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General Information
Symbol
Dmel\Egfr2.A887T.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0095740
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-EGFRCA
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of mutant Type 2 Egfr coding region (carries the amino acid replacement A887T) is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Egfr2.A887T.Scer\UAS driven by Scer\GAL4tin.CΔ4 results in a failure of flies to eclose from the late pupal stage.

Glial clones in adult brains expressing Egfr2.A887T.Scer\UAS under the control of Scer\GAL4repo.PU contain two-fold more cells than wild type.

Optic lobe clones expressing Egfr2.A887T.Scer\UAS generated using eyFLP under the control of Scer\GAL4repo.PU contain a modest number of excess and abnormal glia relative to wild type controls.

When expression is driven by Scer\GAL432B extra vein tissue appears in the wing. Phenotype is more pronounced at elevated temperatures. When expression is driven by Scer\GAL4h-H10 eyes show gaps in the array of ommatidia.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ;Stam19 double homozygous somatic MARCM clones in third instar larval eye disc is strongly suppressed by expression of Egfr2.A887T.Scer\UAS under the control of the Scer\GAL4Act.PU driver in the mutant clones.

Expression of Egfr2.A887T.Scer\UAS under the control of Scer\GAL4btl.PS enhances the tracheal defects seen in Ptp10D1 Ptp4E1 double mutant embryos; the increase in diameter of the transverse connective/lateral trunk junction is further enhanced, and cysts appear on all dorsal branches.

Egfr2.A887T.Scer\UAS Pten117 double mutant clones generated under the control of Scer\GAL4repo.PU are overgrown and invasive. Cells from mutant clones appear to invade the brain, typically following fibre tracts, and sometimes inducing the formation of trachea. Mutant cells often penetrate deep into the brain. Smaller clones are commonly seen that are composed of relatively differentiated, enlarged glia with diffusely invasive projections.

Egfr2.A887T.Scer\UAS Pten117 double mutant clones generated using eyFLP under the control of Scer\GAL4repo.PU contain more excess glial cells than in either mutant alone. The clones are composed of 10s of glial cells in third instar larvae, becoming large invasive tumors composed of hundreds to thousands of cells in adults.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Egfr2.A887T.Scer\UAS
Egfr2.A887T.UAS
Name Synonyms
Secondary FlyBase IDs
    References (8)