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General Information
Symbol
Dmel\cacTS2
Species
D. melanogaster
Name
FlyBase ID
FBal0095278
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C11949202T

Reported nucleotide change:

C4714T

Amino acid change:

P1385S | cac-PA; P1385S | cac-PB; P1385S | cac-PC; P1385S | cac-PD; P1388S | cac-PE; P1388S | cac-PF; P1386S | cac-PG; P1386S | cac-PH; P1387S | cac-PI; P1387S | cac-PJ; P1387S | cac-PL; P1387S | cac-PM; P1491S | cac-PN; P1388S | cac-PO; P1386S | cac-PP; P1385S | cac-PS; P1385S | cac-PT; P1385S | cac-PU

Reported amino acid change:

P1388S

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C?T. Amino acid replacement: P1388S.

Nucleotide substitution: C4714T. Amino acid replacement: P1385S.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  epilepsy
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

dorsal medial muscle & synapse (with cacL13)

dorsal medial muscle & synapse (with Df(1)KA10)

dorsal medial muscle & synapse | conditional ts

intracoxal lateral levator muscle & synapse | conditional ts

Detailed Description
Statement
Reference

At room temperature, cacTS2 mutants have normal feeding, grooming, mating, activity levels, giant fiber electrophysiology and are not bang sensitive.

At room temperature, cacTS2 flies are seizure-resistant, with a higher seizure threshold (in response to high frequency stimulation) than wild type; at higher restrictive temperatures (38C), flies become seizure-sensitive (display spontaneous seizure-like dorsal longitudinal muscle activity) and display behavioural paralysis.

cacTS2 flies are paralysed rapidly at 38[o]C but exhibit only mild motor defects at 36[o]C.

Synaptic transmission is normal at the permissive temperature of 20[o]C, but a marked reduction in the excitatory postsynaptic current amplitude is seen at 36[o]C.

cacTS2 mutants are paralyzed at 38[o]c but resistant to paralysis at 36[o]C.

At 22[o]C, EJP amplitudes of cacTS2 are significantly reduced compared with controls.

cacTS2 larvae grown at 25[o]C exhibit reduced nerve-terminal morphology. There is no change at 22[o]C.

At 37[o]C, EJP amplitudes in cacTS2 mutants are significantly reduced compared to controls.

Heterozygous, homozygous and cacS/cacTS2 P1 stage pupae have a significantly increased heart rate and an increased strength of heart beat rhythmicity compared to controls.

In mutants exocytosis is decreased at non-permissive temperatures in presynaptic boutons (apparently not caused by a decrease in Ca2+ at the nonpermissive temperature). The evoked synaptic potentials (ESPs) in cytochalasin D-treated mutant preparations is reduced either at 1Hz or 10 Hz during incubation at 36oC for 20 minutes compared with that at 22oC, this is characteristic of a inhibition of exocytosis, but not endocytosis. No effect is seen on endocytosis at either permissive or non-permissive temperatures.

At 37oC, cacTS2 flies have severe motor defects. At 36oC, the flies are not paralysed. At 38oC, cacTS2 flies (reared at 20oC) paralyse, with a time for 50% paralysis of 0.28 +/- 0.04 minutes. cacTS2 flies reared at 23oC show paralysis at 38oC, with a time for 50% paralysis of 0.33 +/- 0.02 minutes. cacTS2/cacE(TS2)1 females (reared at 20oC) paralyse at 36oC, with a time for 50% paralysis of 0.31 +/- 0.03 minutes. At 38oC, cacTS2/cacsu(TS2)1 flies (reared at 20oC) show a time for 50% paralysis of 0.43 +/- 0.06 minutes. cacTS2/cacsu(TS2)2 flies reared at 23oC show paralysis at 38oC, with a time for 50% paralysis of 0.39 +/- 0.02 minutes. cacTS2 have a wild-type synaptic current at the dorsal longitudinal flight muscle neuromuscular synapse at 20oC, and a marked reduction in excitatory postsynaptic current amplitude at 36oC.

Intact cacTS2 males have a slightly lower than normal mating success. Mating success of cacTS2 males which have had their wings removed (compared to control flies with their wings removed) is more markedly impaired. Mutant males show higher than normal numbers of intrapulse cycles (CPP) among their song sounds. This mutant phenotype increases with increasing temperature. The pulse amplitudes are also heat sensitive, showing a steady increase as the temperature is raised. Intrapulse frequency tends to be lower than normal in mutant males. The interpulse interval (IPI) is significantly different from that of wild-type males at 30oC. Analysis of the average numbers of intrapulse cycles (CPP) per song bout of reasonable length indicates that at 30oC (but not at 20oC) mutant males generate song pulses containing larger than normal numbers of cycles. Mutant flies do not show defects in phototaxis. Room temperature ERG recordings from mutant flies appear normal looking with regard to the shapes of light-on transient spikes, light-coincident photoreceptor potentials, light-off transients and repolarisation times. At high temperature, repolarisation times are prolonged. Mutant males exposed to 29oC and then tested at 25oC are approximately 1/4 to 1/3 as active as wild-type flies. This locomotion defect is somewhat improved at 22-23oC. Mutant flies take longer to recover from mechanical shock than normal.

Homozygotes show rapid paralysis at 38oC. At 36oC, flies show spinning behaviour and a lack of coordination.

At 20oC, cacTS2 homozygotes show wild-type synaptic currents at the dorsal longitudinal flight muscle (DLM) neuromuscular synapses. Exposure to higher temperatures produces a marked reduction in the amplitude of the synaptic current compared to wild type. This phenotype is reversible, showing full recovery to wild type upon return to 20oC. The extent of the synaptic current reduction is dependent on temperature. A conditional reduction in the evoked synaptic current is also seen at the intracoxal lateral levator muscle synapse. Despite a marked reduction in the evoked synaptic current at 36oC, cacTS2 synapses show wild-type mEPSP amplitudes under the same conditions. Heterozygotes show wild-type synaptic currents at the DLM neuromuscular synapse at the restrictive temperature.

cacTS2 mutants show a conditional reduction in the excitatory postsynaptic current at neuromuscular synapses.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

cacTS2 has paralytic | heat sensitive phenotype, enhanceable by P32EC1/P32EC1

cacTS2 has neurophysiology defective | heat sensitive phenotype, enhanceable by P32EC1

cacTS2 has paralytic | heat sensitive phenotype, enhanceable by DabEC1

cacTS2 has paralytic | heat sensitive phenotype, enhanceable by E(TS2)22

cacTS2 has paralytic | recessive | heat sensitive phenotype, enhanceable by comt1

Suppressed by
Statement
Reference

cacTS2 has neurophysiology defective | adult stage | temperature conditional phenotype, suppressible | partially by parabss1/parabss1

cacTS2 has paralytic | adult stage | temperature conditional phenotype, suppressible | partially by parabss1/parabss1

NOT suppressed by
Statement
Reference

cacTS2 has neurophysiology defective | temperature conditional phenotype, non-suppressible by Scer\GAL4Toll-6-D42/Frq2UAS.cRPa

Enhancer of
Statement
Reference

cacTS2 is an enhancer of paralytic | recessive | heat sensitive phenotype of comt1

NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

cacTS2 is an enhancer of phenotype of comtunspecified

Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

cacTS2 suppresses bang sensitivity (reduces behavioral paralysis) in sdaiso7.8/sdaiso7.8, eas2/eas2 or parabss1/parabss1 flies at room temperature; this suppression is increased after brief heat shock. cacTS2/cacTS2 almost completely suppresses bang sensitivity (reduces behavioral paralysis) in parabss1/+ flies at room temperature. cacTS2/Y also speeds recovery time from paralysis and reduces refractory time in parabss1/parabss1 flies.

After brief heat shock, cacTS2/Y: suppresses the low seizure threshold in sdaiso7.8/sdaiso7.8 flies, almost restoring it to wild type levels; partially suppresses the low seizure threshold in eas2/eas2 flies; transiently suppresses the low seizure threshold in parabss1/parabss1 flies, even increasing the threshold to above wild type levels (seizure resistance) - the seizure threshold returns to parabss1/parabss1 levels after some minutes at room temperature.

parabss1/parabss1 suppresses the spontaneous seizure-like activity in the dorsal longitudinal muscle as well as behavioural paralysis seen at higher restrictive temperatures (38C) in cacTS2/cacTS2 flies.

Unlike cacTS2 alone, cacTS2 P32EC1 double mutants exhibit rapid paralysis at 36[o]C.

P32EC1 strongly enhances the cacTS2 synaptic transmission phenotype.

There are no obvious changes in basic synapse morphology in cacTS2 P32EC1 double mutants.

DabEC1 is an enhancer of cacTS2. cacTS2;;DabEC1 double mutants exhibit rapid paralysis at 36[o]C.

Overexpression of Frq2Scer\UAS.cRPa (under the control of Scer\GAL4D42) does not affect the EJP amplitude found in cacTS2 mutants at 22[o]C.

The presence of a cacTS2 mutant background suppresses the enhanced neurotransmitter release found upon overexpression of Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42 at both permissive and non-permissive temperatures.

Expression of Frq2Scer\UAS.cRPa under the control of Scer\GAL4D42 in a cacTS2 background at 22[o]C or 37[o]C does no change the number of synaptic boutons.

E(TS2)22 enhances the cacTS2 phenotype, resulting in paralysis at 37oC in E(TS2)22 cacTS2 mutants.

cacTS2 comt4 double mutant males generate song pulses whose intrapulse cycle (CPP) values are within the normal range.

At 36oC, cacTS2 comt1 double mutants show faster paralysis than comt1 single mutants at this temperature. At 38oC, cacTS2 comt1 double mutants show faster rapid paralysis than in cacTS2 single mutants at this temperature.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

cacL13 fails to complement the cacTS2 electrophysiological phenotype. cacTS2 complements the cacS phenotype at 20oC but fails to complement it at 36oC.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Arose in: comt1

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (21)