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General Information
Symbol
Dmel\ApplUAS.cTa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Torroja
FlyBase ID
FBal0097416
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-APPL
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a full length Appl cDNA is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing ApplUAS.cTa under the control of Scer\GAL4GMR91H05 does not lead to pruning or synaptic transmission defects (10 trains of 10 stimulations at 100 Hz) in adult giant fibers.

Expression of ApplScer\UAS.cTa under the control of Scer\GAL4VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) does not impair either middle- or long-term memory compared to controls (tested in an aversive olfactory memory assay 2hr and 24hr post-training, respectively).

Expression of wild-type ApplScer\UAS.cTa in the mushroom bodies, under the RU-fed control of Scer\GAL4Mef2.247.Switch does not alter the STM (2hour) of wild-type flies.

Expression of one copy of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 does not result in a mutant thorax phenotype in adults.

Expression of two copies of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 results in loss of thoracic microchaetae.

Expression of one copy of ApplScer\UAS.cTa under the control of Scer\GAL4Bx-MS1096 has no effect on the size of the wing. Expression of two copies of ApplScer\UAS.cTa under the control of Scer\GAL4Bx-MS1096 results in reduced wing size and loss of crossveins.

Expressing ApplScer\UAS.cTa, under the control of Scer\GAL4P2.4.Pdf, has no effect on the early development of the axonal pattern of sLNv neurons. In contrast, this expression results in a large increase in the area of the axonal arbour in adult flies.

When ApplScer\UAS.cTa is driven by Scer\GAL4sca-537.4 no phenotype is seen.

ApplScer\UAS.cTa alone, when driven by Scer\GAL4Appl.G1a, causes 67% viability. The inviable flies do not show the infantile phenotype, in general. Similar phenotypes occur with the Scer\GAL4elav-C155 driver.

When expression is driven in the motoneurons extra small boutons of abnormal morphology develop. Many small satellite boutons bud off from a central bouton of normal appearance. Molecular markers suggest that the extra boutons are sufficiently normal to form functional synapses. When expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Other
Phenotype Manifest In
Suppressed by
Enhancer of
Suppressor of
Statement
Reference

Scer\GAL4Appl.G1a/ApplUAS.cTa is a suppressor of phenotype of Sh14

Scer\GAL4elav-C155/ApplUAS.cTa is a suppressor of phenotype of Sh14

Other
Additional Comments
Genetic Interactions
Statement
Reference

Flies simultaneously expressing ApplScer\UAS.cTa and BaceScer\UAS.cCSa under the control of Scer\GAL4VT064246 display deficit in both middle- and long-term memory and this can be rescued by co-expression of Nep1EY21255.

The impaired middle- or long-term term memory, MTM or LTM, tested in an aversive olfactory memory assay 2hr or 24hr post-training, respectively, characteristic for adult flies expressing Nep1GD11797 under the control of either Scer\GAL4c739 or Scer\GAL4VT064246 (combined with tub-Gal80[ts] to limit expression to adulthood) is not by enhanced by co-expression of ApplAβ.Scer\UAS.T:Ivir\HA1 with the Scer\GAL4c739 driver, but significantly worsens the MTM phenotype with the Scer\GAL4VT064246 driver. Flies simultaneously expressing Nep1GD11797 and ApplAβ.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4VT064246 do not show any learning ability defects. Both the MTM and the LTM deficit observed in flies expressing Nep1GD11797 under Scer\GAL4VT064246 are exacerbated further by co-expression of BaceScer\UAS.cCSa.

Expression of one copy of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 in a APP-BP1Ex62/+ background results in loss of thoracic microchaetae and a reduction in scutellum size. This phenotype is more severe at 29[o]C than at 25[o]C.

Expression of one copy of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 in a lin19EX/+ background results in loss of thoracic microchaetae.

Coexpression of ApplScer\UAS.cTa and Psn+14.Scer\UAS under the control of Scer\GAL4Act5C.PI does not cause lethality or affect wing patterning.

When numb15 somatic mutant clones are made in the thorax of animals expressing ApplScer\UAS.cTa (driven by Scer\GAL4sca-537.4), mechanosensory organs are seen that are made up of four socket cells.

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of Df(3L)GN24. The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of roblK/+.

The organelle clogging phenotype seen in mutant larvae (driven by Scer\GAL4Appl.G1a) is suppressed by the addition of Df(3L)GN24 or roblK/+.

Suppresses the leg shaking phenotype of Sh14 when expression is driven by either Scer\GAL4Appl.G1a or Scer\GAL4elav-C155. Suppression is initially complete and starts to decrease on the fourth day after eclosion. Older flies exhibit the non-shaking phenotype for a short time (2-3 minutes) immediately after anesthesia, after which they start to shake at intervals.

Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cHa suppresses the loss of thoracic microchaetae and reduction in scutellum size that is seen in animals expressing one copy of ApplScer\UAS.cTa under the control of Scer\GAL4ap-md544 in a APP-BP1Ex62/+ background.

37.9% viability when Btau\MAPTScer\UAS.cTa is driven by Scer\GAL4Appl.G1a in combination with ApplScer\UAS.cTa. In all cases lethality acts mostly during the pharate adult stage. Eclosing adults show an infantile phenotype where wing expansion and cuticle hardening did not occur. Transport of axonal cargo is reduced in the Btau\MAPTScer\UAS.cTa, ApplScer\UAS.cTa double mutant.

Complementation and Rescue Data
Partially rescues
Comments

Expression of wild-type ApplScer\UAS.cTa in the mushroom bodies, under the RU-fed control of Scer\GAL4Mef2.247.Switch rescues the STM and LTM deficits found in Appld/+ ; Scer\GAL4Mef2.247.Switch flies.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ApplScer\UAS.cTa
ApplUAS.cTa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Torroja
Secondary FlyBase IDs
    References (12)