FlyBase Allele Report: Dmel\Egfr[2.UAS]

General Information

Symbol

Dmel\Egfr^2.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0095739

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{Egfr.2.UAS}

Also Known As

UAS-Egfr type II, UAS-Egfr

Key Links

Transgenic product class

wild_type

(Lesokhin et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lesokhin et al., 1999)

Mutagen

in vitro construct

(Lesokhin et al., 1999)

Progenitor genotype

Carried in construct

P{Egfr.2.UAS}

Cytology

Description

Expression of wild type Type 2 Egfr coding region is governed by UASt regulatory sequences.

(Lesokhin et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Ras85D^{G12V.UAS.Tag:FLAG,Tag:polyHis}

(Washington et al., 2020)

modeled by Ras85D^{Y4F.G12V.UAS.Tag:FLAG,Tag:polyHis}

(Washington et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^phtm.PU

(Cavieres-Lepe et al., 2024)

Phenotype Manifest In

embryonic/larval tracheal section, with Scer\GAL4^btl.PS

(Ruiz et al., 2012)

eye, with Scer\GAL4^h-H10

(Lesokhin et al., 1999)

ommatidium, with Scer\GAL4^h-H10

(Lesokhin et al., 1999)

photoreceptor neuron | P-stage | ectopic, with Scer\GAL4^GMR.PF

(Yang and Baker, 2003)

tracheal lumen, with Scer\GAL4^btl.PS

(Ruiz et al., 2012)

wing, with Scer\GAL4^32B

(Lesokhin et al., 1999)

wing vein, with Scer\GAL4^32B

(Lesokhin et al., 1999)

Detailed Description

Statement

Reference

Overexpression of Egfr through expression of Egfr^2.Scer\UAS in the tracheal system under the control of Scer\GAL4^btl.PSresults in an increase in lumen formation, characterised by multiple air-filled lumens within a single cell.

(Ruiz et al., 2012)

The number of cells posterior to the furrow in eye discs that display caspase activation is reduced in Egfr^2.Scer\UAS; Scer\GAL4^GMR.PF animals. There is no accompanying increase in the levels of photoreceptor differentiation at this stage, but there is an increase in mitosis during the second mitotic wave (G2 to M progression, posterior to the furrow). The number of cells displaying caspase activation in pupal eye discs at 30 hours after puparium formation, is also significantly reduced in these animals, accompanied by significantly increased photoreceptor differentiation.

(Yang and Baker, 2003)

When expression is driven by Scer\GAL4^32B there is no effect on wing development. When expression is driven by Scer\GAL4^h-H10 there is no effect on eye development. When expression is driven at higher levels (induced by raised temperature) with Scer\GAL4^32B extra wing vein appears - similar to that caused by expression of Egfr^{2.A887T.Scer\UAS} at lower levels. When expression is driven at higher levels (induced by raised temperature) with Scer\GAL4^h-H10 mutant eye phenotype appears - similar to that caused by expression of Egfr^{2.A887T.Scer\UAS} at lower levels. Most cells are converted to photoreceptor fates.

(Lesokhin et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Egfr^2.UAS/Scer\GAL4^btl.PS is an enhancer of larval tracheal system phenotype of Ptp10D¹, Ptp4E¹

(Jeon and Zinn, 2009)

NOT Enhancer of

Statement

Reference

Egfr^2.UAS/Scer\GAL4^btl.PS is a non-enhancer of embryonic/larval tracheal section | somatic clone phenotype of Zpr1^31ZZ

(Ruiz et al., 2012)

Egfr^2.UAS/Scer\GAL4^btl.PS is a non-enhancer of tracheal lumen | somatic clone phenotype of Zpr1^31ZZ

(Ruiz et al., 2012)

NOT Suppressor of

Statement

Reference

Egfr^2.UAS/Scer\GAL4^btl.PS is a non-suppressor of embryonic/larval tracheal section | somatic clone phenotype of Zpr1^31ZZ

(Ruiz et al., 2012)

Egfr^2.UAS/Scer\GAL4^btl.PS is a non-suppressor of tracheal lumen | somatic clone phenotype of Zpr1^31ZZ

(Ruiz et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of Egfr^2.Scer\UAS or Egfr^{2.A887T.Scer\UAS} in Zpr1^31ZZ mutant MARCM tracheal cell clones does not alter the Zpr1^31ZZ gas-filling defect.

(Ruiz et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9535

w^*; P{Egfr.2.UAS}7-10

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Egfr^2.Scer\UAS

Egfr^2.UAS

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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