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General Information
Symbol
Dmel\AxnS044230
Species
D. melanogaster
Name
FlyBase ID
FBal0097414
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Axins044230, axinnull
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{lacW} insertion in the first exon, 135bp downstream of the transcription start site.

Insertion components
P{lacW}AxnS044230
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

adult abdomen & macrochaeta | somatic clone

Detailed Description
Statement
Reference

AxnS044230/Axn1511 transheterozygotes are lethal.

AxnS044230 homozygous follicle cell clones lead to a small increase in the numbers of polar/stalk cells, as compared to controls; early stage clones (in region 3 germarium or stage 2 egg chambers) do not show any significant changes in apoptosis (i.e. cDcp1-positive cells) or in replicating (i.e. Edu-positive) cells, as compared to controls. Mosaic border cell/polar cell clusters containing AxnS044230 clones present increased total cell number, as compared to controls.

The ATP/ADP ratio of eye discs with AxnS044230 mutant clones (induced in a minute background) is slightly lower than in controls.

AxnS044230 mutant clone cells in third instar wing discs apically constrict and invaginate to form cysts.

AxnS044230 mutant embryos display an entirely naked cuticle.

All AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnL447Q.αTub84B.T:Zzzz\FLAG die prior to hatching.

Nearly all of the AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnL447S.αTub84B.T:Zzzz\FLAG die prior to hatching.

Most of the AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG hatch successfully.

More than half of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnH504L.αTub84B.T:Zzzz\FLAG hatch successfully.

About half of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnL501P.αTub84B.T:Zzzz\FLAG hatch successfully.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnL501P.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnH504L.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnL447S.αTub84B.T:Zzzz\FLAG shows loss of denticles.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnL447Q.αTub84B.T:Zzzz\FLAG shows loss of denticles.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnH504L.αTub84B.T:Zzzz\FLAG survive to adulthood without defects. Subsequently, if the rescued animals are mated with each other, they produce a few larvae and misshapen pupae that subsequently die. This indicates that both sexes of the parents are fertile.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnL501P.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnK446E.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnL447S.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnL447Q.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG can rescue AxnS044230 mutants to adult viability and normal morphology.

AxnL501P.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG fails to fully rescue AxnS044230 mutants to adult viability. The survival of some of the rescued animals is extended into the pupal stage.

AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnK446E.αTub84B.T:Zzzz\FLAG can fully rescue AxnS044230.

Homozygous intestinal stem cell clones in the adult midgut show hyperplasia.

Homozygous AxnS044230 germ-line clone-derived embryos are completely devoid of ventral denticles.

Homozygous AxnS044230 germ-line clone-derived embryos expressing AxnScer\UAS.cHa under the control of Scer\GAL4da.G32 show supernumerary denticles in the ventral embryonic epidermis.

Expression of AxnScer\UAS.T:Src64B,T:Zzzz\FLAG under the control of Scer\GAL4da.G32 can restore ventral denticle belt patterning to some extent of homozygous AxnS044230 germ-line clone-derived embryos.

AxnS044230 mutant (maternal-/zygotic-) embryos lack all denticles.

AxnS044230 (maternal-/zygotic-) is lethal. The AxnS044230 mutant embryos fail to hatch.

Heteroallelic combination of AxnΔRGS.αTub84B.T:Zzzz\FLAG and AxnΔArm.αTub84B.T:Zzzz\FLAG rescues AxnS044230 mutants (maternal-/zygotic-) to viability. Cuticle preparations of the rescued embryos reveal a wild-type pattern of denticle band segmentation with only the occasional loss of denticles in row 1. The rescued flies exhibit normal wing formation without any abnormal loss or gain of margin bristles.

The majority of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔI.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

Maternal expression of AxnΔRGS.αTub84B.T:Zzzz\FLAG in AxnS044230 (maternal-/zygotic-) embryos almost completely restores the wild-type pattern of denticle belts, although a few row 1 denticles are frequently missing.

The majority of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔRGS.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔSgg.αTub84B.T:Zzzz\FLAG shows a marked loss of denticles.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔArm.αTub84B.T:Zzzz\FLAG shows a loss of denticles, including a frequent loss of row 1 denticles.

About half of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔArm.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔPP2A.αTub84B.T:Zzzz\FLAG exhibit deletions of smooth cuticle bands and the appearance of ectopic denticles.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔDIX.αTub84B.T:Zzzz\FLAG exhibit deletions of smooth cuticle bands and the appearance of ectopic denticles.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔPP2A.DIX.αTub84B.T:Zzzz\FLAG show the loss of smooth cuticle.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔRGS.Arm.αTub84B.T:Zzzz\FLAG lack all denticles.

BrdU incorporation is increased in AxnS044230/AxnS044230 somatic clones in the third instar wing disc. This effect is cell autonomous, and strongest when clones are located in the proximal regions of the wing pouch or in the prospective wing hinge and is not seen in clones at the center of the wing pouch. Flow cytometry analysis shows that these mutant clones contain a lower proportion of cells in G1 than in wild-type.

AxnS044230 somatic stem cell (SSC) clones in the ovary are lost more rapidly than wild-type clones. In germaria carrying both wild-type and AxnS044230 mutant SSC clones, mutant follicle cells appear more in number than wild-type ones, indicating that AxnS044230 SSCs and/or follicle cells are over-proliferative. In germaria where most or all the follicle cells are mutant for AxnS044230, germline cysts do not undergo normal morphological changes from ball-like structures in region 2a to lens-shape cysts in region 2b, remaining in ball-like structures in region 2b. Mutant follicle cells do not integrate properly into egg chambers and accumulate instead as a disorganised mass between egg chambers. The mutant follicle cells have abnormal morphology, but neighbouring wild-type cells are normal in morphology.

The cuticles produced by maternal/zygotic AxnS044230/AxnS044230 embryos consist entirely of smooth cuticle, with the exception of the T1 beard.

Homozygous clones in the wing show transformations of wing blade to wing margin tissue.

Homozygotes die as first instar larvae. Homozygous embryos (lacking zygotic Axn function) have almost normal segment patterning. Embryos lacking both maternal and zygotic Axn function are completely naked, lacking all denticles. Embryos lacking maternal Axn function and having one paternal wild-type copy of Axn have some denticles on the ventral cuticle. Homozygous clones in the wing cell autonomously produce ectopic margin bristles. Homozygous clones in the leg produce a supernumerary leg from the dorsal side of the normal leg. Ectopic bristles are formed in the pleura by homozygous clones in the abdomen.

Early lethal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Follicle cell clones both homozygous for AxnS044230 and expressing cosKK106270 under the control of Scer\GAL4Act.PU exhibit a more severe supernumerary stalk cell phenotype than either single mutant clones. The supernumerary polar/stalk cell phenotype induced by AxnS044230 homozygous follicle cell clones is enhanced by the additional clonal expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4Act.PU.

The increase in the numbers of intestinal progenitor cells and mitotic cells present in Tnks19/Tnks503 transheterozygous mutant adult midguts is suppressed by AxnS044230 heterozygosity.

Adulthood-generated Tnks19, AxnS044230 double homozygous somatic clones expressing the AxnT:SV5\V5 rescue transgene present a significant decrease in cell number as compared to adulthood-generated Tnks19 single homozygous somatic clones.

AxnS044230 enhances the increase in apoptosis anterior to the morphogenetic furrow seen in Rbf15aΔ mutant eye disc clones. Apoptosis is seen both anterior and posterior to the morphogenetic furrow in the double mutants. Inhibiting wg signalling through expression of panΔN.Scer\UAS under the control of a GAL4 driver suppresses this enhancement.

Rheb4L1 significantly decreases the increase in apoptosis anterior to the morphogenetic furrow seen in AxnS044230 Rbf15aΔ mutant eye disc clones.

Expression of raptorHMS00124 under the control of a GAL4 driver significantly decreases the increase in apoptosis anterior to the morphogenetic furrow seen in AxnS044230 Rbf15aΔ mutant eye disc clones.

Rbf15aΔ enhances the reduction in ATP/ADP ratio seen in eye discs with AxnS044230 mutant clones (induced in a minute background). This enhancement is suppressed to the levels seen in AxnS044230 single mutants by Rheb4L1.

Apc219-3 ApcQ8 AxnS044230 triple mutant clone cells generated in the medullar region of third instar larval brains sometimes segregate from their neighbours to form cysts. When clones are induced in the medullar neurons the axons extend correctly to the medullar neuropil.

The hyperplasia seen in AxnS044230 intestinal stem cell clones in the adult midgut is completely suppressed if they are also expressing panΔN.Scer\UAS under the control of Scer\GAL4tub.

Expression of Apc2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4da.G32 has no effect on the ventral denticle belt phenotype of the germ-line and zygotic AxnS044230-mutation.

Expression of Apc2Scer\UAS.T:Src64B,T:Zzzz\FLAG under the control of Scer\GAL4da.G32 has no effect on the ventral denticle belt phenotype of the germ-line and zygotic AxnS044230-mutants.

Triple mutants for AxnS044230, ApcQ8 and Apc2d40 have naked ventral embryonic epidermis.

The ventral denticle belt phenotype of AxnS044230, ApcQ8, Apc2d40 triple mutants is suppressed by expression of AxnScer\UAS.cHa under the control of Scer\GAL4da.G32.

Expression of AxnScer\UAS.T:Src64B,T:Zzzz\FLAG under the control of Scer\GAL4da.G32 has no effect on the ventral denticle belt phenotype of AxnS044230, ApcQ8, Apc2d40 triple mutants.

Expression of Apc2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4da.G32 has no effect on the ventral denticle belt phenotype of AxnS044230, ApcQ8, Apc2d40 triple mutants.

Expression of Apc2Scer\UAS.T:Src64B,T:Zzzz\FLAG under the control of Scer\GAL4da.G32 has no effect on the ventral denticle belt phenotype of AxnS044230, ApcQ8, Apc2d40 triple mutants.

The Scer\GAL4vg.PU-driven gsktScer\UAS.cKa-overexpression phenotype is suppressed by AxnS044230/+ in the wing.

The loss of segmentation and lack of smooth cuticle due to wgl-17/wgl-17 is partially maternally suppressed by AxnS044230/AxnS044230. If these embryos are also zygotically AxnS044230/AxnS044230, they produce cuticles consisting entirely of smooth cuticle (apart from the T1 beard), just as they would if they were wild-type for wg. The denticle belts formed in sggM11; armF1α maternal/zygotic double mutant embryos are largely suppressed by AxnS044230/AxnS044230, leaving mainly naked cuticle without much sign of periodic character.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of AxnScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS fully restores complete and normal denticle belts in 80% of AxnS044230 mutant embryos.

Expression of AxnM2.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS fully restores complete and normal denticle belts in only 59% of AxnS044230 mutant embryos.

Expression of AxnM4.Scer\UAS.T:Avic\GFPunder the control of Scer\GAL4arm.PS fully restores complete and normal denticle belts in only 36% of AxnS044230 mutant embryos.

Expression of AxnM3.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Antp.PU restores complete and normal denticle belts in only 10% of AxnS044230 mutant embryos. Expression of AxnScer\UAS.T:Avic\GFP under the control of Scer\GAL4Antp.PU rescues the denticle phenotype in 32% of AxnS044230 mutant embryos.

All AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnL447Q.αTub84B.T:Zzzz\FLAG die prior to hatching.

Nearly all of the AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnL447S.αTub84B.T:Zzzz\FLAG die prior to hatching.

Most of the AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG hatch successfully.

More than half of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnH504L.αTub84B.T:Zzzz\FLAG hatch successfully.

About half of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnL501P.αTub84B.T:Zzzz\FLAG hatch successfully.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnL501P.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnH504L.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnL447S.αTub84B.T:Zzzz\FLAG shows loss of denticles.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnL447Q.αTub84B.T:Zzzz\FLAG shows loss of denticles.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnH504L.αTub84B.T:Zzzz\FLAG survive to adulthood without defects. Subsequently, if the rescued animals are mated with each other, they produce a few larvae and misshapen pupae that subsequently die. This indicates that both sexes of the parents are fertile.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnL501P.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnK446E.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnL447S.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnL447Q.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG can rescue AxnS044230 mutants to adult viability and normal morphology.

AxnL501P.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG fails to fully rescue AxnS044230 mutants to adult viability. The survival of some of the rescued animals is extended into the pupal stage.

AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnK446E.αTub84B.T:Zzzz\FLAG can fully rescue AxnS044230.

Homozygous AxnS044230 germ-line clone-derived embryos expressing AxnScer\UAS.cHa under the control of Scer\GAL4da.G32 show supernumerary denticles in the ventral embryonic epidermis.

Expression of AxnScer\UAS.T:Src64B,T:Zzzz\FLAG under the control of Scer\GAL4da.G32 can restore ventral denticle belt patterning to some extent of homozygous AxnS044230 germ-line clone-derived embryos.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔRGS.Arm.αTub84B.T:Zzzz\FLAG lack all denticles.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔPP2A.DIX.αTub84B.T:Zzzz\FLAG show the loss of smooth cuticle.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔDIX.αTub84B.T:Zzzz\FLAG exhibit deletions of smooth cuticle bands and the appearance of ectopic denticles.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔPP2A.αTub84B.T:Zzzz\FLAG exhibit deletions of smooth cuticle bands and the appearance of ectopic denticles.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔArm.αTub84B.T:Zzzz\FLAG shows a loss of denticles, including a frequent loss of row 1 denticles.

About half of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔArm.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

The cuticle of AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔSgg.αTub84B.T:Zzzz\FLAG shows a marked loss of denticles.

AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnΔI.αTub84B.T:Zzzz\FLAG display a wild-type pattern of denticle belts.

The majority of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔI.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

Maternal expression of AxnΔRGS.αTub84B.T:Zzzz\FLAG in AxnS044230 mutant (maternal-/zygotic-) embryos almost completely restores the wild-type pattern of denticle belts, although a few row 1 denticles are frequently missing.

The majority of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔRGS.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

Maternal expression of AxnαTub84B.T:Zzzz\FLAG in AxnS044230 mutant (maternal-/zygotic-) embryos restores the wild-type pattern of denticle belts. No ectopic denticles are present. Maternal expression of AxnS044230 allows AxnS044230 (maternal-/zygotic-) embryos to hatch.

Heteroallelic combination of AxnΔRGS.αTub84B.T:Zzzz\FLAG and AxnΔArm.αTub84B.T:Zzzz\FLAG rescues AxnS044230 mutants (maternal-/zygotic-) to viability. Cuticle preparations of the rescued embryos reveal a wild-type pattern of denticle band segmentation with only the occasional loss of denticles in row 1. The rescued flies exhibit normal wing formation without any abnormal loss or gain of margin bristles.

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Notes on Origin
Discoverer
Comments
Comments

Precise excision of the P{lacW} element reverts the lethal phenotype.

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Synonyms and Secondary IDs (12)
References (33)