Allele Dmel\brk^Scer\UAS.cJa

General Information
Symbol	Dmel\brk^Scer\UAS.cJa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Jazwinska	FlyBase ID	FBal0095494
Feature type	allele	Associated gene	Dmel\brk

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Jazwinska et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of brk cDNA is governed by Scer\UAS regulatory sequences. (Jazwinska et al., 1999)
Carried in construct	P{UAS-brk.J} (Moreno et al., 2002, Muller et al., 2003, Klinedinst and Bodmer, 2003, Hasson et al., 2001, Ebner et al., 2002, Rusten et al., 2002, Cavodeassi et al., 2002, Kwon et al., 2004, Merabet et al., 2002, Chen and Schupbach, 2006, Cook et al., 2004, Adachi-Yamada and O'Connor, 2002, Jazwinska et al., 1999, Jazwinska et al., 1999, Milchanowski et al., 2004, Martin et al., 2004, Kango-Singh et al., 2003, Shivdasani and Ingham, 2003, Rogulja and Irvine, 2005, Oh and Irvine, 2011, Barrio et al., 2007, Weiss et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	cell lethal, with Scer\GAL4^Ubx.PdC (Moreno et al., 2002) decreased cell number, with Scer\GAL4^hh-Gal4 (Martin et al., 2004) decreased cell number, with Scer\GAL4^nub-AC-62 (Martin et al., 2004) decreased cell number \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) increased cell death, with Scer\GAL4^hh-Gal4 (Martin et al., 2004) increased cell death, with Scer\GAL4^nub-AC-62 (Martin et al., 2004) mitotic cell cycle defective \| cell autonomous \| somatic clone \| third instar larval stage (Rogulja and Irvine, 2005) mitotic cell cycle defective \| cell non-autonomous \| somatic clone \| third instar larval stage (Rogulja and Irvine, 2005) neuroanatomy defective, with Scer\GAL4^Kr.PM (Rusten et al., 2002) size defective \| adult stage, with Scer\GAL4^GMR.PF (Oh and Irvine, 2011) visible \| adult stage, with Scer\GAL4^GMR.PF (Oh and Irvine, 2011)
Phenotype Manifest In
	cardioblast, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Klinedinst and Bodmer, 2003) crystal cell, with Scer\GAL4^twi.PG (Milchanowski et al., 2004) dorsal abdominal cluster, with Scer\GAL4^Kr.PM (Rusten et al., 2002) embryonic/first instar larval cuticle, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Jazwinska et al., 1999) eye, with Scer\GAL4^GMR.PF (Oh and Irvine, 2011) imaginal disc, with Scer\GAL4^nub-AC-62 (Martin et al., 2004) lateral abdominal cluster, with Scer\GAL4^Kr.PM (Rusten et al., 2002) presumptive embryonic/larval peripheral nervous system, with Scer\GAL4^Kr.PM (Rusten et al., 2002) wing, with Scer\GAL4^bi-omb-Gal4 (Jazwinska et al., 1999) wing, with Scer\GAL4^Bx-MS1096 (Cook et al., 2004) wing, with Scer\GAL4^nub-AC-62 (Martin et al., 2004) wing, with Scer\GAL4^salm-459.2 (Barrio et al., 2007) wing \| heat sensitive, with Scer\GAL4^C-765 (Martin et al., 2004) wing disc, with Scer\GAL4^Ubx.PdC (Moreno et al., 2002) wing disc \| cell autonomous \| third instar larval stage (Rogulja and Irvine, 2005) wing disc \| cell non-autonomous \| medial \| third instar larval stage (Rogulja and Irvine, 2005) wing disc \| posterior compartment, with Scer\GAL4^hh-Gal4 (Martin et al., 2004) wing disc \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) wing vein, with Scer\GAL4^Bx-MS1096 (Cook et al., 2004) wing vein, with Scer\GAL4^C-765 (Cook et al., 2004) wing vein, with Scer\GAL4^salm-459.2 (Barrio et al., 2007) wing vein, with Scer\GAL4^vg.int2.1 (Cook et al., 2004)
Detailed Description
	Statement Reference Wing disc clones expressing brk[Scer\UAS.cJa] under the control of Scer\GAL4[Scer\FRT.Act5C] grow poorly and are rarely recovered. Expression of brk[Scer\UAS.cJa] under the control of Scer\GAL4[GMR.PF] produces flies with small eyes compared to controls. (Oh and Irvine, 2011) Expression of brk[Scer\UAS.cJa] using Scer\GAL4[salm-459.2] results in moderate wing size reduction and vein loss. (Barrio et al., 2007) Somatic clones of brk^Scer\UAS.cJa; Scer\GAL4^{Act5C.Switch.PR} cells in mid-third instar wing discs show autonomous decreases in numbers of cells in S-phase after 14 hours treatment with 20μg/ml RU486. However, if the edges of these clones are located medially they cause a large increase in the of cells in S-phase adjacent to the medially located edge of the clone. No increase in cell death occurs prior to 26 hours after the initiation of RU486 treatment. A small increase in apoptosis is seen at 48 hours. (Rogulja and Irvine, 2005) brk^Scer\UAS.cJa; Scer\GAL4^Bx-MS1096 flies have small wings in which some or all of the veins are shifted, truncated or completely missing. Wing veins L2 and L5 are particularly stringly affected. brk^Scer\UAS.cJa; Scer\GAL4^C-765 flies have a range of venation phenotypes, including selective loss or displacement of L2 and L5 veins, a single central vein (L3) or complete loss of veins. In brk^Scer\UAS.cJa; Scer\GAL4^vg.int2.1 flies the wing veins are crowded into the central region of the wing blade, close to the anterior-posterior compartment boundary. (Cook et al., 2004) brk^Scer\UAS.cJa; Scer\GAL4^C-765 and brk^Scer\UAS.cJa; Scer\GAL4^nub-AC-62 flies have reduced wings. The degree of size reduction in the wings of brk^Scer\UAS.cJa; Scer\GAL4^C-765 flies varies with temperature - it is much more severe in animals raised at 25^oC than 17^oC. This is also apparent in discs - with severe size reduction being see in wing leg and haltere discs of third instar larvae raised at 29^oC, and less in those raised at lower temperatures. There is a slight increase in the level of apoptosis in the wing pouch of late third instar brk^Scer\UAS.cJa; Scer\GAL4^nub-AC-62 wing discs, but there is also a decrease in the rate of cell division in the pouch, as measured by BrdU incorporation or cell division marker expression. A similar decrease in proliferation, restricted to the posterior compartment, is seen in brk^Scer\UAS.cJa; Scer\GAL4^hh-Gal4 wing discs. (Martin et al., 2004) Expression of brk^Scer\UAS.cJa under the control of Scer\GAL4^twi.PG blocks crystal cell development. Expression of brk^Scer\UAS.cJa under the control of Scer\GAL4^srp blocks crystal cell development. (Milchanowski et al., 2004) When brk^Scer\UAS.cJa is driven throughout the mesoderm by Scer\GAL4^how-24B and Scer\GAL4^twi.PG, there is a consierable reduction in cardiogenesis. (Klinedinst and Bodmer, 2003) Expression of brk^Scer\UAS.cJa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} has no effect on the testes. (Shivdasani and Ingham, 2003) Clones in the wing disc expressing brk^Scer\UAS.cJa under the control of Scer\GAL4^Ubx.PdC do not survive in the centre of the disc, surviving only in lateral regions. (Moreno et al., 2002) Expression of brk^Scer\UAS.cJa under the control of Scer\GAL4^Kr.PM results in a reduced number of neurons in the dorsal and lateral peripheral nervous system. Segments expressing brk^Scer\UAS.cJa show no increase in apoptosis compared to other abdominal segments that are not expressing brk^Scer\UAS.cJa. (Rusten et al., 2002) When expression is governed by Scer\GAL4^bi-omb-Gal4 the area of the wing blade is dramatically reduced, leaving the alula and costa largely unperturbed. Corresponding third larval instar discs are reduced and do not have the normal folded morphology. (Jazwinska et al., 1999) Embryos expressing brk^Scer\UAS.cJa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} have a completely ventralised cuticle. (Jazwinska et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} has increased cell number \| somatic clone phenotype, enhanceable by Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has size defective \| adult stage phenotype, suppressible by ban^GS/Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has size defective \| adult stage phenotype, suppressible by tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has size defective \| adult stage phenotype, suppressible by yki^{S168A.Scer\UAS.T:SV5\V5}/Scer\GAL4^GMR.PF (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has visible \| adult stage phenotype, suppressible by ban^GS/Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has visible \| adult stage phenotype, suppressible by tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has visible \| adult stage phenotype, suppressible by yki^{S168A.Scer\UAS.T:SV5\V5}/Scer\GAL4^GMR.PF (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has decreased cell number \| somatic clone phenotype, suppressible \| partially by Scer\GAL4^{Scer\FRT.Act5C}/yki^{S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has decreased cell number \| somatic clone phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has decreased cell number \| somatic clone phenotype, suppressible by ban^GS/Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) Scer\GAL4^Ubx.PdC, brk^Scer\UAS.cJa has cell lethal phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Ubx.PdC (Moreno et al., 2002) Scer\GAL4^Ubx.PdC, brk^Scer\UAS.cJa has cell lethal phenotype, suppressible by Scer\GAL4^Ubx.PdC/puc^Scer\UAS.cMa (Moreno et al., 2002)
NOT suppressed by
Statement Reference Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has decreased cell number \| somatic clone phenotype, non-suppressible by tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011)
Other
Statement Reference Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} has size defective \| adult stage phenotype (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} has visible \| adult stage phenotype (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} has increased cell number \| somatic clone phenotype (Oh and Irvine, 2011)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} has wing disc \| somatic clone phenotype, enhanceable by Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has eye phenotype, suppressible by ban^GS/Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has eye phenotype, suppressible by tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PF (Oh and Irvine, 2011) Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa has eye phenotype, suppressible by yki^{S168A.Scer\UAS.T:SV5\V5}/Scer\GAL4^GMR.PF (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has wing disc \| somatic clone phenotype, suppressible \| partially by Scer\GAL4^{Scer\FRT.Act5C}/yki^{S168A.Scer\UAS.T:SV5\V5} (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has wing disc \| somatic clone phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has wing disc \| somatic clone phenotype, suppressible by ban^GS/Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011) Scer\GAL4^Ubx.PdC, brk^Scer\UAS.cJa has wing disc phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Ubx.PdC (Moreno et al., 2002) Scer\GAL4^Ubx.PdC, brk^Scer\UAS.cJa has wing disc phenotype, suppressible by Scer\GAL4^Ubx.PdC/puc^Scer\UAS.cMa (Moreno et al., 2002)
NOT suppressed by
Statement Reference Scer\GAL4^nub-AC-62, brk^Scer\UAS.cJa has wing disc phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^nub-AC-62 (Martin et al., 2004) Scer\GAL4^nub-AC-62, brk^Scer\UAS.cJa has wing phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^nub-AC-62 (Martin et al., 2004) Scer\GAL4^nub-AC-62, brk^Scer\UAS.cJa has wing phenotype, non-suppressible by Scer\GAL4^nub-AC-62/puc^Scer\UAS.cMa (Martin et al., 2004) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa has wing disc \| somatic clone phenotype, non-suppressible by tkv^{Q253D.Scer\UAS.cNc}/Mad^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^{Scer\FRT.Act5C} (Oh and Irvine, 2011)
Suppressor of
Statement Reference brk^Scer\UAS.cJa/Scer\GAL4^bi-omb-Gal4 is a suppressor of eye \| ectopic phenotype of Scer\GAL4^bi-omb-Gal4, ey^Scer\UAS.cHa (Kango-Singh et al., 2003) brk^Scer\UAS.cJa/Scer\GAL4^dpp.blk1 is a suppressor of eye \| ectopic phenotype of Scer\GAL4^bi-omb-Gal4, ey^Scer\UAS.cHa (Kango-Singh et al., 2003) brk^Scer\UAS.cJa/Scer\GAL4^nub-AC-62 is a suppressor of wing disc phenotype of Scer\GAL4^nub-AC-62, tkv^{Q253D.Scer\UAS.cNb} (Martin et al., 2004) brk^Scer\UAS.cJa/Scer\GAL4^nub-AC-62 is a suppressor of wing phenotype of Scer\GAL4^nub-AC-62, tkv^{Q253D.Scer\UAS.cNb} (Martin et al., 2004)
Other
Statement Reference Scer\GAL4^GMR.PF, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} has eye phenotype (Oh and Irvine, 2011) Scer\GAL4^{Scer\FRT.Act5C}, brk^Scer\UAS.cJa, tkv^{Q253D.Scer\UAS.cNc}, yki^{S168A.Scer\UAS.T:SV5\V5} has wing disc \| somatic clone phenotype (Oh and Irvine, 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of yki[S168A.Scer\UAS.T:SV5\V5] partially suppresses the underproliferation seen when brk[Scer\UAS.cJa] is expressed in wing disc clones under the control of Scer\GAL4[Scer\FRT.Act5C]. Co-expression of tkv[Q253D.Scer\UAS.cNc] suppresses the growth defects further, producing some clone overgrowth, whilst the addition of Mad[Scer\UAS.T:Zzzz\FLAG] increases clone overgrowth even further. Co-expression of tkv[Q253D.Scer\UAS.cNc] and Mad[Scer\UAS.T:Zzzz\FLAG] does not suppress the growth and survival defects seen when brk[Scer\UAS.cJa] is expressed in wing disc clones under the control of Scer\GAL4[Scer\FRT.Act5C]. Co-expression of Mad[Scer\UAS.T:Zzzz\FLAG] and tkv[Q253D.Scer\UAS.cNc] suppresses the small eye phenotype seen when brk[Scer\UAS.cJa] is expressed under the control of Scer\GAL4[GMR.PF]. Expression of yki[S168A.Scer\UAS.T:SV5\V5] suppresses the small eye phenotype seen when brk[Scer\UAS.cJa] is expressed under the control of Scer\GAL4[GMR.PF]. When tkv[Q253D.Scer\UAS.cNc] is additionally expressed overgrown eyes are seen. Expression of ban[GS] suppresses the underproliferation seen when brk[Scer\UAS.cJa] is expressed in wing disc clones under the control of Scer\GAL4[Scer\FRT.Act5C]. The eye growth defects seen when brk[Scer\UAS.cJa] is expressed under the control of Scer\GAL4[GMR.PF] are also rescued. (Oh and Irvine, 2011) puc^Scer\UAS.cMa does not rescue with development in brk^Scer\UAS.cJa; Scer\GAL4^nub-AC-62 flies. BacA\p35^Scer\UAS.cHa does not suppress the reduction of wing pouch size seen in brk^Scer\UAS.cJa; Scer\GAL4^nub-AC-62 wing discs - even when 2 copies of BacA\p35^Scer\UAS.cHa are present. The reduction in wing size seen in brk^Scer\UAS.cJa; Scer\GAL4^nub-AC-62 flies is not suppressed by puc^Scer\UAS.cMa. (Martin et al., 2004) The addition of brk^Scer\UAS.cJa suppresses the ectopic eye phenotype seen in ey^Scer\UAS.cHa, Scer\GAL4^bi-omb-Gal4 animals. The addition of brk^Scer\UAS.cJa suppresses the ectopic eye phenotype seen in ey^Scer\UAS.cHa, Scer\GAL4^dpp.blk1 animals. (Kango-Singh et al., 2003) Co-expression of BacA\p35^Scer\UAS.cHa allows the survival of clones expressing brk^Scer\UAS.cJa under the control of Scer\GAL4^Ubx.PdC in the centre of the wing disc, although their proliferation is impaired. Co-expression of puc^Scer\UAS.cMa allows the survival of clones expressing brk^Scer\UAS.cJa under the control of Scer\GAL4^Ubx.PdC in the centre of the wing disc. (Moreno et al., 2002)
Xenogenetic Interactions
Statement Reference Co-expression of BacA\p35[Scer\UAS.cHa] allows recovery of brk[Scer\UAS.cJa] expressing clones under the control of Scer\GAL4[Scer\FRT.Act5C] in the larval wing disc. (Oh and Irvine, 2011)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	brk^Scer\UAS.cJa brk^UAS.cJa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Jazwinska (Jazwinska et al., 1999)
Secondary FlyBase IDs

References ( 22 )

Generate a list of	All references relating to this allele ( 22 )

List References by type	Research paper ( 22 )
Recent research papers ( 1 )
	Oh and Irvine, 2011, Dev. Cell 20(1): 109--122 Cooperative Regulation of Growth by Yorkie and Mad through bantam. [FBrf0212763] Show all research papers ...

Allele Dmel\brkScer\UAS.cJa

Allele Dmel\brk^Scer\UAS.cJa