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General Information
Symbol
Dmel\kek1UAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Ghiglione
FlyBase ID
FBal0096870
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-kek1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The complete kek1 coding region is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of kek1Scer\UAS.cGa under the control of Scer\GAL4CY2 results in eggs with strongly ventralised chorions. Expression of kek1Scer\UAS.cGa under the control of Scer\GAL4GMR.PF results in a rough eye phenotype.

kek1Scer\UAS.cGa; Scer\GAL4Bx-MS1096 flies have a general vein loss phenotype. In kek1Scer\UAS.cGa; Scer\GAL4en-e16E flies this vein loss phenotype is restricted to the posterior compartment of the wing. kek1Scer\UAS.cGa; Scer\GAL4GMR.PF flies have substantially reduced numbers of photoreceptors and eye size.

When expression is driven by Scer\GAL4GMR.PF the eyes are rough and small.

Dorsal migration of border cells is severely inhibited when kek1Scer\UAS.cGa is expressed under the control of either Scer\GAL4slbo.2.6, with only minor effects on the initial posterior migration. Most eggs derived from these females do not hatch.

Eggs derived from females expressing kek1Scer\UAS.cGa under the control of Scer\GAL4T155 are longer than wild-type. They show a reduction or complete loss of dorsal appendages, due to a ventralisation of the eggshell.

Overexpression of kek1Scer\UAS.cGa driven by Scer\GAL471B results in loss of veins in the adult wing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4CY2, kek1UAS.cGa has egg chorion phenotype, suppressible | partially by Egfr[+]/EgfrSOK5

Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of kek1Scer\UAS.cGa in the follicle cells completely suppresses the dorsalised chorion phenotype caused by expression of grkΔTC.Scer\UAS under the control of Scer\GAL4CY2. The dorsalised chorion phenotype caused by expression of Egfr::kek1KEΔCG.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4CY2 is completely suppressed by coexpression of kek1Scer\UAS.cGa. The rough eye phenotype caused by expression of kek1Scer\UAS.cGa under the control of Scer\GAL4GMR.PF is suppressed if they are heterozygous for EgfrSOK1/+, EgfrSOK2/+, EgfrSOK3/+, EgfrSOK4/+ or EgfrSOK5/+. The ventralised chorion phenotype caused by expression of kek1Scer\UAS.cGa under the control of Scer\GAL4CY2 is suppressed by EgfrSOK3/+ or EgfrSOK4/+ and to a lesser extent by EgfrSOK5/+.

The ventralised phenotype of eggs derived from females expressing kek1Scer\UAS.cGa under the control of Scer\GAL4T155 is not altered if the females are also coexpressing rhoScer\UAS.cGa. The ventralised phenotype of eggs derived from females expressing kek1Scer\UAS.cGa under the control of Scer\GAL4T155 is overridden if the females are also coexpressing btl::EgfrScer\UAS.T:λ\cI-DD or phlScer\UAS.F179; the resulting eggs are dorsalised.

The addition of vnScer\UAS.cSa to flies expressing kek1Scer\UAS.cGa driven by Scer\GAL471B results in an additive phenotype, with both vein loss and ectopic vein material seen.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
kek1Scer\UAS.cGa
kek1UAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Ghiglione
Secondary FlyBase IDs
    References (6)