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General Information
Symbol
Dmel\witB11
Species
D. melanogaster
Name
FlyBase ID
FBal0096772
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    C4067867T

    Reported nucleotide change:

    C684T

    Amino acid change:

    Q92term | wit-PA; Q92term | wit-PB

    Reported amino acid change:

    Q92term

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    witB11/witA12 (but not witB11/+) third instar larvae display synaptic undergrowth with fewer mature and satellite boutons at the NMJ than controls.

    witB11/witA12 transheterozygotes do not reach adulthood and present significant changes in the third instar larval neuromuscular junction compared to controls: a decrease in the number, but not in the area, of synaptic boutons; a significant increase in the active zone density; a significant increase in the total postsynaptic density length; a significant increase in the ruffle density at the synapse; and significant decreases in the subsynaptic reticulum length, but not density.

    Mutants show loss of expression of P{FMRFa-EGFP.Tv}, a marker for the six Ap4 neurons in the developing ventral nerve cord, and loss of expression of eya, which identifies the four Ap cluster neurons.

    witA12/witB11 mutant third instar larvae do display synaptic undergrowth (reduced number of boutons) at neuromuscular junctions compared to controls.

    witA12/witB11 mutants show a significant reduction in bouton number at neuromuscular junction 4.

    witA12/witB11 third instar larvae show a significant decrease in bouton number and satellite bouton number at the neuromuscular junction compared to controls.

    witB11/witA12 larvae show a reduction in synaptic terminal size at the neuromuscular junction (NMJ) compared to wild type. The frequency and amplitude of the spontaneous miniature excitatory junctional potential (EJP) at the NMJ are decreased compared to wild type.

    witA12/witB11 animals display a 46% reduction in bouton number at neuromuscular junction 4.

    witA12/witB11 transheterozygotes exhibit approximately half the number of synaptic boutons as found in wild-type terminals.

    witA12/witB11 third instar larvae display small neuromuscular junctions.

    witA12/witB11 larvae exhibit synaptic undergrowth.

    aCC/RP2 synaptic current amplitude in witA12/witB11 mutants does not differ from wild-type.

    witA12/witB11 larvae show a decrease in the number of type 1b boutons at muscle 13 compared to wild type.

    The average total bouton number at the neuromuscular junction is decreased in witA12/witB11 larvae compared to controls.

    witA12/witB11 mutants exhibit a dramatic reduction in bouton number (64%), branching (80%), and synaptic area (64%).

    witA12/witB11 trans-heterozygous neuromuscular junctions exhibit very low EJP amplitudes and low quantal content, but maintain wild-type level mEJP amplitudes.

    Mutant stage 13-14 embryos contain the normal number of crystal cells per embryo.

    At 25oC less than 0.5% of witA12/witB11 animals escape from the pupal case.

    witA12/witB11 larvae show a significant decrease in bouton number at the neuromuscular junction.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    NOT Enhanced by
    Suppressed by
    NOT suppressed by
    Enhancer of
    Statement
    Reference
    NOT Enhancer of
    Statement
    Reference
    Suppressor of
    NOT Suppressor of
    Other
    Phenotype Manifest In
    Enhanced by
    NOT Enhanced by
    Statement
    Reference

    witB11/witA12 has synapse phenotype, non-enhanceable by WASpGD1559/Scer\GAL4C57

    Suppressed by
    NOT suppressed by
    Statement
    Reference

    witB11/witA12 has synapse phenotype, non-suppressible by WASpGD1559/Scer\GAL4C57

    Enhancer of
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Statement
    Reference

    witB11/wit[+] is a non-suppressor of eye disc phenotype of spin10403

    witB11/wit[+] is a non-suppressor of glial cell phenotype of spin10403

    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Expressing Rac1N17.UAS under the control of Scer\GAL4elav-C155 in a witA12 background results in significantly fewer satellite boutons, but a similar number of mature synaptic boutons as controls at the NMJ of third instar larvae. The number of both bouton types is significantly reduced when expression is driven in a witA12/witB11 background.

    mtgoe02963, witB11 double heterozygotes eclose at a lower than expected mendelian ratio from cross between single heterozygotes.

    The third instar larval neuromuscular junction defects observed in witB11/witA12 transheterozygotes is not suppressed or enhanced by either Nrx-1273 or Nlg1Δ46 homozygosity.

    The third instar larval neuromuscular junctions of individuals that both express Nrx-1Scer\UAS.cLa under the control of Scer\GAL4elav.PU and are witB11/witA12 transheterozygous show a decrease in the number of synaptic boutons similar to what is observed in witB11/witA12 transheterozygotes, rather than the increase induced by the expression of Nrx-1Scer\UAS.cLa.

    Syx473/+;witB11/+ double heterozygotic third instar larvae do not show significant differences in bouton number at the neuromuscular junction compared to wild type or single heterozygotes.

    witA12/witB11 fully suppresses the increase in mature boutons and branch number seen at the neuromuscular junction of Fmr1Δ113M/+ third instar larvae (branch number is significantly higher in witA12/witB11 larvae than Fmr1Δ113M/+;witA12/witB11 larvae).

    Introducing a single copy of witA12 into the cpxSH1 mutant background also rescues the synaptic overgrowth but not the elevated minis, combination with witA12/witB11 reduces the number of NMJ boutons in cpxSH1 mutants even further, below the control levels and is comparable to that observed in witA12/witB11 mutants alone.

    witA12/witB11 Acsl05847/AcslKO double mutants show a significant reduction in bouton number at neuromuscular junction 4, similar to witA12/witB11 mutants.

    Larvae that are heterozygous for both Neto36 and witB11 have a similar number of boutons to wild type.

    witA12 spartin1/witB11 Df(3R)110 double mutant larvae show a significant decrease in bouton number and satellite bouton number at the neuromuscular junction compared to controls, as occurs in witA12/witB11 single mutant larvae.

    Expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU slightly, but significantly, enhances the reduction in synaptic terminal size at the neuromuscular junction seen in witB11/witA12 larvae.

    Expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU has no effect on the size of the evoked excitatory junctional potential (EJP) at the neuromuscular junction in witB11/witA12 larvae.

    Expression of twitScer\UAS.cKa under the control of Scer\GAL4elav.PU has no effect on miniature EJP (mEJP) amplitude at the neuromuscular junction in witB11/witA12 larvae, while mEJP frequency is slightly increased.

    witA12/witB11 suppresses the increase in average bouton number at the neuromuscular junction which is seen in larvae expressing Snx163A.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav-C155.

    The glial overmigration phenotype seen in spin10403 eye discs is not suppressed if the animals are also heterozygous for witB11.

    WASp1/Df(3R)3450 partially suppresses the small neuromuscular junction phenotype seen in witA12/witB11 mutants.

    Expression of Cip4NIG.15015R or WASpGD1559 post-synaptically (under the control of Scer\GAL4C57) in witA12/witB11 larvae does not affect overall bouton number or satellite bouton formation.

    A witA12 heterozygous background can completely reverse the increase in total bouton number and satellite bouton formation in larvae expressing either Cip4NIG.15015R or WASpGD1559 post-synaptically (under the control of Scer\GAL4C57).

    Post-synaptic expression of DysdsRNA.mub.Scer\UAS under the control of Scer\GAL4eve.RN2 in a witA12/witB11 background increases the synaptic current as in a wild-type background.

    A witA12/witB11 transheterozygous background suppresses the synaptic overgrowth and increased bouton number phenotypes found in nwk1 homozygous third instar larvae, instead the larvae exhibit reduced synaptic growth and fewer boutons than wild type controls.

    spictmut neuromuscular junction overgrowth phenotypes are fully suppressed in witA12/witB11 mutants. The synaptic undergrowth phenotypes in larvae homozygous for spictmut in a witA12/witB11 background are indistinguishable from that of witA12/witB11 mutants alone.

    Expression of DysdsRNA.NH2.Scer\UAS postsynaptically (under the control of Scer\GAL4G14) in witA12/witB11 trans-heterozygous neuromuscular junctions results in similar EJP amplitudes and quantal content as in witA12/witB11 mutants.

    Co-expression of tkv::witwit-EC.tkv-IC.Ubi-p63E and tkv::wittkv-EC.wit-IC.Ubi-p63E rescues the neuromuscular junction size in witHA3/witB11 animals.

    At 25oC ~80% of witA12/witB11, FmrfScer\UAS.cMa (driven by Scer\GAL4dimm-929) animals can partially escape the pupal case. At 28oC about 30% of females fully eclose, and some inflate their wings. While these animals move fairly well they usually die within several days without producing progeny. No rescue is seen in the size of the synapse. Nor is the primary defect in synaptic transmission rescued.

    spin10403/spink09905 animals which are also mutant for witA12/witB11 show a decrease in bouton number.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
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    Mutant
    Wild-type
    Stocks (2)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    References (45)