Allele Dmel\Axn^S044230

General Information
Symbol	Dmel\Axn^S044230	Species	D. melanogaster
Name		FlyBase ID	FBal0097414
Feature type	allele	Associated gene	Dmel\Axn
Also Known As	axin^S044230

Allele class
Mutagen	Delta2-3, P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Deak et al., 1997) P-element activity (Hamada et al., 1999, Szeged Stock Center, 1998-)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{lacW} insertion in the first exon, 135bp downstream of the transcription start site. (Hamada et al., 1999)
Caused by insertion	P{lacW}Axn^S044230 (Hamada et al., 1999, Szeged Stock Center, 1998-, Tolwinski et al., 2003, Tolwinski and Wieschaus, 2004, Giraldez and Cohen, 2003, Tomoyasu et al., 2000, Deak et al., 1997, Roberts et al., 2012, Kalamegham et al., 2007, Fiedler et al., 2011, Tolwinski, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective \| somatic clone \| third instar larval stage (Roberts et al., 2012) lethal \| larval stage \| recessive (Hamada et al., 1999) lethal \| recessive (Szeged Stock Center, 1998-, Deak et al., 1997) visible \| somatic clone (Ahmed et al., 2002, Hamada et al., 1999)
Phenotype Manifest In
	adult abdomen & macrochaeta \| somatic clone (Hamada et al., 1999) adult abdomen \| somatic clone (Hamada et al., 1999) denticle \| supernumerary, with Axn^Scer\UAS.cHa, Scer\GAL4^da.G32 (Tolwinski, 2009) denticle belt (Tolwinski et al., 2003, Hamada et al., 1999) denticle belt, with Axn^Scer\UAS.cHa, Scer\GAL4^da.G32 (Tolwinski, 2009) denticle belt, with Axn^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}, Scer\GAL4^da.G32 (Tolwinski, 2009) denticle belt \| germline clone (Tolwinski, 2009) embryonic/first instar larval cuticle (Tolwinski et al., 2003, Fiedler et al., 2011) follicle cell \| somatic clone (Song and Xie, 2003) follicle stem cell \| somatic clone (Song and Xie, 2003) G1 phase \| somatic clone (Giraldez and Cohen, 2003) germline cyst \| somatic clone (Song and Xie, 2003) intestinal stem cell \| somatic clone (Lee et al., 2009) leg \| ectopic (Hamada et al., 1999) leg \| somatic clone (Hamada et al., 1999) macrochaeta \| ectopic \| somatic clone (Hamada et al., 1999) wing \| somatic clone (Ahmed et al., 2002, Hamada et al., 1999) wing disc \| somatic clone \| third instar larval stage (Roberts et al., 2012) wing margin bristle \| cell autonomous \| ectopic \| somatic clone (Hamada et al., 1999) wing margin bristle \| ectopic \| somatic clone (Ahmed et al., 2002)
Detailed Description
	Statement Reference Axn[S044230] mutant clone cells in third instar wing discs apically constrict and invaginate to form cysts. (Roberts et al., 2012) Axn[S044230] mutant embryos display an entirely naked cuticle. (Fiedler et al., 2011) Homozygous intestinal stem cell clones in the adult midgut show hyperplasia. (Lee et al., 2009) Homozygous Axn[S044230] germ-line clone-derived embryos are completely devoid of ventral denticles. Homozygous Axn[S044230] germ-line clone-derived embryos expressing Axn[Scer\UAS.cHa] under the control of Scer\GAL4[da.G32] show supernumerary denticles in the ventral embryonic epidermis. Expression of Axn[Scer\UAS.T:Src64B,T:Zzzz\FLAG] under the control of Scer\GAL4[da.G32] can restore ventral denticle belt patterning to some extent of homozygous Axn[S044230] germ-line clone-derived embryos. (Tolwinski, 2009) BrdU incorporation is increased in Axn^S044230/Axn^S044230 somatic clones in the third instar wing disc. This effect is cell autonomous, and strongest when clones are located in the proximal regions of the wing pouch or in the prospective wing hinge and is not seen in clones at the center of the wing pouch. Flow cytometry analysis shows that these mutant clones contain a lower proportion of cells in G1 than in wild-type. (Giraldez and Cohen, 2003) Axn^S044230 somatic stem cell (SSC) clones in the ovary are lost more rapidly than wild-type clones. In germaria carrying both wild-type and Axn^S044230 mutant SSC clones, mutant follicle cells appear more in number than wild-type ones, indicating that Axn^S044230 SSCs and/or follicle cells are over-proliferative. In germaria where most or all the follicle cells are mutant for Axn^S044230, germline cysts do not undergo normal morphological changes from ball-like structures in region 2a to lens-shape cysts in region 2b, remaining in ball-like structures in region 2b. Mutant follicle cells do not integrate properly into egg chambers and accumulate instead as a disorganised mass between egg chambers. The mutant follicle cells have abnormal morphology, but neighbouring wild-type cells are normal in morphology. (Song and Xie, 2003) The cuticles produced by maternal/zygotic Axn^S044230/Axn^S044230 embryos consist entirely of smooth cuticle, with the exception of the T1 beard. (Tolwinski et al., 2003) Homozygous clones in the wing show transformations of wing blade to wing margin tissue. (Ahmed et al., 2002) Homozygotes die as first instar larvae. Homozygous embryos (lacking zygotic Axn function) have almost normal segment patterning. Embryos lacking both maternal and zygotic Axn function are completely naked, lacking all denticles. Embryos lacking maternal Axn function and having one paternal wild-type copy of Axn have some denticles on the ventral cuticle. Homozygous clones in the wing cell autonomously produce ectopic margin bristles. Homozygous clones in the leg produce a supernumerary leg from the dorsal side of the normal leg. Ectopic bristles are formed in the pleura by homozygous clones in the abdomen. (Hamada et al., 1999) Early lethal. (Deak et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Axn[+]/Axn^S044230, Scer\GAL4^vg.PU is a suppressor of visible phenotype of Scer\GAL4^vg.PU, gskt^Scer\UAS.cKa (Kalamegham et al., 2007)
Other
Statement Reference Apc2^19-3, Apc^Q8, Axn^S044230 has neuroanatomy defective \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012) Apc2³³, Apc^Q8, Axn^S044230 has neuroanatomy defective \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Apc2^d40, Apc^Q8, Axn^S044230 has denticle belt \| germline clone phenotype, non-enhanceable by Apc2^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^da.G32 (Tolwinski, 2009) Apc2^d40, Apc^Q8, Axn^S044230 has denticle belt \| germline clone phenotype, non-enhanceable by Apc2^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}/Scer\GAL4^da.G32 (Tolwinski, 2009) Apc2^d40, Apc^Q8, Axn^S044230 has denticle belt \| germline clone phenotype, non-enhanceable by Axn^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 has denticle belt \| germline clone phenotype, non-enhanceable by Apc2^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 has denticle belt \| germline clone phenotype, non-enhanceable by Apc2^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 has denticle belt \| germline clone phenotype, non-enhanceable by Apc^Q8/Apc2^d40 (Tolwinski, 2009) Axn^S044230 has embryonic/first instar larval cuticle phenotype, non-enhanceable by wg^l-17 (Tolwinski et al., 2003)
Suppressed by
Statement Reference Apc2^d40, Apc^Q8, Axn^S044230 has denticle belt \| germline clone phenotype, suppressible by Axn^Scer\UAS.cHa/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 has intestinal stem cell \| somatic clone phenotype, suppressible by pan^ΔN.Scer\UAS/Scer\GAL4^tub.PU (Lee et al., 2009)
NOT suppressed by
Statement Reference Apc2^d40, Apc^Q8, Axn^S044230 has denticle belt \| germline clone phenotype, non-suppressible by Apc2^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^da.G32 (Tolwinski, 2009) Apc2^d40, Apc^Q8, Axn^S044230 has denticle belt \| germline clone phenotype, non-suppressible by Apc2^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}/Scer\GAL4^da.G32 (Tolwinski, 2009) Apc2^d40, Apc^Q8, Axn^S044230 has denticle belt \| germline clone phenotype, non-suppressible by Axn^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 has denticle belt \| germline clone phenotype, non-suppressible by Apc2^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 has denticle belt \| germline clone phenotype, non-suppressible by Apc2^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 has denticle belt \| germline clone phenotype, non-suppressible by Apc^Q8/Apc2^d40 (Tolwinski, 2009) Axn^S044230 has denticle belt phenotype, non-suppressible by wg^l-17 (Tolwinski et al., 2003)
Suppressor of
Statement Reference Axn[+]/Axn^S044230, Scer\GAL4^vg.PU is a suppressor of wing margin phenotype of Scer\GAL4^vg.PU, gskt^Scer\UAS.cKa (Kalamegham et al., 2007) Axn^S044230 is a suppressor \| maternal effect \| partially of denticle belt \| ectopic phenotype of wg^l-17 (Tolwinski et al., 2003) Axn^S044230 is a suppressor \| maternal effect \| partially of embryonic/first instar larval cuticle phenotype of wg^l-17 (Tolwinski et al., 2003)
Other
Statement Reference Apc2^19-3, Apc^Q8, Axn^S044230 has medulla \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012) Apc2³³, Apc^Q8, Axn^S044230 has medulla \| somatic clone \| third instar larval stage phenotype (Roberts et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Apc2[19-3] Apc[Q8] Axn[S044230] triple mutant clone cells generated in the medullar region of third instar larval brains sometimes segregate from their neighbours to form cysts. When clones are induced in the medullar neurons the axons extend correctly to the medullar neuropil. (Roberts et al., 2012) The hyperplasia seen in Axn[S044230] intestinal stem cell clones in the adult midgut is completely suppressed if they are also expressing pan[ΔN.Scer\UAS] under the control of Scer\GAL4[tub]. (Lee et al., 2009) Expression of Apc2[Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[da.G32] has no effect on the ventral denticle belt phenotype of the germ-line and zygotic Axn[S044230]-mutation. Expression of Apc2[Scer\UAS.T:Src64B,T:Zzzz\FLAG] under the control of Scer\GAL4[da.G32] has no effect on the ventral denticle belt phenotype of the germ-line and zygotic Axn[S044230]-mutants. Triple mutants for Axn[S044230], Apc[Q8] and Apc2[d40] have naked ventral embryonic epidermis. The ventral denticle belt phenotype of Axn[S044230], Apc[Q8], Apc2[d40] triple mutants is suppressed by expression of Axn[Scer\UAS.cHa] under the control of Scer\GAL4[da.G32]. Expression of Axn[Scer\UAS.T:Src64B,T:Zzzz\FLAG] under the control of Scer\GAL4[da.G32] has no effect on the ventral denticle belt phenotype of Axn[S044230], Apc[Q8], Apc2[d40] triple mutants. Expression of Apc2[Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[da.G32] has no effect on the ventral denticle belt phenotype of Axn[S044230], Apc[Q8], Apc2[d40] triple mutants. Expression of Apc2[Scer\UAS.T:Src64B,T:Zzzz\FLAG] under the control of Scer\GAL4[da.G32] has no effect on the ventral denticle belt phenotype of Axn[S044230], Apc[Q8], Apc2[d40] triple mutants. (Tolwinski, 2009) The Scer\GAL4[vg.PU]-driven gskt[Scer\UAS.cKa]-overexpression phenotype is suppressed by Axn[S044230]/+ in the wing. (Kalamegham et al., 2007) The loss of segmentation and lack of smooth cuticle due to wg^l-17/wg^l-17 is partially maternally suppressed by Axn^S044230/Axn^S044230. If these embryos are also zygotically Axn^S044230/Axn^S044230, they produce cuticles consisting entirely of smooth cuticle (apart from the T1 beard), just as they would if they were wild-type for wg. The denticle belts formed in sgg^M11; arm^F1α maternal/zygotic double mutant embryos are largely suppressed by Axn^S044230/Axn^S044230, leaving mainly naked cuticle without much sign of periodic character. (Tolwinski et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Axn^S044230 is rescued by Axn^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^arm.PS (Fiedler et al., 2011)
Partially rescued by	Axn^S044230 is partially rescued by Axn^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^Antp.PU (Fiedler et al., 2011) Axn^S044230 is partially rescued by Axn^{Scer\UAS.T:Myr-Src64B,T:Zzzz\FLAG}/Scer\GAL4^da.G32 (Tolwinski, 2009) Axn^S044230 is partially rescued by Scer\GAL4^Antp.PU/Axn^{M3.Scer\UAS.T:Avic\GFP} (Fiedler et al., 2011) Axn^S044230 is partially rescued by Scer\GAL4^arm.PS/Axn^{M2.Scer\UAS.T:Avic\GFP} (Fiedler et al., 2011) Axn^S044230 is partially rescued by Scer\GAL4^arm.PS/Axn^{M4.Scer\UAS.T:Avic\GFP} (Fiedler et al., 2011) Axn^S044230 is partially rescued by Scer\GAL4^unspecified/Axn^Scer\UAS.cHa (Hamada et al., 1999)
Not rescued by	Axn^S044230 is not rescued by Axn^Scer\UAS.cHa/Scer\GAL4^da.G32 (Tolwinski, 2009)
Comments	Expression of Axn[Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[arm.PS] fully restores complete and normal denticle belts in 80% of Axn[S044230] mutant embryos. Expression of Axn[M2.Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[arm.PS] fully restores complete and normal denticle belts in only 59% of Axn[S044230] mutant embryos. Expression of Axn[M4.Scer\UAS.T:Avic\GFP]under the control of Scer\GAL4[arm.PS] fully restores complete and normal denticle belts in only 36% of Axn[S044230] mutant embryos. Expression of Axn[M3.Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[Antp.PU] restores complete and normal denticle belts in only 10% of Axn[S044230] mutant embryos. Expression of Axn[Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[Antp.PU] rescues the denticle phenotype in 32% of Axn[S044230] mutant embryos. (Fiedler et al., 2011) Homozygous Axn[S044230] germ-line clone-derived embryos expressing Axn[Scer\UAS.cHa] under the control of Scer\GAL4[da.G32] show supernumerary denticles in the ventral embryonic epidermis. Expression of Axn[Scer\UAS.T:Src64B,T:Zzzz\FLAG] under the control of Scer\GAL4[da.G32] can restore ventral denticle belt patterning to some extent of homozygous Axn[S044230] germ-line clone-derived embryos. (Tolwinski, 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Precise excision of the P{lacW} element reverts the lethal phenotype. (Hamada et al., 1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	0442/30 (Deak et al., 1997) axin^S044230 (Tolwinski and Wieschaus, 2004) Axin^S044230 (Ahmed et al., 2002, Silver et al., 2007, Roberts et al., 2012) Axin^s044230 (Takacs et al., 2008, Takacs et al., 2008, Lee et al., 2009) axn^S044230 (Tolwinski and Wieschaus, 2001) Axn^S044230 dAxin^l<up>3SO44230</up> (Kalamegham et al., 2007) l(3)S044230 (Hamada et al., 1999) l(3)S044230^S044230
Name Synonym	axin^S044230 (Tolwinski, 2009)
Secondary FlyBase IDs
	FBal0100102
References ( 18 )
Research paper	Roberts et al., 2012, PLoS ONE 7(2): e31284 Defining Components of the ßcatenin Destruction Complex and Exploring Its Regulation and Mechanisms of Action during Development. [FBrf0217546] Fiedler et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(5): 1937--1942 Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating {beta}-catenin. [FBrf0212953] Lee et al., 2009, Development 136(13): 2255--2264 Adenomatous polyposis coli regulates Drosophila intestinal stem cell proliferation. [FBrf0208211] Tolwinski, 2009, Genetics 181(3): 1169--1173 Membrane bound axin is sufficient for Wingless signaling in Drosophila embryos. [FBrf0207525] Takacs et al., 2008, Science 319(5861): 333--336 Dual positive and negative regulation of wingless signaling by adenomatous polyposis coli. [FBrf0202792] Kalamegham et al., 2007, Mol. Biol. Evol. 24(3): 732--742 Drosophila mojoless, a retroposed GSK-3, has functionally diverged to acquire an essential role in male fertility. [FBrf0201863] Silver et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(46): 18151--18156 Functional screening identifies miR-315 as a potent activator of Wingless signaling. [FBrf0202770] Tolwinski and Wieschaus, 2004, PLoS Biol. 2(4): e95 A nuclear function for armadillo/β-catenin. [FBrf0179090] Giraldez and Cohen, 2003, Development 130(26): 6533--6543 Wingless and Notch signaling provide cell survival cues and control cell proliferation during wing development. [FBrf0167523] Song and Xie, 2003, Development 130(14): 3259--3268 wingless signaling regulates the maintenance of ovarian somatic stem cells in Drosophila. [FBrf0160968] Tolwinski et al., 2003, Dev. Cell 4(3): 407--418 Wg/Wnt signal can be transmitted through arrow/LRP5,6 and Axin independently of Zw3/Gsk3 activity. [FBrf0158859] Ahmed et al., 2002, Development 129(7): 1751--1762 Drosophila Apc1 and Apc2 regulate Wingless transduction throughout development. [FBrf0146980] Tolwinski and Wieschaus, 2001, Development 128(11): 2107--2117 Armadillo nuclear import is regulated by cytoplasmic anchor Axin and nuclear anchor dTCF/Pan. [FBrf0136754] Tomoyasu et al., 2000, Mech. Dev. 96(1): 37--49 The Decapentaplegic morphogen gradient regulates the notal wingless expression through induction of pannier and u-shaped in Drosophila. [FBrf0130117] Deak et al., 1997, Genetics 147(4): 1697--1722 P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: correlation of physical and cytogenetic maps in chromosomal region 86E-87F. [FBrf0099762]
Supplementary material	Takacs et al., 2008, Science 319(5861): Supporting online material. [FBrf0202572]
Review	Hamada et al., 1999, Science 283(5408): 1739--1742 Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin. [FBrf0107755]
Stock list	Szeged Stock Center, 1998-, Szeged Stock Center P-element collection. (Computer file) Szeged Stock Center P-element collection. (Computer file) [FBrf0111308]

Allele Dmel\AxnS044230

Allele Dmel\Axn^S044230