Open Close
General Information
Symbol
Dmel\C3GUAS.Tag:MYC,Tag:M(c-SRC)
Species
D. melanogaster
Name
FlyBase ID
FBal0094887
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Full length C3G tagged with 6 copies of the Tag:MYC tag is expressed under the control of UASt regulatory sequences. The C3G sequences are also tagged at the 5' end with Tag:M(c-SRC), to produce a membrane-targetted protein.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 driven by Scer\GAL4Mef2.PR at 25C results in embryonic lethality and severe muscle defects are seen during the embryonic stages compared to controls.

C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 driven by Scer\GAL4Mef2.PR at 18C allows development of larvae. The larvae display severe morphological muscle defects, disruption of actin organisation in somatic muscles and detachment of the ventral longitudinal muscles compared to controls.

Flies expressing C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 under the control of Scer\GAL4GMR.PF have a rough eye phenotype with varying degrees of severity depending on the P{UAS-C3G.T:Myc.T:Myr3} insertion line used. Flies with an intermediate phenotype have a moderately rough eye surface with differentiated lenses and bristles. All ommatidia contain supernumerary photoreceptor cells, with up to 5 or 6 additional cells in some cases. Most of these extra photoreceptor cells have small rhabdomeres and are located in the distal portion of the retina, suggesting that they are R7 cells. Some pigment cells are present, but they fail to form a lattice surrounding the ommatidia. Flies with a strong phenotype have reduced eyes and the surface appears rough and folded. There are very few bristles on the surface of the eye and lens facets are not discernible. The ommatidial lattice is severely disrupted; fewer clusters of photoreceptor cells are seen than normal, the number of cells per cluster and the arrangement of the cells is irregular and the rhabdomeres often do not span the entire thickness of the retina and have an elongated morphology. Wing discs expressing C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 under the control of Scer\GAL4dpp.blk1 show an increase in BrdU incorporation in a band corresponding to the domain of Scer\GAL4dpp.blk1 expression. Adult wings of flies expressing C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 under the control of Scer\GAL4dpp.blk1 show a massive broadening of the third vein.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

The eye phenotype caused by C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 expressed under the control of Scer\GAL4GMR.PF is dominantly suppressed by Ras85De1B, ksrS-638, rlx-3-30 or RrvB1. Sose4G or drke0A has no effect on the eye phenotype caused by C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 expressed under the control of Scer\GAL4GMR.PF. The wing vein phenotype seen in flies expressing C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 under the control of Scer\GAL4dpp.blk1 phenotype is dominantly suppressed by Ras85De1B, ksrS-638, rlx-3-30 or Df(3L)R-G7, and is unaffected by Sose4G or drke0A.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr-SRC
C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3
C3GUAS.T:Myc,T:Myr3
C3GUAS.Tag:MYC,Tag:M(c-SRC)
Name Synonyms
Secondary FlyBase IDs
    References (3)