defects in the abdominal segments, the posterior terminalia, or both. In extreme cases, the embryos die with an open posterior hole and not segments following the third thoracic one. The defects appear to arise during nuclear cleavage cycles 9-13; the somatic nuclei in the posterior of the embryos delay progression through cycles 9-13 and ultimately lose contact with the embryonic cortex, falling into the interior.

Mutant embryos appear to start cytokinesis earlier than wild-type embryos. These embryos also appear to stay in cytokinesis longer than normal.

Mutant females have rudimentary ovaries and lay few eggs. Homozygotes and hemizygotes reach adulthood.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
NOT Enhancer of
Statement
Reference

CycB2 is a non-enhancer of visible phenotype of upd1GMR.PB

Suppressor of
NOT Suppressor of
Statement
Reference

CycB2 is a non-suppressor of visible phenotype of upd1GMR.PB

Other
Phenotype Manifest In
Enhancer of
Statement
Reference

CycB2 is an enhancer of cardioblast phenotype of CycAC8LR1

CycB2 is an enhancer of mitotic cell cycle phenotype of CycAC8LR1

NOT Enhancer of
Statement
Reference

CycB2 is a non-enhancer of eye phenotype of upd1GMR.PB

CycB2 is a non-enhancer of phenotype of Mybunspecified

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

CycB[+]/CycB2 is a non-suppressor of embryonic somatic muscle phenotype of fzrie28

CycB[+]/CycB2 is a non-suppressor of embryonic myoblast | dorsal closure stage phenotype of fzrie28

CycB2 is a non-suppressor of eye phenotype of upd1GMR.PB

CycB2 is a non-suppressor of phenotype of Mybunspecified

Other
Additional Comments
Genetic Interactions
Statement
Reference

Cell death induced by bsk knockdown in cells exhibiting chromosomal instability (through expression of mad2GD16427) is reduced by lengthening G[[2]] through a CycB2 heterozygous background.

Reducing the level of CycB, through a CycB2/+ background, greatly enhances the Scer\GAL4nos.UTR.T:Hsim\VP16>DetS125E.Scer\UAS.P\T.T:Avic\GFP gain-of-function phenotype. Further reduction in the level of CycB, through a CycB2/CycBKG08886 background, greatly enhances the Scer\GAL4nos.UTR.T:Hsim\VP16>DetS125E.Scer\UAS.P\T.T:Avic\GFP gain-of-function phenotype.

The nuclear division defects seen in embryos derived from homozygous XpdSH2137 females expressing XpdScer\UAS.T:SV5\V5 under the control of Scer\GAL4αTub84B.PL are partially rescued if the females are carrying CycB2/+.

Reduction of maternal CycB by one half (using the CycB2 allele) in the Capr2/Df(3L)Cat, Fmr13/+ background partially rescues the premature mitosis 14 phenotype seen in embryos of this genotype. (Reduction of the zygotic contribution of CycB has no effect.)

snf148 ; CycB2 double mutant ovaries are not tumourous and contain few or no germ cells, similar to the CycB2 single mutant phenotype.

The reduced size of the posterior compartment of the wing that is seen in animals expressing crolScer\UAS.cMa under the control of Scer\GAL4en-e16E is suppressed if they are also heterozygous for CycB2.

The lethality seen in mei-4129D males in which excision of an insertion of P{hswa} is induced during development is partly suppressed by CycB2/+.

The lethality seen in mei-4129D males in which excision of an insertion of P{hswa} is induced during development is completely suppressed if the female parent carries CycAC8LR1 and if the male progeny carries both CycAC8LR1 and CycB2.

The rough eye phenotype seen in mei-4129D ; DNApol-α180Emus304/+ adults is partially rescued by CycB2/+.

Adult viability of CycD2/Y ; CycB2/+ males is 90% of expected value.

The addition of CycB2 has no effect on Mybunspecified viability.

CycAC8LR1, CycB2 double mutants arrest mesodermal division at mitosis 15 a more severe phenotype than CycAC8LR1 alone. Also a further reduction in the number of myocardial cells is seen.

CycB3L6540 CycB- (CycB2 or Df(2R)599-5) double mutants have defective late mitotic figures during mitosis 15 and multipolar spindles at stage 13.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (31)