Allele Dmel\GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0097264
Feature type	allele	Associated gene	Dmel\GluRIIA

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - amino acid replacement, in vitro construct - site directed mutagenesis
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (DiAntonio et al., 1999) in vitro construct - coding region fusion (DiAntonio et al., 1999) in vitro construct - regulatory fusion (DiAntonio et al., 1999) in vitro construct - site directed mutagenesis (DiAntonio et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: M614R. Mutant GluRIIA tagged with T:Hsap\MYC, is expressed under the control of Scer\UAS regulatory sequences. Encodes a dominant negative form of GluRIIA. (DiAntonio et al., 1999)
Carried in construct	P{UAS-GluRIIA.M614R} (Haghighi et al., 2003, DiAntonio et al., 1999, DiAntonio et al., 2001)
Tagged with	T:Hsap\MYC (DiAntonio et al., 1999, DiAntonio et al., 2001, Haghighi et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^how-24B (DiAntonio et al., 1999) neurophysiology defective, with Scer\GAL4^C142 (Haghighi et al., 2003)
Phenotype Manifest In

Detailed Description
	Statement Reference In neuromuscular junctions of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae there is a decrease in quantal size, but an increase in quantal content. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). (Haghighi et al., 2003) Expression of two copies of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^how-24B results in lethality. Expression of one copy of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^how-24B results in viable adults which do not have any obvious behavioural abnormalities. There is a significant reduction in the quantal size at the neuromuscular junction. (DiAntonio et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^C142 has neurophysiology defective phenotype, suppressible \| partially by CaMKII^{T287D.Scer\UAS} (Haghighi et al., 2003) GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^C142 has neurophysiology defective phenotype, suppressible by wit^HA4/wit^HA5 (Haghighi et al., 2003)
Other
Statement Reference GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^C142, wit^HA4/wit^HA5 has neurophysiology defective phenotype (Haghighi et al., 2003)
Phenotype Manifest In
Enhancer of
Statement Reference Scer\GAL4^unspecified/GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} is an enhancer of synaptic vesicle phenotype of hiw^unspecified (DiAntonio et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference The increase in quantal content in neuromuscular junctions of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae is partially suppressed by CaMKII^{T287D.Scer\UAS}: There is an decrease of 25% (p < 0.01) (55% decrease when corrected for nonlinear summation) in quantal content compared to GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae. This decrease is due to a significant reduction (p < 0.01) in the amplitude of excitatory postsynaptic potentials (EPSPs). There is no effect on mini-EPSPs. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). wit^HA4/wit^HA5 suppresses the increase in quantal content seen in the neuromuscular junctions of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^C142 larvae. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). wit^HA4/wit^HA5 suppresses the increase in the excitatory postsynaptic potential amplitude seen in the neuromuscular junctions of Rat\CaMKIINα^{I.Scer\UAS.cHa}; Scer\GAL4^C142 larvae. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). The excitatory postsynaptic potential (EPSP) and mini-EPSP amplitudes are significantly decreased in wit^HA4/wit^HA5 larvae by the presence of GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} with Scer\GAL4^C142. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). (Haghighi et al., 2003) The addition off GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} to hiw mutants leads to a an 18% decrease in quantal size, no change in the size of the evoked response, and a compensatory 30% increase in quantal content. (DiAntonio et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Glu-RIIA^{M614R.Scer\UAS.T:Hsap\MYC} GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Haghighi et al., 2003, Neuron 39(2): 255--267 Retrograde control of synaptic transmission by postsynaptic CaMKII at the Drosophila neuromuscular junction. [FBrf0160587] DiAntonio et al., 2001, Nature 412(6845): 449--452 Ubiquitination-dependent mechanisms regulate synaptic growth and function. [FBrf0137327] DiAntonio et al., 1999, J. Neurosci. 19(8): 3023--3032 Glutamate receptor expression regulates quantal size and quantal content at the Drosophila neuromuscular junction. [FBrf0107675]

Allele Dmel\GluRIIAM614R.Scer\UAS.T:Hsap\MYC

Allele Dmel\GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC}