FlyBase Allele Report: Dmel\Abl::Hsap\ABL1::Hsap\BCR[P185.UAS]

General Information

Symbol

Dmel\Abl::Hsap\ABL1::Hsap\BCR^P185.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0095898

Feature type

allele

Associated gene

Abl::Hsap\ABL1::Hsap\BCR

Associated Insertion(s)

Carried in Construct

P{UAS-P185}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Fogerty et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-P185}

Cytology

Description

UASt regulatory sequences drive expression of a chimeric construct consisting of Hsap\BCR exon 1 (encoding 426 amino acids), Hsap\ABL1 exon 2 and the first 204 codons of Hsap\ABL1 exon 3 and Abl sequence from codon 47 to the C-terminal end of the Abl protein.

(Fogerty et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Abl::Hsap\ABL1::Hsap\BCR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of chronic myeloid leukemia

CEA

(Fogerty et al., 1999)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

viable, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

visible, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

Phenotype Manifest In

eye, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

interommatidial bristle, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

lens, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

ommatidium, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

presumptive embryonic/larval central nervous system, with Scer\GAL4³¹

(Fogerty et al., 1999)

rhabdomere, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

secondary pigment cell, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

tertiary pigment cell, with Scer\GAL4^hs.2sev

(Fogerty et al., 1999)

Detailed Description

Statement

Reference

Embryos expressing Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} under the control of Scer\GAL4³¹ have defects in the central nervous system. Flies expressing Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} under the control of Scer\GAL4^hs.2sev are viable and fertile and have a severe rough eye phenotype; the eyes are small and bar-shaped with a large reduction in the number of ommatidial facets. The facets are abnormally shaped and vary in size. Multiple misplaced mechanosensory bristles and lens defects are seen. The ommatidia lack one or more retinal cells and/or have elongated or fused rhabdomeres or retinal cells. The secondary and tertiary pigment cells are disorganised.

(Fogerty et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4³¹/Abl::Hsap\ABL1::Hsap\BCR^P185.UAS is a suppressor | partially of lethal phenotype of Abl¹/Df(3L)st-j7, Nrt^M2

(Fogerty et al., 1999)

Scer\GAL4³¹/Abl::Hsap\ABL1::Hsap\BCR^P185.UAS is a suppressor of lethal phenotype of Abl¹/Df(3L)st-j7

(Fogerty et al., 1999)

Other

Statement

Reference

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹, Scer\GAL4³¹ has viable phenotype

(Fogerty et al., 1999)

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹, Scer\GAL4³¹ has visible phenotype

(Fogerty et al., 1999)

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹/Df(3L)st-j7, Scer\GAL4³¹ has visible phenotype

(Fogerty et al., 1999)

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has visible phenotype

(Fogerty et al., 1999)

Phenotype Manifest In

Other

Statement

Reference

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹/Df(3L)st-j7, Scer\GAL4³¹ has eye phenotype

(Fogerty et al., 1999)

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹/Df(3L)st-j7, Scer\GAL4³¹ has ommatidium phenotype

(Fogerty et al., 1999)

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has eye phenotype

(Fogerty et al., 1999)

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has ommatidium phenotype

(Fogerty et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality of Abl¹/Df(3L)st-j7 is rescued by Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} expressed under the control of Scer\GAL4³¹. The rescued flies are variably fertile and have rough eyes. The lethality of Abl¹/Nrt^M2 Df(3L)st-j7 is partially rescued by Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} expressed under the control of Scer\GAL4³¹. The rescued flies are fertile and have rough eyes.

(Fogerty et al., 1999)

Xenogenetic Interactions

Statement

Reference

The lethality of Abl¹/Df(3L)st-j7 is rescued by Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} expressed under the control of Scer\GAL4³¹. The rescued flies are variably fertile and have rough eyes.

The lethality of Abl¹/Nrt^M2 Df(3L)st-j7 is partially rescued by Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} expressed under the control of Scer\GAL4³¹. The rescued flies are fertile and have rough eyes.

(Fogerty et al., 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The part of the chimeric construct derived from human sequences corresponds to the P185 BCR-ABL1 fusion protein found in Philadelphia chromosome positive acute lymphoblastic leukemia.

(Fogerty et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS}

Abl::Hsap\ABL1::Hsap\BCR^P185.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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