|Feature type||allele||Associated gene||Dmel\Gαs|
|Also Known As||dgsB19, B19|
|Allele class||hypomorphic allele - genetic evidence|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Nucleotide substitution: A1117T. Amino acid replacement: I373F.
Single amino acid substitution.
|Phenotype Manifest In|
In homozygous mutant larvae, the overall pattern of innervation is normal. In second instar homozygous larvae, the extent of synaptic branching is slightly reduced, but the number of synaptic boutons is not significantly different to controls. However, the number of synaptic boutons is significantly decreased in the wandering stage third instar larvae. Bouton numbers are further decreased in G-sα60AB19/Df(2R)or-BR11 larvae. Decreased numbers of boutons are associated with significant decrease in the extent of synaptic arborization in both homozygous and hemizygous larvae. Muscle and neuronal development is normal in these mutants. When nerves in mutant larvae are stimulated at 0.3 Hz, the average amplitudes of synaptic currents are normal. When the stimulus frequency is increased to 109 Hz for 50s, the amplitude of synaptic currents does not increase either during or immediately after tetanic stimulation.
G-sα60AB19 mutant third instar larvae show reduced facilitation during tetanus and lack of post-tetanic potentiation.
6% of homozygous embryos die. Homozygous, transheterozygous and hemizygous larvae survive for varying lengths of time, with a very few becoming pharate adults that never eclose. The mutant larvae are lethargic, grow more slowly and are thinner and more transparent due to reduced amounts of fat body compared to heterozygous siblings. Pupation of homozygous larvae is delayed by at least 1 day in uncrowded conditions, compared to control larvae. The pupae are deformed due to incomplete shortening of the body during pupariation and the larval mouthhooks are often not withdrawn into the pupal case. 30% of homozygous larvae pupate, compared to 11% of hemizygotes. Homozygous pharate adults have normal external morphology, but when removed from the pupal case are immobile. Third instar homozygous and hemizygous larvae crawl shorter distances than heterozygous controls. Some larvae crawl in continuous circles, backwards or on their backs for extended periods of time, behaviours that are not seen in wild type or heterozygotes. The larvae appear not to be attracted to yeast granules. Ovaries of females carrying homozygous germ-line clones appear normal. 97% of G-sα60AB19/G-sα60AR19 embryos derived from females carrying G-sα60AB19 homozygous germ-line clones do not have cuticle defects.
Animals die during the larval and pupal stages and never eclose. Development is delayed and larvae have behavioural abnormalities.
|Phenotype Manifest In|
|NOT Enhanced by|
|NOT suppressed by|
|NOT Enhancer of|
|NOT Suppressor of|
|Complementation & Rescue Data|
|Partially rescued by|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 7 )|
|Secondary FlyBase IDs|
|References ( 9 )|