FlyBase Allele Report: Dmel\Egfr[2.A887T.UAS]

General Information

Symbol

Dmel\Egfr^2.A887T.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0095740

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{Egfr.2.A887T.UAS}

Also Known As

UAS-EGFR^CA

Key Links

Transgenic product class

missense_variant

(Lesokhin et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Lesokhin et al., 1999)

Mutagen

in vitro construct

(Lesokhin et al., 1999)

Progenitor genotype

Carried in construct

P{Egfr.2.A887T.UAS}

Cytology

Description

Expression of mutant Type 2 Egfr coding region (carries the amino acid replacement A887T) is governed by UASt regulatory sequences.

(Lesokhin et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA

(Nászai et al., 2021)

model of cancer

CEA with scrib⁶⁷³

(Sheng and Du, 2020)

CEA

(Brás et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intestinal cancer

is ameliorated by Rala^KK108989

(Nászai et al., 2021)

is exacerbated by Rala^UAS.cRa

(Nászai et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain | somatic clone, with Scer\GAL4^repo.PU

(Read et al., 2009)

adult optic lobe | adult stage | somatic clone, with Scer\GAL4^repo.PU

(Read et al., 2009)

adult posterior midgut epithelium, with Scer\GAL4^esg.PU

(Zhao et al., 2022)

adult posterior midgut epithelium | somatic clone, with Scer\GAL4^Act5C.PI

(Zhao et al., 2022)

eye, with Scer\GAL4^h-H10

(Lesokhin et al., 1999)

glial cell | adult stage | somatic clone | increased number, with Scer\GAL4^repo.PU

(Read et al., 2009)

ommatidium, with Scer\GAL4^h-H10

(Lesokhin et al., 1999)

wing, with Scer\GAL4^32B

(Lesokhin et al., 1999)

wing vein, with Scer\GAL4^32B

(Lesokhin et al., 1999)

Detailed Description

Statement

Reference

Adulthood-only expression of Egfr^2.A887T.UAS under the control of Scer\GAL4^esg.PU leads to an increase in mitotic index in the gut.

Adulthood-induced intestinal clones that express Egfr^2.A887T.UAS under the control of Scer\GAL4^Act5C.PI are larger than control clones.

(Zhao et al., 2022)

Expression of Egfr^{2.A887T.Scer\UAS} driven by Scer\GAL4^tin.CΔ4 results in a failure of flies to eclose from the late pupal stage.

(Yu et al., 2010)

Glial clones in adult brains expressing Egfr^{2.A887T.Scer\UAS} under the control of Scer\GAL4^repo.PU contain two-fold more cells than wild type.

Optic lobe clones expressing Egfr^{2.A887T.Scer\UAS} generated using eyFLP under the control of Scer\GAL4^repo.PU contain a modest number of excess and abnormal glia relative to wild type controls.

(Read et al., 2009)

When expression is driven by Scer\GAL4^32B extra vein tissue appears in the wing. Phenotype is more pronounced at elevated temperatures. When expression is driven by Scer\GAL4^h-H10 eyes show gaps in the array of ommatidia.

(Lesokhin et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU has abnormal neuroanatomy | adult stage | somatic clone phenotype, enhanceable by Pten¹¹⁷

(Read et al., 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU has neoplasia | adult stage | somatic clone phenotype, enhanceable by Pten¹¹⁷

(Read et al., 2009)

Suppressed by

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by dind^{RNAi.UAS.RNAi}, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by dind^NIG.7705R, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^Act5C.PI has increased cell number | somatic clone | adult stage phenotype, suppressible by Phb1²⁰/Phb1²⁰

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | larval stage phenotype, suppressible by psidin^55D4/psidin^55D4

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | larval stage phenotype, suppressible by psidin^{S678D.UAS.Tag:HA}, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has increased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by psidin^55D4/psidin^55D4

(Sheng and Du, 2020)

NOT suppressed by

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | larval stage phenotype, non-suppressible by psidin^{S678A.UAS.Tag:HA}, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | larval stage phenotype, non-suppressible by psidin^UAS.cKa, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Enhancer of

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU is an enhancer of neoplasia | adult stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^repo.PU

(Read et al., 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU is an enhancer of abnormal neuroanatomy | adult stage | somatic clone phenotype of Pten¹¹⁷, Scer\GAL4^repo.PU

(Read et al., 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU is an enhancer of neoplasia | adult stage | somatic clone phenotype of Pten¹¹⁷, Scer\GAL4^repo.PU

(Read et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/Egfr^2.A887T.UAS is a suppressor | partially of increased cell death | somatic clone | larval stage phenotype of Rbf^15aΔ, psidin^55D4

(Sheng and Du, 2020)

Scer\GAL4^Act.PU/Egfr^2.A887T.UAS is a suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

Other

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has neoplasia | somatic clone | larval stage phenotype

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has increased occurrence of cell division | somatic clone | larval stage phenotype

(Sheng and Du, 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU has glial cell | adult stage | increased number | somatic clone phenotype, enhanceable by Pten¹¹⁷

(Read et al., 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU has adult brain | somatic clone phenotype, enhanceable by Pten¹¹⁷

(Read et al., 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU has adult optic lobe | adult stage | somatic clone phenotype, enhanceable by Pten¹¹⁷

(Read et al., 2009)

Suppressed by

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has adult posterior midgut epithelium phenotype, suppressible by dind^{RNAi.UAS.RNAi}, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has adult posterior midgut epithelium phenotype, suppressible by dind^NIG.7705R, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^esg.PU has adult posterior midgut epithelium phenotype, suppressible by Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^Act5C.PI has adult posterior midgut epithelium | somatic clone phenotype, suppressible by Phb1²⁰/Phb1²⁰

(Zhao et al., 2022)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has larval brain | somatic clone | larval stage phenotype, suppressible by psidin^55D4/psidin^55D4

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has gonad | somatic clone | larval stage phenotype, suppressible by psidin^55D4/psidin^55D4

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has larval brain | somatic clone | larval stage phenotype, suppressible by psidin^{S678D.UAS.Tag:HA}, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has gonad | somatic clone | larval stage phenotype, suppressible by psidin^{S678D.UAS.Tag:HA}, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

NOT suppressed by

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has larval brain | somatic clone | larval stage phenotype, non-suppressible by psidin^{S678A.UAS.Tag:HA}, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has gonad | somatic clone | larval stage phenotype, non-suppressible by psidin^{S678A.UAS.Tag:HA}, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has larval brain | somatic clone | larval stage phenotype, non-suppressible by psidin^UAS.cKa, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has gonad | somatic clone | larval stage phenotype, non-suppressible by psidin^UAS.cKa, psidin^55D4/psidin^55D4/Scer\GAL4^Act.PU

(Sheng and Du, 2020)

Enhancer of

Statement

Reference

Scer\GAL4^btl.PS/Egfr^2.A887T.UAS is an enhancer of larval tracheal system phenotype of Ptp10D¹, Ptp4E¹

(Jeon and Zinn, 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU is an enhancer of glial cell | adult stage | increased number | somatic clone phenotype of Pten¹¹⁷, Scer\GAL4^repo.PU

(Read et al., 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU is an enhancer of adult brain | somatic clone phenotype of Pten¹¹⁷, Scer\GAL4^repo.PU

(Read et al., 2009)

Egfr^2.A887T.UAS, Scer\GAL4^repo.PU is an enhancer of adult optic lobe | adult stage | somatic clone phenotype of Pten¹¹⁷, Scer\GAL4^repo.PU

(Read et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/Egfr^2.A887T.UAS is a suppressor | partially of eye disc | somatic clone | larval stage phenotype of Rbf^15aΔ, psidin^55D4

(Sheng and Du, 2020)

Scer\GAL4^Act.PU/Egfr^2.A887T.UAS is a suppressor | somatic clone of eye disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

Other

Statement

Reference

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has larval brain | somatic clone | larval stage phenotype

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³ has gonad | somatic clone | larval stage phenotype

(Sheng and Du, 2020)

Egfr^2.A887T.UAS, Ptp10D¹, Ptp4E¹, Scer\GAL4^btl.PS has embryonic/larval dorsal tracheal branch phenotype

(Jeon and Zinn, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

EyFLP-induced clones that both express Egfr^2.A887T.UAS under the control of Scer\GAL4^Act.PU and are scrib⁶⁷³/scrib⁶⁷³ form cephalic and gonadal tumors. The gonadal tumors are highly proliferative compared to neighboring control tissue.

(Sheng and Du, 2020)

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ;Stam¹⁹ double homozygous somatic MARCM clones in third instar larval eye disc is strongly suppressed by expression of Egfr^{2.A887T.Scer\UAS} under the control of the Scer\GAL4^Act.PU driver in the mutant clones.

(Sheng et al., 2016)

Expression of Egfr^{2.A887T.Scer\UAS} under the control of Scer\GAL4^btl.PS enhances the tracheal defects seen in Ptp10D¹ Ptp4E¹ double mutant embryos; the increase in diameter of the transverse connective/lateral trunk junction is further enhanced, and cysts appear on all dorsal branches.

(Jeon and Zinn, 2009)

Egfr^{2.A887T.Scer\UAS} Pten¹¹⁷ double mutant clones generated under the control of Scer\GAL4^repo.PU are overgrown and invasive. Cells from mutant clones appear to invade the brain, typically following fibre tracts, and sometimes inducing the formation of trachea. Mutant cells often penetrate deep into the brain. Smaller clones are commonly seen that are composed of relatively differentiated, enlarged glia with diffusely invasive projections.

Egfr^{2.A887T.Scer\UAS} Pten¹¹⁷ double mutant clones generated using eyFLP under the control of Scer\GAL4^repo.PU contain more excess glial cells than in either mutant alone. The clones are composed of 10s of glial cells in third instar larvae, becoming large invasive tumors composed of hundreds to thousands of cells in adults.

(Read et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9533

w^*; P{Egfr.2.A887T.UAS}8-2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EGFR^A887T

(Nászai et al., 2021)

Egfr^{2.A887T.Scer\UAS}

Egfr^2.A887T.UAS

Egfr^CA

(Proske et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

Open Close