The eye tumour phenotype of flies expressing Dl^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH and also carrying the P{GSV1}lola^GS88A8 insertion (which results in the lola^GS88A8 and psq^GS88A8 alleles being expressed under the control of Scer\GAL4^ey.PH) is dominantly enhanced by Rbf¹⁴.

The cell-autonomous block of cell cycle progression in the position of the second mitotic wave in Df(1)N-54l9 clones in the eye disc is not rescued when clones are also mutant for Rbf¹⁴.

Eye disc precluster cells mutant for both dap⁴ and Rbf¹⁴ continue to cycle instead of arresting. In addition, no G1 arrest occurs anterior to the morphogenetic furrow, all cells enter the second mitotic wave, and cells continue to cycle more posteriorly.

Rbf¹⁴ does not genetically interact with vri^unspecified in double heterozygous combination.

The early larval lethality of hemizygous males is partially suppressed by E2f⁹¹/E2f¹⁶⁴; these larvae survive to the third larval instar stage. Their development is delayed by about 2 days compared to wild type, and most of the larvae can develop further into pupae. The Rbf¹⁴/Y ; E2f⁹¹/E2f¹⁶⁴ larvae and pupae are slightly smaller than wild type. Some develop into very late stage pupae with adult structures (such as eyes, legs, wings, cuticle and bristles) but they cannot completely eclose. The pattern of BrdU incorporation in E2f⁹¹/E2f¹⁶⁴ eye discs is the same as wild type. There are two differences in the pattern of BrdU incorporation in the eye discs of Rbf¹⁴/Y ; E2f⁹¹/E2f¹⁶⁴ larvae compared to wild type. Firstly, the G₁ arrested region in the morphogenetic furrow is much narrower, cells just anterior to the morphogenetic furrow are not arrested in G₁, instead they become part of the first mitotic wave. Secondly, some cells posterior to the second mitotic wave still incorporate BrdU. Rbf¹⁴/Y ; E2f⁹¹/E2f¹⁶⁴ eye discs are slightly smaller than normal. There is increased cell death (seen just anterior to the morphogenetic furrow) in these eye discs compared to those of wild-type or E2f⁹¹/E2f¹⁶⁴ larvae. The phenotypes of adult Rbf¹⁴ flies rescued by expressing Rbf^hs.PD during the first 3 days after egg laying can be rescued without heat shock if the flies are also carrying E2f⁹¹/E2f¹⁶⁴. Approximately 50% of the expected number of Rbf¹⁴/Y ; E2fⁱ²/E2f^rM729 flies survive to the adult stage, and the development of these flies is not significantly delayed compared to sibling flies. The surviving flies have normal eyes, normal macrochaetae on the notum and normal wings and legs. The larval lethality of E2f⁹¹/E2f^rM729 transheterozygotes can be suppressed by Rbf^120a/Rbf¹⁴; the larvae develop into large late third instar larvae and many can pupate. Some Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 animals can develop into pharate adults with adult eyes, legs and wing structures. The development of these larvae is significantly retarded compared to wild-type larvae, with the earliest pupae observed at around day 11 after egg laying. BrdU incorporation is not seen in the optic lobe region of the brain in day 13 E2f⁹¹/E2f^rM729 larvae, in contrast to wild-type third instar larvae. The normal pattern of DNA replication in the optic lobe region is restored if the larvae are also carrying Rbf^120a/Rbf¹⁴. The eye discs of E2f⁹¹/E2f^rM729 larvae are very small and have only a few cells labelled with BrdU with no specific pattern. In Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 eye discs the overall pattern of DNA replication is normal. No significant cell death is seen in these eye discs. In late third instar discs there are about 25 rows of developing photoreceptor cells (as in wild-type discs) but there are much fewer cells anterior to the morphogenetic furrow than in wild type.

Enhances the rough eye phenotype of flies expressing both E2f^GMR.PD and Dp^GMR.PD.

Rbf¹⁴ does not suppress the degeneration seen in leg motor neuron clones expressing Hsap\TARDBP^{Q331K.Scer\UAS.cSa} under the control of Scer\GAL4^VGlut-OK371.

Dominantly suppresses the Hsap\CDKN1A^GMR.PH eye phenotype.