Allele Dmel\p38b^Scer\UAS.cAa

General Information
Symbol	Dmel\p38b^Scer\UAS.cAa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Adachi-Yamada	FlyBase ID	FBal0096730
Feature type	allele	Associated gene	Dmel\p38b

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Adachi-Yamada et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: p38b is expressed under the control of Scer\UAS regulatory sequences. (Adachi-Yamada et al., 1999)
Carried in construct	P{UAS-p38b.A} (Adachi-Yamada et al., 1999, Cha et al., 2003, Chen et al., 2010, Vrailas-Mortimer et al., 2011, Shinzawa et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	heat stress response defective, with Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) immune response defective \| adult stage, with Scer\GAL4^da.G32 (Shinzawa et al., 2009) immune response defective \| adult stage, with Scer\GAL4^Pxn.PS (Shinzawa et al., 2009) long lived, with Scer\GAL4^DJ757 (Vrailas-Mortimer et al., 2011) long lived, with Scer\GAL4^EDTP-DJ694 (Vrailas-Mortimer et al., 2011) long lived, with Scer\GAL4^how-24B (Vrailas-Mortimer et al., 2011) long lived, with Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) long lived, with Scer\GAL4^Mhc.PW (Vrailas-Mortimer et al., 2011) long lived, with Scer\GAL4^Mhc.PW, Scer\GAL80^ts.αTub84B (Vrailas-Mortimer et al., 2011) oxidative stress response defective, with Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011) oxidative stress response defective, with Scer\GAL4^p38b-83 (Vrailas-Mortimer et al., 2011) starvation stress response defective, with Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011)
Phenotype Manifest In

Detailed Description
	Statement Reference Flies expressing p38b[Scer\UAS.cAa] in muscles throughout their lifetimes under the control of either Scer\GAL4[Mhc.PW], Scer\GAL4[Mef2.PR], Scer\GAL4[how-24B], Scer\GAL4[EDTP-DJ694] or Scer\GAL4[DJ757] live significantly longer than controls. Pan-neuronal expression under the control of Scer\GAL4[elav-C155] has no effect on lifespan. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mhc.PW], and restricted to post-eclosion stages using Scer\GAL80[ts.αTub84B], results in a modest increase in lifespan compared to controls. Restricting p38b[Scer\UAS.cAa] expression to adult stage neurons under the control of Scer\GAL4[elav-C155] has no effect on lifespan. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mhc.PW] suppresses the lethality that is usually seen in response to heat and dry starvation. No suppression of the phenotype occurs when p38b[Scer\UAS.cAa] is expressed in neurons under the control of Scer\GAL4[elav-C155]. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] confers additional resistance to hydrogen peroxide-induced oxidative stress compared to wild type. A similar phenotype is seen when p38b[Scer\UAS.cAa] is expressed in the p38Kb expression domain under the control of Scer\GAL4[p38b-83]. (Vrailas-Mortimer et al., 2011) Expression of p38b[Scer\UAS.cAa] using Scer\GAL4[da.G32] or Scer\GAL4[Pxn.PS] suppresses Salmonella-induced lethality without a subsequent reduction in bacterial load. The tolerance of p38b[Scer\UAS.cAa] Scer\GAL4[da.G32] flies to Salmonella infection is abolished if the engulfing function of hemocytes is blocked through the introduction of non-digestible latex beads. Following infection with Salmonella, Scer\GAL4[Pxn.PS], p38b[Scer\UAS.cAa] flies show an increase in intracellular persistence of Salmonella. This contrasts with controls, in which the intracellular Salmonella count clearly decreases during infection. (Shinzawa et al., 2009) Suppression by Df(2L)b82a2 or p38b^DN.Scer\UAS of the wing phenotype caused by tkv^CA.Scer\UAS expressed under the control of Scer\GAL4^71B is reduced if the flies are also coexpressing p38b^Scer\UAS.cAa. (Adachi-Yamada et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^Mef2.PR, p38b^Scer\UAS.cAa has long lived phenotype, suppressible by Sod2^{dsRNA.Scer\UAS.cKa}/Scer\GAL4^Mef2.PR (Vrailas-Mortimer et al., 2011)
Suppressor of
Statement Reference p38b^Scer\UAS.cAa, Scer\GAL4^sca-537.4 is a suppressor of visible phenotype of Rala^{S25N.cMa.Scer\UAS}, Scer\GAL4^sca-537.4 (Balakireva et al., 2006) p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor \| partially of oxidative stress response defective phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011) p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor \| partially of short lived phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011) p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor of gravitaxis defective phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011) p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor of locomotor behavior defective phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011) p38b^Scer\UAS.cAa/Scer\GAL4^Mef2.PR is a suppressor of partially lethal - majority die phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^elav-C155/p38b^Scer\UAS.cAa is a non-suppressor of gravitaxis defective phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011) Scer\GAL4^elav-C155/p38b^Scer\UAS.cAa is a non-suppressor of locomotor behavior defective phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011) Scer\GAL4^elav-C155/p38b^Scer\UAS.cAa is a non-suppressor of short lived phenotype of Mpk2¹, p38b^Δ25 (Vrailas-Mortimer et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference p38b^Scer\UAS.cAa, Scer\GAL4^sca-537.4 is a suppressor of macrochaeta phenotype of Rala^{S25N.cMa.Scer\UAS}, Scer\GAL4^sca-537.4 (Balakireva et al., 2006) p38b^Scer\UAS.cAa, Scer\GAL4^sca-537.4 is a suppressor of microchaeta phenotype of Rala^{S25N.cMa.Scer\UAS}, Scer\GAL4^sca-537.4 (Balakireva et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] significantly rescues the reduction in lifespan seen in p38b[Δ25] Mpk2[1] double mutants. No rescue is seen when p38b[Scer\UAS.cAa] is expressed in neurons under the control of Scer\GAL4[elav-C155]. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] suppresses the geotaxis and walking defects seen in p38b[Δ25] Mpk2[1] double mutant females. No suppression is seen when p38b[Scer\UAS.cAa] is expressed pan-neuronally under the control of Scer\GAL4[elav-C155]. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] significantly rescues the increased sensitivity to hydrogen peroxide-induced oxidative stress seen in p38b[Δ25] Mpk2[1] double mutants. Expression of p38b[Scer\UAS.cAa] in muscles under the control of Scer\GAL4[Mef2.PR] completely rescues the reduced viability seen in p38b[Δ25] Mpk2[1] double mutants. Expression of Sod2[dsRNA.Scer\UAS.cKa] suppresses the lifespan extension phenotype seen when p38b[Scer\UAS.cAa] is expressed in muscles under the control of Scer\GAL4[Mef2.PR]. (Vrailas-Mortimer et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	p38b^Scer\UAS.cAa/Scer\GAL4^da.G32 rescues p38b^ex9 (Shinzawa et al., 2009)
Comments	Expression of p38b[Scer\UAS.cAa] under the control of Scer\GAL4[da.G32] rescues the survival rate of Mpk2[13] p38b[156A] adults infected with either Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana to a level close to wild type. (Chen et al., 2010) Scer\GAL4[da.G32]-mediated expression of p38b[Scer\UAS.cAa] rescues the Salmonella-induced mortality of p38b[ex9] flies. (Shinzawa et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	p38b^Scer\UAS.cAa p38b^WT (Balakireva et al., 2006)
Name Synonym	Saccharomyces cerevisiae UAS construct a of Adachi-Yamada (Adachi-Yamada et al., 1999)
Secondary FlyBase IDs

References ( 6 )
Research paper	Vrailas-Mortimer et al., 2011, Dev. Cell 21(4): 783--795 A Muscle-Specific p38 MAPK/Mef2/MnSOD Pathway Regulates Stress, Motor Function, and Life Span in Drosophila. [FBrf0216446] Chen et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(48): 20774--20779 Participation of the p38 pathway in Drosophila host defense against pathogenic bacteria and fungi. [FBrf0212390] Shinzawa et al., 2009, Cell Host Microbe 6(3): 244--252 p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila. [FBrf0208827] Balakireva et al., 2006, Mol. Cell. Biol. 26(23): 8953--8963 The Ral/exocyst effector complex counters c-Jun N-terminal kinase-dependent apoptosis in Drosophila melanogaster. [FBrf0194568] Cha et al., 2003, Mol. Cell. Biol. 23(22): 7982--7991 Discrete functions of TRAF1 and TRAF2 in Drosophila melanogaster mediated by c-Jun N-terminal kinase and NF-kappaB-dependent signaling pathways. [FBrf0167884] Adachi-Yamada et al., 1999, Mol. Cell. Biol. 19(3): 2322--2329 p38 mitogen-activated protein kinase can be involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis. [FBrf0106118]

Allele Dmel\p38bScer\UAS.cAa

Allele Dmel\p38b^Scer\UAS.cAa