FlyBase Allele Report: Mmus\Cd8a[UAS.cLa.EGFP]

General Information

Symbol

Mmus\Cd8a^UAS.cLa.EGFP

Species

M. musculus

Name

FlyBase ID

FBal0097215

Feature type

allele

Associated gene

Mmus\Cd8a

Associated Insertion(s)

Carried in Construct

P{UAS-mCD8::GFP.L}

Also Known As

UAS-mCD8-GFP, UAS-mCD8::GFP, UAS-CD8-GFP, UAS-mCD8GFP, UAS-CD8GFP, UAS-CD8::GFP, mCD8-GFP, mCD8::GFP, CD8-GFP, UAS-mCD8:GFP

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Lee and Luo, 1999)

Progenitor genotype

Carried in construct

P{UAS-mCD8::GFP.L}

Cytology

Description

Coding region for Mmus\Cd8a is fused in frame, at its 3' end to EGFP, and expression of the fusion protein is governed by UASt regulatory sequences.

(Lee and Luo, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Cd8a

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} in photoreceptors under the control of Scer\GAL4^GMR.PF produces electroretinogram depolarisation amplitudes that are similar to controls.

(Scappini et al., 2007)

Expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^CY2 in the follicle cells does not result in a mutant chorion phenotype.

(Alvarado et al., 2004)

Expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^C96 does not result in any wing defects. Expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF does not have any effect on the eye. Animals expressing Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^pnr-MD237 show wild type bristle formation.

(Presente et al., 2002)

Causes no toxicity throughout a cell's life.

(Lee and Luo, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF is a non-enhancer of abnormal neurophysiology phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Scappini et al., 2007)

Suppressor of

Statement

Reference

Scer\GAL4^e33C/Mmus\Cd8a^UAS.cLa.EGFP is a suppressor of abnormal developmental rate phenotype of asrij⁹

(Sinha et al., 2013)

NOT Suppressor of

Statement

Reference

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^P0.5.Pdf is a non-suppressor of abnormal circadian rhythm phenotype of Gαs^Q215L.UAS, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF is a non-enhancer of photoreceptor phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Scappini et al., 2007)

Suppressor of

Statement

Reference

Scer\GAL4^e33C/Mmus\Cd8a^UAS.cLa.EGFP is a suppressor of embryonic/larval plasmatocyte phenotype of asrij⁹

(Sinha et al., 2013)

NOT Suppressor of

Statement

Reference

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^slbo.2.6 is a non-suppressor of border follicle cell phenotype of Pvr^DN.UASp, Scer\GAL4^slbo.2.6

(Geisbrecht and Montell, 2004)

Mmus\Cd8a^UAS.cLa.EGFP/Scer\GAL4^slbo.2.6 is a non-suppressor of border follicle cell phenotype of Rac1^N17.UAS

(Geisbrecht and Montell, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Transgenic flies that express Scer\GAL4^e33C>Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} in asrij⁹ mutants show complete suppression of premature plasmatocyte differentiation.

(Sinha et al., 2013)

The arrhythmicity caused by expression of G-sα60A^{Q215L.Scer\UAS} in LN[[v]] neurons under the control of Scer\GAL4^P0.5.Pdf in constant darkness is not suppressed by co-expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}.

(Dahdal et al., 2010)

Expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} with Hsap\CHMP2B^{Intron5.Scer\UAS} does not affect the severity of the rough eye phenotype (both under the control of Scer\GAL4^GMR.PF).

(Ahmad et al., 2009)

Co-expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} does not enhance the reduction in electroretinogram depolarisation amplitude seen when Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} is expressed in photoreceptors under the control of Scer\GAL4^GMR.PF.

(Scappini et al., 2007)

The border follicle cell migration defect seen in egg chambers expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is not suppressed by coexpression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}. The border follicle cell migration defect seen in egg chambers expressing Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6 is not suppressed by coexpression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}.

(Geisbrecht and Montell, 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (112)

Bloomington

29715

P{UAS-salm.K}X, w^*; P{UAS-mCD8::GFP.L}LL5

7475

w^*; P{eve-GAL4.RN2}P, P{UAS-mCD8::GFP.L}LL5