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General Information
Symbol
Dmel\upd1UAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zeidler
FlyBase ID
FBal0097930
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-upd, UAS-Os, UAS-Unpaired
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of upd1.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of upd1Scer\UAS.cZa driven by Scer\GAL4GMR.PU causes enlarged eyes in pharate adults.

Scer\GAL4ey.PH-mediated expression results in tumourous eyes.

Expression of hopTum.Scer\UAS under the control of Scer\GAL4byn-Gal4 results in stage 15 embryonic hindguts having a lower angle of curvature, as compared to controls.

Expression of upd1Scer\UAS.cZa in wild-type tissue results in a mild overgrowth phenotype.

Expression of upd1Scer\UAS.cZa using Scer\GAL4GMR.PU increases eye size.

Expression of upd1Scer\UAS.cZa using Scer\GAL4upd1-E132 or Scer\GAL4slbo.2.6 results in delayed, supernumerary border cells.

Over-expression of upd1Scer\UAS.cZa in MARCM mutant clones (under the control of Scer\GAL4esg-NP5130) induces intestinal stem cell proliferation.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4ey.PH results in enlarged adult eyes.

Expression of upd1Scer\UAS.cZa in somatic cells under the control of Scer\GAL4A3.eya (restricted to adult stages using Scer\GAL80ts.αTub84B) results in testes that are filled with undifferentiated cells. Small early stage germ cells are intermingled with excess somatic cells.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, in a bgcnQS2 mutant background results in excess germ cells that contain dot fusomes and are thus germline stem cells or gonialblasts. These testes also have a large accumulation of early cyst cells, many of which are likely to be cyst progenitor cells.

In upd1Scer\UAS.cZa; Scer\GAL4C587 ovarioles, there are increased numbers of germline stem cells, escort stem cells and germline cysts compared to wild-type and germaria become disorganised. The diameter of the anterior germarium increases in these ovarioles so that more that two cysts (the usual limit) span it. Frequently these ovarioles have two tips and two parades of downstream cysts within a single sheath. In rare cases ovarioles become completely filled with cells which resemble germline stem cells.

Expression of upd1Scer\UAS.cZa in border cells, driven by Scer\GAL4slbo.2.6, causes ectopic outer border cells to form. However, the border cell cluster that is also present in wild-type egg chambers migrates normally.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in proliferation of germ cells in XX embryos (in contrast to wild type, where germ cells do not proliferate in XX embryos).

upd1Scer\UAS.cZa expression in the eye under the control of Scer\GAL4ey.PH results in a larger eye compared to wild-type. In third instar larvae, the eye disc is enlarged in size along the dorsal-ventral axis.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4dpp.blk1 results in an enlarged third instar eye disc along the dorsal-ventral axis. In the adult the eye is enlarged, with stronger overgrowth observed in the dorsal than in the ventral eye.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4bi-omb-Gal4 results in an enlarged eye primarily along the dorsal-ventral axis.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4GMR.PF results in an enlarged eye, with stronger overgrowth observed in the dorsal than in the ventral eye. The number of mitotic cells in the third instar larval eye disc increase significantly before the morphogenetic furrow. The number of R8 photoreceptor cells is increased, as is the number of ommatidium. The cell-composition of these additional ommatidium is wild-type.

Expression of upd1Scer\UAS.cZa in somatic clones in the eye under the control of Scer\GAL4Act5C.PI results in non-autonomous overgrowth of the eye-disc.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4ey.PH, Scer\GAL4elav.PLu, Scer\GAL4GMR.PF or Scer\GAL4dpp.blk1 results in flies with enlarged eyes. In all cases, the enlarged eyes have prominent outgrowths, primarily in the dorsal portion of the eye.

Embryos expressing upd1Scer\UAS.cZa under the control of Scer\GAL4da.G32 show abnormal retraction of the germ band and have a dorsal hole, indicating abnormal development of the amnioserosa.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4c381.661 results in a dorsal hole phenotype.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4byn-Gal4 results in embryos with shorter and wider hindguts than normal. Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4byn-Gal4 in a Df(1)os1A2 background does not rescue the hindgut, but results in a severely defective, short and wide hindgut.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4twi.PG causes the aggregation of pole cells in ectopic locations. Expression driven by Scer\GAL4elav-C155 causes the mislocalization of some pole cells into the nerve cord, while expression driven by Scer\GAL4prd.RG1 causes ectopic localization of pole cells in alternate segments of the embryo.

Somatic clones of upd1Scer\UAS.cZa; Scer\GAL4Act5C.PP in anterior ovarian follicle cells cause the formation of ectopic border cells, both within and adjacent to the clone. This effect is not seen when such clones are present in posterior ovarian follicle cells. Somatic clones of upd1Scer\UAS.cZa; Scer\GAL4Act5C.PP induce main body (oocyte associated) follicle cells to express a centripetal follicle cell marker. This effect is graded: strongest in anterior main body follicle cells; weaker in clones of follicle cells sitting over the middle of the oocyte; and absent in posterior clones.

When upd1Scer\UAS.cZa is driven by Scer\GAL4slbo.2.6, the number of migratory border cells appears to be the same, but appears to induce some border cell markers in a non-cell autonomous manner.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4slbo.2.6 results in an increased number of vesicles in the border follicle cells compared to wild type and also results in an increased number of border follicle cells.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4h-1J3 results in abnormal head development in 81% of embryos.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in greatly enlarged testes filled with many small cells. Many of the cells contain a spherical spectrosome, and many divide asynchronously, suggesting stem cell or gonialblast identity. Cells with branched fusomes are occasionally seen. The number of tj-positive cyst cells is also increased, suggesting proliferation of somatic cyst progenitor cells (CPCs) or early cyst cells. The apical hub remains intact.

When upd1Scer\UAS.cZa is driven by Scer\GAL4slbo.2.6 and Scer\GAL4c306, numerous slbo expressing border cells are seen compared to wild-type. Some of the extra border cells migrate as single cells, whereas others form multiple small clusters and still others form one large cluster. High levels of ectopic upd1Scer\UAS.cZa results in egg chambers in which both normal and extra border cells frequently fail to migrate.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 or Scer\GAL4Act5C.PP results in a striking accumulation of early-stage cells distant from the hub in the testis. By their morphology and DNA staining characteristics, these cells are a mixture of undifferentiated germ line cells (germline stem cells and/or gonialblasts) and somatic cells (somatic cyst progenitor cells). This is also supported by the increased number of cells containing spherical fusomes (characteristic of germline stem cells and gonialblasts) compared to wild type. The number of bam-positive spermatogonia is also increased. Somatic cells are seen encircling germline stem cell and/or gonialblast cell pairs (similar to the situation seen in wild type).

Homozygous clones in the eye expressing upd1Scer\UAS.cZa under the control of Scer\GAL4Act5C.PI result in inversion of ommatidial polarity in the wild-type tissue on the equatorial side of the clone. The greatest effect is seen in clones closer to the poles of the eye disc. Expression of upd1Scer\UAS.cZa at the poles of the eye using Scer\GAL430A results in the disruption of the regular array of ommatidia at the dorsal and ventral poles of the eye. Ommatidial polarity is normal at the equator of the eye in these flies, but ommatidial orientation is inverted at the poles. Within this region of ommatidial polarity inversion there is no clear boundary between dorsal and ventral ommatidial fates.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4upd1.PU fails to rescue the germline stem cell, cyst stem cell, and hub cell number phenotypes in testicular niches of flies expressing sdaE-A.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU in sdaiso7.8/Df(3R)ED6235 mutants, and has no significant effect on cell numbers in testicular niches of flies expressing sdaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4upd1.PU in sdaiso7.8/Df(3R)ED6235 mutants.

Ipk220B/Ipk220B suppresses the enlarged eyes seen in pharate adults with expression of upd1Scer\UAS.cZa driven by Scer\GAL4GMR.PU.

His2Av810/Df(3R)BSC524 does not suppress the excess of germline stem cell (GSC)-like cells seen in larval testes when upd1Scer\UAS.cZa is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, although a few germ cell cysts with branched fusomes are seen.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4Act5C.PU in flwFP41 mutant follicle cell clones results in overproliferation in anterior and lateral follicle cells. The amount of cell overproliferation in posterior follicle cells is increased compared to flwFP41 clones alone.

Overexpression of upd1Scer\UAS.cZa in Ras85DV12.Scer\UAS MARCM clones generates larger tumorigenic overgrowths than in either single mutant clone.

Knock-down of yki through co-expression of ykidsRNA.N.Scer\UAS in upd1Scer\UAS.cZa MARCM mutant clones (under the control of Scer\GAL4esg-NP5130) does not affect intestinal stem cell proliferation.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4ey.PH in a Lfee mutant background exacerbates the Lfee phenotype and results in eyeless heads.

While overexpression of upd1Scer\UAS.cZa in the eye gives rise to enlarged eyes, it is not sufficient to suppress hidGMR.PG.

Simultaneous expression of upd1Scer\UAS.cZa, emsScer\UAS.cSa, ctScer\UAS.cLa and grnScer\UAS.cBa, under the control of Scer\GAL469B allows formation of spiracle-like tubular structures in Abd-BM1/Abd-BM1 embryos.

upd1Scer\UAS.cZa; Scer\GAL4dpp.blk1 completely restores eye development in eyg1/eygM3-12 flies.

The large eye-phenotype observed in flies expressing upd1Scer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed in a Stat92E06346/+ background.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4drm.7.1 significantly rescues the drm3/drm6 mutant hindgut phenotype; the hindgut is more elongated and has fewer cells in its circumference than in drm3/drm6 embryos.

Co-expression of domeΔCYT.Scer\UAS completely suppresses the extra border follicle cells produced by expression of upd1Scer\UAS.cZa under the control of Scer\GAL4slbo.2.6.

Abnormal head development is seen in only 16% of embryos expressing upd1Scer\UAS.cZa under the control of Scer\GAL4h-1J3 which are also mutant for domeunspecified.

Xenogenetic Interactions
Statement
Reference

Ipk220B/Ipk220B suppresses the enlarged eyes seen in pharate adults with expression of upd1Scer\UAS.cZa driven by Scer\GAL4GMR.PU, even with co-expression of BacA\p35Scer\UAS.cUa.

Complementation and Rescue Data
Comments

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 rescues the germ-band extension and apical cell shape defects observed in upd1YM55 mutant embryos.

Expression of upd1Scer\UAS.cZa in the eye under the control of Scer\GAL4ey.PH fully rescues the small-eye phenotype of Df(1)os1 mutant animals.

Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4ey.PH rescues the small eye phenotype of upd1unspecified/Df(1)os1A2 flies.

Embryos expressing upd1Scer\UAS.cZa under the control of Scer\GAL4drm.7.1 in a Df(1)os1A2 background appear to have morphologically normal hindguts, which have an average length about 93% that of wild type. Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4byn-Gal4 in a Df(1)os1A2 background does not rescue the hindgut, but results in a severely defective, short and wide hindgut. Expression of upd1Scer\UAS.cZa under the control of Scer\GAL4fkh.14-3 or Scer\GAL4455.2 does not rescue the Df(1)os1A2 mutant hindgut phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
osScer\UAS.cZa
upd1Scer\UAS.cZa
upd1UAS.cZa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Zeidler
Secondary FlyBase IDs
    References (53)