adult abdomen & macrochaeta | somatic clone
Follicle cell clones both homozygous for AxnS044230 and expressing cosKK106270 under the control of Scer\GAL4Act.PU exhibit a more severe supernumerary stalk cell phenotype than either single mutant clones. The supernumerary polar/stalk cell phenotype induced by AxnS044230 homozygous follicle cell clones is enhanced by the additional clonal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act.PU.
Adulthood-generated Tnks19, AxnS044230 double homozygous somatic clones expressing the AxnT:SV5\V5 rescue transgene present a significant decrease in cell number as compared to adulthood-generated Tnks19 single homozygous somatic clones.
AxnS044230 enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones. Apoptosis is seen both anterior and posterior to the morphogenetic furrow in the double mutants. Inhibiting wg signalling through expression of panΔN.Scer\UAS under the control of a GAL4 driver suppresses this enhancement.
Expression of raptorHMS00124 under the control of a GAL4 driver significantly decreases the increase in apoptosis anterior to the morphogenetic furrow seen in AxnS044230 Rbf15aΔ mutant eye disc clones.
Rbf15aΔ enhances the reduction in ATP/ADP ratio seen in eye discs with AxnS044230 mutant clones (induced in a minute background). This enhancement is suppressed to the levels seen in AxnS044230 single mutants by Rheb4L1.
Apc219-3 ApcQ8 AxnS044230 triple mutant clone cells generated in the medullar region of third instar larval brains sometimes segregate from their neighbours to form cysts. When clones are induced in the medullar neurons the axons extend correctly to the medullar neuropil.
The loss of segmentation and lack of smooth cuticle due to wgl-17/wgl-17 is partially maternally suppressed by AxnS044230/AxnS044230. If these embryos are also zygotically AxnS044230/AxnS044230, they produce cuticles consisting entirely of smooth cuticle (apart from the T1 beard), just as they would if they were wild-type for wg. The denticle belts formed in sggM11; armF1α maternal/zygotic double mutant embryos are largely suppressed by AxnS044230/AxnS044230, leaving mainly naked cuticle without much sign of periodic character.
Expression of AxnM3.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Antp.PU restores complete and normal denticle belts in only 10% of AxnS044230 mutant embryos. Expression of AxnScer\UAS.T:Avic\GFP under the control of Scer\GAL4Antp.PU rescues the denticle phenotype in 32% of AxnS044230 mutant embryos.
Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnH504L.αTub84B.T:Zzzz\FLAG survive to adulthood without defects. Subsequently, if the rescued animals are mated with each other, they produce a few larvae and misshapen pupae that subsequently die. This indicates that both sexes of the parents are fertile.
AxnL501P.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG fails to fully rescue AxnS044230 mutants to adult viability. The survival of some of the rescued animals is extended into the pupal stage.
Expression of AxnScer\UAS.T:Src64B,T:Zzzz\FLAG under the control of Scer\GAL4da.G32 can restore ventral denticle belt patterning to some extent of homozygous AxnS044230 germ-line clone-derived embryos.
Maternal expression of AxnΔRGS.αTub84B.T:Zzzz\FLAG in AxnS044230 mutant (maternal-/zygotic-) embryos almost completely restores the wild-type pattern of denticle belts, although a few row 1 denticles are frequently missing.
Maternal expression of AxnαTub84B.T:Zzzz\FLAG in AxnS044230 mutant (maternal-/zygotic-) embryos restores the wild-type pattern of denticle belts. No ectopic denticles are present. Maternal expression of AxnS044230 allows AxnS044230 (maternal-/zygotic-) embryos to hatch.
Heteroallelic combination of AxnΔRGS.αTub84B.T:Zzzz\FLAG and AxnΔArm.αTub84B.T:Zzzz\FLAG rescues AxnS044230 mutants (maternal-/zygotic-) to viability. Cuticle preparations of the rescued embryos reveal a wild-type pattern of denticle band segmentation with only the occasional loss of denticles in row 1. The rescued flies exhibit normal wing formation without any abnormal loss or gain of margin bristles.