Allele Dmel\AblScer\UAS.cFa
| General Information | |||
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| Symbol | Dmel\AblScer\UAS.cFa | Species | D. melanogaster |
| Name | Saccharomyces cerevisiae UAS construct a of Fogerty | FlyBase ID | FBal0095895 |
| Feature type | allele | Associated gene | Dmel\Abl |
| Allele class | |||
| Mutagen | in vitro construct - regulatory fusion | ||
Recent Updates
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| Description |
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| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Nature of the Allele
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| Allele class | |||
| Mutagen | |||
| Mutations Mapped to the Genome | |||
Type Location Additional Notes References | |||
| Associated Sequence Data | |||
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EMBL / GenBank | DNA sequence Protein sequence Name | ||
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| Progenitor genotype | |||
| Nature of the lesion | Statement Reference | ||
| Carried in construct | |||
| Cytology | |||
Phenotypic Data
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Phenotypic Class
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Phenotype Manifest In
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eye, with Scer\GAL4GMR.PU ventral adult lateral neuron & axon, with Scer\GAL4P2.4.Pdf | |||
Detailed Description
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Statement Reference Expression of Abl[Scer\UAS.cFa] under the control of Scer\GAL4[GMR.PF] does not affect eye development at 25[o]C, but when the temperature is increased to 29.5[o]C, increasing the expression level of Abl[Scer\UAS.cFa], retinal development is disrupted. The retina in these mutants increases in size (as evidenced by the outward bulging of the retina).
Expression of Abl[Scer\UAS.cFa] under the control of Scer\GAL4[1407] results in ISNb axons occasionally failing to innervate their target muscles and instead following ISN, leading to a 'bypass' phenotype. Expression of Abl[Scer\UAS.cFa] under the control of Scer\GAL4[GMR.PU] results in a rough eye phenotype. Overexpression of AblScer\UAS.cFa, under the control of Scer\GAL4P2.4.Pdf, induces a small increase in axonal outgrowth of the sLNv. Embryos expressing AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu show axons ectopically crossing the midline. In addition, ISNb axons in these embryos show an " ISNb bypass" phenotype, often failing to enter their ventral target domain (muscles 7/6, 13 and 12). Once they have passed their targets, the misguided ISNb axons often make contacts with muscle 12 ("reach-back" phenotype). AblScer\UAS.cFa animals exhibit a ISNb bypass phenotype. Flies expressing AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF have a mild rough eye phenotype. Expression of AblScer\UAS.cFa under the control of Scer\GAL4elav-C155 results in a low level of guidance errors in the ISNb. Expression of AblScer\UAS.cFa under the control of Scer\GAL4hs.PB results in multilayering of the follicle epithelium. Expression of AblScer\UAS.cFa under the control of Scer\GAL4T155 has little effect on follicle cells. When AblScer\UAS.cFa is expressed under the control if Scer\GAL4elav.PLu or Scer\GAL4sca-537.4, the bundles of longitudinal connectives of the medial pathway ectopically crosses the midline, in a dose dependant manner. Embryos expressing AblScer\UAS.cFa under the control of Scer\GAL431 have no visible defects in the central nervous system. Flies expressing AblScer\UAS.cFa under the control of Scer\GAL4hs.2sev are viable and fertile and have a mild rough eye phenotype; ommatidia are often fused and mechanosensory bristles are occasionally misplaced. Ommatidia are irregular in shape and size and show an altered trapezoid orientation of photoreceptor cells. The arrangement of the ommatidia is disrupted. Most, but not all, ommatidia contain the correct number of photoreceptor cells. ISNb axons often show a bypass phenotype in embryos expressing AblScer\UAS.cFa under the control of Scer\GAL41407 or Scer\GAL4elav.PLu. | |||
External Data
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Interactions
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Phenotypic Class
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Enhanced by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4elav-C155 has neuroanatomy defective phenotype, enhanceable by roboScer\UAS.cKa AblScer\UAS.cFa, Scer\GAL4elav-C155 has neuroanatomy defective phenotype, enhanceable by roboΔCC3.Scer\UAS AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has visible phenotype, enhanceable by leaScer\UAS.cSa AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has visible phenotype, enhanceable by robo3Scer\UAS.cSa AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has visible phenotype, enhanceable by roboScer\UAS.cKa AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has visible phenotype, enhanceable by roboΔCC2.Scer\UAS AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has visible phenotype, enhanceable by roboΔCC3.Scer\UAS AblScer\UAS.cFa, Scer\GAL4GMR.PU has visible phenotype, enhanceable by Ptp61FdsRNA.Scer\UAS.WIZ, Scer\GAL4GMR.PU | |||
Suppressed by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL41407 has neuroanatomy defective phenotype, suppressible by mspsd03376, Scer\GAL41407 AblScer\UAS.cFa, Scer\GAL4elav.PLu has neuroanatomy defective phenotype, suppressible | partially by chb4/chb4 AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has size defective | heat sensitive phenotype, suppressible | partially by mspsP, Scer\GAL4GMR.PF AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has size defective | heat sensitive phenotype, suppressible by mspsd03376, Scer\GAL4GMR.PF AblScer\UAS.cFa, Scer\GAL4GMR.PU has visible phenotype, suppressible by Ptp61Fm.Scer\UAS, Scer\GAL4GMR.PU | |||
NOT suppressed by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4P2.4.Pdf has neuroanatomy defective phenotype, non-suppressible by bskDN.Scer\UAS | |||
Enhancer of | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4GMR.PU is an enhancer of visible phenotype of HemScer\UAS.T:Myr-Src64B, Scer\GAL4GMR.PU AblScer\UAS.cFa is an enhancer of neuroanatomy defective phenotype of Scer\GAL4elav-C155, roboScer\UAS.cKa AblScer\UAS.cFa is an enhancer of neuroanatomy defective phenotype of Scer\GAL4elav-C155, roboΔCC3.Scer\UAS | |||
Suppressor of | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4GMR.PF is a suppressor of size defective | heat sensitive phenotype of Scer\GAL4GMR.PF, mspsd03376 Scer\GAL431/AblScer\UAS.cFa is a suppressor | partially of lethal phenotype of Abl1/Df(3L)st-j7, NrtM2 Scer\GAL4elav.PU/AblScer\UAS.cFa is a suppressor | partially of neuroanatomy defective | maternal effect | embryonic stage phenotype of Dab2/Dab1 | |||
Phenotype Manifest In
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Enhanced by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4elav-C155 has intersegmental nerve phenotype, enhanceable by roboScer\UAS.cKa AblScer\UAS.cFa, Scer\GAL4elav-C155 has intersegmental nerve phenotype, enhanceable by roboΔCC3.Scer\UAS AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has ommatidium phenotype, enhanceable by leaScer\UAS.cSa AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has ommatidium phenotype, enhanceable by robo3Scer\UAS.cSa AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has ommatidium phenotype, enhanceable by roboScer\UAS.cKa AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has ommatidium phenotype, enhanceable by roboΔCC2.Scer\UAS AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has ommatidium phenotype, enhanceable by roboΔCC3.Scer\UAS AblScer\UAS.cFa, Scer\GAL4GMR.PU has eye phenotype, enhanceable by Ptp61FdsRNA.Scer\UAS.WIZ, Scer\GAL4GMR.PU | |||
Suppressed by | |||
Statement Reference AblScer\UAS.cFa, LarScer\UAS.cKa has intersegmental nerve phenotype, suppressible | partially by Scer\GAL4elav.PLu AblScer\UAS.cFa, Scer\GAL41407 has intersegmental nerve branch ISNb of A1-7 phenotype, suppressible by mspsd03376, Scer\GAL41407 AblScer\UAS.cFa, Scer\GAL4elav.PLu has intersegmental nerve phenotype, suppressible | partially by chb4/chb4 AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has eye | heat sensitive phenotype, suppressible | partially by mspsP, Scer\GAL4GMR.PF AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has eye | heat sensitive phenotype, suppressible by mspsd03376, Scer\GAL4GMR.PF AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has retina | heat sensitive phenotype, suppressible | partially by mspsP, Scer\GAL4GMR.PF AblScer\UAS.cFa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF has retina | heat sensitive phenotype, suppressible by mspsd03376, Scer\GAL4GMR.PF AblScer\UAS.cFa, Scer\GAL4GMR.PU has eye phenotype, suppressible by Ptp61Fm.Scer\UAS, Scer\GAL4GMR.PU AblScer\UAS.cFa, Scer\GAL4unspecified has intersegmental nerve phenotype, suppressible by NScer\UAS.cBa | |||
NOT suppressed by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by bskDN.Scer\UAS | |||
Enhancer of | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4GMR.PU is an enhancer of eye phenotype of HemScer\UAS.T:Myr-Src64B, Scer\GAL4GMR.PU AblScer\UAS.cFa is an enhancer of intersegmental nerve phenotype of Scer\GAL4elav-C155, roboScer\UAS.cKa AblScer\UAS.cFa is an enhancer of intersegmental nerve phenotype of Scer\GAL4elav-C155, roboΔCC3.Scer\UAS Scer\GAL4unspecified/AblScer\UAS.cFa is an enhancer of intersegmental nerve | heat sensitive phenotype of Nl1N-ts1 | |||
Suppressor of | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4GMR.PF is a suppressor of retina | heat sensitive phenotype of Scer\GAL4GMR.PF, mspsd03376 Scer\GAL4elav.PU/AblScer\UAS.cFa is a suppressor | partially of intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype of Dab2/Dab1 | |||
Additional Comments
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Genetic Interactions
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Statement Reference Co-expression of msps[d03376] under the control of Scer\GAL4[1407] suppressed the ISNb bypass phenotype seen upon expression of Abl[Scer\UAS.cFa].
Co-expression of msps[P] with Abl[Scer\UAS.cFa] under the control of Scer\GAL4[GMR.PF] partially suppresses the Abl[Scer\UAS.cFa] retinal size increase.
Co-expression of msps[d03376] and Abl[Scer\UAS.cFa] under the control of Scer\GAL4[GMR.PF] neutralizes the opposing effects on retinal size, resulting in an eye that is nearly normal in size, albeit still abnormal in lens pattern.
Co-expression of Abl[Scer\UAS.cFa] enhances the chb[Scer\UAS.T:Avic\GFP] mutant eye phenotype when both transgenes are expressed under the control of Scer\GAL4[GMR.PF]. The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab[1]/Dab[2] females and having Dab[1] as the paternally derived copy of Dab) is decreased by expression of Abl[Scer\UAS.cFa] under the control of Scer\GAL4[elav.PU] to 29%. The rough eye phenotype caused by expression of Hem[Scer\UAS.T:Myr-Src64B] under the control of Scer\GAL4[GMR.PU] is enhanced by co-expression of Abl[Scer\UAS.cFa].
The rough eye phenotype caused by expression of Abl[Scer\UAS.cFa] under the control of Scer\GAL4[GMR.PU] is enhanced by co-expression of Ptp61F[dsRNA.Scer\UAS.WIZ] and is suppressed co-expression of Ptp61F[m.Scer\UAS]. Expression of bskDN.Scer\UAS, has no effect on Scer\GAL4P2.4.Pdf>AblScer\UAS.cFa-induced axonal arborization of the sLNv. Co-expression of chbEP3403 and AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu in embryos results in an increased frequency of axons ectopically crossing the midline compared to embryos expressing either chbEP3403 or AblScer\UAS.cFa singly under the control of Scer\GAL4elav.PLu. Co-expression of chbEP3403 enhances the expressivity of the ISNb axons phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu, although the effect is subtle. The ISNb bypass phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu is reduced by chb4/chb4. Co-expression of roboScer\UAS.cKa, roboΔCC2.Scer\UAS or roboΔCC3.Scer\UAS enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF. Co-expression of leaScer\UAS.cSa enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF. Co-expression of robo3Scer\UAS.cSa enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF. Co-expression of AblScer\UAS.cFa and roboScer\UAS.cKa under the control of Scer\GAL4elav-C155 dramatically increases the frequency of the ISNb bypass phenotype seen when each is expressed alone under the control of Scer\GAL4elav-C155. Co-expression of AblScer\UAS.cFa and roboΔCC3.Scer\UAS under the control of Scer\GAL4elav-C155 dramatically increases the frequency of the ISNb bypass phenotype seen when each is expressed alone under the control of Scer\GAL4elav-C155. Expression of AblScer\UAS.cFa under the control of Scer\GAL4T155 in capt10 mosaic egg chambers alters both the level and distribution of actin filaments in the capt10 mutant cells. In a few egg chambers, the epithelial morphology is profoundly disrupted. The ISNb bypass phenotype of embryos expressing AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu is partially suppressed by LarScer\UAS.cKa. | |||
Xenogenetic Interactions
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Statement Reference | |||
Complementation & Rescue Data
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| Rescues | |||
| Partially rescues | |||
| Fails to rescue | |||
| Comments | The increased bouton number per muscle area that is seen at the larval neuromuscular junction of Abl[4]/Df(3L)st-j7 animals is rescued by expression of Abl[Scer\UAS.cFa] under the control of Scer\GAL4[elav-C155] but is not rescued by expression of Abl[Scer\UAS.cFa] under the control of Scer\GAL4[how-24B]. | ||
Stocks
( 3 ) | |||
| Bloomington | 28993 | ||
Notes on Origin
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External Crossreferences & Linkouts
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Synonyms & Secondary IDs
( 3 ) | |||
| Reported As | |||
| Symbol Synonym | AblScer\UAS.cFa AblUAS.cFa | ||
| Name Synonym | Saccharomyces cerevisiae UAS construct a of Fogerty | ||
| Secondary FlyBase IDs | |||
References
( 14 ) | |||
| Research paper |
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Recent Updates
External Crossreferences & Linkouts