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General Information
Symbol
Dmel\sggUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Bourouis
FlyBase ID
FBal0097905
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-sgg, UAS-GSK3, UAS-SGG10, UAS-shaggy
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of sgg coding sequence is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

adult thorax & chaeta, with Scer\GAL4sca-537.4

adult thorax & microchaeta, with Scer\GAL4sca-537.4

Detailed Description
Statement
Reference

Adult males and females expressing sggUAS.cBa under the control of Scer\GAL4elav-C155 display severely impaired locomotor activity.

Both males and females expressing sggUAS.cBa under the control of Scer\GAL4Toll-6-D42 display significantly increased locomotor activity at day 20 of adulthood, even though younger adults (at 3-5 days post eclosion) have similar levels of activity as controls. At day 40 a significant drop in activity is detected in males, while females display similar levels as controls.

Third instar larvae expressing sggUAS.cBa under the control of either Scer\GAL4elav-C155 or Scer\GAL4Toll-6-D42 have significantly decreased numbers of mitochondria at the neuromuscular junction, clustered predominantly at NMJ boutons (and not evenly distributed along terminal arbors as in wild-type controls), a disrupted microtubule cytoskeleton (as determined by futsch immunostaining) and a significantly decreased number of brp-immunopositive presynaptic active zones compared to controls.

Adult flies expressing sggUAS.cBa under the control of Scer\GAL4elav-C155 show signs of neurodegeneration with swollen, dense mitochondria in the inferior dorsofrontal protocerebrum; "holes" surrounded by membranes containing, in some cases, fragments of organelles apparently subjected to autophagy in the brain; and rhabdomeres with lacunae and irregular structures. Additionally, flies display a high variety of wing shapes.

The expression of sggUAS.cBa under the control of Scer\GAL4nub.PU leads to extremely small and deformed wings.

Expression of sggScer\UAS.cBa in the mushroom bodies under the control of Scer\GAL4Tab2-201Y or Scer\GAL4Mef2.247 causes an increase in the size of the mushroom body lobes and calyces, compared with controls. These synaptic changes do not have a detectable consequence on odour central adaptation to EB, IAA and benzaldehyde.

Expression of sggScer\UAS.cBa in the projection neurons, under the control of Scer\GAL4GH146 or Scer\GAL4exba-krasavietz, does not affect central or cross adaptation to EB, IAA and benzaldehyde odours.

Expression of sggScer\UAS.cBa under the control of either Scer\GAL4GMR.PF or Scer\GAL4ey.PH results in uniform lethality. Individuals that survive to the pharate adult stage lack significant eye or related neural head structures.

Expression of sggScer\UAS.cBa under the control of Scer\GAL4C-765 does not result in a loss of bristles on the notum, but the posterior dorsocentral bristles are approximately 15% shorter than normal.

Expression of sggScer\UAS.cBa in 30-32 neurons of the antennal lobe under the control of Scer\GAL4GH298 reduces neuronal branching. There are no significant changes in cell number in these mutants.

Expression of sggScer\UAS.cBa in 30-32 neurons of the antennal lobe under the control of Scer\GAL4GH298 leads to odorant perception changes. Response to benzaldehyde, ethyl hexanoate, ethyl butyrate, and propionic acid shifts towards repulsion. The strength of repulsive reaction is proportional to the odorant concentration. Inhibition of sggScer\UAS.cBa expression until the first day of adult life (through the presence of Scer\GAL80ts.αTub84B) does not alter odorant perception changes compared to flies with chronic sggScer\UAS.cBa expression.

Expression of sggScer\UAS.cBa in 6-8 neurons of the antennal lobe under the control of Scer\GAL4exba-krasavietz reduces neuronal branching. There are no significant changes in cell number in these mutants.

Expression of sggScer\UAS.cBa in 6-8 neurons of the antennal lobe under the control of Scer\GAL4exba-krasavietz leads to odorant perception changes. Response to benzaldehyde, ethyl hexanoate, ethyl butyrate, and propionic acid shifts towards attraction. The strength of attractive reaction is proportional to the odorant concentration. A Scer\GAL80Cha.3.3P background completely abolishes this attraction.

Expression of sggScer\UAS.cBa in about 18 intrinsic neurons in the antennal lobe under the control of Scer\GAL4NP1227 results in synapse loss but no detectable effect in odorant perception. Simultaneous loss of synapses through expression of sggScer\UAS.cBa under the control of both Scer\GAL4NP1227 and Scer\GAL4exba-krasavietz exhibits the typical effects due to expression in Scer\GAL4exba-krasavietz neurons alone.

Expression of sggScer\UAS.cBa in about 37 intrinsic neurons in the antennal lobe under the control of Scer\GAL4NP2426 results in synapse loss and a shift in perception, with responses to odorants becoming more repulsive. The simultaneous alteration of both Scer\GAL4NP2426 and Scer\GAL4exba-krasavietz neurons restores normal perception.

Up-regulation of sgg through expression of sggScer\UAS.cBa under the control of Scer\GAL4Tab2-201Y causes a substantial reduction of neural branching in its MB domain. However, odorant perception is not affected in these mutants.

Overexpression of sggScer\UAS.cBa in the eye under the control of Scer\GAL4GMR.PFa results in a mild rough eye phenotype.

Presynaptic expression of sggScer\UAS.cBa with Scer\GAL4C380 completely suppresses activity-dependent induction of ghost boutons when stimulated with 5X K[+] spaced depolarisation. These conditions also result in an increase in mEJP (spontaneous excitatory potentials) frequency, though this increase is less than two-fold compared with a 3-fold enhancement in wildtype larvae.neurophysiology defective | larval stage

Expression of sggScer\UAS.cBa under the control of Scer\GAL4Act88F.PD does not result in a mutant wing phenotype.

Files expressing sggScer\UAS.cBa under the control of Scer\GAL4tim.Scer\UAS have a locomotor activity period approximately 5 hours shorter than that of controls.

Ectopic expression of sggScer\UAS.cBa in the wing (under the control of Scer\GAL430A) causes failure of epithelial cells to delaminate from the cuticle and dorsal and ventral cuticle surfaces do not bond.

Scer\GAL4He.PZ-driven expression of sggScer\UAS.cBa has no effect on removal of the epithelial cells from the wing or the bonding of the wing surfaces.

Overexpression of sggScer\UAS.cBa in clock cells under the control of Scer\GAL4tim.PE results in a shortening of circadian period.

Overexpression of sggScer\UAS.cBa in post-mitotic neurons under the control of Scer\GAL4elav-C155 results in increased olfactory habituation.

Overexpression of sggScer\UAS.cBa under the control of Scer\GAL4D42 dramatically reduces synapse number in fibers F12 and F13.

Overexpression of sggScer\UAS.cBa under the control of Scer\GAL4796 significantly reduces the synaptic domain in the ellipsoid body.

Overexpression of sggScer\UAS.cBa under the control of Scer\GAL4tim.PE produces a period of approximately 18 hours.

Expression of sggScer\UAS.cBa under the control of Scer\GAL4bi-md653 results in flies with severely atrophied wings. Expression of sggScer\UAS.cBa under the control of Scer\GAL4vg.PM results in nicking of the wing margin and missing wing margin bristles. Expression of sggScer\UAS.cBa under the control of Scer\GAL4455.2 results in a loss of scutellar bristles. A variable cleft notum phenotype and reduction in size of the scutellum is also seen. The precursors of the scutellar bristles are absent in 3rd larval instar wing discs expressing sggScer\UAS.cBa under the control of Scer\GAL4455.2. Expression of sggScer\UAS.cBa under the control of Scer\GAL4sca-537.4 results in a loss of thoracic chaetae, with the remaining microchaetae being thin and tiny. Wing margin bristles are fewer in number and smaller than wild type.

When sggEP1576 is driven by Scer\GAL4tim.Scer\UAS or Scer\GAL4tim.PE the locomotor activity period is decreased by approximately 3 hours. Longevity is also reduced upon expression driven by Scer\GAL4tim.Scer\UAS.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

sggJ11 suppresses the degeneration seen in leg motor neuron clones expressing Hsap\TARDBPQ331K.Scer\UAS.cSa under the control of Scer\GAL4VGlut-OK371. This suppression is reversed upon expression of sggScer\UAS.cBa or in the presence of Dp(1;3)DC048.

CRMPsupK1 homozygotes expressing sggScer\UAS.cBa under the control of either Scer\GAL4GMR.PF or Scer\GAL4ey.PH regularly survive to the pharate adult stage and frequently eclose as adults displaying reduced rough eyes.

Co-expression of sggScer\UAS.cBa enhances the gnarled wing phenotype caused by expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD.

Files coexpressing sggScer\UAS.cBa and NiPp1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4tim.Scer\UAS have an average locomotor activity period of 19.5 hours, approximately 1.3 hours longer than that of flies expressing sggScer\UAS.cBa alone under the control of Scer\GAL4tim.Scer\UAS.

Co-expression of 5-HT1BScer\UAS.cYa with sggScer\UAS.cBa, both under the control of Scer\GAL4tim.PE, lengthens the 18 hour period observed in sggScer\UAS.cBa mutants to 19.7 hours.

Xenogenetic Interactions
Statement
Reference

Expression of sggScer\UAS.cBa enhances the toxicity of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies (when both lines are expressed in the eye under the control of Scer\GAL4GMR.long. Flies expressing one copy of both lines exhibit serious eye depigmentation, including a greater loss of pigment cells, rough eye collapse, and a highly disordered ommatidial array. These defects are exacerbated by an additional copy of the sggScer\UAS.cBa transgene.

Co-expression of sggScer\UAS.cBa leads to lethality in flies with expression of Hsap\HTTQ93.ex1p.Scer\UAS driven by Scer\GAL4elav.PU.

Co-expression of sggScer\UAS.cBa with Zzzz\CTG86.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PFa is completely lethal.

Co-expression of sggScer\UAS.cBa with Zzzz\CAG93.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PFa results in an increase in the eye compared with ectopic expression of sggScer\UAS.cBa alone. There appears to be a reduction in the amount of pigment in the eye and in most cases there are necrotic patches and nearly complete loss of ommatidial array structure.

Co-expression of sggScer\UAS.cBa with Zzzz\CAGCAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PFa results in an increase in the eye compared with ectopic expression of sggScer\UAS.cBa alone. There appears to be a reduction in the amount of pigment in the eye and in most cases there are necrotic patches and nearly complete loss of ommatidial array structure.

Co-expression of sggScer\UAS.cBa with Zzzz\CTGterm.CTG.99.Scer\UAS under the control of Scer\GAL4GMR.PFa is completely lethal at 25[o]C. When flies are grown at 23[o]C to reduce expression levels of the Scer\UAS constructs, a severe phenotype involving loss of pigmentation, necrotic patches and a loss of ommatidial organisation of the eye is observed.

Overexpression of sggScer\UAS.cBa under the control of Scer\GAL4GMR.PFa and in the presence of Zzzz\CAGterm93.Scer\UAS results in a decrease in the amount of pigmentation in the eye compared with overexpression of sggScer\UAS.cBa alone. There is a marked increase in the degree of roughness of the surface of the eye in flies co-expressing sggScer\UAS.cBa and Zzzz\CAGterm93.Scer\UAS.

Overexpression of sggScer\UAS.cBa under the control of Scer\GAL4GMR.PFa and in the presence of Zzzz\CAGterm.CAA.114.Scer\UAS results in a decrease in the amount of pigmentation in the eye compared with overexpression of sggScer\UAS.cBa alone.

Overexpression of sggScer\UAS.cBa under the control of Scer\GAL4GMR.PFa and in the presence of Hsap\ATXN10rAUUCU.65.Scer\UAS results in a decrease in the amount of pigmentation in the eye compared with overexpression of sggScer\UAS.cBa alone. There is a marked increase in the degree of roughness of the surface of the eye in flies co-expressing sggScer\UAS.cBa and Hsap\ATXN10rAUUCU.65.Scer\UAS.

sggScer\UAS.cBa fails to suppress the toxic effects of Scer\GAL4ppk.PG>Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG on microtubule fragmentation in dendritic processes.

Co-expression of sggScer\UAS.cBa in Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG-expressing neurons by Scer\GAL4Ddc.PL maintains the disrupted dendritic structure in 2-week-old adult brains, similar to expression of Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG alone.

Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
sggScer\UAS.cBa
sggUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Bourouis
Secondary FlyBase IDs
    References (32)