FB2025_01 , released February 20, 2025
Allele: Dmel\jing22F3
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General Information
Symbol
Dmel\jing22F3
Species
D. melanogaster
Name
FlyBase ID
FBal0096800
Feature type
allele
Associated gene
Dmel\jing
Associated Insertion(s)
Carried in Construct
Also Known As
22F3
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Liu and Montell, 1999)
Progenitor genotype
Cytology
Description

Coordinates of amino acid replacement are numbered according to GenBank accession AAK49526.1.

(Culi et al., 2006)

Amino acid replacement: K664term.

(Culi et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:6,616,413..6,616,413 [+]
Nucleotide change:

A6616413T

Amino acid change:

K664term | jing-PD; K664term | jing-PH; K922term | jing-PI; K664term | jing-PJ

Reported amino acid change:

K664term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Culi et al., 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous clones in the abdomen show a strong decrease in pigmentation.

      Bristles in homozygous clones in the femur show loss of bracts, suggesting a transformation to a more proximal fate. Internalised vesicles that seem to have detached from the leg cuticle are also seen.

      Homozygous clones in the wing blade are generally associated with a decrease or absence of wing vein differentiation. this phenotype is stronger if the clone affects both the dorsal and ventral aspects of the same vein. In addition, some clones adjacent to, but not including a longitudinal vein induce the nonautonomous elimination of that vein. Occasionally, ectopic vein differentiation is seen abutting a homozygous clones.

      Homozygous clones in the alula cause a reduction in its size and its fusion with the main portion of the wing blade. The clones may also segregate from the cuticle and cause an internal vesicle.

      (Culi et al., 2006)

      The border cells may fail to migrate in egg chambers containing homozygous somatic clones. In mosaic egg chambers in which all of the border cells are heterozygous, they migrate normally. In mosaic egg chambers in which all border cells are homozygous, migration of the border cells fails. Border cell clusters composed of a mixture of homozygous and heterozygous cells show several different types of behaviour. Clusters containing a single heterozygous cell fail to migrate, although the heterozygous cell typically moves to the front of the cluster. Clusters containing a single homozygous cell migrate normally. Clusters containing two or more heterozygous cells frequently split into groups, with the heterozygous cells detaching from the homozygous cells and migrating partway towards the oocyte. In other cases, mixed clusters stay together as one group and migrate partway. In a few cases the border cell cluster becomes very extended, with the heterozygous cells migrating in a line, and the homozygous cells trailing behind.

      (Liu and Montell, 2001)

      Homozygous clones induced in the follicle cells result in defects in border cell migration; the border cells fail to initiate migration and remain at or near the anterior tip of the egg chamber even in stage 10.

      (Liu and Montell, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      jing[+]/jing22F3 is an enhancer of visible | dominant | male phenotype of hthk1

      (Culi et al., 2006)

      jing[+]/jing22F3 is an enhancer of visible | dominant phenotype of Pc15

      (Culi et al., 2006)
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      jing22F3 has border follicle cell | somatic clone phenotype, non-suppressible by slbohs.PM

      (Liu and Montell, 2001)
      Enhancer of
      Statement
      Reference

      jing[+]/jing22F3 is an enhancer of adult abdominal segment 4 | male phenotype of hthk1

      (Culi et al., 2006)

      jing[+]/jing22F3 is an enhancer of wing phenotype of Pc15

      (Culi et al., 2006)

      jing[+]/jing22F3 is an enhancer of haltere | ectopic phenotype of Pc15

      (Culi et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The partial transformation of wing to haltere seen in Pc15 heterozygotes is enhanced by jing22F3/+.

      Df(2R)vg-D, AsxXF23, Pcl15/+ flies have almost normal wings. If the flies also carry jing22F3, the arrangement of wing bristles at the posterior wing margin becomes disorganised and there is an increase in the appearance of cuticle patches containing a high density of small trichomes, indicative of a partial wing-to-haltere transformation.

      (Culi et al., 2006)

      The border cells fail to migrate in the slbo01310 jing22F3 double mutant background, although the border cells are still present. Expression of slbohs.PM does not rescue the border cell migration defect in jing22F3 mosaic egg chambers.

      (Liu and Montell, 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      jingrH623

      (Liu and Montell, 2001)

      jingk02002

      (Liu and Montell, 2001)

      jingk03404

      (Liu and Montell, 2001)
      Rescued by

      jing22F3 is rescued by jinghs.PL

      (Liu and Montell, 2001)
      Comments

      Border cell migration is rescued in jing22F3 mosaic egg chambers when jinghs.PL is expressed using a 1 hour heat shock (and then the flies are kept at 18oC for 20 hours to allow migration to occur). Partial migration is seen even in the absence of heat shock.

      (Liu and Montell, 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a mutation that causes defects in border cell migration when homozygous clones are induced in the follicle cells.

      (Liu and Montell, 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      22F3
      (Liu and Montell, 2001, Liu and Montell, 1999)
      jing22F3
      (Kang et al., 2022, Nair et al., 2013, Culi et al., 2006)
      l(2)22F322F3
      Name Synonyms
      Secondary FlyBase IDs
        References (5)