FlyBase Allele Report: Dmel\GluRIIA[M614R.UAS.Tag:MYC]

General Information

Symbol

Dmel\GluRIIA^{M614R.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0097264

Feature type

allele

Associated gene

Dmel\GluRIIA

Associated Insertion(s)

Carried in Construct

P{UAS-GluRIIA.M614R}

Also Known As

UAS-GluRIIA^M/R, UAS-GluRIIA^M614R

Key Links

Transgenic product class

dominant_negative_variant

(DiAntonio et al., 1999)

missense_variant

(DiAntonio et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(DiAntonio et al., 1999)

missense_variant

(DiAntonio et al., 1999)

Mutagen

in vitro construct

(DiAntonio et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-GluRIIA.M614R}

Cytology

Description

UASt regulatory sequences drive expression of a dominant negative form of GluRIIA (carries the amino acid replacement M614R). The protein is tagged with Tag:MYC.

(DiAntonio et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

GluRIIA

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^C142

(Haghighi et al., 2003)

abnormal neurophysiology | larval stage, with Scer\GAL4^unspecified

(Penney et al., 2012)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^Mhc.PW

(Penney et al., 2016)

lethal, with Scer\GAL4^how-24B

(DiAntonio et al., 1999)

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^unspecified

(Penney et al., 2012)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Mhc.PW

(Penney et al., 2016)

Detailed Description

Statement

Reference

Expression of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Mhc.PW in larval neuromuscular junctions results in decreased amplitude of miniature excitatory junction currents (mEJCs) as compared to controls, EJCs are maintained at wild type levels, and quantal content is increased.

(Penney et al., 2016)

In neuromuscular junctions of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae there is a decrease in quantal size, but an increase in quantal content. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae).

(Haghighi et al., 2003)

Expression of two copies of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^how-24B results in lethality. Expression of one copy of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^how-24B results in viable adults which do not have any obvious behavioural abnormalities. There is a significant reduction in the quantal size at the neuromuscular junction.

(DiAntonio et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

GluRIIA^{M614R.UAS.Tag:MYC}, Scer\GAL4^unspecified has abnormal neurophysiology | larval stage phenotype, non-enhanceable by eIF4A[+]/eIF4A⁰²⁴³⁹

(Penney et al., 2012)

Suppressed by

Statement

Reference

GluRIIA^{M614R.UAS.Tag:MYC}, Scer\GAL4^C142 has abnormal neurophysiology phenotype, suppressible by wit^HA4/wit^HA5

(Haghighi et al., 2003)

GluRIIA^{M614R.UAS.Tag:MYC}, Scer\GAL4^C142 has abnormal neurophysiology phenotype, suppressible | partially by CaMKII^T287D.UAS

(Haghighi et al., 2003)

NOT suppressed by

Statement

Reference

GluRIIA^{M614R.UAS.Tag:MYC}, Scer\GAL4^unspecified has abnormal neurophysiology | larval stage phenotype, non-suppressible by eIF4A[+]/eIF4A⁰²⁴³⁹

(Penney et al., 2012)

Other

Statement

Reference

GluRIIA^{M614R.UAS.Tag:MYC}, Lrrk[+]/Lrrk^e03680, Scer\GAL4^Mhc.PW has abnormal neurophysiology | third instar larval stage phenotype

(Penney et al., 2016)

GluRIIA^{M614R.UAS.Tag:MYC}, Scer\GAL4^C142, wit^HA4/wit^HA5 has abnormal neurophysiology phenotype

(Haghighi et al., 2003)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

GluRIIA^{M614R.UAS.Tag:MYC}, Scer\GAL4^unspecified has embryonic/larval neuromuscular junction | larval stage phenotype, non-enhanceable by eIF4A[+]/eIF4A⁰²⁴³⁹

(Penney et al., 2012)

NOT suppressed by

Statement

Reference

GluRIIA^{M614R.UAS.Tag:MYC}, Scer\GAL4^unspecified has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by eIF4A[+]/eIF4A⁰²⁴³⁹

(Penney et al., 2012)

Enhancer of

Statement

Reference

Scer\GAL4^unspecified/GluRIIA^{M614R.UAS.Tag:MYC} is an enhancer of synaptic vesicle phenotype of hiw^unspecified

(DiAntonio et al., 2001)

Other

Statement

Reference

GluRIIA^{M614R.UAS.Tag:MYC}, Lrrk[+]/Lrrk^e03680, Scer\GAL4^Mhc.PW has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Penney et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Lrrk^e03680/+ blocks the increased quantal content seen in larval NMJs expressing GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Mhc.PW, via a decrease in excitatory junction current (EJC) amplitude.

(Penney et al., 2016)

The increase in quantal content in neuromuscular junctions of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae is partially suppressed by CaMKII^{T287D.Scer\UAS} : There is an decrease of 25% (p < 0.01) (55% decrease when corrected for nonlinear summation) in quantal content compared to GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae. This decrease is due to a significant reduction (p < 0.01) in the amplitude of excitatory postsynaptic potentials (EPSPs). There is no effect on mini-EPSPs. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). wit^HA4/wit^HA5 suppresses the increase in quantal content seen in the neuromuscular junctions of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). wit^HA4/wit^HA5 suppresses the increase in the excitatory postsynaptic potential amplitude seen in the neuromuscular junctions of Rat\CaMKIINα^{I.Scer\UAS.cHa}; Scer\GAL4^C142 larvae. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). The excitatory postsynaptic potential (EPSP) and mini-EPSP amplitudes are significantly decreased in wit^HA4/wit^HA5 larvae by the presence of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} with Scer\GAL4^C142. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae).

(Haghighi et al., 2003)

The addition off GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} to hiw mutants leads to a an 18% decrease in quantal size, no change in the size of the evoked response, and a compensatory 30% increase in quantal content.

(DiAntonio et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64256

w^*; P{UAS-GluRIIA.M614R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Glu-RIIA^{M614R.Scer\UAS.T:Hsap\MYC}

GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}

GluRIIA^{M614R.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close