Allele Dmel\AblScer\UAS.cFa
| General Information | |||
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| Symbol | Dmel\AblScer\UAS.cFa | Species | D. melanogaster |
| Name | Saccharomyces cerevisiae UAS construct a of Fogerty | FlyBase ID | FBal0095895 |
| Feature type | allele | Created / Updated | 2006-08-20/2006-08-20 |
| Associated gene | Dmel\Abl | ||
| Allele class | |||
| Mutagen | in vitro construct | regulatory fusion | ||
Nature of the Allele
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| Allele class | |||
| Mutagen | |||
| Mapped Features and Mutations | |||
Type Symbol & Location Additional Notes References | |||
| Associated Sequence Data | |||
| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | ||
| UniProtKB/Swiss-Prot | |||
| UniProtKB/TrEMBL | |||
| Progenitor genotype | |||
| Nature of the lesion | Statement Reference | ||
| Assay mode | |||
| Carried in construct | |||
| Cytology | |||
Phenotypic Data
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Phenotypic Class
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Phenotype Manifest In
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ventral adult lateral neuron & axon, with Scer\GAL4P2.4.Pdf | |||
Detailed Description
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Statement Reference Embryos expressing AblScer\UAS.cFa under the control of Scer\GAL431 have no visible defects in the central nervous system. Flies expressing AblScer\UAS.cFa under the control of Scer\GAL4hs.2sev are viable and fertile and have a mild rough eye phenotype; ommatidia are often fused and mechanosensory bristles are occasionally misplaced. Ommatidia are irregular in shape and size and show an altered trapezoid orientation of photoreceptor cells. The arrangement of the ommatidia is disrupted. Most, but not all, ommatidia contain the correct number of photoreceptor cells. ISNb axons often show a bypass phenotype in embryos expressing AblScer\UAS.cFa under the control of Scer\GAL41407 or Scer\GAL4elav.PLu. When AblScer\UAS.cFa is expressed under the control if Scer\GAL4elav.PLu or Scer\GAL4sca-537.4, the bundles of longitudinal connectives of the medial pathway ectopically crosses the midline, in a dose dependant manner. Expression of AblScer\UAS.cFa under the control of Scer\GAL4hs.PB results in multilayering of the follicle epithelium. Expression of AblScer\UAS.cFa under the control of Scer\GAL4T155 has little effect on follicle cells. Flies expressing AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF have a mild rough eye phenotype. Expression of AblScer\UAS.cFa under the control of Scer\GAL4elav-C155 results in a low level of guidance errors in the ISNb. AblScer\UAS.cFa animals exhibit a ISNb bypass phenotype. Embryos expressing AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu show axons ectopically crossing the midline. In addition, ISNb axons in these embryos show an " ISNb bypass" phenotype, often failing to enter their ventral target domain (muscles 7/6, 13 and 12). Once they have passed their targets, the misguided ISNb axons often make contacts with muscle 12 ("reach-back" phenotype). Overexpression of AblScer\UAS.cFa, under the control of Scer\GAL4P2.4.Pdf, induces a small increase in axonal outgrowth of the sLNv. | |||
Interactions
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Phenotypic Class
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Enhanced by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4elav-C155 has neuroanatomy defective phenotype, enhanceable by roboΔCC3.Scer\UAS AblScer\UAS.cFa, Scer\GAL4elav-C155 has neuroanatomy defective phenotype, enhanceable by roboScer\UAS.cKa | |||
Suppressed by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4elav.PLu has neuroanatomy defective phenotype, suppressible | partially by chb4/chb4 | |||
NOT suppressed by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4P2.4.Pdf has neuroanatomy defective phenotype, non-suppressible by bskDN.Scer\UAS | |||
Enhancer of | |||
Statement Reference AblScer\UAS.cFa is an enhancer of neuroanatomy defective phenotype of Scer\GAL4elav-C155, roboΔCC3.Scer\UAS AblScer\UAS.cFa is an enhancer of neuroanatomy defective phenotype of Scer\GAL4elav-C155, roboScer\UAS.cKa | |||
Suppressor of | |||
Statement Reference Scer\GAL431/AblScer\UAS.cFa is a suppressor | partially of lethal phenotype of Abl1/Df(3L)st-j7, NrtM2 | |||
Phenotype Manifest In
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Enhanced by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4elav-C155 has anterior fascicle phenotype, enhanceable by roboΔCC3.Scer\UAS AblScer\UAS.cFa, Scer\GAL4elav-C155 has anterior fascicle phenotype, enhanceable by roboScer\UAS.cKa | |||
Suppressed by | |||
Statement Reference AblScer\UAS.cFa, LarScer\UAS.cKa has anterior fascicle phenotype, suppressible | partially by Scer\GAL4elav.PLu AblScer\UAS.cFa, Scer\GAL4elav.PLu has anterior fascicle phenotype, suppressible | partially by chb4/chb4 AblScer\UAS.cFa, Scer\GAL4unspecified has anterior fascicle phenotype, suppressible by NScer\UAS.cBa | |||
NOT suppressed by | |||
Statement Reference AblScer\UAS.cFa, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by bskDN.Scer\UAS | |||
Enhancer of | |||
Statement Reference AblScer\UAS.cFa is an enhancer of anterior fascicle phenotype of Scer\GAL4elav-C155, roboΔCC3.Scer\UAS AblScer\UAS.cFa is an enhancer of anterior fascicle phenotype of Scer\GAL4elav-C155, roboScer\UAS.cKa Scer\GAL4unspecified/AblScer\UAS.cFa is an enhancer of anterior fascicle | conditional - heat sensitive phenotype of Nl1N-ts1 | |||
Additional Comments
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Genetic Interactions
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Statement Reference The ISNb bypass phenotype of embryos expressing AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu is partially suppressed by LarScer\UAS.cKa. Expression of AblScer\UAS.cFa under the control of Scer\GAL4T155 in capt10 mosaic egg chambers alters both the level and distribution of actin filaments in the capt10 mutant cells. In a few egg chambers, the epithelial morphology is profoundly disrupted. Co-expression of roboScer\UAS.cKa, roboΔCC2.Scer\UAS or roboΔCC3.Scer\UAS enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF. Co-expression of leaScer\UAS.cSa enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF. Co-expression of robo3Scer\UAS.cSa enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF. Co-expression of AblScer\UAS.cFa and roboScer\UAS.cKa under the control of Scer\GAL4elav-C155 dramatically increases the frequency of the ISNb bypass phenotype seen when each is expressed alone under the control of Scer\GAL4elav-C155. Co-expression of AblScer\UAS.cFa and roboΔCC3.Scer\UAS under the control of Scer\GAL4elav-C155 dramatically increases the frequency of the ISNb bypass phenotype seen when each is expressed alone under the control of Scer\GAL4elav-C155. Co-expression of chbEP3403 and AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu in embryos results in an increased frequency of axons ectopically crossing the midline compared to embryos expressing either chbEP3403 or AblScer\UAS.cFa singly under the control of Scer\GAL4elav.PLu. Co-expression of chbEP3403 enhances the expressivity of the ISNb axons phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu, although the effect is subtle. The ISNb bypass phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4elav.PLu is reduced by chb4/chb4. Expression of bskDN.Scer\UAS, has no effect on Scer\GAL4P2.4.Pdf>AblScer\UAS.cFa-induced axonal arborization of the sLNv. | |||
Xenogenetic Interactions
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Statement Reference | |||
Complementation & Rescue Data
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| Rescues | |||
| Partially rescues | |||
| Comments | |||
Stocks
( 2 ) | |||
| Bloomington | |||
Notes on Origin
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| Discoverer | |||
Synonyms & Secondary IDs
( 3 ) | |||
| Reported As | |||
| Symbol Synonym | AblScer\UAS.cFa AblUAS.cFa | ||
| Name Synonym | Saccharomyces cerevisiae UAS construct a of Fogerty | ||
| Secondary FlyBase IDs | |||
References
( 9 ) | |||
| Research paper |
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Nature of the Allele