Allele Dmel\pros^Scer\UAS.cMa

General Information
Symbol	Dmel\pros^Scer\UAS.cMa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Matsuzaki	FlyBase ID	FBal0096724
Feature type	allele	Associated gene	Dmel\pros

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Manning and Doe, 1999, Reddy and Rodrigues, 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of full length pros. (Manning and Doe, 1999) Scer\UAS regulatory sequences drive pros expression. (Reddy and Rodrigues, 1999)
Carried in construct	P{UAS-pros.17K-2} (Liu et al., 2002, Mettler et al., 2006, Li and Vaessin, 2000, Kumar and Moses, 2001, Manning and Doe, 1999, Reddy and Rodrigues, 1999, Ragone et al., 2001, Sen et al., 2003, Kumar and Moses, 2001, Bello et al., 2006, Hartl et al., 2011, Andrews et al., 2009, Kurusu et al., 2009, Cabernard and Doe, 2009, Colonques et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) mitotic cell cycle defective, with Scer\GAL4^en-e16E (Mettler et al., 2006) mitotic cell cycle defective \| larval stage, with Scer\GAL4^hs.PB (Colonques et al., 2011) visible, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) visible, with Scer\GAL4^sca-P309 (Reddy and Rodrigues, 1999)
Phenotype Manifest In
	antenna, with Scer\GAL4^sca-P309 (Sen et al., 2003) embryonic/larval brain, with Scer\GAL4^hs.PB (Colonques et al., 2011) embryonic epidermis, with Scer\GAL4^Kr.PM (Li and Vaessin, 2000) interommatidial bristle, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) interommatidial bristle & tormogen cell, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) interommatidial bristle & trichogen cell, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) larval outer optic anlage, with Scer\GAL4^hs.PB (Colonques et al., 2011) mesoderm, with Scer\GAL4^Kr.PM (Li and Vaessin, 2000) microchaeta & antennal segment 3, with Scer\GAL4^sca-P309 (Sen et al., 2003) morphogenetic furrow, with Scer\GAL4^dpp.blk1 (Kumar and Moses, 2001) neuroblast \| larval stage, with Scer\GAL4^wor.PA (Cabernard and Doe, 2009) neuroblast NB7-3, with Scer\GAL4^en-e16E (Mettler et al., 2006) scutum & external sensory organ & tormogen cell, with Scer\GAL4^sca-P309 (Reddy and Rodrigues, 1999) scutum & external sensory organ & trichogen cell, with Scer\GAL4^sca-P309 (Reddy and Rodrigues, 1999) scutum & macrochaeta, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) scutum & macrochaeta & tormogen cell, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) scutum & macrochaeta & trichogen cell, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) scutum & microchaeta, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) scutum & microchaeta & tormogen cell, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) scutum & microchaeta & trichogen cell, with Scer\GAL4^sca-109-68 (Manning and Doe, 1999) ventral nerve cord, with Scer\GAL4^Kr.PM (Li and Vaessin, 2000)
Detailed Description
	Statement Reference Larvae expressing pros[Scer\UAS.cMa] under the control of pros[Scer\UAS.cMa] (using heat shock) show a marked decrease in the number of mitotically active cells in the brain compared to controls. This reduction is seen both in the outer proliferation centre and in the central brain. (Colonques et al., 2011) Expression of pros[Scer\UAS.cMa] in the neuroblasts under the control of Scer\GAL4[wor.PA] results in depletion of larval neuroblast numbers. (Cabernard and Doe, 2009) Ectopic expression of pros^Scer\UAS.cMa, under the control of Scer\GAL4^en-e16E causes a precocious stop in cell division within the NB7-3 lineage. (Mettler et al., 2006) When pros^Scer\UAS.cMa is driven by Scer\GAL4^sca-P309, a striking reduction in external structures on the adult antenna is seen. No increase in the number of glia or neurons is seen. (Sen et al., 2003) Expression of pros^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 inhibits furrow reincarnation in the eye disc. (Kumar and Moses, 2001) When pros^Scer\UAS.cMa is driven by Scer\GAL4^eg-Mz360 or Scer\GAL4^sca-4512 no increase in NGB6-4t-derived glial cells is seen. (Ragone et al., 2001) Expression of pros^Scer\UAS.cMa under the control of Scer\GAL4^Kr.PM in embryos results in thinning and disruptions in the ventral nerve cord, and reduced epidermal and mesodermal structures. There is a strong reduction in BrdU labeling in these embryos compared to wild type. (Li and Vaessin, 2000) Animals expressing pros^Scer\UAS.cMa under the control of Scer\GAL4^sca-109-68 die as pharate adults and are virtually bald, having less than 1% of the normal external sense organ bristles in the notum and eye. The external sense organs lack both the bristle and socket. (Manning and Doe, 1999) Flies expressing pros^Scer\UAS.cMa under the control of Scer\GAL4^sca-P309 have a bald notum where both the shaft and socket of the bristles are absent. (Reddy and Rodrigues, 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa has cell cycle defective \| larval stage phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa (Kurusu et al., 2009, Kurusu et al., 2009)
Suppressor of
Statement Reference pros^Scer\UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of neuroanatomy defective \| somatic clone phenotype of brat¹¹ (Bello et al., 2006) Scer\GAL4^Act.PU/pros^Scer\UAS.cMa is a suppressor \| partially \| somatic clone of neuroanatomy defective \| somatic clone \| adult stage phenotype of mir-279^S096207 (Hartl et al., 2011) Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa is a suppressor of cell cycle defective \| larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa (Kurusu et al., 2009, Kurusu et al., 2009) Scer\GAL4^{elav.Switch.PO}/pros^Scer\UAS.cMa is a suppressor of cell cycle defective \| larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, tll^Scer\UAS.cKa (Kurusu et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa has brain \| larval stage phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa (Kurusu et al., 2009, Kurusu et al., 2009)
Suppressor of
Statement Reference pros^Scer\UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of embryonic/larval brain \| somatic clone phenotype of brat¹¹ (Bello et al., 2006) Scer\GAL4^Act.PU/pros^Scer\UAS.cMa is a suppressor \| partially \| somatic clone of carbon dioxide sensitive neuron \| ectopic \| somatic clone phenotype of mir-279^S096207 (Hartl et al., 2011) Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa is a suppressor of brain \| larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, pros^Scer\UAS.cMa (Kurusu et al., 2009, Kurusu et al., 2009) Scer\GAL4^{elav.Switch.PO}/pros^Scer\UAS.cMa is a suppressor of brain \| larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, tll^Scer\UAS.cKa (Kurusu et al., 2009) Scer\GAL4^wor.PA/pros^Scer\UAS.cMa is a suppressor of neuroblast \| supernumerary phenotype of mud³/mud⁴ (Cabernard and Doe, 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of pros[Scer\UAS.cMa] under the control of Scer\GAL4[Act.PU] suppresses the formation of ectopic CO[[2]] neurons on the maxillary palp caused by mir-279[S096207] clones in 37% of animals. (Hartl et al., 2011) Co-overexpression of pros[Scer\UAS.cMa] and tll[Scer\UAS.cKa] by Scer\GAL4[elav.Switch.PO] rescues the tll[Scer\UAS.cKa]-induced brain hyperplasia in third instar larvae. (Kurusu et al., 2009) Expression of pros^Scer\UAS.cMa, under the control of Scer\GAL4^αTub84B.PL, in brat¹¹ mutant larval clones significantly suppresses the overproliferation phenotype in these clones in the central larval brain. Over 90% of Scer\GAL4^αTub84B.PL>pros^Scer\UAS.cMa, brat¹¹ clones in the central brain have <100 cells, while over 80% of brat¹¹ clones have 200-1000 cells. (Bello et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^Act.PU/pros^Scer\UAS.cMa rescues pros^IG2227 (Hartl et al., 2011)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	F. Matsukazi F. Matsuzaki

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	pros^Scer\UAS.cMa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Matsuzaki (Manning and Doe, 1999)
Secondary FlyBase IDs

References ( 15 )
Research paper	Colonques et al., 2011, PLoS ONE 6(4): e19342 A Transient Expression of Prospero Promotes Cell Cycle Exit of Drosophila Postembryonic Neurons through the Regulation of Dacapo. [FBrf0213608] Hartl et al., 2011, J. Neurosci. 31(44): 15660--15673 A New Prospero and microRNA-279 Pathway Restricts CO2 Receptor Neuron Formation. [FBrf0216631] Andrews et al., 2009, EMBO Rep. 10(6): 636--641 Sequoia regulates cell fate decisions in the external sensory organs of adult Drosophila. [FBrf0208172] Cabernard and Doe, 2009, Dev. Cell 17(1): 134--141 Apical/basal spindle orientation is required for neuroblast homeostasis and neuronal differentiation in Drosophila. [FBrf0208429] Kurusu et al., 2009, Dev. Biol. 326(1): 224--236 A conserved nuclear receptor, Tailless, is required for efficient proliferation and prolonged maintenance of mushroom body progenitors in the Drosophila brain. [FBrf0207181] Bello et al., 2006, Development 133(14): 2639--2648 The brain tumor gene negatively regulates neural progenitor cell proliferation in the larval central brain of Drosophila. [FBrf0194745] Mettler et al., 2006, Development 133(3): 429--437 Timing of identity: spatiotemporal regulation of hunchback in neuroblast lineages of Drosophila by Seven-up and Prospero. [FBrf0190310] Sen et al., 2003, Dev. Biol. 254(1): 79--92 Combinatorial expression of Prospero, Seven-up, and Elav identifies progenitor cell types during sense-organ differentiation in the Drosophila antenna. [FBrf0158845] Liu et al., 2002, Mech. Dev. 112(1-2): 25--36 Transcription of the Drosophila CKI gene dacapo is regulated by a modular array of cis-regulatory sequences. [FBrf0144993] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Ragone et al., 2001, Dev. Biol. 235(1): 74--85 A novel mode of asymmetric division identifies the fly neuroglioblast 6-4T. [FBrf0137378] Li and Vaessin, 2000, Genes Dev. 14(2): 147--151 Pan-neural prospero terminates cell proliferation during Drosophila neurogenesis. [FBrf0125193] Manning and Doe, 1999, Development 126(10): 2063--2071 Prospero distinguishes sibling cell fate without asymmetric localization in the Drosophila adult external sense organ lineage. [FBrf0108428] Reddy and Rodrigues, 1999, Development 126(10): 2083--2092 Sibling cell fate in the Drosophila adult external sense organ lineage is specified by prospero function, which is regulated by Numb and Notch. [FBrf0108429]

Allele Dmel\prosScer\UAS.cMa

Allele Dmel\pros^Scer\UAS.cMa