The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ;Stam19 double homozygous somatic MARCM clones in third instar larval eye disc is strongly suppressed by expression of Egfr2.A887T.Scer\UAS under the control of the Scer\GAL4Act.PU driver in the mutant clones.
Expression of Egfr2.A887T.Scer\UAS under the control of Scer\GAL4btl.PS enhances the tracheal defects seen in Ptp10D1 Ptp4E1 double mutant embryos; the increase in diameter of the transverse connective/lateral trunk junction is further enhanced, and cysts appear on all dorsal branches.
Egfr2.A887T.Scer\UAS Pten117 double mutant clones generated under the control of Scer\GAL4repo.PU are overgrown and invasive. Cells from mutant clones appear to invade the brain, typically following fibre tracts, and sometimes inducing the formation of trachea. Mutant cells often penetrate deep into the brain. Smaller clones are commonly seen that are composed of relatively differentiated, enlarged glia with diffusely invasive projections.
Egfr2.A887T.Scer\UAS Pten117 double mutant clones generated using eyFLP under the control of Scer\GAL4repo.PU contain more excess glial cells than in either mutant alone. The clones are composed of 10s of glial cells in third instar larvae, becoming large invasive tumors composed of hundreds to thousands of cells in adults.