Allele Dmel\tkv^CA.Scer\UAS

General Information
Symbol	Dmel\tkv^CA.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0096660
Feature type	allele	Associated gene	Dmel\tkv

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Adachi-Yamada et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A constitutively active form of the short isoform of tkv, in which a Gln residue in the GS domain has been replaced by an acidic residue, is expressed under the control of Scer\UAS regulatory sequences. (Adachi-Yamada et al., 1999)
Carried in construct	P{UAS-tkv.CA} (Tsuda et al., 1999, Niwa et al., 2004, Adachi-Yamada and O'Connor, 2002, Adachi-Yamada et al., 1999, Adachi-Yamada et al., 1999, Jaramillo et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^71B (Adachi-Yamada et al., 1999, Adachi-Yamada et al., 1999)
Phenotype Manifest In
	abdomen, with Scer\GAL4^71B (Adachi-Yamada et al., 1999) mesothoracic extracoxal depressor muscle 66 \| supernumerary, with Scer\GAL4¹¹⁵¹ (Jaramillo et al., 2009) thoracic adult muscle precursor cell, with Scer\GAL4¹¹⁵¹ (Jaramillo et al., 2009) wing, with Scer\GAL4^71B (Adachi-Yamada et al., 1999, Adachi-Yamada et al., 1999) wing disc, with Scer\GAL4^69B (Adachi-Yamada et al., 1999) wing disc, with Scer\GAL4^71B (Adachi-Yamada et al., 1999) wing disc, with Scer\GAL4^Act5C.PI (Adachi-Yamada et al., 1999) wing disc \| somatic clone, with Scer\GAL4^Act5C.PI (Adachi-Yamada and O'Connor, 2002) wing vein, with Scer\GAL4^71B (Adachi-Yamada et al., 1999)
Detailed Description
	Statement Reference Scer\GAL4[1151] driven tkv[CA.Scer\UAS] engenders an approximately two-fold increase in the number of tergal depressor of trochanter (TDT) muscle fibers, although each individual fiber is somewhat smaller than in wild-type muscle. An increase in the number of TDT founder cells is clearly visible by 16 hours after puparium formation (APF) as a result of Scer\GAL4[1151] driven tkv[CA.Scer\UAS] expression. (Jaramillo et al., 2009) When tkv^CA.Scer\UAS clones are made in the wing disc (driven by Scer\GAL4^Act5C.PI) produces clones with a rounded appearance. (Adachi-Yamada and O'Connor, 2002) Normal wing venation is severely distorted when tkv^CA.Scer\UAS is expressed under the control of Scer\GAL4^71B, and extensive production of fragments of vein material is seen. The abdominal cuticle pattern is irregular. The wing phenotype is significantly suppressed by exposure of the larvae to the imidazole compound SB203580 (a p38 inhibitor). The wing phenotype caused by tkv^CA.Scer\UAS expressed under the control of Scer\GAL4^71B is not suppressed by tkv⁷. (Adachi-Yamada et al., 1999) Flies expressing tkv^CA.Scer\UAS under the control of Scer\GAL4^71B often show notching of the wing margin. The notches are located away from the distal tip of the wing and are limited to the more proximal region. Dying cells are distributed throughout the primordium of the proximal wing in wing discs expressing tkv^CA.Scer\UAS under the control of Scer\GAL4^71B but are absent from the distal wing primordium. When tkv^CA.Scer\UAS is expressed mosaically in the wing primordia using Scer\GAL4^Act5C.PI, dying cells are seen in clones in the proximal wing but not in the distal wing. (Adachi-Yamada et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^71B, tkv^CA.Scer\UAS has visible phenotype, suppressible by Df(2L)b82a2 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has visible phenotype, suppressible by Df(2L)C28 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has visible phenotype, suppressible by Scer\GAL4^71B/p38b^a.Scer\UAS (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has visible phenotype, suppressible by Scer\GAL4^71B/p38b^DN.Scer\UAS (Adachi-Yamada et al., 1999)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^69B, tkv^CA.Scer\UAS has wing disc phenotype, suppressible by p38b^a.Scer\UAS/Scer\GAL4^69B (Adachi-Yamada et al., 1999) Scer\GAL4^69B, tkv^CA.Scer\UAS has wing disc phenotype, suppressible by p38b^DN.Scer\UAS/Scer\GAL4^69B (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has abdomen phenotype, suppressible by Scer\GAL4^71B/p38b^a.Scer\UAS (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing disc phenotype, suppressible by hep^r75 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, suppressible by Df(2L)b82a2 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, suppressible by Df(2L)C28 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, suppressible by Scer\GAL4^71B/p38b^a.Scer\UAS (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, suppressible by Scer\GAL4^71B/p38b^DN.Scer\UAS (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing vein phenotype, suppressible by Df(2L)b82a2 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing vein phenotype, suppressible by Df(2L)C28 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing vein phenotype, suppressible by Scer\GAL4^71B/p38b^a.Scer\UAS (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing vein phenotype, suppressible by Scer\GAL4^71B/p38b^DN.Scer\UAS (Adachi-Yamada et al., 1999)
NOT suppressed by
Statement Reference Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, non-suppressible by bsk^DN.Scer\UAS/Scer\GAL4^71B (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, non-suppressible by Df(2L)flp147E (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, non-suppressible by Df(3R)crb-F89-4 (Adachi-Yamada et al., 1999) Scer\GAL4^71B, tkv^CA.Scer\UAS has wing phenotype, non-suppressible by dpp^d8 (Adachi-Yamada et al., 1999)
Enhancer of
Statement Reference tkv^CA.Scer\UAS/Scer\GAL4^hs.2sev is an enhancer of ventral thoracic disc phenotype of Scer\GAL4^hs.2sev, ey^Scer\UAS.cHa (Niwa et al., 2004) tkv^CA.Scer\UAS/Scer\GAL4^hs.2sev is an enhancer of wing disc phenotype of Scer\GAL4^hs.2sev, ey^Scer\UAS.cHa (Niwa et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Coexpression of ey^Scer\UAS.cHa and tkv^CA.Scer\UAS under control of Scer\GAL4^hs.2sev results in large ectopic "eyes" (using antibodies against elav to visualize eye differentiation) in both wing and leg discs in regions far from the anterior-posterior compartment boundary. (Niwa et al., 2004) Suppression by Df(2L)b82a2 or p38b^DN.Scer\UAS (expressed under the control of Scer\GAL4^71B) of the wing phenotype caused by tkv^CA.Scer\UAS expressed under the control of Scer\GAL4^71B is reduced if the flies are also coexpressing p38b^Scer\UAS.cAa. The wing disc is overgrown if tkv^CA.Scer\UAS is expressed under the control of Scer\GAL4^69B. This phenotype is strongly suppressed by coexpression of either p38b^a.Scer\UAS or p38b^DN.Scer\UAS. (Adachi-Yamada et al., 1999) The cell death seen in the wing discs of larvae expressing tkv^CA.Scer\UAS under the control of Scer\GAL4^71B is suppressed if the larvae are also hemizygous for hep^r75. (Adachi-Yamada et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	36537 w^*; P{UAS-tkv.CA}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	tkv^CA.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Jaramillo et al., 2009, Development 136(7): 1105--1113 Crossveinless and the TGF{beta} pathway regulate fiber number in the Drosophila adult jump muscle. [FBrf0207556] Niwa et al., 2004, Nat. Genet. 36(3): 293--297 A conserved developmental program for sensory organ formation in Drosophila melanogaster. [FBrf0175004] Adachi-Yamada and O'Connor, 2002, Dev. Biol. 251(1): 74--90 Morphogenetic apoptosis: a mechanism for correcting discontinuities in morphogen gradients. [FBrf0151891] Adachi-Yamada et al., 1999, Mol. Cell. Biol. 19(3): 2322--2329 p38 mitogen-activated protein kinase can be involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis. [FBrf0106118] Adachi-Yamada et al., 1999, Nature 400(6740): 166--169 Distortion of proximodistal information causes JNK-dependent apoptosis in Drosophila wing. [FBrf0108559] Tsuda et al., 1999, Nature 400(6741): 276--280 The cell-surface proteoglycan Dally regulates Wingless signalling in Drosophila. [FBrf0110960]

Allele Dmel\tkvCA.Scer\UAS

Allele Dmel\tkv^CA.Scer\UAS