Flies simultaneously expressing ApplScer\UAS.cTa and BaceScer\UAS.cCSa under the control of Scer\GAL4VT064246 display deficit in both middle- and long-term memory and this can be rescued by co-expression of Nep1EY21255.
The impaired middle- or long-term term memory, MTM or LTM, tested in an aversive olfactory memory assay 2hr or 24hr post-training, respectively, characteristic for adult flies expressing Nep1GD11797 under the control of either Scer\GAL4c739 or Scer\GAL4VT064246 (combined with tub-Gal80[ts] to limit expression to adulthood) is not by enhanced by co-expression of ApplAβ.Scer\UAS.T:Ivir\HA1 with the Scer\GAL4c739 driver, but significantly worsens the MTM phenotype with the Scer\GAL4VT064246 driver. Flies simultaneously expressing Nep1GD11797 and ApplAβ.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4VT064246 do not show any learning ability defects. Both the MTM and the LTM deficit observed in flies expressing Nep1GD11797 under Scer\GAL4VT064246 are exacerbated further by co-expression of BaceScer\UAS.cCSa.
Expression of one copy of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 in a APP-BP1Ex62/+ background results in loss of thoracic microchaetae and a reduction in scutellum size. This phenotype is more severe at 29[o]C than at 25[o]C.
The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of Df(3L)GN24. The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of roblK/+.
Suppresses the leg shaking phenotype of Sh14 when expression is driven by either Scer\GAL4Appl.G1a or Scer\GAL4elav-C155. Suppression is initially complete and starts to decrease on the fourth day after eclosion. Older flies exhibit the non-shaking phenotype for a short time (2-3 minutes) immediately after anesthesia, after which they start to shake at intervals.
Co-expression of BacA\p35Scer\UAS.cHa suppresses the loss of thoracic microchaetae and reduction in scutellum size that is seen in animals expressing one copy of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 in a APP-BP1Ex62/+ background.
37.9% viability when Btau\MAPTScer\UAS.cTa is driven by Scer\GAL4Appl.G1a in combination with ApplScer\UAS.cTa. In all cases lethality acts mostly during the pharate adult stage. Eclosing adults show an infantile phenotype where wing expansion and cuticle hardening did not occur. Transport of axonal cargo is reduced in the Btau\MAPTScer\UAS.cTa, ApplScer\UAS.cTa double mutant.