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General Information
Symbol
Dmel\E2f1i2
Species
D. melanogaster
Name
FlyBase ID
FBal0097365
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dE2F1i2
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C21623523T

Reported nucleotide change:

C?T

Amino acid change:

Q527term | E2f1-PA; Q527term | E2f1-PB; Q527term | E2f1-PC; Q543term | E2f1-PD; Q543term | E2f1-PE; Q527term | E2f1-PF

Reported amino acid change:

Q527term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q527term.

Nucleotide substitution: C?T.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

E2fi2/E2f07172 trans-heterozygotes are viable and can complete oogenesis. Eggs laid by these females display a variety of dorsoventral polarity defects, including eggshells with fused dorsal appendages (ventralised, 22%), eggshells with fused but slightly expanded dorsal appendages (between dorsalised and ventralised, 39%), and eggshells with fused but widely spread dorsal appendages (strongly dorsalised, 11%). In addition, 8% of the eggs laid by E2fi2/E2f07172 females have greatly degenerated dorsal appendages, which obscure the dorsoventral polarity pattern of these eggshells.

In wild-type animals, exposure to 40Gy of γ rays induces a distinct pattern of apoptosis in the wing disc, focussed on the the wing pouch, but excluding the DV boundary region and prospective vein. In E2fi2 homozygotes, the same irradiation treatment induces less cell death was reduced in the intervein regions but increased cell death at the D/V boundary.

The ovaries of E2fi2/Df(3R)e-BS2 females are smaller than wild-type but have the normal complement of nurse and follicle cells. Occasionally, degenerating early egg chambers (stage 8 and earlier) are seen. The predominant defects in E2fi2/Df(3R)e-BS2 females are a failure of nurse cells to deposit their contents into the oocyte and degenerate and an increased amount of heterochromatic DNA in the nurse cell nuclei. This appears to be caused by continued replication, when normally replication is blocked by a reduction in E2f activity. In E2fi2/Df(3R)e-BS2 mutants defects in nurse cell chromosome morphology are also seen. Although mutant chromosomes go through the polytene and bulbous states, the homologous copies of each of the large chromosomes are frequently separated rather than associated as in wild-type. The fourth chromosome is always a single sphere. Sister chromatids often do not separate after the bulbous stage, becoming more condensed and having a polytene rather than interphase appearance. Individual chromosome arms are visible, and the chromosomes are clustered as if the homologous sister chromatids remain in proximity. In stage 12 E2fi1/Df(3R)e-BS2 egg chambers the nurse cell nuclear envelope does not break down as normal. In homozygous E2fi2 egg chambers, unlike wild-type (where nurse cells undergo apoptosis during stages 12 to 13 and have degenerated by stage 14), degeneration of nurse cells is abnormal and occurs late if at all. E2fi2/Df(3R)e-BS2 mutant mothers lay few eggs. In the rare cases in which mature eggs are laid most are unfertilized, apparently due to defects in chorion structure. The few fertilized embryos arrest in early division cycles. In addition, the morphology of the polar bodies are affected. In embryos collected from these mothers some have normal polar body rosettes whereas others show aberrant morphology. Only a few eggs show some nuclear divisions, but with aberrant chromosome condensation.

Hemizygotes are fully viable and have slightly rough eyes. Hemizygous females have reduced fertility. E2fi1/E2fi2 flies are fully viable and fertile. One copy of CycE05206 has no effect on the viability of E2fi2/E2f91 flies. E2fi2 flies produce thick and rough eggshells. The size and intensity of foci of BrdU incorporation are increased in the follicle cells of hemizygous egg chambers compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ;Stam19 double homozygous somatic clones in third instar larval eye disc is completely abolished when the clones are created in E2f1[i2]/E2f1[rM729] mutant background.

The presence of an E2fi2 background significantly decreases gig64/Rbf15aΔ induced cell death in the eye. In addition, much larger gig64/Rbf15aΔ double mutant clones are observed in adult eyes.

The presence of an E2fi2 background significantly decreases gig192/Rbf15aΔ induced cell death in the eye. In addition, much larger gig192/Rbf15aΔ double mutant clones are observed in adult eyes.

The shiny-eye and multiple photoreceptor-R8 phenotype seen in rno1, Rbf15aΔ eye somatic mutant clones is significantly suppressed in a E2fi2/E2frM729 mutant background.

Approximately 50% of the expected number of Rbf14/Y ; E2fi2/E2frM729 flies survive to the adult stage, and the development of these flies is not significantly delayed compared to sibling flies. The surviving flies have normal eyes, normal macrochaetae on the notum and normal wings and legs.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (14)