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General Information
Symbol
Dmel\bonsaik08322
Species
D. melanogaster
Name
FlyBase ID
FBal0097167
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
bonsai1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{lacW} insertion in the 5' untranslated region.

Insertion 1bp 5' of bonsai transcription unit (which is 110bp 5' of the Cdk9 transcription unit).

Insertion components
P{lacW}bonsaik08322
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

anterior larval midgut & mitochondrion

Detailed Description
Statement
Reference

Mutant larvae show an extreme delay in growth and die before attaining wild-type size. At 25oC, the L1 stage lasts 2 days (instead of 1 day in wild-type animals), the L2 stage lasts at least 6 days (instead of 1 day in wild-type animals) and the L3 stage lasts at least 7 days (instead of 2 days in wild-type animals). Small adults occasionally eclose about 10 days later than wild-type flies, but they do not survive. Mutant larvae appear to have numerous mitochondria in the anterior midgut cells, but these mitochondria appear to be respiration-defective (as assayed by dye labelling). Gut pH is normal in mutant larvae. The mutant larvae are not deficient in the digestion of yeast cells and can absorb nutrients through their gut.

Larval growth reduced. 100% survive until 8 days after hatching. 50% survive until 11 days after hatching. 8% survive until 13 days after hatching. DNA replication continues. Mutant phenotype is not cell autonomous. Flies that escape lethality are small. In mutant larval brains, cell proliferation is impaired as compared to other tissues and compared to wild type. Mutant clones induced at 24, 36 and 42 hours after hatching grew to a size similar to wild type. No defects are observed in mosaic adults. Mutant clones in the eye survive well, suggesting non-autonomy of mutant phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

bonsaik08322 has phenotype, suppressible by Dphs.PD

bonsaik08322 has phenotype, suppressible by E2f1hs.PD

Additional Comments
Genetic Interactions
Statement
Reference

bonsaik08322 ft8 double mutant animals first exhibit the extreme growth delay seen in bonsaik08322 single mutants, reaching the L3 stage after 6 days instead of the normal 2 days when raised at 25oC. However, in mid-L3, double mutants discs start to grow and quickly show hyperplasia, as is seen in ft8 single mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Not rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Excision of the P-element is accompanied by reversion of the mutant phenotype.

Excision of the P{lacW} element can revert the mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)