FlyBase Allele Report: Dmel\ebi[1-334.GMR]

General Information

Symbol

Dmel\ebi^1-334.GMR

Species

D. melanogaster

Name

FlyBase ID

FBal0096974

Feature type

allele

Associated gene

Dmel\ebi

Associated Insertion(s)

Carried in Construct

P{GMR-ebi.1-334}

Also Known As

GMR-ebi^DN

Key Links

Transgenic product class

dominant_negative_variant

(Tsuda et al., 2002)

inframe_deletion

(Dong et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Tsuda et al., 2002)

inframe_deletion

(Dong et al., 1999)

Mutagen

in vitro construct

(Dong et al., 1999)

Progenitor genotype

Carried in construct

P{GMR-ebi.1-334}

Cytology

Description

GMR regulatory sequences drive expression of a dominant negative form of ebi.

(Tsuda et al., 2002)

The expression of the first 334 amino acids of ebi is governed by GMR regulatory sequences.

(Dong et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

ebi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of sensory system disease

CEA

(Lim et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | progressive

(Lim et al., 2012)

Phenotype Manifest In

eye

(Lim et al., 2012, Tsuda et al., 2006, Voas and Rebay, 2003, Tsuda et al., 2002)

mitochondrion | progressive

(Lim et al., 2012)

ommatidium

(Voas and Rebay, 2003)

retina | progressive

(Lim et al., 2012)

Detailed Description

Statement

Reference

After eclosion, ebi^1-334.GMR/+ flies possess a near-normal complement of photoreceptors in each ommatidium. Five weeks later, however, these retinas are severely degenerated.

One copy of P{GMR-ebi.1-334} produces a slightly abnormal rough-eye phenotype, and two copies produces a severe rough-eye phenotype.

5 week old ebi^1-334.GMR/+ photoreceptor cells in the eye show defective mitochondria with swollen and abnormal cristae.

(Lim et al., 2012)

Expression of ebi^1-334.GMR causes a mild defect in eye morphology.

(Tsuda et al., 2006)

ebi^1-334.GMR homozygotes have a mild rough eye phenotype.

(Voas and Rebay, 2003)

ebi^1-334.GMR causes a weak eye phenotype.

(Tsuda et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ebi^1-334.GMR has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Diap1[+]/Diap1⁴

(Lim et al., 2012)

Suppressed by

Statement

Reference

ebi^1-334.GMR has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Df(3L)H99/+

(Lim et al., 2012)

ebi^1-334.GMR has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Jra¹/Jra[+]

(Lim et al., 2012)

ebi^1-334.GMR has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by hep[+]/hep^r75

(Lim et al., 2012)

ebi^1-334.GMR has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by BacA\p35^UAS.cHa/Scer\GAL4^GMR.PU

(Lim et al., 2012)

NOT suppressed by

Statement

Reference

ebi^1-334.GMR has abnormal neuroanatomy | adult stage | progressive phenotype, non-suppressible by hid⁰⁵⁰¹⁴/hid[+]

(Lim et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

ebi^1-334.GMR has retina | progressive phenotype, enhanceable by Diap1[+]/Diap1⁴

(Lim et al., 2012)

ebi^1-334.GMR has eye phenotype, enhanceable by Delta^9P/Dl[+]

(Tsuda et al., 2002)

Suppressed by

Statement

Reference

ebi^1-334.GMR has eye phenotype, suppressible by +/Df(2R)X1

(Lim et al., 2012)

ebi^1-334.GMR has eye phenotype, suppressible by Jra¹/Jra[+]

(Lim et al., 2012)

ebi^1-334.GMR has retina | progressive phenotype, suppressible by Df(3L)H99/+

(Lim et al., 2012)

ebi^1-334.GMR has retina | progressive phenotype, suppressible by Jra¹/Jra[+]

(Lim et al., 2012)

ebi^1-334.GMR has retina | progressive phenotype, suppressible by hep[+]/hep^r75

(Lim et al., 2012)

ebi^1-334.GMR has retina | progressive phenotype, suppressible by BacA\p35^UAS.cHa/Scer\GAL4^GMR.PU

(Lim et al., 2012)

ebi^1-334.GMR has ommatidium phenotype, suppressible by rno⁵/rno[+]

(Voas and Rebay, 2003)

ebi^1-334.GMR has eye phenotype, suppressible by rno²/rno[+]

(Voas and Rebay, 2003)

NOT suppressed by

Statement

Reference

ebi^1-334.GMR has retina | progressive phenotype, non-suppressible by hid⁰⁵⁰¹⁴/hid[+]

(Lim et al., 2012)

Enhancer of

Statement

Reference

ebi^1-334.GMR is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, chn^GS11450

(Tsuda et al., 2006)

ebi^1-334.GMR is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, chn^GS17605

(Tsuda et al., 2006)

NOT Enhancer of

Statement

Reference

ebi^1-334.GMR is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, chn^UAS.cTa

(Tsuda et al., 2006)

ebi^1-334.GMR is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, chn^GS2112

(Tsuda et al., 2006)

ebi^1-334.GMR is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, chn^GS17892

(Tsuda et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2R)X1/+ or Jra¹/+ suppresses the rough eye phenotype seen in flies with two copies of ebi^1-334.GMR.

Df(3L)H99/+, Jra¹/+, hep^r75/+, but not hid⁰⁵⁰¹⁴/+, suppresses the late-onset retinal degeneration phenotype of ebi^1-334.GMR/+ flies. Diap1⁴/+ enhances this phenotype.

(Lim et al., 2012)

Expression of ebi^1-334.GMR strongly enhances the eye morphology phenotype of Scer\GAL4^GMR.PF>chn^GS17605 and Scer\GAL4^GMR.PF>chn^GS11450 flies but fails to enhance the eye phenotype of Scer\GAL4^GMR.PF>chn^GS17892, Scer\GAL4^GMR.PF>chn^Scer\UAS.cTa and Scer\GAL4^GMR.PF>chn^GS2112 flies.

(Tsuda et al., 2006)

The mild rough eye phenotype of ebi^1-334.GMR homozygotes is almost completely suppressed by rno²/+ or rno⁵/+.

(Voas and Rebay, 2003)

The combination of ebi^1-334.GMR and Dl^9P/+ leads to flies with a significantly rough eye phenotype.

(Tsuda et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ebi^1-334.GMR is rescued by ebi^UAS.cTa/Scer\GAL4^GMR.PU

(Lim et al., 2012)

Comments

Scer\GAL4^GMR.PU-mediated expression of ebi^Scer\UAS.cTa rescues the retinal degeneration phenotype seen in 5-week old ebi^1-334.GMR/+ flies.

(Lim et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ebi^1-334.GMR

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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